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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 12,282 results for: LEA

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Showing: 5061 - 5080 of 12282 results

  2. pcDNA3-blueCKAR(T/A)

    Plasmid
    #118473
    Purpose
    Negative-control mutant for blueCKAR biosensor.
    Depositor
    Jin Zhang
    Publication
    Mehta et al Nat Cell Biol. 2018 Sep 24. pii: 10.1038
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    blueCKAR(T/A)
    Tags
    6xHIS, T7 tag (gene 10 leader), and Xpress (TM) t…
    Expression
    Mammalian
    Mutation
    Contains Thr-to-Ala mutation in substrate sequenc…
    Promoter
    CMV
    Available since
    Dec. 6, 2018
    Availability
    Academic Institutions and Nonprofits only
    118473-plasmid-map-sequence-id-230294

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  3. pCI syn iGluf

    Plasmid
    #106121
    Purpose
    Fast indicator for synaptic glutamate imaging (synapsin promoter)
    Depositor
    Katalin Torok
    Publication
    Helassa et al Proc Natl Acad Sci U S A. 2018 May 7. pi
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    iGluSnFR E25D variant
    Tags
    Myc and PDGFR
    Expression
    Mammalian
    Mutation
    Changed Glu 25 to Asp
    Promoter
    synapsin
    Available since
    June 18, 2018
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    1 Article
    106121-plasmid-map-sequence-id-211889

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  5. pcDNA3-FKBP-Venus-173-C

    Plasmid
    #87617
    Purpose
    FKBP-tagged C-terminal Venus (YFP) fragment for BiFC.
    Depositor
    Jin Zhang
    Publication
    Hertel et al Cell Rep. 2016 Jan 12;14(2):390-400. doi
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    FKBP-Venus173-C
    Tags
    6xHis, T7 tag (gene 10 leader), and Xpress (TM) t…
    Expression
    Mammalian
    Promoter
    CMV
    Available since
    May 18, 2018
    Availability
    Academic Institutions and Nonprofits only
    87617-plasmid-map-sequence-id-176212

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  8. pGEM-NLS-BirA-2A-mCherry-SV40pA-FKF

    Plasmid
    #79889
    Purpose
    Donor cassette containing HA-tagged biotin ligase (BirA) with NLS, a Thosea asigna virus peptide (2A), mCherry protein and SV40 polyA tail, followed by FRT site-flanked Kanamycin selection gene
    Depositor
    Tatjana Sauka-Spengler
    Publication
    Trinh et al Cell Rep. 2017 Apr 11;19(2):425-440. doi
    Insert
    HA-tagged NLS-BirA, 2A, mCherry protein and SV40 polyA, followed by FRT site-flanked Kanamycin selection gene
    Expression
    Bacterial
    Available since
    Oct. 11, 2016
    Availability
    Academic Institutions and Nonprofits only
    79889-plasmid-map-sequence-id-150065

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  12. pCDNA6/ERGIC2-WT

    Plasmid
    #51479
    Purpose
    Expresses ERGIC2-WT in mammalian cells
    Depositor
    Simon Kwok
    Publication
    Kwok et al DNA Cell Biol. 2001 Jun;20(6):349-57.
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    ERGIC2-WT (ERGIC2 Human)
    Tags
    none
    Expression
    Mammalian
    Mutation
    none
    Promoter
    CMV
    Available since
    March 6, 2014
    Availability
    Academic Institutions and Nonprofits only

  13. pEMS1385

    Plasmid
    #29184
    Purpose
    Pleiades (Ple) MiniPromoter expression pattern described in Portales-Casamar et al., PNAS, 2010 (PMID: 20807748).
    Depositor
    Elizabeth Simpson
    Publication
    Portales-Casamar et al Proc Natl Acad Sci U S A. 2010 Sep 21. 1
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    Ple186 (S100B Human)
    Use
    Pleiades promoter project [sic, pleaides plieades]
    Tags
    EGFP-NLS
    Available since
    Nov. 15, 2011
    Availability
    Academic Institutions and Nonprofits only
    29184-plasmid-map-sequence-id-13599

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  17. pUC19-U6-BsmBI_epegRNA_entry-mpknot_(LM1140)

    Plasmid
    #228253
    Purpose
    pU6 entry plasmid for cloning prime editor mpknot epegRNAs (requires BsmBI or Esp3I digest and ligation of duplexed oligos)
    Depositor
    Benjamin Kleinstiver
    Publication
    Silverstein et al Nature. 2025 Apr 22. doi: 10.1038/s41586
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    mpknot entry plasmid backbone for epegRNA expression via human U6 promoter
    Expression
    Mammalian
    Mutation
    n/a
    Promoter
    U6
    Available since
    April 23, 2025
    Availability
    Academic Institutions and Nonprofits only
    228253-plasmid-map-sequence-id-451024

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  19. pVSL10

    Plasmid
    #201182
    Purpose
    Bst polymerase large fragment (BF) F710Y/D598A, His6-tagged, HRV 3C-cleavable
    Depositor
    Jack Szostak
    Publication
    Lelyveld et al Science. 2023 Oct 27;382(6669):423-429.
    Insert
    Bst polymerase large fragment (BF) F710Y/D598A
    Tags
    HRV 3C protease cleavage site and His6
    Expression
    Bacterial
    Mutation
    F710Y D598A
    Promoter
    T5-lacO
    Available since
    Sept. 6, 2023
    Availability
    Academic Institutions and Nonprofits only
    201182-plasmid-map-sequence-id-406202

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Showing: 5061 - 5080 of 12282 results