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pDJSVT87

Plasmid

#109302

Purpose

Express periplasmic, outer membrane, or secreted proteins in E. coli - OmpA signal and His6 tag

Depositor

Daniel Slade

Article

Casasanta et al Methods Mol Biol. 2019;1960:123-138. doi

Type

Empty backbone

Tags

OmpA 1-27, 6His

Expression

Bacterial

Promoter

T7

Available Since

May 25, 2018

Availability

Academic Institutions and Nonprofits only

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pFLAG/HA-MOV10

Plasmid

#10976

Depositor

Thomas Tuschl

Article

Meister et al Curr Biol. 2005 Dec 6;15(23):2149-55. do

Insert

MOV10 (MOV10 Human)

Tags

FLAG and HA

Expression

Mammalian

Available Since

Feb. 13, 2006

Availability

Academic Institutions and Nonprofits only

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pSFFV_CloGFP(1-10)

Plasmid

#157994

Purpose

Lentiviral vector of constitutive expression of CloGFP(1-10)

Depositor

Bo Huang

Article

Zhou et al PLoS One. 2020 Nov 23;15(11):e0242592. d

Insert

CloGFP(1-10)

Use

Lentiviral

Expression

Mammalian

Mutation

G32Q, F46L, K101R, S202L

Promoter

SFFV

Available Since

Oct. 27, 2020

Availability

Academic Institutions and Nonprofits only

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pOPINM

Plasmid

#26044

Depositor

Ray Owens

Article

Berrow et al Nucleic Acids Res. 2007 . 35(6):e45.

Insert

His-MBP-3C-lacZ

Tags

MAHHHHHHSSG_MBP_LEVLFQGP

Expression

Bacterial, Insect, and Mamm…

Available Since

June 30, 2011

Availability

Academic Institutions and Nonprofits only

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pMLS328

Plasmid

#73717

Purpose

C. elegans germline CRE expression vector

Depositor

Erik Jorgensen

Article

Schwartz et al Genetics. 2016 Feb 2. pii: genetics.115.

Insert

2xNLS-CRE

Use

Cre/Lox

Expression

Worm

Promoter

Peft-3

Available Since

March 25, 2016

Availability

Academic Institutions and Nonprofits only

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PAP-delta10-cHIS

Plasmid

#21716

Depositor

Andrew Bohm

Article

Balbo et al Biochemistry. 2005 May 31. 44(21):7777-8

Insert

polyA polymerase (PAP1 Budding Yeast)

Tags

HIS

Expression

Bacterial

Mutation

30 amino acids off the c terminus

Available Since

Aug. 22, 2009

Availability

Academic Institutions and Nonprofits only
pAV-U6+27-Tornado-BroccoliSamSensor#1

Plasmid

#124363

Purpose

Overexpression of circular RNA (Broccoli-based SAM sensor)

Depositor

Samie Jaffrey

Article

Litke et al Nat Biotechnol. 2019 Apr 8. pii: 10.1038

Insert

Tornado-BroccoliSamSensor#1

Expression

Mammalian

Promoter

U6+27

Available Since

June 7, 2019

Availability

Academic Institutions and Nonprofits only

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pDiGi

Plasmid

#59323

Purpose

Inducible expression of dsRed and GFP in bacteria

Depositor

Sophie Helaine, David Holden

Article

Helaine et al Proc Natl Acad Sci U S A. 2010 Feb 23;10

Inserts

EGFP
dsRed

Expression

Bacterial

Promoter

Pbad and Plac

Available Since

Oct. 24, 2014

Availability

Academic Institutions and Nonprofits only

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pBAD-D2

Plasmid

#37470

Depositor

Amy Palmer, Roger Tsien

Article

Palmer et al Chem Biol. 2006 May;13(5):521-30.

Insert

D2 second generation cameleon (calcium sensor)

Expression

Bacterial

Promoter

Ara

Available Since

Oct. 11, 2012

Availability

Academic Institutions and Nonprofits only
pOPINE

Plasmid

#26043

Depositor

Ray Owens

Article

Berrow et al Nucleic Acids Res. 2007 . 35(6):e45.

Insert

His-lacZ-KHHHHHH

Tags

KHHHHHH and MAHHHHHHSSG

Expression

Bacterial, Insect, and Mamm…

Available Since

May 17, 2011

Availability

Academic Institutions and Nonprofits only

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pTHSSd_8

Plasmid

#59948

Purpose

PT7 GFP reporter

Depositor

Christopher Voigt

Article

Segall-Shapiro et al Mol Syst Biol. 2014 Jul 30;10(7):742.

Insert

sfGFP

Expression

Bacterial

Mutation

N/A

Promoter

PT7

Available Since

Oct. 15, 2014

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-TRPM5

Plasmid

#85189

Purpose

Expresses full-length mouse TRPM5 in mammalian cells

Depositor

Craig Montell

Article

Hofmann et al Curr Biol. 2003 Jul 1;13(13):1153-8.

Insert

TRPM5 (Trpm5 )

Expression

Mammalian

Promoter

CMV

Available Since

June 22, 2017

Availability

Academic Institutions and Nonprofits only

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p236

Plasmid

#186182

Purpose

Shotgun Metagenomic Spike-in

Depositor

Karsten Zengler

Article

Zaramela et al mSystems. 2022 Dec 20;7(6):e0044722. doi

Insert

synDNA 236

Use

Synthetic Biology; Cloning

Available Since

Sept. 26, 2022

Availability

Academic Institutions and Nonprofits only

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AAV-6P-SEW-YC3.6

Plasmid

#31379

Depositor

Fritjof Helmchen

Article

Minderer et al J Physiol. 2011 Nov 14.

Insert

Yellow Cameleon 3.60

Use

AAV

Tags

Venus and eCFP

Available Since

Nov. 29, 2011

Availability

Academic Institutions and Nonprofits only

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pENTR-SYNZIP17

Plasmid

#80671

Purpose

Encodes the synthetic coiled coil peptide SYNZIP17

Depositor

Amy Keating

Article

Thompson et al ACS Synth Biol. 2012 Apr 20;1(4):118-29.

Insert

SYNZIP17

Use

Entry vector

Available Since

Nov. 23, 2018

Availability

Academic Institutions and Nonprofits only

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pC1-HyPer-C199S

Plasmid

#42213

Purpose

Peroxide-insensitive version of HyPer to be used as a pH-control. Can be applied as a pH sensor.

Depositor

Vsevolod Belousov

Article

Belousov et al Nat Methods. 2006 Apr;3(4):281-6.

Insert

HYPER C199S

Expression

Mammalian

Promoter

CMV

Available Since

Dec. 6, 2013

Availability

Academic Institutions and Nonprofits only

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p126

Plasmid

#186176

Purpose

Shotgun Metagenomic Spike-in

Depositor

Karsten Zengler

Article

Zaramela et al mSystems. 2022 Dec 20;7(6):e0044722. doi

Insert

synDNA 126

Use

Synthetic Biology; Cloning

Available Since

Sept. 26, 2022

Availability

Academic Institutions and Nonprofits only

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JB85

Plasmid

#128909

Purpose

mammalian expression, proximity labelling, mitochondrial relocalisation

Depositor

Sean Munro

Article

Gillingham et al Elife. 2019 Jul 11;8. pii: 45916. doi: 1

Insert

Rab9ASN-BirA*-HA-MAO (RAB9A Human)

Expression

Mammalian

Available Since

Aug. 14, 2019

Availability

Academic Institutions and Nonprofits only

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pDONR207-Citrine-NLS

Plasmid

#44586

Purpose

Gateway entry vector for Citrine fluorescent protein with a nuclear localization signal

Depositor

Yutaka Kodama

Article

Ogasawara et al Plant Cell Environ. 2013 Feb 20. doi: 10

Insert

Citrine-NLS

Tags

NLS (Nuclear Localization Signal)

Available Since

Sept. 6, 2016

Availability

Academic Institutions and Nonprofits only

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pJBM4

Plasmid

#225291

Purpose

E. coli-Campylobacter shuttle vector; TetR

Depositor

Robert Moore

Article

McDonald et al mBio. 2024 Jul 29:e0110124. doi: 10.1128

Insert

Tet(O) and its putative promoter

Expression

Bacterial

Available Since

Oct. 7, 2024

Availability

Academic Institutions and Nonprofits only

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