We narrowed to 14,429 results for: nts

1. pBABE-zeo small T 110

   Plasmid

   #9063

   Depositor

   Bob Weinberg

   Article

   Hahn et al Mol Cell Biol 2002 Apr;22(7):2111-23.

   Insert

   SV40 small T Antigen 110 (SV40gp7 simian virus)
   

   Use

   Retroviral

   Tags

   Expression

   Mammalian

   Mutation

   Contains only the N-termial 110 amino acids

   Promoter

   Available since

   Sept. 23, 2005

   Availability

   Academic Institutions and Nonprofits only

2. pQE30-roGFP1-iE

   Plasmid

   #83312

   Purpose

   Inducible expression of redox sensitive GFP in bacteria

   Depositor

   S. James Remington

   Article

   Lohman et al Biochemistry. 2008 Aug 19;47(33):8678-88

   Insert

   roGFP1-iE
   

   Use

   Tags

   6xHIS

   Expression

   Bacterial

   Mutation

   ro1(C48S/S147C/Q204C) plus X insertion, iE betwe…

   Promoter

   T5

   Available since

   Oct. 11, 2016

   Availability

   Academic Institutions and Nonprofits only

3. pLLX8

   Plasmid

   #79838

   Purpose

   Contains yeast counter selectable marker, CYH2, and ampicillin resistance cassette, used for making a DNA capture vector

   Depositor

   Cammie Lesser, Stephen Lory

   Article

   Wolfgang et al Proc Natl Acad Sci U S A. 2003 Jul 8;100

   Type

   Empty backbone

   Use

   Contains yeast counter selection gene, cyh2 and b…

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Sept. 11, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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4. pTH1-SaSrtA-KA

   Plasmid

   #64981

   Purpose

   Expresses the E105K/E108A mutant of Staphylococcus aureus sortase A in E. coli

   Depositor

   Teruyuki Nagamune

   Article

   Hirakawa et al Biotechnol Bioeng. 2012 Dec;109(12):2955

   Insert

   sortase A
   

   Use

   Tags

   His6 tag, Mxe GyrA intein, and chitin-binding pro…

   Expression

   Bacterial

   Mutation

   deleted amino acids 1-59, E105K, E108A

   Promoter

   Available since

   June 5, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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5. pFUPI

   Plasmid

   #80279

   Purpose

   A bicistronic lentiviral vector used to stably express cDNAs.

   Depositor

   Jeremy Luban

   Article

   Neagu et al J Clin Invest. 2009 Oct;119(10):3035-47.

   Insert

   Puro resistance cassette, IRES, cloning site
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   July 22, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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6. pAT15002-ABEmax

   Plasmid

   #163006

   Purpose

   plasmid expressing ABEmax

   Depositor

   Ervin Welker

   Article

   Talas et al Nat Commun. 2021 Nov 3;12(1):6353. doi:

   Insert

   ABEmax
   

   Use

   CRISPR

   Tags

   SV40 NLS

   Expression

   Mammalian

   Mutation

   Promoter

   EF1-a

   Available since

   Sept. 21, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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7. pAT15064-eCBE

   Plasmid

   #163009

   Purpose

   plasmid expressing eCBE

   Depositor

   Ervin Welker

   Article

   Talas et al Nat Commun. 2021 Nov 3;12(1):6353. doi:

   Insert

   eCBE
   

   Use

   CRISPR

   Tags

   3xFLAG and SV40 NLS

   Expression

   Mammalian

   Mutation

   Promoter

   EF1-a

   Available since

   Sept. 21, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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8. pEPOR2KN0040

   Plasmid

   #117607

   Purpose

   Level2 MoClo construct, containing level1 plant expression cassettes for SaCas9(pEPOR1CB0015) and sgRNA_NbPDS(pEPOR1CB0058)

   Depositor

   Nicola Patron

   Article

   Raitskin et al PLoS One. 2019 Feb 27;14(2):e0211598. do

   Insert

   [35S:SaCas9(pEPOR1CB0015) ] +[AtU6-26:sgRNA]
   

   Use

   Tags

   Expression

   Plant

   Mutation

   Promoter

   Available since

   Nov. 16, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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9. pYes2-AtPCS1

   Plasmid

   #49766

   Purpose

   Expresses Arabidopsis phytochelatin synthase (AtPCS1) in Saccharomyces Cerevisiae

   Depositor

   Julian Schroeder

   Article

   Clemens et al EMBO J. 1999 Jun 15;18(12):3325-33.

   Insert

   AtPCS1 (PCS1 Mustard Weed)
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   GAL1

   Available since

   Jan. 2, 2014

   Availability

   Academic Institutions and Nonprofits only

10. CBM9-N

    Plasmid

    #171710

    Purpose

    Expresses SARS-CoV-2 Nucleocapsid protein epitope

    Depositor

    Francis Nano

    Article

    McGuire et al Microb Cell Fact. 2022 Feb 5;21(1):21. d

    Insert

    SARS-CoV-2 Nucleocapsid (N )
    

    Use

    Tags

    CBM-9

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    July 19, 2021

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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11. CBM9-(PT4)P

    Plasmid

    #171708

    Purpose

    Carries CBM9 fusion tag

    Depositor

    Francis Nano

    Article

    McGuire et al Microb Cell Fact. 2022 Feb 5;21(1):21. d

    Insert

    Cellulose Bindind Protein 9
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    July 19, 2021

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

12. pRU205

    Plasmid

    #167685

    Purpose

    Final destination vector for single Gateway LR reaction

    Depositor

    Niko Geldner

    Article

    Ursache et al Plant Methods. 2021 Oct 30;17(1):111. do

    Insert

    PcUBQ4-2::asCpf1
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available since

    July 20, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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13. pRU321

    Plasmid

    #167681

    Purpose

    Final destination vector for single Gateway LR reaction

    Depositor

    Niko Geldner

    Article

    Ursache et al Plant Methods. 2021 Oct 30;17(1):111. do

    Insert

    PcUB4-2::asCas9
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available since

    July 20, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pRU320

    Plasmid

    #167682

    Purpose

    Final destination vector for single Gateway LR reaction

    Depositor

    Niko Geldner

    Article

    Ursache et al Plant Methods. 2021 Oct 30;17(1):111. do

    Insert

    PcUBQ4-2::asCas9
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available since

    July 20, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. PI(LM)-Rac1(WT)dc1.FLARE

    Plasmid

    #87590

    Purpose

    PI(LM): Photo-inhibitable/lit mutant I510E/I539E dc1.FLARE: built based on GTPase FLARE dual chain sensor

    Depositor

    Klaus Hahn

    Article

    Dagliyan et al Science. 2016 Dec 16;354(6318):1441-1444

    Inserts

    LOV2
    PBD
    Rac1
    

    Use

    Tags

    YPet and dTurq

    Expression

    Bacterial, Insect, and Mamm…

    Mutation

    Promoter

    CMV

    Available since

    July 10, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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16. pET:CCCT:GFP

    Plasmid

    #60738

    Purpose

    A modified pET28 driving expression of GFP, biobrick part Bba_E0040. No LacO site downstream of PT7. T7 promoter has base pairs 'CCCT' between -11 and -8.

    Depositor

    Matthew Bennett

    Article

    Shis et al Proc Natl Acad Sci U S A. 2013 Mar 26;11

    Insert

    GFP
    

    Use

    Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Dec. 18, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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17. pDG279

    Plasmid

    #85219

    Purpose

    mammalian expression of ubiquitin-K48R-EGFP fusion

    Depositor

    Douglas Gray

    Article

    Tsirigotis et al J Biol Chem. 2001 Dec 7;276(49):46073-8.

    Insert

    ubiquitin C (UBC Human)
    

    Use

    Tags

    6xHIS and EGFP

    Expression

    Mammalian

    Mutation

    K48R

    Promoter

    human ubiquitin C

    Available since

    Nov. 15, 2016

    Availability

    Academic Institutions and Nonprofits only

18. pEE386

    Plasmid

    #149284

    Purpose

    T-DNA encoding TRV2 with gRNA targeting NbAG

    Depositor

    Daniel Voytas

    Article

    Ellison et al Nat Plants. 2020 Jun 1. pii: 10.1038/s41

    Insert

    p35S:TRV2_NbAGsgRNA1:tNos
    

    Use

    Tags

    Expression

    Plant

    Mutation

    Promoter

    35S

    Available since

    July 20, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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19. pEE388

    Plasmid

    #149286

    Purpose

    T-DNA encoding TRV2 with mFT augmented gRNA targeting NbAG

    Depositor

    Daniel Voytas

    Article

    Ellison et al Nat Plants. 2020 Jun 1. pii: 10.1038/s41

    Insert

    p35S:TRV2_NbAGsgRNA1_mFT:tNos
    

    Use

    Tags

    Expression

    Plant

    Mutation

    Promoter

    35S

    Available since

    July 20, 2020

    Availability

    Academic Institutions and Nonprofits only

20. pEE385

    Plasmid

    #149289

    Purpose

    T-DNA encoding TRV2 with Truncated FT augmented gRNA targeting NbAG

    Depositor

    Daniel Voytas

    Article

    Ellison et al Nat Plants. 2020 Jun 1. pii: 10.1038/s41

    Insert

    p35S:TRV2_NbAGsgRNA2_TrunFT:tNos
    

    Use

    Tags

    Expression

    Plant

    Mutation

    Promoter

    35S

    Available since

    July 20, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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