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pAVA4173 [CMV-TRNAU1AP(Δ184-278)-FLAG]

Plasmid

#254880

Purpose

Expresses CMV-driven C-terminal FLAG-tagged TRNAU1AP (Δ184-278)

Depositor

Andrei Alexandrov

Publication

Panah et al Nat Commun. 2026 Jun 11. doi: 10.1038/s4

Insert

Amino acid 184-278 truncated TRNAU1AP

Tags

FLAG

Expression

Mammalian

Promoter

CMV

Available since

June 4, 2026

Availability

Academic Institutions and Nonprofits only

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pAVA4176 [CMV-TRNAU1AP(Δ248-278)-FLAG]

Plasmid

#254881

Purpose

Expresses CMV-driven C-terminal FLAG-tagged TRNAU1AP (Δ248-278)

Depositor

Andrei Alexandrov

Publication

Panah et al Nat Commun. 2026 Jun 11. doi: 10.1038/s4

Insert

Amino acid 248-278 truncated TRNAU1AP

Tags

FLAG

Expression

Mammalian

Promoter

CMV

Available since

June 4, 2026

Availability

Academic Institutions and Nonprofits only

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LentiCRISPR-B_sgRNA_WDR61 (pAVA3321)

Plasmid

#254886

Purpose

Lentiviral vector expressing Cas9-P2A-Blasticidin and an sgRNA targeting WDR61

Depositor

Andrei Alexandrov

Publication

Panah et al Nat Commun. 2026 Jun 11. doi: 10.1038/s4

Insert

Sinlge sgRNA targeting WDR61

Use

CRISPR and Lentiviral

Promoter

U6

Available since

June 4, 2026

Availability

Academic Institutions and Nonprofits only

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pAVA3932 [TRNAU1AP minigene: exon3-intron3 including poison exon-exon4]

Plasmid

#254878

Purpose

Expresses EFS-driven TRNAU1AP minigene (exon3--poison exon-containing intron3-exon4)

Depositor

Andrei Alexandrov

Publication

Panah et al Nat Commun. 2026 Jun 11. doi: 10.1038/s4

Insert

TRNAU1AP minigene (exon3-poison exon-containing intron3-exon4)

Expression

Mammalian

Promoter

EFS

Available since

June 4, 2026

Availability

Academic Institutions and Nonprofits only

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pAVA3936 [PRPF39 minigene: exon8-intron8 including poison exon-exon9]

Plasmid

#254879

Purpose

Expresses EFS-driven PRPF39 minigene (exon8-poison exon-containing intron8-exon9)

Depositor

Andrei Alexandrov

Publication

Panah et al Nat Commun. 2026 Jun 11. doi: 10.1038/s4

Insert

PRPF39 minigene (exon8-poison exon-containing intton8-exon9)

Expression

Mammalian

Promoter

EFS

Available since

June 4, 2026

Availability

Academic Institutions and Nonprofits only

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EGFP_EHMT1_N300

Plasmid

#228814

Purpose

Expression of human EHMT1 N-terminal 300 aa only

Depositor

Shravanti Rampalli

Publication

Biligiri et al PLoS Biol. 2024 Nov 7;22(11):e3002871. d

Insert

EHMT1 (EHMT1 Human)

Tags

EGFP

Expression

Mammalian

Mutation

contains aa 1-300 only

Available since

Aug. 6, 2025

Availability

Academic Institutions and Nonprofits only

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EGFP_EHMT1_Ank+SET

Plasmid

#228815

Purpose

Expression of human EHMT1 with deletion of N-terminal 300 aa

Depositor

Shravanti Rampalli

Publication

Biligiri et al PLoS Biol. 2024 Nov 7;22(11):e3002871. d

Insert

EHMT1 (EHMT1 Human)

Tags

EGFP

Expression

Mammalian

Mutation

deletion of N-terminal 300 aa

Available since

Aug. 6, 2025

Availability

Academic Institutions and Nonprofits only

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EGFP_EHMT1B1-3

Plasmid

#228811

Purpose

Expression of mouse EHMT1 with deletion of aa 1185-1298

Depositor

Shravanti Rampalli

Publication

Biligiri et al PLoS Biol. 2024 Nov 7;22(11):e3002871. d

Insert

EHMT1 (Ehmt1 Mouse)

Tags

EGFP

Expression

Mammalian

Mutation

deletion of aa 1185-1298

Available since

March 10, 2025

Availability

Academic Institutions and Nonprofits only

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EGFP_EHMT1B1-4

Plasmid

#228812

Purpose

Expression of mouse EHMT1 with deletion of aa 809-1298

Depositor

Shravanti Rampalli

Publication

Biligiri et al PLoS Biol. 2024 Nov 7;22(11):e3002871. d

Insert

EHMT1 (Ehmt1 Mouse)

Tags

EGFP

Expression

Mammalian

Mutation

deletion of aa 809-1298, See Depositor Comments

Available since

March 10, 2025

Availability

Academic Institutions and Nonprofits only

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EGFP_EHMT1V09

Plasmid

#228813

Purpose

Expression of mouse EHMT1 with deletion of aa 1-31

Depositor

Shravanti Rampalli

Publication

Biligiri et al PLoS Biol. 2024 Nov 7;22(11):e3002871. d

Insert

EHMT1 (Ehmt1 Mouse)

Tags

EGFP

Expression

Mammalian

Mutation

deletion of aa 1-31

Available since

March 10, 2025

Availability

Academic Institutions and Nonprofits only

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pRDB_268

Plasmid

#231112

Purpose

SpG-Cas9

Depositor

John Doench, David Root

Publication

Kaplan et al Nat Genet. 2025 Nov;57(11):2920-2929. do

Insert

Cas9-SpG

Use

CRISPR and Lentiviral

Available since

Jan. 16, 2025

Availability

Academic Institutions and Nonprofits only

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CRaf-IRES-pErk reporter

Plasmid

#226170

Purpose

Used to transiently express Myc-tagged CRaf with the pErk reporter. Used in LABEL-seq MAPK activity assays.

Depositor

Dustin Maly

Publication

Simon et al Nat Methods. 2024 Nov;21(11):2094-2106.

Insert

Myc-CRaf-IRES-3xFlag-tdMCP-Erk2 (MAPK1, RAF1 Human)

Tags

3xFlag-tdMCP and Myc

Expression

Mammalian

Available since

Nov. 12, 2024

Availability

Academic Institutions and Nonprofits only

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pAMC042z21

Plasmid

#209747

Purpose

RepA(N99D) SC101 T0-BS7 proD SD8 sfGFP( V93AGGA Y151UAGA) ProK 5 tRNA operon tetA-orfL bla

Depositor

Ahmed Badran

Publication

Costello et al Nat Biotechnol. 2024 Sep 11. doi: 10.103

Insert

sfGFP - tRNA operon

Expression

Bacterial

Mutation

sfGFP - Valine 93 AGGA quadruplet codon, Tyrosine…

Available since

Oct. 18, 2024

Availability

Academic Institutions and Nonprofits only

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pRSETA-qmTQ2-glucose

Plasmid

#225252

Purpose

Bacterial expression of N-terminal His-tagged qmTQ2-glucose, a cyan fluorescent FLIM biosensor for detecting glucose

Depositor

Yasushi Okada

Publication

Zhong et al Cell Rep Methods. 2024 Nov 18;4(11):1009

Insert

qmTQ2-glucose

Tags

6XHis, T7 tag, XpressTM tag

Expression

Bacterial

Promoter

T7 promoter

Available since

Sept. 20, 2024

Availability

Academic Institutions and Nonprofits only

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Efg1-ΔDBD-Y115S/Y132S/Y139S

Plasmid

#216408

Purpose

Expresses 6xHis-tagged N- and C-terminal prion-like domains of Efg1 with Y115/Y132/Y139 to serine mutations

Depositor

Nicolas Fawzi

Publication

Wang et al Biophys J. 2024 Jun 4;123(11):1481-1493.

Insert

Efg1-ΔDBD-Y115S/Y132S/Y139S

Tags

6xHis-tags

Expression

Bacterial

Mutation

changed Tyrosine 115, 132 and 139 to Serine, and …

Available since

July 1, 2024

Availability

Academic Institutions and Nonprofits only

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pNoRBS

Plasmid

#218167

Purpose

pET31b plasmid with short insert lacking ribosome binding site to increase 2',3'-cNMP levels

Depositor

Emily Weinert

Publication

Chauhan et al ACS Cent Sci. 2022 Nov 23;8(11):1518-152

Insert

Short insert

Expression

Bacterial

Promoter

T7

Available since

June 3, 2024

Availability

Academic Institutions and Nonprofits only

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Efg1-ΔDBD-A51P/A56P/Q443P

Plasmid

#216402

Purpose

Expresses 6xHis-tagged N- and C-terminal prion-like domains of Efg1 with A51/A56/Q443 to proline mutations

Depositor

Nicolas Fawzi

Publication

Wang et al Biophys J. 2024 Jun 4;123(11):1481-1493.

Insert

Efg1-ΔDBD-A51P/A56P/Q443P

Tags

6xHis-tags

Expression

Bacterial

Mutation

changed Alanine 51, 56 and Glutamine 443 to Proli…

Available since

April 17, 2024

Availability

Academic Institutions and Nonprofits only

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Efg1-ΔDBD-A51P/A56P/Q70P/Q443P

Plasmid

#216403

Purpose

Expresses 6xHis-tagged N- and C-terminal prion-like domains of Efg1 with A51/A56/Q70P/Q443 to proline mutations

Depositor

Nicolas Fawzi

Publication

Wang et al Biophys J. 2024 Jun 4;123(11):1481-1493.

Insert

Efg1-ΔDBD-A51P/A56P/Q70P/Q443P

Tags

6xHis-tags

Expression

Bacterial

Mutation

changed Alanine 51, 56 and Glutamine 70, 443 to P…

Available since

April 17, 2024

Availability

Academic Institutions and Nonprofits only

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Efg1-ΔDBD-A51P/A56P/Q70P/N102P/Q443P

Plasmid

#216404

Purpose

Expresses 6xHis-tagged N- and C-terminal prion-like domains of Efg1 with A51/A56/Q70P/N102/Q443 to proline mutations

Depositor

Nicolas Fawzi

Publication

Wang et al Biophys J. 2024 Jun 4;123(11):1481-1493.

Insert

Efg1-ΔDBD-A51P/A56P/Q70P/N102P/Q443P

Tags

6xHis-tags

Expression

Bacterial

Mutation

changed Alanine 51, 56, Glutamine 70, 443 and Asp…

Available since

April 17, 2024

Availability

Academic Institutions and Nonprofits only

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Efg1-ΔDBD-Y4S/Y8S/Y16S

Plasmid

#216405

Purpose

Expresses 6xHis-tagged N- and C-terminal prion-like domains of Efg1 with Y4/Y8/Y16 to serine mutations

Depositor

Nicolas Fawzi

Publication

Wang et al Biophys J. 2024 Jun 4;123(11):1481-1493.

Insert

Efg1-ΔDBD-Y4S/Y8S/Y16S

Tags

6xHis-tags

Expression

Bacterial

Mutation

changed Tyrosine 4, 8 and 16 to Serine, and delet…

Available since

April 17, 2024

Availability

Academic Institutions and Nonprofits only

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