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CMVTO-SEAP-26Sfur-B2AD-hcvs-KKMP

Plasmid

#179638

Purpose

HCVP-inducible single TM SEAP RELEASE construct

Depositor

Xiaojing Gao

Article

Vlahos et al Nat Commun. 2022 Feb 17;13(1):912. doi:

Insert

SEAP RELEASE

Expression

Mammalian

Available Since

March 23, 2022

Availability

Academic Institutions and Nonprofits only

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PB-CMV-TO-rapTEV-teHCV-hcTVMV-tvDiTEV-Neo

Plasmid

#116095

Purpose

Expresses a combination of proteases and their reporter

Depositor

Michael Elowitz

Article

Gao et al Science. 2018 Sep 21;361(6408):1252-1258

Insert

rapTEV-teHCV-hcTVMV-tvDiTEV-Neo

Expression

Mammalian

Available Since

Oct. 15, 2018

Availability

Academic Institutions and Nonprofits only

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p6346 MSCV-CMV-Flag-HA-Brd4-1-444

Plasmid

#32886

Depositor

Peter Howley

Article

Rahman et al Mol Cell Biol. 2011 Jul . 31(13):2641-52

Insert

Brd4 (BRD4 Human)

Use

Retroviral

Tags

Flag and HA

Expression

Mammalian

Promoter

CMV

Available Since

Jan. 4, 2012

Availability

Academic Institutions and Nonprofits only

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pLV-CMV-EGFP-PolB-PAMmut-Hygro

Plasmid

#176086

Purpose

EGFP fused to the N-terminus of a POLB containing a mutation in the PAM site used by POLBKOg1 & a hygromycin resistance cassette

Depositor

Robert Sobol

Article

Koczor et al Cell Rep. 2021 Nov 2;37(5):109917. doi:

Insert

PolB (POLB Human)

Use

Lentiviral

Tags

EGFP

Expression

Mammalian

Mutation

Mutation in the PAM site used by PolBKOg1

Promoter

CMV

Available Since

Jan. 26, 2022

Availability

Academic Institutions and Nonprofits only

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CMVTO-IL12-26Sfur-B2AD-tvmvs-KKMP

Plasmid

#179646

Purpose

TVMVP-inducible IL-12 RELEASE construct

Depositor

Xiaojing Gao

Article

Vlahos et al Nat Commun. 2022 Feb 17;13(1):912. doi:

Insert

IL12 A/B

Expression

Mammalian

Available Since

March 23, 2022

Availability

Academic Institutions and Nonprofits only

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Polymerase expression - pCMV-Phi29(+exo) (LM2759)

Plasmid

#208964

Purpose

Unfused Phi29 DNA polymerase (+exo), expressed from CMV or T7 promoters.

Depositor

Benjamin Kleinstiver

Article

Ferreira da Silva et al Nat Biotechnol. 2024 Jul 22. doi: 10.103

Insert

Phi29(+exo)-BPNLS

Use

CRISPR; In vitro transcription; t7 promoter

Tags

BPNLS

Expression

Mammalian

Promoter

CMV and T7

Available Since

Nov. 27, 2023

Availability

Academic Institutions and Nonprofits only

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pJEP228-Lenti-CMV-GFP-GluN2B-WPRE-pA

Plasmid

#84277

Purpose

pLenti vector backbone designed to express GFP tagged GluN2B from a CMV promoter.

Depositor

Jonathan Ploski

Article

Holehonnur et al Mol Brain. 2015 Feb 24;8:12. doi: 10.118

Insert

GluN2B

Use

Lentiviral

Tags

GFP

Promoter

CMV

Available Since

July 7, 2022

Availability

Academic Institutions and Nonprofits only

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pJEP227-Lenti-CMV-GFP-GluN2A-WPRE-pA

Plasmid

#84276

Purpose

pLenti vector backbone designed to express GFP tagged GluN2A from a CMV promoter.

Depositor

Jonathan Ploski

Article

Holehonnur et al Mol Brain. 2015 Feb 24;8:12. doi: 10.118

Insert

GluN2A

Use

Lentiviral

Tags

GFP

Promoter

CMV

Available Since

March 17, 2022

Availability

Academic Institutions and Nonprofits only

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pAAV-CMV-FRT-myrSNAP-2A-H2BeGFP-WPRE

Plasmid

#102986

Purpose

AAV expressing membrane-targeted SNAP protein and nuclear-targeted eGFP after FLP-mediated recombination

Depositor

Marco Tripodi

Article

Ciabatti et al Cell. 2017 Jul 1. pii: S0092-8674(17)306

Inserts

membrane-targeted SNAP protein after FLP-mediated recombination
Nuclear-targeted eGFP after FLP-mediated recombination

Use

AAV; Adeno associated viral vector

Available Since

Dec. 8, 2017

Availability

Academic Institutions and Nonprofits only

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pBabe-TetCMV-puro-mVenus-PA-Rac1-C450M

Plasmid

#22034

Depositor

Klaus Hahn

Article

Wu et al Nature. 2009 Sep 3. 461(7260):104-8.

Insert

mVenus-PA-Rac1-C450M (RAC1 Human, Avena sativa (oat))

Use

Retroviral

Expression

Mammalian

Mutation

The LOV domain contains the mutation C450M.Rac1 s…

Available Since

Sept. 29, 2009

Availability

Academic Institutions and Nonprofits only
pTS94 pHAGE2 CMVtetO2 GFP-22xGS-3C-MCS

Plasmid

#199347

Purpose

Lentiviral transfer plasmid for doxycycline inducible expression of a N-terminally GFP-3C-tagged protein in mammalian cells.

Depositor

Rebecca Voorhees

Article

Stevens et al Nat Protoc. 2024 Jan;19(1):127-158. doi:

Type

Empty backbone

Use

Lentiviral

Tags

EGFP-3C

Expression

Mammalian

Mutation

WT

Available Since

April 21, 2023

Availability

Academic Institutions and Nonprofits only

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pMMK ENTR 1 CMV mAG beta-catenin

Plasmid

#206258

Purpose

ENTR Vector 1 for MultiSite Gateway assembly and production of recombinant baculovirus using MultiMate assembly. Encodes mAG Ctnnb1 under the control of CMV promoter.

Depositor

Imre Berger

Article

Aulicino et al Nucleic Acids Res. 2022 Jul 22;50(13):77

Insert

mAG Ctnnb1 (Ctnnb1 Mouse)

Use

Multimate/gateway entr 1

Tags

monomeric Azami Green

Expression

Mammalian

Available Since

Sept. 27, 2023

Availability

Academic Institutions and Nonprofits only

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pLenti CMV Puro DEST (w118-1) MXRA7

Plasmid

#107500

Purpose

Expresses MXRA7, puromycin resistant

Depositor

Alejandro Gutierrez

Article

Aries et al J Exp Med. 2018 Nov 7. pii: jem.20180570

Insert

MXRA7 (codon optimized) (MXRA7 Human)

Use

Lentiviral

Expression

Mammalian

Mutation

Codon optimized sequence

Promoter

CMV

Available Since

Nov. 7, 2018

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1_pCMV-nCas-PmCDA1 pH1-gRNA(HPRT)

Plasmid

#79619

Purpose

Expresses nCas9-PmCDA1 and gRNA(HPRT) in mammalian cells

Depositor

Akihiko Kondo

Article

Nishida et al Science. 2016 Aug 4. pii: aaf8729.

Insert

SpCas9

Tags

3xFlag-PmCDA1

Expression

Mammalian

Mutation

D10A for nickase Cas9

Promoter

pCMV

Available Since

July 27, 2016

Availability

Academic Institutions and Nonprofits only

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pLU-CMV-PL3 Flag-humanTRAF3-WT (1114)

Plasmid

#66922

Purpose

Lentiviral Expression of TRAF3

Depositor

Michael Karin

Insert

TRAF3 (TRAF3 Human)

Use

Lentiviral

Tags

FLAG

Expression

Mammalian

Promoter

CMV

Available Since

Aug. 7, 2017

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1_pCMV-dCas-PmCDA1 pH1-gRNA(HPRT)

Plasmid

#79621

Purpose

Expresses dCas9-PmCDA1 and gRNA(HPRT) in mammalian cells

Depositor

Akihiko Kondo

Article

Nishida et al Science. 2016 Aug 4. pii: aaf8729.

Insert

SpCas9

Tags

PmCDA1

Expression

Mammalian

Mutation

D10A and H840A for nuclease deficient Cas9

Promoter

pCMV

Available Since

Sept. 2, 2016

Availability

Academic Institutions and Nonprofits only

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pRP(Exp)-CMV> ORF_924bp (Azgp1):T2A:dTomato:IRES:Puro

Plasmid

#110830

Purpose

Expresses zinc alpha-2 glycoprotein (ZAG) and dTomato (dT) reporter where both sequences are separated by a T2A self-cleaving peptide sequence as a result, the dT is not fused with the ZAG protein.

Depositor

Ande Satyanarayana

Article

Elattar et al FASEB J. 2018 Mar 23:fj201701465RR. doi:

Insert

AZGP1 (Azgp1 Mouse)

Use

Non-viral gene expression vector

Tags

T2A and dTomato

Promoter

CMV

Available Since

June 27, 2018

Availability

Academic Institutions and Nonprofits only

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pHTN HaloTag CMV-neo/ERGIC2-variant

Plasmid

#51477

Purpose

Expresses ERGIC2-variant in mammalian cells, HaloTag for cellular localization

Depositor

Simon Kwok

Article

Kwok et al DNA Cell Biol. 2013 Dec 4.

Insert

ERGIC2 (ERGIC2 Human)

Tags

HaloTag

Expression

Mammalian

Mutation

4 base deletion at codon #189

Available Since

April 15, 2014

Availability

Academic Institutions and Nonprofits only
pBabe-TetCMV-puro-mVenus-PA-Rac1-C450A

Plasmid

#22023

Depositor

Klaus Hahn

Article

Wu et al Nature. 2009 Sep 3. 461(7260):104-8.

Insert

mVenus-PA-Rac1-C450A (RAC1 Human, Avena sativa (oat))

Use

Retroviral

Expression

Mammalian

Mutation

The LOV domain contains the mutation C450A.Rac1 s…

Available Since

Sept. 29, 2009

Availability

Academic Institutions and Nonprofits only
pCMV5-Flag-EPLIN alpha delta LIM

Plasmid

#40933

Depositor

Elizabeth Luna, Scott Maul

Article

Song et al Mol Biol Cell. 2002 Apr;13(4):1408-16.

Insert

EPLIN alpha (LIMA1 Human)

Tags

Flag

Expression

Mammalian

Mutation

LIM domain deleted- amino acids 221-295

Available Since

Dec. 3, 2012

Availability

Academic Institutions and Nonprofits only