We narrowed to 10,366 results for: ADA

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pCHC022 (∆phcA)

Plasmid

#226200

Purpose

Electroporation knockout plasmid (pK18sB) to delete the C. necator LysR-type transcriptional regulator PhcA.

Depositor

Christopher Johnson

Article

Calvey et al Metab Eng. 2023 Jan;75:78-90. doi: 10.10

Insert

Homology arms for PhcA

Use

Synthetic Biology

Available Since

Nov. 18, 2024

Availability

Industry, Academic Institutions, and Nonprofits

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pCHC004 (∆phaCAB)

Plasmid

#226198

Purpose

Electroporation knockout plasmid (pK18sB) to delete the C. necator phaCAB operon, to prevent accumulation of PHB.

Depositor

Christopher Johnson

Article

Calvey et al Metab Eng. 2023 Jan;75:78-90. doi: 10.10

Insert

Homology arms for phaCAB

Use

Synthetic Biology

Available Since

Nov. 15, 2024

Availability

Industry, Academic Institutions, and Nonprofits

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pCHC027 (∆pemK)

Plasmid

#226204

Purpose

Conjugation knockout plasmid (pK18msB) to delete the C. necator addiction system toxin PemK, to enable deletion of the pHG1 megaplasmid.

Depositor

Christopher Johnson

Article

Calvey et al Metab Eng. 2023 Jan;75:78-90. doi: 10.10

Insert

Upstream/downstream homology arms for addiction system toxinPemK.

Use

Synthetic Biology

Available Since

Nov. 14, 2024

Availability

Industry, Academic Institutions, and Nonprofits

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pAM94

Plasmid

#226686

Purpose

GGG-TitinV13PV15P-23-K-9-C-25-intein-CBD

Depositor

Andreas Martin

Article

Bard et al Cell. 2019 Apr 4;177(2):286-298.e15. doi

Insert

titin (TTN Human)

Tags

GyrA inteine- Chitin Binding domain and cyclin B

Expression

Bacterial

Promoter

T7

Available Since

Oct. 31, 2024

Availability

Academic Institutions and Nonprofits only

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pAM99

Plasmid

#226691

Purpose

TitinV15P-23-K-9-C-1

Depositor

Andreas Martin

Article

Bard et al Cell. 2019 Apr 4;177(2):286-298.e15. doi

Insert

titin (TTN Human)

Tags

GyrA inteine- Chitin Binding domain and cyclin B

Expression

Bacterial

Promoter

T7

Available Since

Oct. 31, 2024

Availability

Academic Institutions and Nonprofits only

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pAM97

Plasmid

#226689

Purpose

GGG-Titinv15P-23-K-9-K-9-K-8-C-25

Depositor

Andreas Martin

Article

Bard et al Cell. 2019 Apr 4;177(2):286-298.e15. doi

Insert

titin (TTN Human)

Tags

GyrA inteine- Chitin Binding domain and cyclin B

Expression

Bacterial

Promoter

T7

Available Since

Oct. 31, 2024

Availability

Academic Institutions and Nonprofits only

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pAM104

Plasmid

#226696

Purpose

GGG-TitinV15P-43-K-11-intein-CBD

Depositor

Andreas Martin

Article

Bard et al Cell. 2019 Apr 4;177(2):286-298.e15. doi

Insert

titin (TTN Human)

Tags

GyrA inteine- Chitin Binding domain and cyclin B

Expression

Bacterial

Promoter

T7

Available Since

Oct. 31, 2024

Availability

Academic Institutions and Nonprofits only

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pAM100

Plasmid

#226692

Purpose

TitinV15P-21-C-2-K-1

Depositor

Andreas Martin

Article

Bard et al Cell. 2019 Apr 4;177(2):286-298.e15. doi

Insert

titin (TTN Human)

Tags

GyrA inteine- Chitin Binding domain and cyclin B

Expression

Bacterial

Promoter

T7

Available Since

Oct. 31, 2024

Availability

Academic Institutions and Nonprofits only

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pAM98

Plasmid

#226690

Purpose

TitinV15P-23-K-9-C-15

Depositor

Andreas Martin

Article

Bard et al Cell. 2019 Apr 4;177(2):286-298.e15. doi

Insert

titin (TTN Human)

Tags

GyrA inteine- Chitin Binding domain and cyclin B

Expression

Bacterial

Promoter

T7

Available Since

Oct. 31, 2024

Availability

Academic Institutions and Nonprofits only

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pAM105

Plasmid

#226697

Purpose

GGG-TitinV15P-23-K-19-K-11-intein-CBD

Depositor

Andreas Martin

Article

Bard et al Cell. 2019 Apr 4;177(2):286-298.e15. doi

Insert

titin (TTN Human)

Tags

GyrA inteine- Chitin Binding domain and cyclin B

Expression

Bacterial

Promoter

T7

Available Since

Oct. 31, 2024

Availability

Academic Institutions and Nonprofits only

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pAM95

Plasmid

#226687

Purpose

GGG-TitinV15P-43-K-8-C-25-intein-CBD

Depositor

Andreas Martin

Article

Bard et al Cell. 2019 Apr 4;177(2):286-298.e15. doi

Insert

titin (TTN Human)

Tags

GyrA inteine- Chitin Binding domain and cyclin B

Expression

Bacterial

Promoter

T7

Available Since

Oct. 31, 2024

Availability

Academic Institutions and Nonprofits only

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pHDR_NPM2-T2A-mGreenLantern-PuroTK

Plasmid

#222910

Purpose

Homology directed repair template for knocking in mGreenLantern reporter to NPM2.

Depositor

George Church

Article

Pierson Smela et al bioRxiv 2022.07.11.499564

Insert

mGreenLantern

Use

CRISPR

Available Since

July 22, 2024

Availability

Academic Institutions and Nonprofits only

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pHDR_FIGLA-T2A-mGreenLantern-PuroTK

Plasmid

#222905

Purpose

Homology directed repair template for knocking in mGreenLantern reporter to FIGLA.

Depositor

George Church

Article

Pierson Smela et al bioRxiv 2022.07.11.499564

Insert

mGreenLantern

Use

CRISPR

Available Since

July 22, 2024

Availability

Academic Institutions and Nonprofits only

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pHDR2_TFAP2C-T2A-mGreenLantern-Hygro

Plasmid

#222915

Purpose

Homology directed repair template for knocking in mGreenLantern reporter to TFAP2C.

Depositor

George Church

Article

Pierson Smela et al bioRxiv 2022.07.11.499564

Insert

mGreenLantern

Use

CRISPR

Available Since

July 22, 2024

Availability

Academic Institutions and Nonprofits only

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pPB-cT3G-cERP2-ZGLP1

Plasmid

#222926

Purpose

PiggyBac transposon plasmid for doxycycline inducible expression of ZGLP1

Depositor

George Church

Article

Pierson Smela et al bioRxiv 2022.07.11.499564

Insert

ZGLP1 (ZGLP1 Human)

Expression

Mammalian

Available Since

July 19, 2024

Availability

Academic Institutions and Nonprofits only

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pPB-cT3G-cERH-SOHLH1

Plasmid

#222922

Purpose

PiggyBac transposon plasmid for doxycycline inducible expression of SOHLH1

Depositor

George Church

Article

Pierson Smela et al bioRxiv 2022.07.11.499564

Insert

SOHLH1 (SOHLH1 Human)

Expression

Mammalian

Available Since

July 19, 2024

Availability

Academic Institutions and Nonprofits only

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opt.a_pSTC0-zeo

Plasmid

#202347

Purpose

Design opt.a (greedy optimization without distance constraints) in modified pSTC0 vector in which kan resistance cassette is replaced with zeo resistance cassette

Depositor

Nicholas Gauthier

Article

Fram et al Nat Commun. 2024 Jun 20;15(1):5141. doi:

Insert

opt.a

Available Since

June 26, 2024

Availability

Academic Institutions and Nonprofits only

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opt.b_pSTC0-zeo

Plasmid

#202348

Purpose

Design opt.b (parallel tempering optimization without distance constraints) in modified pSTC0 vector in which kan resistance cassette is replaced with zeo resistance cassette

Depositor

Nicholas Gauthier

Article

Fram et al Nat Commun. 2024 Jun 20;15(1):5141. doi:

Insert

opt.b

Available Since

June 26, 2024

Availability

Academic Institutions and Nonprofits only

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neg.ctrl_pSTC0-zeo

Plasmid

#202350

Purpose

Control neg. ctrl (negative control S70A) in modified pSTC0 vector in which kan resistance cassette is replaced with zeo resistance cassette

Depositor

Nicholas Gauthier

Article

Fram et al Nat Commun. 2024 Jun 20;15(1):5141. doi:

Insert

neg. ctrl

Available Since

June 26, 2024

Availability

Academic Institutions and Nonprofits only

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Q54A

Plasmid

#217912

Purpose

A plasmid encoding the hydrogel-forming protein PXP with the Q54A coil mutation

Depositor

Cole DeForest

Article

Bennett et al Adv Sci (Weinh). 2024 Mar 13:e2301708. d

Insert

PXP Q54A

Tags

6x His (N and C term)

Expression

Bacterial

Mutation

Glutamine at position 55 and 267 mutated to Alani…

Promoter

T5

Available Since

June 24, 2024

Availability

Academic Institutions and Nonprofits only

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