We narrowed to 15,981 results for: LEA

1. pY017 (pcDNA3.1-hPcCpf1)

   Plasmid

   #69989

   Purpose

   Expresses humanized PcCpf1

   Depositor

   Feng Zhang

   Article

   Zetsche et al Cell. 2015 Sep 23. pii: S0092-8674(15)01

   Insert

   hPcCpf1
   

   Use

   CRISPR

   Tags

   NLS & 3xHA

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Oct. 8, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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2. pET41a iGluh

   Plasmid

   #119830

   Purpose

   Production of recombinant iGluSnFR E25A high affinity variant, a single-wavelength glutamate indicator, in E. coli

   Depositor

   Katalin Torok

   Article

   Helassa et al Proc Natl Acad Sci U S A. 2018 May 7. pi

   Insert

   iGluh
   

   Tags

   GST

   Expression

   Bacterial

   Mutation

   E25A

   Promoter

   T7

   Available Since

   Aug. 22, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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3. pET28a_Pro-IL-36β

   Plasmid

   #214344

   Purpose

   Protein overexpression in bacteria

   Depositor

   Jonathan Kagan

   Article

   Devant et al Nature. 2023 Dec;624(7991):451-459. doi:

   Insert

   IL36B (IL36B Human)
   

   Tags

   His-TEV and Myc

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   Feb. 21, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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4. pET21-pelB-LaG-17

   Plasmid

   #172747

   Purpose

   Bacterial expression of HIS-tagged anti-GFP nanobody LaG-17; HIS tag preceded by HRV 3C/PreScission cleavage site.

   Depositor

   Michael Rout

   Article

   Fridy et al Nat Methods. 2014 Dec;11(12):1253-60. do

   Insert

   LaG-17
   

   Tags

   6xHIS and pelB leader for periplasmic secretion

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   Aug. 20, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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5. pBT305_pBS-U-attPx3-FRT5-pSV40-Neo-pA-PL-FRT5

   Plasmid

   #52539

   Purpose

   Expression of FRT5-flanked Neo; homologous recombination targeting vector cloning

   Depositor

   Liqun Luo

   Article

   Tasic et al Proc Natl Acad Sci U S A. 2011 May 10;10

   Inserts

   PhiC31o attPx3 (70 bp each)
   Neo
   

   Expression

   Mammalian

   Available Since

   June 16, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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6. pGEMT-Map7-1333-end

   Plasmid

   #46070

   Depositor

   Edgar Gomes

   Article

   Metzger et al Nature. 2012 Mar 18;484(7392):120-4. doi

   Insert

   Map7-1333-end (Map7 Mouse)
   

   Expression

   Bacterial

   Available Since

   July 12, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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7. ALFY-FYVE 1G

   Plasmid

   #197045

   Purpose

   Expression plasmid for the FYVE domain from ALFY for recombinant expression in E. coli.

   Depositor

   Michael Ragusa

   Article

   Reinhart et al Traffic. 2021 Jan;22(1-2):23-37. doi: 10

   Insert

   ALFY (WDFY3 Human)
   

   Tags

   6xHis-GST-TEV

   Expression

   Bacterial

   Available Since

   Dec. 7, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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8. pCMV-FKBP-mTagBFP2-SOScat

   Plasmid

   #178866

   Purpose

   Mammalian expression of SOS1(Rem-Cdc25) fused to FKBP-mTagBFP2

   Depositor

   Shinya Tsukiji

   Article

   Yoshikawa et al J Am Chem Soc. 2021 May 5;143(17):6434-6

   Insert

   FKBP-mTagBFP2-SOS(Rem-Cdc25) (SOS1 Human)
   

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Jan. 10, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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9. pACEC3GH (Apr)

   Plasmid

   #110085

   Purpose

   Replicative, acetamide-based expression vector containing a C-terminal 3C cleavage site and a GPF-fusion with a His10-tag. Suitable for high-level expression levels amenable for large scale purification of proteins.

   Depositor

   Markus Seeger

   Article

   Arnold et al Sci Rep. 2018 Jun 22;8(1):9539. doi: 10.

   Type

   Empty backbone

   Tags

   3C cleavage site - GFP - His10 Tag

   Expression

   Bacterial

   Available Since

   June 5, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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10. pMIG-Flag-RIPK1-wt

    Plasmid

    #102677

    Purpose

    retroviral expression plasmid for RIPK1-wt

    Depositor

    Hasam Habelhah

    Article

    Zhang et al Mol Cell Biol. 2015 Oct;35(19):3324-38.

    Insert

    RIPK1 (RIPK1 Human)
    

    Use

    Retroviral

    Tags

    FLAG and IRES-GFP

    Expression

    Mammalian

    Promoter

    unknown

    Available Since

    Dec. 11, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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11. pAAV-hSyn-mCherry.3xFLAG-WPRE

    Plasmid

    #127861

    Purpose

    pAAV plasmid expressing an mCherry.3xFLAG fusion protein under the hSyn promoter

    Depositor

    Florian Freudenberg

    Article

    Candemir et al Eur Neuropsychopharmacol. 2016 Apr;26(4)

    Insert

    mCherry
    

    Use

    AAV

    Tags

    3xFLAG

    Promoter

    hSyn

    Available Since

    Aug. 2, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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12. pInsert-PhoCl-RER

    Plasmid

    #219676

    Purpose

    Caging at cytosolic C-ter of POI: mScarletCD4-PhoCl-RER in pTREtight2

    Depositor

    Helge Ewers

    Article

    Kashyap et al Nat Methods. 2024 Mar 8. doi: 10.1038/s4

    Insert

    CD4mScarlet-PhoCl-RER
    

    Tags

    mScarlet and PhoCl

    Expression

    Mammalian

    Promoter

    TRE

    Available Since

    June 14, 2024

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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13. pTY153 EGFP-T2A-ORF6 SARS-CoV-2

    Plasmid

    #204975

    Purpose

    Co-express EGFP and SARS-CoV-2 ORF6

    Depositor

    Tim Mitchison

    Article

    Yoo et al Proc Natl Acad Sci U S A. 2024 Jan 23;12

    Insert

    Open Reading Frame 6 (ORF6 SARS-CoV-2)
    

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    March 21, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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14. pAc5.1B-lambdaN-HA-DmPAN3-W707AG711E-dsRNAres_Y

    Plasmid

    #148087

    Purpose

    Insect Expression of DmPAN3-W707AG711E-dsRNAres

    Depositor

    Elisa Izaurralde

    Article

    Jonas et al Nat Struct Mol Biol. 2014 Jul;21(7):599-

    Insert

    DmPAN3-W707AG711E-dsRNAres (CG11486 Fly)
    

    Expression

    Insect

    Available Since

    March 16, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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15. pTY152 EGFP-T2A-ORF6 SARS-CoV-1

    Plasmid

    #204974

    Purpose

    Co-express EGFP and SARS-CoV-1 ORF6

    Depositor

    Tim Mitchison

    Article

    Yoo et al Proc Natl Acad Sci U S A. 2024 Jan 23;12

    Insert

    Open Reading Frame 6 (ORF6 SARS-CoV-1)
    

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    March 21, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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16. pCMV E2F1 1-284

    Plasmid

    #24216

    Purpose

    Mammalian expression of human E2F1 (aa 1-284) with HA tag

    Depositor

    Kristian Helin

    Article

    Helin et al Genes Dev. 1993 Oct . 7(10):1850-61.

    Insert

    E2F1 1-284 (E2F1 Human)
    

    Expression

    Mammalian

    Available Since

    Jan. 5, 2011

    Availability

    Academic Institutions and Nonprofits only

17. pCS2+MT-hToca-1-MGD

    Plasmid

    #33033

    Depositor

    Marc Kirschner

    Article

    Ho et al Cell. 2004 Jul 23;118(2):203-16.

    Insert

    Toca-1 (FNBP1L Human)
    

    Tags

    6xMyc

    Expression

    Mammalian

    Mutation

    MGD = amino acid substitutions M383I, G384S and D…

    Promoter

    simian CMV IE94

    Available Since

    Nov. 18, 2011

    Availability

    Academic Institutions and Nonprofits only

18. BirA*-HA-NES

    Plasmid

    #214473

    Purpose

    Lentiviral vector for expression in mammalian cells of the proximity ligase BirA(R118G) with a C-terminal HA tag followed by a nuclear exclusion sequence.

    Depositor

    Natalie Ahn

    Article

    Miller et al Mol Biol Cell. 2023 Dec 20:mbcE23060255.

    Insert

    BirA(R118G)
    

    Use

    Lentiviral; Gateway

    Tags

    HA tag and NES (Nuclear Exclusion Sequence)

    Expression

    Mammalian

    Mutation

    R118G

    Promoter

    EF-1a

    Available Since

    March 15, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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19. HS-aPKC-CAAX-GFP

    Plasmid

    #105946

    Purpose

    heat shock inducible transgenesis

    Depositor

    Caren Norden

    Article

    Strzyz et al Dev Cell. 2015 Jan 26;32(2):203-19. doi:

    Insert

    aPKC (Prkcz Rat)
    

    Tags

    CAAX and GFP

    Expression

    Bacterial

    Available Since

    Feb. 6, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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20. pOCC267

    Plasmid

    #118887

    Purpose

    shuttle vector for baculovirus production, using FlashBac bacmid; for recombinant protein with N-terminal HIS6-MBP tag, cleavable with 3C, and N-terminal HALO tag

    Depositor

    Marc Bickle, PEPC MPI CBG

    Article

    Lemaitre et al BMC Biotechnol. 2019 Mar 29;19(1):20. do

    Insert

    NcoI-HIS6-MBP-3C-HALO-NotI-ccdB-AscI-stop-HindIII cassette
    

    Tags

    HIS6-MBP, cleavable with 3C protease; HALO tag

    Expression

    Insect

    Promoter

    polH

    Available Since

    Jan. 2, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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