We narrowed to 30,799 results for: Ide

1. pRK7-HA-S6K1-F5A

   Plasmid

   #8986

   Depositor

   John Blenis

   Article

   Schalm et al Curr Biol. 2002 Apr 16. 12(8):632-9.

   Insert

   S6K1 (Rps6kb1 Rat)
   

   Tags

   HA

   Expression

   Mammalian

   Mutation

   F5A

   Available Since

   April 4, 2006

   Availability

   Academic Institutions and Nonprofits only

2. pML104-KanMx4

   Plasmid

   #83476

   Purpose

   Expresses Cas9 and contains guide RNA expression cassette with BclI-SwaI cloning sites for guide sequence cloning; Contains KanMx4 marker for yeast transformation.

   Depositor

   Patricia Wittkopp

   Insert

   KanMX4
   

   Expression

   Yeast

   Available Since

   Oct. 5, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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3. OmpA3-PNGaseF-TEV-His6

   Plasmid

   #114274

   Purpose

   Amidase removing almost all N-linked oligosaccharides from glycoproteins. This PNGase F construct can have its hexa-his removed.

   Depositor

   Martin Caffrey

   Insert

   Peptide: N-glycosidase F
   

   Tags

   OmpA3 leader sequence (for periplasmic localisati…

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   Aug. 13, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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4. pMDC32-HPB

   Plasmid

   #32078

   Depositor

   Fumi Katagiri

   Article

   Qi et al Plant J. 2009 Mar;57(5):932-44. Epub 200

   Type

   Empty backbone

   Use

   Gateway destination binary vector

   Tags

   HA-Prescission-Biotin

   Promoter

   35S promoter

   Available Since

   Dec. 6, 2011

   Availability

   Academic Institutions and Nonprofits only

   

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5. pX458-Ef1a-dCas9-VPR-H2B-GFP (CRISPRa)

   Plasmid

   #175571

   Purpose

   Inactivated Cas9 (dCas9) fusion to VPR for the purpose of targeted activation

   Depositor

   Joseph Brzezinski

   Type

   Empty backbone

   Use

   CRISPR

   Available Since

   Oct. 7, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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6. pAG426-GAL-Kar2-Beta-Amyloid

   Plasmid

   #181707

   Purpose

   Galactose inducible expression of Kar2-Beta-Amyloid

   Depositor

   Alejandro Chavez

   Insert

   Kar2-Beta-Amyloid
   

   Expression

   Yeast

   Available Since

   April 24, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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7. pX458-Ef1a-dCas9-VPR-H2B-mCherry (CRISPRa)

   Plasmid

   #175572

   Purpose

   Inactivated Cas9 (dCas9) fusion to VPR for the purpose of targeted activation

   Depositor

   Joseph Brzezinski

   Type

   Empty backbone

   Use

   CRISPR

   Available Since

   Oct. 7, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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8. pML104-HygMx4

   Plasmid

   #83475

   Purpose

   Expresses Cas9 and contains guide RNA expression cassette with BclI-SwaI cloning sites for guide sequence cloning; Contains HphMx4 marker for yeast transformation.

   Depositor

   Patricia Wittkopp

   Insert

   HphMX4
   

   Expression

   Yeast

   Available Since

   Oct. 12, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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9. pSBBi-Pur GFP-TM

   Plasmid

   #139185

   Purpose

   SB-transposon with constitutive bi-directional promoter. One side contains transmembrane-anchored GFP; the other side contains the puromycin resistance gene.

   Depositor

   Neal Devaraj

   Insert

   GFP-TM
   

   Use

   Transposon

   Expression

   Mammalian

   Mutation

   fused to membrane anchor

   Promoter

   EF1α

   Available Since

   March 27, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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10. pET22b-ELP[V]60

    Plasmid

    #219406

    Purpose

    (VPGVG)60

    Depositor

    Danielle Mai

    Article

    Wirtz et al Biomacromolecules. 2024 Jul 9. doi: 10.1

    Insert

    ELP[V]60
    

    Tags

    6xHis

    Expression

    Bacterial

    Available Since

    July 10, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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11. Ub-GyrA

    Plasmid

    #128737

    Purpose

    Expresses Ub fused to GyrA intein at the C-terminal

    Depositor

    Sharon Rozovsky

    Article

    Liu et al Chembiochem. 2019 Jul 2. doi: 10.1002/cb

    Insert

    UBC
    

    Tags

    Mxe GyrA

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Sept. 18, 2019

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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12. pML104-NatMx3

    Plasmid

    #83477

    Purpose

    Expresses Cas9 and contains guide RNA expression cassette with BclI-SwaI cloning sites for guide sequence cloning; Contains NatMX3 marker for yeast transformation.

    Depositor

    Patricia Wittkopp

    Insert

    NatMx3
    

    Expression

    Yeast

    Available Since

    Oct. 5, 2016

    Availability

    Academic Institutions and Nonprofits only

13. pBAC-RT

    Plasmid

    #16418

    Depositor

    Craig Strathdee

    Article

    Stavropoulos et al Genomics. 2001 Feb 15. 72(1):99-104.

    Insert

    E. coli rpsL gene; tetA(C) gene
    

    Use

    Bacmid

    Available Since

    June 2, 2008

    Availability

    Academic Institutions and Nonprofits only

14. m-sGFP1-10::NLG1

    Plasmid

    #44967

    Depositor

    Joshua Sanes

    Article

    Yamagata et al Front Mol Neurosci. 2012;5:18. Epub 2012

    Insert

    m-sGFP1-10::Neuroligin1
    

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    July 10, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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15. m-sGFP11::NXN

    Plasmid

    #44968

    Depositor

    Joshua Sanes

    Article

    Yamagata et al Front Mol Neurosci. 2012;5:18. Epub 2012

    Insert

    pCMV m-sGFP11::NXN
    

    Expression

    Mammalian

    Available Since

    June 11, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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16. pCT02

    Plasmid

    #212152

    Purpose

    Plasmid expressing 6x His tagged FAST-PETase under inducible promoter PT7 control.

    Depositor

    Kang Zhou

    Insert

    FAST-PETase
    

    Tags

    6x His

    Expression

    Bacterial

    Mutation

    S121E, D186H, R224Q, N233K, R280A

    Promoter

    PT7

    Available Since

    March 7, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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17. tTA-IRES-Neo

    Plasmid

    #16541

    Depositor

    Bert Vogelstein

    Article

    Yu et al Proc Natl Acad Sci U S A. 1999 Dec 7. 96

    Insert

    tTA
    

    Expression

    Mammalian

    Available Since

    April 16, 2015

    Availability

    Academic Institutions and Nonprofits only

18. pCI-6.20

    Plasmid

    #41002

    Depositor

    James Inglese

    Article

    Cheng et al Nat Methods. 2012 Oct;9(10):937. doi: 10

    Insert

    GSG-P2A-RLuc
    

    Use

    Luciferase

    Expression

    Mammalian

    Promoter

    SV40

    Available Since

    Nov. 13, 2012

    Availability

    Academic Institutions and Nonprofits only

    

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19. pHelper_Ec1_V1_gentR

    Plasmid

    #205291

    Purpose

    Helper plasmid for ORBIT in E. coli. Expresses RecT for oligo recombineering and Bxb-1 for pInt incorporation. Has sacB for counter selection.

    Depositor

    Scott Saunders

    Article

    Saunders et al bioRxiv 2023

    Insert

    CspRecT / Bxb-1
    

    Use

    Synthetic Biology

    Available Since

    Aug. 23, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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20. pHelper_Ec1_V1_ampR

    Plasmid

    #205292

    Purpose

    Helper plasmid for ORBIT in E. coli. Expresses RecT for oligo recombineering and Bxb-1 for pInt incorporation. Has sacB for counter selection.

    Depositor

    Scott Saunders

    Article

    Saunders et al bioRxiv 2023

    Insert

    CspRecT / Bxb-1
    

    Use

    Synthetic Biology

    Available Since

    Aug. 16, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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