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p026 pCS2-EnR

Plasmid

#11028

Depositor

Ramesh Shivdasani

Article

Shoichet et al Proc Natl Acad Sci U S A. 2000 Apr 11. 9

Insert

engrailed (en Fly)

Use

Xenopus, mammalian, avian, and zebrafish

Mutation

Engrailed repressor domain (bases encoding amino …

Available Since

Jan. 5, 2006

Availability

Academic Institutions and Nonprofits only

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GAL-p300-501-999

Plasmid

#89100

Purpose

mammalian expression of human p300 amino acids 501-999 with N-terminal GAL4 DNA binding domain

Depositor

Elizabeth Wilson

Article

Askew et al J Biol Chem. 2010 Jul 9;285(28):21824-36

Insert

p300 (EP300 Human)

Tags

GAL4 DBD

Expression

Mammalian

Mutation

aa 501-999

Available Since

April 5, 2017

Availability

Academic Institutions and Nonprofits only

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ADAMTS5_pCSdest

Plasmid

#53819

Depositor

Roger Reeves

Article

Edie et al G3 (Bethesda). 2018 Jul 2;8(7):2215-2223

Insert

ADAMTS5 (ADAMTS5 Human)

Expression

Mammalian

Promoter

SP6 and CMV

Available Since

March 27, 2018

Availability

Industry, Academic Institutions, and Nonprofits

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pBabe-Puro-REDD1-HA

Plasmid

#133550

Purpose

Expresses HA tagged REDD1 in Mammalian cells

Depositor

James Brugarolas

Article

Vega-Rubin-de-Celis et al Biochemistry. 2010 Mar 23;49(11):2491-50

Insert

Regulated in development and DNA damage response 1 (REDD1) (DDIT4 Human)

Use

Retroviral

Tags

HA tag

Expression

Mammalian

Available Since

Oct. 30, 2019

Availability

Academic Institutions and Nonprofits only

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pQHA-USP11 WT puroR

Plasmid

#46749

Purpose

Retroviral vector that expresses wild type HA-tagged wild type human USP11

Depositor

Goedele Maertens, Gordon Peters

Article

Maertens et al EMBO J. 2010 Aug 4;29(15):2553-65. doi:

Insert

USP11 (USP11 Human)

Use

Lentiviral

Tags

HA

Expression

Mammalian

Promoter

CMV

Available Since

March 19, 2015

Availability

Academic Institutions and Nonprofits only

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3543 pMIG Bcl-XL mut8

Plasmid

#8792

Depositor

Stanley Korsmeyer

Article

Cheng et al Mol Cell 2001 Sep;8(3):705-11.

Insert

Bcl-XL (BCL2L1 Human)

Use

Retroviral

Expression

Mammalian

Mutation

G138A, R139L, I140N

Available Since

July 6, 2005

Availability

Academic Institutions and Nonprofits only
pAS_4xMma PylT_FLAG-AcKRS

Plasmid

#140021

Purpose

Plasmid with 4xMmaPylT cassette and MmaPylRS for amber suppression and incorporation of acetyl-lysine; for transient or stable piggyBac-mediated integration

Depositor

Simon Elsaesser

Article

Meineke et al Cell Rep Methods. 2023 Nov 20;3(11):1006

Insert

AcKRS-3 (pylS Methanosarcina mazei)

Tags

FLAG

Expression

Mammalian

Mutation

L301M, L305I, Y306F, L309A, C348F

Promoter

EF1

Available Since

May 20, 2020

Availability

Academic Institutions and Nonprofits only

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pET21-pelB-LaM-6

Plasmid

#172771

Purpose

Bacterial expression of anti-mCherry nanobody LaM-6, with pelB leader and C-term free cysteine and 6xHIS tag.

Depositor

Michael Rout

Article

Fridy et al Nat Methods. 2014 Dec;11(12):1253-60. do

Insert

LaM-6

Tags

6xHIS and pelB leader for periplasmic secretion

Expression

Bacterial

Promoter

T7

Available Since

Aug. 18, 2021

Availability

Academic Institutions and Nonprofits only

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pENTR_sfGFP-TurboID

Plasmid

#240226

Purpose

Gateway entry clone with TurboID tagged with superfolder GFP (contains stop codon)

Depositor

Justin Bosch, Norbert Perrimon

Article

Bosch et al bioRxiv [Preprint]. 2025 Jul 11:2025.07.

Insert

sfGFP-TurboID

Use

Gateway shuttle vector

Available Since

July 11, 2025

Availability

Academic Institutions and Nonprofits only

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pKM19

Plasmid

#123655

Purpose

Encodes firefly luciferase under the pIEx promoter for constitutive expression in insect (e.g. Drosophila, mosquito) cells

Depositor

Kevin Maringer

Article

Fredericks et al PLoS Negl Trop Dis. 2019 Nov 6;13(11):e0

Insert

Firefly luciferase

Use

Luciferase

Expression

Insect

Promoter

pIEx

Available Since

May 7, 2019

Availability

Academic Institutions and Nonprofits only

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pEGFP-MyosinVa-Ctail

Plasmid

#110169

Purpose

Dominant negative effect by competing with MyosinVa C-terminal tail interactions

Depositor

Jose Esteban

Article

Correia et al Nat Neurosci. 2008 Apr;11(4):457-66. doi

Insert

Myosin Va (Myo5a Mouse)

Tags

EGFP and Myc

Expression

Mammalian

Mutation

amino acids 1444-1853

Promoter

CMV

Available Since

May 29, 2018

Availability

Academic Institutions and Nonprofits only

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pQHA-USP11 CS puroR

Plasmid

#46750

Purpose

Retroviral vector that expresses catalytically inactive form of HA-tagged human USP11

Depositor

Goedele Maertens, Gordon Peters

Article

Maertens et al EMBO J. 2010 Aug 4;29(15):2553-65. doi:

Insert

USP11 (USP11 Human)

Use

Retroviral

Tags

HA

Expression

Mammalian

Mutation

C318S--catalytically inactive

Promoter

CMV

Available Since

March 19, 2015

Availability

Academic Institutions and Nonprofits only

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pAS_4xBstTyrT(CUA)_EcoTyrRS-FLAG

Plasmid

#140018

Purpose

Plasmid with 4xBstTyrT(CUA) cassette and E. coli TyrRS for amber suppression; for transient or stable piggyBac-mediated integration

Depositor

Simon Elsaesser

Article

Meineke et al Cell Rep Methods. 2023 Nov 20;3(11):1006

Insert

EcoTyrRS

Tags

FLAG

Expression

Mammalian

Mutation

Y37L, D182S, F183M, L186A

Promoter

EF1

Available Since

May 13, 2020

Availability

Academic Institutions and Nonprofits only

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pCMV-hARdelta120-472

Plasmid

#89088

Purpose

mammalian expression of human androgen receptor with deletion of 120-472 amino acid residues

Depositor

Elizabeth Wilson

Article

Bai et al Mol Cell Biol. 2008 Mar;28(6):1947-63. d

Insert

hAR-120-472 N-term deletion (AR Human)

Expression

Mammalian

Mutation

hAR-120-472

Promoter

CMV

Available Since

April 11, 2017

Availability

Academic Institutions and Nonprofits only

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pCDNA3-EMTB-TurboID-V5

Plasmid

#190736

Purpose

Mammalian expression of EMTB fusion to V5-tagged TurboID on microtubules (EMTB as the microtubule-targeting tag)

Depositor

Jeremy Baskin

Article

Cao et al ACS Chem Biol. 2022 Aug 11. doi: 10.1021

Insert

ensconsin (MAP7 Human)

Tags

Myc, TurboID, and V5

Expression

Mammalian

Mutation

Microtubule binding-domain of ensconsin (amino ac…

Promoter

CMV

Available Since

Dec. 8, 2022

Availability

Academic Institutions and Nonprofits only

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ESR1-luciferase

Plasmid

#113351

Purpose

evaluate FOXA1 DIV motif enhancer activity near 50 kb of ESR1 gene

Depositor

Ralf Jauch

Article

Wang et al Nucleic Acids Res. 2018 Jun 20;46(11):54

Insert

ESR1-enhancer (ESR1 Human)

Use

Luciferase

Available Since

Sept. 12, 2018

Availability

Academic Institutions and Nonprofits only

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HypaCas9

Plasmid

#138557

Purpose

Expresses human codon-optimized hyper-accurate SpCas9 and blasticidin resistance: EFS promoter-HypaCas9-NLS-FLAG-P2A-BSD

Depositor

Hyongbum Kim

Article

Kim et al Nat Biotechnol. 2020 Nov;38(11):1328-133

Insert

HypaCas9

Use

CRISPR and Lentiviral

Tags

BSD, FLAG, and NLS

Expression

Mammalian

Mutation

N692A, M694A, Q695A, H698A

Promoter

EFS

Available Since

June 9, 2020

Availability

Academic Institutions and Nonprofits only

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Cx30-msfGFP

Plasmid

#69019

Purpose

Expresses human Cx30 (GJB6 CDS) with a 7 amino acid linker on the C-terminus of the Connexin linking to monomerized superfolder Green Fluorescent Protein. Expression driven by CMV promoter.

Depositor

David Spray

Article

Stout RF et al J Biol Chem. 2015 Aug 11. pii: jbc.M115.

Insert

Cx30 (GJB6 Human)

Tags

V206K monomerized super folder GFP

Expression

Mammalian

Promoter

c

Available Since

Oct. 23, 2015

Availability

Academic Institutions and Nonprofits only

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MTK0_062

Plasmid

#123951

Purpose

Encodes the 2nd gen. Lenti Virus GFP dropout destination vector without insulator as a type 0 part to be used in the MTK system

Depositor

Hana El-Samad

Article

Fonseca et al ACS Synth Biol. 2019 Nov 15;8(11):2593-2

Insert

pHR DEST

Expression

Mammalian

Available Since

Dec. 13, 2019

Availability

Academic Institutions and Nonprofits only

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pGL4 mIL-17 2kb RORgamma-t mut

Plasmid

#20127

Depositor

Warren Strober

Article

Fuping et al 2008 Nov;9(11):1297-306. Epub 2008 Oct 1

Insert

mIL-17 (Il17a Mouse)

Use

Luciferase

Mutation

RORgamma-t binding site in 0.9kb of 2kb promoter …

Available Since

Jan. 16, 2009

Availability

Academic Institutions and Nonprofits only