We narrowed to 19,001 results for: Tec

1. pKH-MoHyPer

   Plasmid

   #78267

   Purpose

   Express HyPer-AS sensor that change excitation wavelength with the presence of hydrogen peroxide.

   Depositor

   Nicole Donofrio, Jeffrey Caplan

   Article

   Huang et al Mol Plant Pathol. 2016 Mar 7. doi: 10.11

   Insert

   MoHyper
   

   Use

   Express in magnaporthe

   Expression

   Yeast

   Promoter

   Rp27

   Available Since

   Aug. 9, 2016

   Availability

   Academic Institutions and Nonprofits only

2. fADL-1e-flag-linker-p3

   Plasmid

   #139441

   Purpose

   For cloning and subsequent expression of peptide fragments fused with pIII protein as a part of fd filamentous bacteriophage.

   Depositor

   Alexander Gabibov

   Article

   Ishina et al Int J Mol Sci. 2020 Aug 29;21(17). pii:

   Insert

   linker with pelB-leader, cloning site, flag-tag and p3
   

   Expression

   Bacterial

   Available Since

   Aug. 31, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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3. MSP2266

   Plasmid

   #70705

   Purpose

   Bacterial expression plasmid for SaCas9 & sgRNA targeted to site 2: T7-humanSaCas9-NLS-3xFLAG-T7-Sa-sgRNA(84) #2

   Depositor

   Keith Joung

   Article

   Kleinstiver et al Nat Biotechnol. 2015 Nov 2. doi: 10.1038

   Inserts

   mammalian codon-optimized Staphylococcus aureus Cas9
   SaCas9 sgRNA (+84)
   

   Use

   CRISPR

   Tags

   NLS-3xFLAG

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   Dec. 4, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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4. pDisplay FLIPE-10u

   Plasmid

   #13546

   Depositor

   Wolf Frommer

   Article

   Okumoto et al Proc Natl Acad Sci U S A. 2005 Jun 14. 1

   Insert

   FLIP-E A207R
   

   Tags

   ECFP and Venus

   Expression

   Mammalian

   Mutation

   A207R in binding protein

   Available Since

   Dec. 15, 2006

   Availability

   Academic Institutions and Nonprofits only

   

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5. pAM/CBA-eGFP-miRaSyn(mouse)x3-WPRE-bGHpA

   Plasmid

   #194248

   Purpose

   Expresses 3 miRNAs targeting mouse alpha-Synuclein

   Depositor

   Michael J Fox Foundation MJFF

   Insert

   miRaSynuclein(mouse) (Snca Mouse)
   

   Use

   AAV and RNAi

   Promoter

   CAG

   Available Since

   Jan. 24, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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6. pCellFree_G03 PDGFRB

   Plasmid

   #67130

   Purpose

   Standard for cell-free expression benchmarking.

   Depositor

   Kirill Alexandrov

   Article

   Gagoski et al Biotechnol Bioeng. 2015 Aug 24. doi: 10.

   Insert

   PDGFRB (PDGFRB Human)
   

   Use

   Cell-free expression

   Tags

   EGFP and His

   Promoter

   T7

   Available Since

   Nov. 6, 2015

   Availability

   Academic Institutions and Nonprofits only

7. pCDNA3.1(+)-HaloTag N58-(GGS)X6-hH2A

   Plasmid

   #171785

   Purpose

   Expression of HaloTag N58-(GGS)X6-hH2A in mammalian cells

   Depositor

   Yujie Sun

   Article

   Shao et al Commun Biol. 2021 Mar 19;4(1):378. doi:

   Insert

   HaloTag N58-(GGS)X6-hH2A
   

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   July 26, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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8. Dppa1-T2A-H2B-Halo donor

   Plasmid

   #113118

   Purpose

   Donor plasmid for knock-in T2A-H2B-Halo to the c-terminal of mouse Dppa1 coding sequence

   Depositor

   Janet Rossant

   Article

   Gu et al Nat Biotechnol. 2018 Jun 11. pii: nbt.41

   Insert

   Dppa1 donor region including homology arms and T2A-H2B-Halo
   

   Use

   Synthetic Biology

   Available Since

   Aug. 6, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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9. pTH-PanCen TALE-mVenusN

   Plasmid

   #191395

   Purpose

   TALE fused to N-terminal part of split mVenus

   Depositor

   Albert Jeltsch

   Article

   Lungu et al Nat Commun. 2017 Sep 21;8(1):649. doi: 1

   Insert

   N-terminal part of split mVenus
   

   Use

   TALEN

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   May 31, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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10. p416GAL1-Zipp.-L-IFP-F[1] (Yeast)

    Plasmid

    #52895

    Purpose

    Plasmid+insert encoding GCN4-leucine zipper fused to IFP-F[1] by the linker (GGGGS) X 2. Zipper-L-IFP-F[1] (XbaI/XhoI). Zipper is (XbaI /ClaI). Plasmid confers ampicillin resistance to DH5α.

    Depositor

    Stephen Michnick

    Article

    Tchekanda et al Nat Methods. 2014 Jun;11(6):641-4. doi:

    Insert

    Zipp.-L-IFP-F[1]
    

    Tags

    L-IFP-F[1]

    Expression

    Yeast

    Promoter

    GAL1

    Available Since

    July 22, 2014

    Availability

    Academic Institutions and Nonprofits only

11. TAL2236

    Plasmid

    #36687

    Depositor

    Keith Joung

    Article

    Reyon et al Nat Biotechnol. 2012 Apr 8. doi: 10.1038

    Insert

    CHD4-TALEN_Left (Chd4 Human)
    

    Use

    Mammalian, t7 for in vitro mrna synthesis

    Tags

    3X Flag and WT FOKI

    Promoter

    CMV

    Available Since

    June 26, 2012

    Availability

    Academic Institutions and Nonprofits only

12. pVH232-Tier1-PmPGK1-mRuby2

    Plasmid

    #169523

    Purpose

    Tier-1 vector encoding PmPGK1-driven mRuby2 expression (PmPGK1-mRuby2-pA).

    Depositor

    Martin Fussenegger

    Article

    Haellman et al Metab Eng. 2021 Jul;66:41-50. doi: 10.10

    Insert

    PPGK-driven monomeric red fluorescent protein mRuby2
    

    Expression

    Mammalian

    Promoter

    PPGK

    Available Since

    June 16, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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13. fADL-1e-HA-linker-p3

    Plasmid

    #139440

    Purpose

    For cloning and subsequent expression of peptide fragments fused with pIII protein as a part of fd filamentous bacteriophage

    Depositor

    Alexander Gabibov

    Article

    Ishina et al Int J Mol Sci. 2020 Aug 29;21(17). pii:

    Insert

    linker with pelB-leader, cloning site, HA-tag and p3
    

    Expression

    Bacterial

    Available Since

    Aug. 31, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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14. pCfB6682

    Plasmid

    #106152

    Purpose

    EasyCloneYALI system-based yeast integrative vector for markerfree integration via CRISPR/Cas9 to be used in combination with pCfB6627, integration into Yarrowia lipolytica chromosomal location IntC_2, USER site for cloning, amp resistance

    Depositor

    Irina Borodina

    Article

    Holkenbrink et al Biotechnol J. 2018 Jan 29. doi: 10.1002/

    Type

    Empty backbone

    Expression

    Yeast

    Available Since

    May 11, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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15. pColE1_sgRNA_M7NS_AmpR

    Plasmid

    #119157

    Purpose

    Encodes sgRNA targeting position 6 in pColE1_70a_deGFP_KanR with seven mismatches in the non-seed region

    Depositor

    Vincent Noireaux

    Article

    Collias et al Synth Biol (Oxf). 2019 Jan 21;4(1):ysz00

    Insert

    sgRNA targeting position 6 in pColE1_70a_deGFP_KanR, with seven mismatches in the non-seed region
    

    Use

    Crispr

    Promoter

    J23119

    Available Since

    Nov. 28, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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16. pColE1_sgRNA_M2S_AmpR

    Plasmid

    #119154

    Purpose

    Encodes sgRNA targeting position 6 in pColE1_70a_deGFP_KanR with two mismatches in the seed region

    Depositor

    Vincent Noireaux

    Article

    Collias et al Synth Biol (Oxf). 2019 Jan 21;4(1):ysz00

    Insert

    sgRNA targeting position 6 in pColE1_70a_deGFP_KanR, with two mismatches in the seed region
    

    Use

    Crispr

    Promoter

    J23119

    Available Since

    Nov. 28, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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17. pSR26-2

    Plasmid

    #104335

    Purpose

    Expresses ompR from a TetR reuglated promoter under inducible ATC control.

    Depositor

    Jeffrey Tabor

    Article

    Landry et al Nat Commun. 2018 Apr 12;9(1):1433. doi:

    Insert

    OmpR (ompR Escherichia coli str. K-12 substr. MG1655)
    

    Expression

    Bacterial

    Promoter

    PLTetO1

    Available Since

    April 17, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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18. EK0500 SFFV-mCherry-SGc(RPLP1.s1)cSG-EGFP-(3xMS2) (FLP-IN)

    Plasmid

    #191184

    Purpose

    Expression of RPLP1 sensor in mammalian cells

    Depositor

    Xiaojing Gao

    Article

    Kaseniit et al Nat Biotechnol. 2022 Oct 5. pii: 10.1038

    Insert

    mCherry:RPLP1.s1:EGFP-3xMS2
    

    Expression

    Mammalian

    Mutation

    WT

    Promoter

    SFFV

    Available Since

    Oct. 26, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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19. EK0261 CMV-TO-mCherry-SG(s70587)SG-fs(EGFP) (FLP-IN)

    Plasmid

    #191175

    Purpose

    Mammalian expression of EK0209 with frameshift mutation (EGFP is frameshifted).

    Depositor

    Xiaojing Gao

    Article

    Kaseniit et al Nat Biotechnol. 2022 Oct 5. pii: 10.1038

    Insert

    CMV-TO:mCherry:s70587:EGFP (frameshifted)
    

    Expression

    Mammalian

    Mutation

    EGFP is frameshifted

    Promoter

    CMV-TO

    Available Since

    Oct. 26, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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20. LLP752_L2pV1_AND_B3RT_FRT_Rluc_2IBF_lacZ

    Plasmid

    #192396

    Purpose

    To act as a 2-input state of the AND gate.

    Depositor

    Ryan Lister

    Article

    Lloyd et al Nat Biotechnol. 2022 Jul 4. pii: 10.1038

    Insert

    Act2::FRT-OCS-FRT::B3RT-OCS-B3RT::Rluc
    

    Use

    Synthetic Biology

    Tags

    PEST

    Expression

    Plant

    Available Since

    Nov. 29, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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