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pET11a-hRanGAP1

Plasmid

#50941

Purpose

expression of full length untagged human RanGAP1 in E.coli

Depositor

Frauke Melchior

Publication

Werner et al Mol Cell. 2012 May 11;46(3):287-98. doi:

Insert

RanGTPase activating protein 1 (RANGAP1 Human)

Expression

Bacterial

Promoter

T7

Available since

May 30, 2014

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pDONR223-IPMK

Plasmid

#23666

Depositor

William Hahn, David Root

Publication

Johannessen et al Nature. 2010 Dec 16;468(7326):968-72. do

Insert

IPMK (IPMK Human)

Use

Gateway Cloning

Available since

July 30, 2010

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article
pAAV-DIO-TVA-V5-RG

Plasmid

#119743

Purpose

Expresses TVA, V5, and RG in a cre dependent manner.

Depositor

Marie Carlén

Publication

Ahrlund-Richter et al Nat Neurosci. 2019 Apr;22(4):657-668. do

Insert

TVA, V5, Rabies Glycoprotein

Use

AAV and Cre/Lox

Tags

V5

Available since

April 26, 2019

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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pcDNA-Ran-Q69L-mRFP1-polyA

Plasmid

#104560

Purpose

Express Ran Q69L mutant in mammalian cells (CMV promoter). Also suitable for in vitro transcription (T7 promoter) for injecting mRNA into oocytes.

Depositor

Yi Zhang

Publication

Inoue et al Nat Struct Mol Biol. 2014 Jul;21(7):609-

Insert

Ran (Ran Mouse)

Tags

mRFP1

Expression

Mammalian

Mutation

Q69L

Available since

Dec. 21, 2017

Availability

Academic Institutions and Nonprofits only

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pERB219

Plasmid

#61501

Purpose

Const. Halotag-GFP-PACT + mCherry-DHFR

Depositor

David Chenoweth, Michael Lampson

Publication

Ballister et al Nat Commun. 2014 Nov 17;5:5475. doi: 10.

Inserts

HaloTag-GFP-PACT
mCherry-DHFR

Tags

HaloTag and mCherry

Expression

Mammalian

Available since

May 18, 2015

Availability

Academic Institutions and Nonprofits only

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pSF279

Plasmid

#167674

Purpose

Intermediate vector for five gRNAs using GoldenGate, contains ccdB and attL1-L2 sites for Gateway

Depositor

Niko Geldner

Publication

Ursache et al Plant Methods. 2021 Oct 30;17(1):111. do

Insert

attL1-ccdB-attL2

Use

CRISPR

Available since

July 20, 2021

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pFU-GW-sMYH6-mNeon-TNNT2-mScarlet

Plasmid

#170712

Purpose

dual fluorescent reporter for MYH6 (short) and TNNT2 promoters

Depositor

Paul Burridge

Publication

Romero-Tejeda et al J Mol Cell Cardiol. 2023 Jul 6:S0022-282

Inserts

mNeonGreen
mScarlet

Use

Lentiviral

Expression

Mammalian

Promoter

TNNT2 and aMHC

Available since

July 12, 2023

Availability

Academic Institutions and Nonprofits only

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pCAG_Xph20-GCaMP7f

Plasmid

#135539

Purpose

Encodes a specific PSD-95 binder (Xph20) fused to GCaMP7f

Depositor

Matthieu Sainlos

Publication

Rimbault et al Elife. 2024 Jan 3;13:e69620. doi: 10.755

Insert

Xph20

Tags

GCaMP7f

Expression

Mammalian

Promoter

CAG

Available since

April 9, 2021

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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Zebrabow-GateDest

Plasmid

#118221

Purpose

For cloning promoter into a Zebrabow vector with Gateway reaction. Only compatible with single site Gateway. Not compatible with Tol2-kit.

Depositor

Albert Pan, Alex Schier

Publication

Pan et al Development. 2013 Jul;140(13):2835-46. d

Insert

Brainbow 1.0 L - ccdB survival cassette

Use

Zebrafish expression

Available since

Nov. 7, 2018

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pMA-SpCas9-g5

Plasmid

#80788

Purpose

BsaI-based cloning of SpCas9 gRNA guide sequence, position 5/15/25 in the array

Depositor

Yonglun Luo

Publication

Vad-Nielsen et al Cell Mol Life Sci. 2016 May 13.

Insert

CRISPR gRNA expression cassette (for SpCas9)

Use

CRISPR

Tags

na

Expression

Mammalian

Promoter

U6

Available since

Sept. 13, 2016

Availability

Academic Institutions and Nonprofits only

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pLH-sgRNA1-boxB-MS2

Plasmid

#75396

Purpose

sgRNA1-boxB-MS2

Depositor

Thoru Pederson

Publication

Ma et al Nat Biotechnol. 2016 Apr 18. doi: 10.103

Insert

sgRNA1-boxB-MS2

Use

CRISPR and Lentiviral

Tags

no

Expression

Mammalian

Promoter

U6

Available since

May 25, 2016

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pUBC_HA_2xPP7-VP64

Plasmid

#68418

Purpose

Transient expression of two tandem copies of the non-aggregating delta-FG mutant, fused to the VP64 transcription activator, in mammalian cells, under a UBC promoter

Depositor

John Rinn

Publication

Shechner et al Nat Methods. 2015 Jul;12(7):664-70. doi:

Insert

PP7(deltaFG)

Use

Synthetic Biology

Tags

HA and VP64

Expression

Mammalian

Mutation

PP7: [delta]67_74 (non-aggregating)

Promoter

human UbC

Available since

Oct. 5, 2015

Availability

Academic Institutions and Nonprofits only

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pJFRC173-20XUAS-IVS-Dre::PEST

Plasmid

#32146

Purpose

Expresses Dre recombinase under control of 20XUAS sites in Drosophila

Depositor

Gerald Rubin

Publication

Nern et al Proc Natl Acad Sci U S A. 2011 Aug 9.

Insert

Dre::PEST

Expression

Insect

Promoter

Hsp70

Available since

Sept. 23, 2011

Availability

Academic Institutions and Nonprofits only

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pCMV-hATG7CS

Plasmid

#87868

Purpose

Express a active-site mutant human Atg7/Apg7 C572S in mammalian cells

Depositor

Eiki Kominami, Isei Tanida

Publication

Tanida et al J Biol Chem. 2001 Jan 19;276(3):1701-6.

Insert

ATG7 C572S mutant (ATG7 Human)

Expression

Mammalian

Mutation

C572S mutant

Promoter

CMV

Available since

Aug. 5, 2019

Availability

Academic Institutions and Nonprofits only

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pTwist-SARS-CoV Δ18

Plasmid

#169465

Purpose

Encodes SARS-CoV Spike Protein lacking 18 C-terminal amino acids for pseudovirus production

Depositor

Alejandro Balazs

Publication

Garcia-Beltran et al Cell. 2021 Mar 12. pii: S0092-8674(21)00

Insert

SARS-CoV Spike truncated to remove 18 amino acids (S )

Expression

Mammalian

Available since

June 21, 2021

Availability

Academic Institutions and Nonprofits only

Cited by

6 Articles

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pSF143-wArchon1-KGC-EGFP-ER2

Plasmid

#108429

Purpose

Codon-optimized Archon1 fluorescent voltage reporter in C.elegans expression plasmid

Depositor

Edward Boyden

Publication

Piatkevich et al Nat Chem Biol. 2018 Feb 26. pii: 10.1038

Insert

wArchon1-KGC-EGFP-ER2

Expression

Worm

Promoter

flp-18

Available since

April 10, 2018

Availability

Academic Institutions and Nonprofits only

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pET15b LFn-Fla

Plasmid

#84871

Purpose

Bacterial expression of LFn-Fla fusion

Depositor

Russell Vance

Publication

Rauch et al J Exp Med. 2016 May 2;213(5):657-65. doi

Inserts

LFn
Fla

Tags

His

Expression

Bacterial

Promoter

T7

Available since

Nov. 14, 2016

Availability

Academic Institutions and Nonprofits only

Cited by

4 Articles
pJJH1295

Plasmid

#36914

Depositor

Jürgen Heinisch

Publication

Bakota et al Mol Genet Genomics. 2012 Apr;287(4):313-

Insert

3x mCherry

Expression

Bacterial, Mammalian, and Y…

Available since

Aug. 15, 2012

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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pT2/CMV-Luc/PGK-SB100X

Plasmid

#192869

Purpose

Carries luciferase under CMV promoter flanked with IR/DR elements. Also carries SB100X under PGK promoter outside of the IR/DR elements.

Depositor

Maria Castro, David Largaespada

Publication

Calinescu et al J Vis Exp. 2015 Feb 22;(96). doi: 10.379

Inserts

luc
SB100X

Expression

Mammalian

Available since

Jan. 9, 2023

Availability

Academic Institutions and Nonprofits only

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STAGR_gRNAScaffold_mU6

Plasmid

#102844

Purpose

Can be used as PCR template for a STAgR reaction

Depositor

Stefan Stricker

Publication

Breunig et al PLoS One. 2018 Apr 27;13(4):e0196015. do

Insert

STAgR Insert gRNAScaffold_mU6

Use

Pcr template for stagr inserts

Promoter

mU6

Available since

Feb. 6, 2019

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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