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T7-VEE-IRES-Puro

Plasmid

#58970

Purpose

noninfectious (non-packaging), self-replicating Venezuelan equine encephalitis (VEE) virus RNA replicon

Depositor

Steven Dowdy

Publication

Yoshioka et al Cell Stem Cell. 2013 Aug 1;13(2):246-54.

Type

Empty backbone

Use

Venezuelan equine encephalitis (vee) virus rna re…

Expression

Mammalian

Promoter

26S subgenomic promoter

Available since

Oct. 2, 2014

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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T7-VEE-IRES-Puro

Plasmid

#58970

Purpose

noninfectious (non-packaging), self-replicating Venezuelan equine encephalitis (VEE) virus RNA replicon

Depositor

Steven Dowdy

Publication

Yoshioka et al Cell Stem Cell. 2013 Aug 1;13(2):246-54.

Type

Empty backbone

Use

Venezuelan equine encephalitis (vee) virus rna re…

Expression

Mammalian

Promoter

26S subgenomic promoter

Available since

Oct. 2, 2014

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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pL-CRISPR.SFFV.GFP

Plasmid

#57827

Purpose

Lentiviral CRISPR-Cas9 delivery for SpCas9 and sgRNA. Coexpresses eGFP via P2A cleavage site. SFFV Promoter driven

Depositor

Benjamin Ebert

Publication

Heckl et al Nat Biotechnol. 2014 Jun 22. doi: 10.103

Inserts

SpCas9
Sp sgRNA scaffold
SFFV
P2A-eGFP

Use

CRISPR and Lentiviral

Tags

FLAG

Available since

Aug. 20, 2014

Availability

Academic Institutions and Nonprofits only

Cited by

20 Articles

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pL-CRISPR.SFFV.GFP

Plasmid

#57827

Purpose

Lentiviral CRISPR-Cas9 delivery for SpCas9 and sgRNA. Coexpresses eGFP via P2A cleavage site. SFFV Promoter driven

Depositor

Benjamin Ebert

Publication

Heckl et al Nat Biotechnol. 2014 Jun 22. doi: 10.103

Inserts

SpCas9
Sp sgRNA scaffold
SFFV
P2A-eGFP

Use

CRISPR and Lentiviral

Tags

FLAG

Available since

Aug. 20, 2014

Availability

Academic Institutions and Nonprofits only

Cited by

20 Articles

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AAVS1-CAG-hrGFP

Plasmid

#52344

Purpose

AAVS1 donor vector for constitutive gene expression

Depositor

Su-Chun Zhang

Publication

Qian et al Stem Cells. 2014 Feb 4. doi: 10.1002/ste

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

hrGFP

Expression

Mammalian

Promoter

CAG

Available since

July 21, 2014

Availability

Academic Institutions and Nonprofits only

Cited by

30 Articles

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AAVS1-CAG-hrGFP

Plasmid

#52344

Purpose

AAVS1 donor vector for constitutive gene expression

Depositor

Su-Chun Zhang

Publication

Qian et al Stem Cells. 2014 Feb 4. doi: 10.1002/ste

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

hrGFP

Expression

Mammalian

Promoter

CAG

Available since

July 21, 2014

Availability

Academic Institutions and Nonprofits only

Cited by

30 Articles

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pET15b-AavLEA1

Plasmid

#53093

Purpose

AavLEA1 expression (in BL21 DE3 E. coli strain)

Depositor

Claude Férec

Publication

Tripathi et al PLoS One. 2014 Aug 13;9(8):e104970. doi:

Insert

AavLEA1

Tags

6xHis

Expression

Bacterial

Promoter

T7

Available since

June 26, 2014

Availability

Academic Institutions and Nonprofits only

Cited by

4 Articles

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pET15b-AavLEA1

Plasmid

#53093

Purpose

AavLEA1 expression (in BL21 DE3 E. coli strain)

Depositor

Claude Férec

Publication

Tripathi et al PLoS One. 2014 Aug 13;9(8):e104970. doi:

Insert

AavLEA1

Tags

6xHis

Expression

Bacterial

Promoter

T7

Available since

June 26, 2014

Availability

Academic Institutions and Nonprofits only

Cited by

4 Articles

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pCMV-Bx

Plasmid

#51552

Purpose

Expresses Bxb1 integrase in mammalian cells.

Depositor

Michele Calos

Publication

Zhu et al Nucleic Acids Res. 2013 Dec 4.

Insert

Bxb1 integrase

Expression

Mammalian

Promoter

CMV

Available since

March 3, 2014

Availability

Academic Institutions and Nonprofits only

Cited by

3 Articles

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pCMV-Bx

Plasmid

#51552

Purpose

Expresses Bxb1 integrase in mammalian cells.

Depositor

Michele Calos

Publication

Zhu et al Nucleic Acids Res. 2013 Dec 4.

Insert

Bxb1 integrase

Expression

Mammalian

Promoter

CMV

Available since

March 3, 2014

Availability

Academic Institutions and Nonprofits only

Cited by

3 Articles

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pST1374-N-NLS-flag-linker-Cas9-D10A

Plasmid

#51130

Purpose

Cas9 nickase optimized for nuclear import.

Depositor

Xingxu Huang

Publication

Shen et al Nat Methods. 2014 Mar 2. doi: 10.1038/nm

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

Cas9 nickase

Use

CRISPR

Tags

Flag and NLS

Expression

Mammalian

Mutation

D10A (nickase mutant)

Promoter

CMV

Available since

Feb. 11, 2014

Availability

Academic Institutions and Nonprofits only

Cited by

9 Articles

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pST1374-N-NLS-flag-linker-Cas9-D10A

Plasmid

#51130

Purpose

Cas9 nickase optimized for nuclear import.

Depositor

Xingxu Huang

Publication

Shen et al Nat Methods. 2014 Mar 2. doi: 10.1038/nm

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

Cas9 nickase

Use

CRISPR

Tags

Flag and NLS

Expression

Mammalian

Mutation

D10A (nickase mutant)

Promoter

CMV

Available since

Feb. 11, 2014

Availability

Academic Institutions and Nonprofits only

Cited by

9 Articles

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pcDNA3-Drp1K38A

Plasmid

#45161

Depositor

Alexander van der Bliek, Richard Youle

Publication

Smirnova et al J Cell Biol. 1998 Oct 19;143(2):351-8.

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

Drp1 (DNM1L Human)

Tags

HA (AYPYDVPDY)

Expression

Mammalian

Mutation

K38A

Promoter

CMV

Available since

May 22, 2013

Availability

Academic Institutions and Nonprofits only

Cited by

8 Articles

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pcDNA3-Drp1K38A

Plasmid

#45161

Depositor

Alexander van der Bliek, Richard Youle

Publication

Smirnova et al J Cell Biol. 1998 Oct 19;143(2):351-8.

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

Drp1 (DNM1L Human)

Tags

HA (AYPYDVPDY)

Expression

Mammalian

Mutation

K38A

Promoter

CMV

Available since

May 22, 2013

Availability

Academic Institutions and Nonprofits only

Cited by

8 Articles

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Lenti-Hb9-GFP

Plasmid

#37080

Purpose

3rd generation lentiviral transfer plasmid for Hb9 GFP

Depositor

Fred Gage

Publication

Marchetto et al Cell Stem Cell. 2008 Dec 4;3(6):649-57.

Insert

Hb9 GFP

Use

Lentiviral

Promoter

Hb9

Available since

Oct. 15, 2012

Availability

Academic Institutions and Nonprofits only

Cited by

6 Articles

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Lenti-Hb9-GFP

Plasmid

#37080

Purpose

3rd generation lentiviral transfer plasmid for Hb9 GFP

Depositor

Fred Gage

Publication

Marchetto et al Cell Stem Cell. 2008 Dec 4;3(6):649-57.

Insert

Hb9 GFP

Use

Lentiviral

Promoter

Hb9

Available since

Oct. 15, 2012

Availability

Academic Institutions and Nonprofits only

Cited by

6 Articles

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beta2-spectrin-HA

Plasmid

#31070

Depositor

Vann Bennett

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

beta2-spectrin (SPTBN1 Human)

Tags

HA

Expression

Mammalian

Available since

March 15, 2012

Availability

Academic Institutions and Nonprofits only

Cited by

8 Articles

Enlarge
beta2-spectrin-HA

Plasmid

#31070

Depositor

Vann Bennett

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

beta2-spectrin (SPTBN1 Human)

Tags

HA

Expression

Mammalian

Available since

March 15, 2012

Availability

Academic Institutions and Nonprofits only

Cited by

8 Articles

Enlarge
pCFJ350 - MCS(ttTi5605, II)

Plasmid

#34866

Depositor

Erik Jorgensen

Publication

Frokjaer-Jensen et al Nat Methods. 2012 Jan 30;9(2):117-8. doi

Insert

ttTi5605 targeting

Use

Worm targeting

Available since

Feb. 1, 2012

Availability

Academic Institutions and Nonprofits only

Cited by

15 Articles

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pCFJ350 - MCS(ttTi5605, II)

Plasmid

#34866

Depositor

Erik Jorgensen

Publication

Frokjaer-Jensen et al Nat Methods. 2012 Jan 30;9(2):117-8. doi

Insert

ttTi5605 targeting

Use

Worm targeting

Available since

Feb. 1, 2012

Availability

Academic Institutions and Nonprofits only

Cited by

15 Articles

Enlarge