We narrowed to 19,027 results for: Tec;

1. pcDNA3.1 FLIPE-10u

   Plasmid

   #13542

   Depositor

   Wolf Frommer

   Article

   Okumoto et al Proc Natl Acad Sci U S A. 2005 Jun 14. 1

   Insert

   FLIP-E A207R
   

   Tags

   ECFP and Venus

   Expression

   Mammalian

   Mutation

   A207R in binding protein

   Available Since

   Dec. 15, 2006

   Availability

   Academic Institutions and Nonprofits only

   

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2. pDisplay FLIPE-1m

   Plasmid

   #13548

   Depositor

   Wolf Frommer

   Article

   Okumoto et al Proc Natl Acad Sci U S A. 2005 Jun 14. 1

   Insert

   FLIP-E A207W
   

   Tags

   ECFP and Venus

   Expression

   Mammalian

   Mutation

   A207W in binding protein

   Available Since

   Dec. 15, 2006

   Availability

   Academic Institutions and Nonprofits only

   

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3. pCfB6684

   Plasmid

   #106154

   Purpose

   EasyCloneYALI system-based yeast integrative vector for markerfree integration via CRISPR/Cas9 to be used in combination with pCfB6631, integration into Yarrowia lipolytica chromosomal location IntD_1, USER site for cloning, amp resistance

   Depositor

   Irina Borodina

   Article

   Holkenbrink et al Biotechnol J. 2018 Jan 29. doi: 10.1002/

   Type

   Empty backbone

   Expression

   Yeast

   Available Since

   May 11, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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4. pCfB6631

   Plasmid

   #106161

   Purpose

   EasyCloneYALI system-based yeast gRNA expression vector carrying a nourseothricin-resistance marker, helps to integrate vector pCfB6684 into Yarrowia lipolytica chromosomal location IntD_1, amp resistance

   Depositor

   Irina Borodina

   Article

   Holkenbrink et al Biotechnol J. 2018 Jan 29. doi: 10.1002/

   Insert

   unknown
   

   Expression

   Yeast

   Available Since

   May 11, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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5. pKH-MoHyPer

   Plasmid

   #78267

   Purpose

   Express HyPer-AS sensor that change excitation wavelength with the presence of hydrogen peroxide.

   Depositor

   Jeffrey Caplan, Nicole Donofrio

   Article

   Huang et al Mol Plant Pathol. 2016 Mar 7. doi: 10.11

   Insert

   MoHyper
   

   Use

   Express in magnaporthe

   Expression

   Yeast

   Promoter

   Rp27

   Available Since

   Aug. 9, 2016

   Availability

   Academic Institutions and Nonprofits only

6. MSP2266

   Plasmid

   #70705

   Purpose

   Bacterial expression plasmid for SaCas9 & sgRNA targeted to site 2: T7-humanSaCas9-NLS-3xFLAG-T7-Sa-sgRNA(84) #2

   Depositor

   Keith Joung

   Article

   Kleinstiver et al Nat Biotechnol. 2015 Nov 2. doi: 10.1038

   Inserts

   mammalian codon-optimized Staphylococcus aureus Cas9
   SaCas9 sgRNA (+84)
   

   Use

   CRISPR

   Tags

   NLS-3xFLAG

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   Dec. 4, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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7. fADL-1e-flag-linker-p3

   Plasmid

   #139441

   Purpose

   For cloning and subsequent expression of peptide fragments fused with pIII protein as a part of fd filamentous bacteriophage.

   Depositor

   Alexander Gabibov

   Article

   Ishina et al Int J Mol Sci. 2020 Aug 29;21(17). pii:

   Insert

   linker with pelB-leader, cloning site, flag-tag and p3
   

   Expression

   Bacterial

   Available Since

   Aug. 31, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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8. pDisplay FLIPE-10u

   Plasmid

   #13546

   Depositor

   Wolf Frommer

   Article

   Okumoto et al Proc Natl Acad Sci U S A. 2005 Jun 14. 1

   Insert

   FLIP-E A207R
   

   Tags

   ECFP and Venus

   Expression

   Mammalian

   Mutation

   A207R in binding protein

   Available Since

   Dec. 15, 2006

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pAM/CBA-eGFP-miRaSyn(mouse)x3-WPRE-bGHpA

   Plasmid

   #194248

   Purpose

   Expresses 3 miRNAs targeting mouse alpha-Synuclein

   Depositor

   Michael J Fox Foundation MJFF

   Insert

   miRaSynuclein(mouse) (Snca Mouse)
   

   Use

   AAV and RNAi

   Promoter

   CAG

   Available Since

   Jan. 24, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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10. pCellFree_G03 PDGFRB

    Plasmid

    #67130

    Purpose

    Standard for cell-free expression benchmarking.

    Depositor

    Kirill Alexandrov

    Article

    Gagoski et al Biotechnol Bioeng. 2015 Aug 24. doi: 10.

    Insert

    PDGFRB (PDGFRB Human)
    

    Use

    Cell-free expression

    Tags

    EGFP and His

    Promoter

    T7

    Available Since

    Nov. 6, 2015

    Availability

    Academic Institutions and Nonprofits only

11. pCDNA3.1(+)-HaloTag N58-(GGS)X6-hH2A

    Plasmid

    #171785

    Purpose

    Expression of HaloTag N58-(GGS)X6-hH2A in mammalian cells

    Depositor

    Yujie Sun

    Article

    Shao et al Commun Biol. 2021 Mar 19;4(1):378. doi:

    Insert

    HaloTag N58-(GGS)X6-hH2A
    

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    July 26, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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12. Dppa1-T2A-H2B-Halo donor

    Plasmid

    #113118

    Purpose

    Donor plasmid for knock-in T2A-H2B-Halo to the c-terminal of mouse Dppa1 coding sequence

    Depositor

    Janet Rossant

    Article

    Gu et al Nat Biotechnol. 2018 Jun 11. pii: nbt.41

    Insert

    Dppa1 donor region including homology arms and T2A-H2B-Halo
    

    Use

    Synthetic Biology

    Available Since

    Aug. 6, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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13. pTH-PanCen TALE-mVenusN

    Plasmid

    #191395

    Purpose

    TALE fused to N-terminal part of split mVenus

    Depositor

    Albert Jeltsch

    Article

    Lungu et al Nat Commun. 2017 Sep 21;8(1):649. doi: 1

    Insert

    N-terminal part of split mVenus
    

    Use

    TALEN

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    May 31, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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14. p416GAL1-Zipp.-L-IFP-F[1] (Yeast)

    Plasmid

    #52895

    Purpose

    Plasmid+insert encoding GCN4-leucine zipper fused to IFP-F[1] by the linker (GGGGS) X 2. Zipper-L-IFP-F[1] (XbaI/XhoI). Zipper is (XbaI /ClaI). Plasmid confers ampicillin resistance to DH5α.

    Depositor

    Stephen Michnick

    Article

    Tchekanda et al Nat Methods. 2014 Jun;11(6):641-4. doi:

    Insert

    Zipp.-L-IFP-F[1]
    

    Tags

    L-IFP-F[1]

    Expression

    Yeast

    Promoter

    GAL1

    Available Since

    July 22, 2014

    Availability

    Academic Institutions and Nonprofits only

15. TAL2236

    Plasmid

    #36687

    Depositor

    Keith Joung

    Article

    Reyon et al Nat Biotechnol. 2012 Apr 8. doi: 10.1038

    Insert

    CHD4-TALEN_Left (Chd4 Human)
    

    Use

    Mammalian, t7 for in vitro mrna synthesis

    Tags

    3X Flag and WT FOKI

    Promoter

    CMV

    Available Since

    June 26, 2012

    Availability

    Academic Institutions and Nonprofits only

16. pVH232-Tier1-PmPGK1-mRuby2

    Plasmid

    #169523

    Purpose

    Tier-1 vector encoding PmPGK1-driven mRuby2 expression (PmPGK1-mRuby2-pA).

    Depositor

    Martin Fussenegger

    Article

    Haellman et al Metab Eng. 2021 Jul;66:41-50. doi: 10.10

    Insert

    PPGK-driven monomeric red fluorescent protein mRuby2
    

    Expression

    Mammalian

    Promoter

    PPGK

    Available Since

    June 16, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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17. pCfB6682

    Plasmid

    #106152

    Purpose

    EasyCloneYALI system-based yeast integrative vector for markerfree integration via CRISPR/Cas9 to be used in combination with pCfB6627, integration into Yarrowia lipolytica chromosomal location IntC_2, USER site for cloning, amp resistance

    Depositor

    Irina Borodina

    Article

    Holkenbrink et al Biotechnol J. 2018 Jan 29. doi: 10.1002/

    Type

    Empty backbone

    Expression

    Yeast

    Available Since

    May 11, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. fADL-1e-HA-linker-p3

    Plasmid

    #139440

    Purpose

    For cloning and subsequent expression of peptide fragments fused with pIII protein as a part of fd filamentous bacteriophage

    Depositor

    Alexander Gabibov

    Article

    Ishina et al Int J Mol Sci. 2020 Aug 29;21(17). pii:

    Insert

    linker with pelB-leader, cloning site, HA-tag and p3
    

    Expression

    Bacterial

    Available Since

    Aug. 31, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pColE1_sgRNA_M7NS_AmpR

    Plasmid

    #119157

    Purpose

    Encodes sgRNA targeting position 6 in pColE1_70a_deGFP_KanR with seven mismatches in the non-seed region

    Depositor

    Vincent Noireaux

    Article

    Collias et al Synth Biol (Oxf). 2019 Jan 21;4(1):ysz00

    Insert

    sgRNA targeting position 6 in pColE1_70a_deGFP_KanR, with seven mismatches in the non-seed region
    

    Use

    Crispr

    Promoter

    J23119

    Available Since

    Nov. 28, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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20. pColE1_sgRNA_M2S_AmpR

    Plasmid

    #119154

    Purpose

    Encodes sgRNA targeting position 6 in pColE1_70a_deGFP_KanR with two mismatches in the seed region

    Depositor

    Vincent Noireaux

    Article

    Collias et al Synth Biol (Oxf). 2019 Jan 21;4(1):ysz00

    Insert

    sgRNA targeting position 6 in pColE1_70a_deGFP_KanR, with two mismatches in the seed region
    

    Use

    Crispr

    Promoter

    J23119

    Available Since

    Nov. 28, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge