We narrowed to 9,802 results for: otos

1. pRSFDuet-sumo-h-cGAS

   Plasmid

   #127161

   Purpose

   expression of human cGAS protein

   Depositor

   Thomas Tuschl

   Article

   Lama et al Nat Commun. 2019 May 21;10(1):2261. doi:

   Insert

   MB21D1 (CGAS Human)
   

   Use

   Tags

   His-SUMO tag

   Expression

   Bacterial

   Mutation

   None

   Promoter

   Available since

   Oct. 2, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. iGFP-gamma-actin

   Plasmid

   #231554

   Purpose

   Mammalian expression of human gamma actin fused intramolecularly to monomeric superfolder GFP

   Depositor

   Manos Mavrakis

   Article

   Martins et al Cell. 2025 Apr 1:S0092-8674(25)00276-4.

   Insert

   ACTG1 (ACTG1 Human)
   

   Use

   Tags

   msfGFP (intramolecular)

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   March 19, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pAAV-nEFCas9

   Plasmid

   #87115

   Purpose

   SpCas9 expression AAV backbone plasmid under control of nEF promoter

   Depositor

   Juan Belmonte

   Article

   Suzuki et al Nature. 2016 Dec 1;540(7631):144-149. do

   Insert

   nEF-Cas9
   

   Use

   AAV and CRISPR

   Tags

   HA-NLS

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   March 30, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pcDNA3.1/Puro-CAG-JEDI-2P

   Plasmid

   #179458

   Purpose

   Genetically encoded voltage indicator (GEVI) JEDI-2P expressed under strong mammalian promoter (CAG)

   Depositor

   Francois St-Pierre

   Article

   Liu et al Cell. 2022 Aug 16. pii: S0092-8674(22)00

   Insert

   JEDI-2P
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CAG

   Available since

   Aug. 15, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pICE-EGFP-FLAG-PLIN3

   Plasmid

   #161918

   Purpose

   Expresses human PLIN3 with a N-terminal GFP-FLAG tag. Confers Puromycin resistance. Inducible in T-REx cells.

   Depositor

   Sébastien Britton

   Article

   Demange et al Elife. 2022 May 10;11. pii: 73913. doi:

   Insert

   Perilipin-3 (PLIN3 Human)
   

   Use

   Tags

   GFP-FLAG

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   May 12, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pMRX-IP-EGFP-PA-BAX(ER)

   Plasmid

   #228941

   Purpose

   Stable expression of EGFP-LOV2(N538E)-BAX-Cyb5a in mammalian cells. Photoactivatable BAX localizes on ER and induces ER rupture upon blue light stimulation.

   Depositor

   Noboru Mizushima

   Article

   Nagashima et al J Biol Chem. 2025 Mar 18;301(4):108421.

   Inserts

   LOV2 domain of phototropin1
   BAX (BAX Human)
   Cyb5a (Cyb5a Rat)
   

   Use

   Retroviral

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   N-terminus and C-terminus are deleted. BAX(3-171)…

   Promoter

   Available since

   April 28, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pKLV2-U6gRNA5(LIN28A_5-4)-PGKpuroBFP-W

   Plasmid

   #211969

   Purpose

   Express gRNA against LIN28A with puro and BFP

   Depositor

   Kosuke Yusa

   Article

   Ishikawa et al Commun Biol. 2023 Dec 28;6(1):1290. doi:

   Insert

   sgRNA targeting LIN28A (LIN28A Human)
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Human U6 promoter

   Available since

   Jan. 24, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pKLV2-U6gRNA5(LIN28A_5-3)-PGKpuroBFP-W

   Plasmid

   #211968

   Purpose

   Express gRNA against LIN28A with puro and BFP

   Depositor

   Kosuke Yusa

   Article

   Ishikawa et al Commun Biol. 2023 Dec 28;6(1):1290. doi:

   Insert

   sgRNA targeting LIN28A (LIN28A Human)
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Human U6 promoter

   Available since

   Jan. 24, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pLL3.7 FLAG-YAP1-TEAD-P-H2B-mCherry

   Plasmid

   #128327

   Purpose

   Reporter to evaluate YAP1/TEAD-mediated gene transcription

   Depositor

   Yutaka Hata

   Article

   Maruyama et al Mol Cancer Res. 2018 Feb;16(2):197-211.

   Inserts

   H2B mCherry
   YAP1 (YAP1 Human)
   

   Use

   Lentiviral

   Tags

   FLAG

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   March 18, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pNCS-mGold2t

    Plasmid

    #231766

    Purpose

    Expression of mGold2t in bacterial cells

    Depositor

    Francois St-Pierre

    Article

    Lee et al Nat Commun. 2025 Apr 4;16(1):3241. doi:

    Insert

    mGold2t
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    mGold2t is mGold with V1A;V22I;H77N;Q80R;K101E;D1…

    Promoter

    Synthetic promoter

    Available since

    March 17, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pCIG-FGF13A

    Plasmid

    #220793

    Purpose

    To express the human FGF13 isoform 1(FGF13A) in mammalian cells together with EGFP

    Depositor

    Pierre Vanderhaeghen

    Article

    Libe-Philippot et al Cell. 2023 Dec 21;186(26):5766-5783.e25.

    Insert

    FGF13 (FGF13 Human)
    

    Use

    Tags

    Expression

    Mutation

    Promoter

    Available since

    July 22, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pKLV-EF1a-mCherryBCL11B-W

    Plasmid

    #208758

    Purpose

    Lentiviral vector expressing mCherry fused with BCL11B cDNA

    Depositor

    Kosuke Yusa

    Article

    Aoki et al Blood. 2023 Nov 3:blood.2023020857. doi:

    Insert

    BCL11B (BCL11B Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Jan. 17, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. CS-TRE-BMI1-Ubc-rtTA

    Plasmid

    #198191

    Purpose

    Generation of Expandable megakaryocyte cell lines from human iPS cells

    Depositor

    Hiromitsu Nakauchi

    Article

    Nakamura et al Cell Stem Cell. 2014 Apr 3;14(4):535-48.

    Insert

    hBMI1 (BMI1 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    May 8, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. CS-TRE-c-Myc-Ubc-rtTA

    Plasmid

    #198190

    Purpose

    Generation of Expandable megakaryocyte cell lines from human iPS cells

    Depositor

    Hiromitsu Nakauchi

    Article

    Nakamura et al Cell Stem Cell. 2014 Apr 3;14(4):535-48.

    Insert

    hC-MYC (MYC Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    May 8, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pRetroX-TetOne-Puro-p62

    Plasmid

    #204548

    Purpose

    Expresses p62 in a doxycycline-dependent manner in mammalian cells

    Depositor

    Masaaki Komatsu

    Article

    Kurusu et al Dev Cell. 2023 May 9:S1534-5807(23)00191

    Insert

    p62 (SQSTM1 Human)
    

    Use

    Retroviral

    Tags

    Expression

    Mammalian

    Mutation

    None

    Promoter

    Available since

    Aug. 11, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pAAV-EF1a-DIO-JEDI-2P-WPRE

    Plasmid

    #179460

    Purpose

    Double floxed genetically encoded voltage indicator (GEVI) JEDI-2P in AAV production vector under the control of the mammalian promoter (EF1a)

    Depositor

    Francois St-Pierre

    Article

    Liu et al Cell. 2022 Aug 16. pii: S0092-8674(22)00

    Has Service

    AAV1

    Insert

    JEDI-2P
    

    Use

    AAV and Cre/Lox

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a

    Available since

    Aug. 15, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pAAV-EF1a-DIO-ASAP3Kv-WPRE

    Plasmid

    #132330

    Purpose

    expresses ASAP3Kv(soma-located version of ASAP3, ASAP-family genetically encoded voltage indicator) in cre recombinase expressing mouse/cell, gene delivered by pAAV-EF1a vector

    Depositor

    Michael Lin

    Article

    Villette et al Cell. 2019 Dec 12;179(7):1590-1608.e23.

    Insert

    ASAP3KV
    

    Use

    AAV and Cre/Lox

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a

    Available since

    Dec. 6, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pIG-804_HA-GD2-28z_CAR_BATF-TFAP4

    Plasmid

    #207491

    Purpose

    This plasmid can be used to generate HDR templates for non-viral knockin into the TRAC locus of human T cells.

    Depositor

    Alexander Marson

    Article

    Blaeschke et al Cell. 2023 Sep 14;186(19):4216-4234.e33.

    Insert

    BATF-TFAP4, HA-GD2-28z_CAR (BATF Human)
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Jan. 26, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pIG-704_CD19-28z_CAR_tNGFR

    Plasmid

    #207486

    Purpose

    This plasmid can be used to generate HDR templates for non-viral knockin into the TRAC locus of human T cells.

    Depositor

    Alexander Marson

    Article

    Blaeschke et al Cell. 2023 Sep 14;186(19):4216-4234.e33.

    Insert

    tNGFR, CD19-28z_CAR (NGFR Human)
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Jan. 26, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pLentiCMVblast_SLC38A2_wt

    Plasmid

    #156180

    Purpose

    CMV-driven expression of sgRNA-resistant SLC38A2 wild-type cDNA.

    Depositor

    Alec Kimmelman

    Article

    Parker et al Cancer Discov. 2020 Apr 27. pii: 2159-82

    Insert

    SLC38A2 (SLC38A2 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Silent mutations to prevent targeting by sgRNA se…

    Promoter

    CMV

    Available since

    Sept. 22, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. HDM-SARS2-Spike-delta21

    Plasmid

    #155130

    Purpose

    Encodes SARS-CoV-2 Spike with 21 aa C-terminal deletions for lentiviral pseudotyping

    Depositor

    Jesse Bloom

    Article

    Crawford et al Viruses. 2020 May 6;12(5). pii: v1205051

    Insert

    SARS-CoV-2 Spike-delta21 (S Severe acute respiratory syndrome coronavirus 2)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Codon optimized to H. sapiens using IDT codon opt…

    Promoter

    CMV

    Available since

    July 21, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. pSIREN-RetroQ-BPTF-sh5

    Plasmid

    #83045

    Purpose

    MMLV LTR plasmid pSIREN-RetroQ-BPTF-sh5

    Depositor

    Joe Landry

    Article

    Mayes et al Oncotarget. 2017 May 12;8(38):64344-6435

    Insert

    shRNA #5 to BPTF (BPTF, Bptf Human, Mouse)
    

    Use

    Retroviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Oct. 24, 2016

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

23. pAAV-hSyn-JEDI-2P-WPRE

    Plasmid

    #179464

    Purpose

    Genetically encoded voltage indicator (GEVI) JEDI-2P in AAV production vector for neuron -specific expression using the promoter hSyn

    Depositor

    Francois St-Pierre

    Article

    Liu et al Cell. 2022 Aug 16. pii: S0092-8674(22)00

    Has Service

    AAV9

    Insert

    JEDI-2P
    

    Use

    AAV

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    hSyn

    Available since

    Aug. 15, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pIG-702_CD19-BBz_CAR_tNGFR

    Plasmid

    #207485

    Purpose

    This plasmid can be used to generate HDR templates for non-viral knockin into the TRAC locus of human T cells.

    Depositor

    Alexander Marson

    Article

    Blaeschke et al Cell. 2023 Sep 14;186(19):4216-4234.e33.

    Insert

    tNGFR, CD19-BBz_CAR (NGFR Human)
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Jan. 26, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pKLV2-U6gRNA5(JARID2_5-4)-PGKpuroBFP-W

    Plasmid

    #211966

    Purpose

    Express gRNA against JARID2 with puro and BFP

    Depositor

    Kosuke Yusa

    Article

    Ishikawa et al Commun Biol. 2023 Dec 28;6(1):1290. doi:

    Insert

    sgRNA targeting JARID2 (JARID2 Human)
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Human U6 promoter

    Available since

    Jan. 24, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. GFP-MYO5C

    Plasmid

    #135404

    Purpose

    Expresses GFP-tagged human MYO5C in mammalian cells.

    Depositor

    Richard Cheney

    Article

    Jacobs et al Mol Biol Cell. 2009 Nov;20(21):4471-88.

    Insert

    MYO5C (MYO5C Human)
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    Relative to the GenBank MYO5C cDNA sequence repor…

    Promoter

    CMV

    Available since

    Dec. 16, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pAAV-EF1α1.1-ChrimsonR-GFP

    Plasmid

    #122063

    Purpose

    AAV-mediated expression of ChrimsonR-GFP under the EF1α promoter (1.1kb short version). Using SV40 pA signal.

    Depositor

    Edward Boyden

    Article

    Klapoetke et al Nat Methods. 2014 Mar;11(3):338-46. doi:

    Insert

    ChrimsonR-GFP
    

    Use

    AAV

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    Chrimson K176R mutant

    Promoter

    EF1a（1.1kb short version)

    Available since

    March 28, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pMXs-HRAS(G12V)-IRES-p53DD

    Plasmid

    #233185

    Purpose

    Retroviral expression of HRAS(G12V) and p53DD (RIDD)

    Depositor

    Kate Galloway

    Article

    Wang et al Cell Syst. 2025 Mar 10, Volume 16, Issue

    Insert

    HRAS(G12V)-IRES-p53DD (HRAS, Trp53 Human, Mouse)
    

    Use

    Retroviral

    Tags

    Expression

    Mammalian

    Mutation

    G12V

    Promoter

    Available since

    March 13, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pCAGGS puro HA-hUSP27X

    Plasmid

    #225718

    Purpose

    Bacterial expression of USP27X with HA tag

    Depositor

    Francisco Bustos

    Article

    Koch et al Life Sci Alliance. 2024 Jan 5;7(3):e2023

    Insert

    USP27X (USP27X Human)
    

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Sept. 18, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pKLV-EF1a-mCherryCXCR4-W

    Plasmid

    #200102

    Purpose

    Lentiviral vector expressing mCherry fused with CXCR4 cDNA at the C-terminus

    Depositor

    Kosuke Yusa

    Article

    Aoki et al Blood. 2023 Nov 3:blood.2023020857. doi:

    Insert

    CXCR4 (CXCR4 Human)
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mutation

    silent mutations (c.180C>T, 438G>A, 495C>…

    Promoter

    Available since

    Jan. 17, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge