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LentiV_Blast

Plasmid

#111887

Purpose

Lentiviral expression of cDNA with blasticidin resistance gene

Depositor

Christopher Vakoc

Publication

Tarumoto et al Blood. 2019 Nov 1. pii: 422697. doi: 10.

Type

Empty backbone

Use

Lentiviral

Tags

FLAG

Expression

Mammalian

Promoter

EFS promoter

Available since

June 10, 2019

Availability

Academic Institutions and Nonprofits only

Cited by

5 Articles

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pHR-PGK-scFv GCN4-sfGFP-VP64

Plasmid

#122133

Purpose

Express a scFv which binds to GCN4, sfGFP and VP64 cassette from the PGK promoter

Depositor

Stanley Qi

Publication

Liu et al Cell Stem Cell. 2018 Sep 28. pii: S1934-

Insert

scFv GCN4-sfGFP-VP64

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

PGK

Available since

May 13, 2019

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pCMV 3xFLAG-LC3A WT

Plasmid

#123089

Purpose

Expresses 3xFLAG-LC3A wild-type in mammalian cells.

Depositor

Robin Ketteler

Publication

Agrotis et al Autophagy. 2019 Jan 21. doi: 10.1080/155

Insert

Microtubule-associated proteins 1A/1B light chain 3A (MAP1LC3A Human)

Tags

3xFLAG

Expression

Mammalian

Promoter

CMV

Available since

May 7, 2019

Availability

Academic Institutions and Nonprofits only

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pHR-PGK-PYL1-sfGFP-Coilin

Plasmid

#122177

Purpose

expression of PYL1-SfGFP-Coilin, lentivirus vector

Depositor

Stanley Qi

Publication

Wang et al Cell. 2018 Nov 15;175(5):1405-1417.e14.

Insert

Coilin (COIL Human)

Use

Lentiviral

Tags

PYL1-SFGFP

Expression

Mammalian

Promoter

PGK

Available since

April 24, 2019

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pGTag-eGFP-SV40

Plasmid

#117813

Purpose

Donor for precise CRISPR directed genomic integration using the GeneWeld method

Depositor

Jeffrey Essner

Publication

Wierson et al Elife. 2020 May 15;9. pii: 53968. doi: 1

Insert

eGFP

Use

Synthetic Biology

Available since

March 15, 2019

Availability

Academic Institutions and Nonprofits only

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pHAGE-TGFB1

Plasmid

#116799

Purpose

Lentiviral expression of TGFB1

Depositor

Gordon Mills, Kenneth Scott

Publication

Ng et al Cancer Cell. 2018 Mar 12;33(3):450-462.e

Insert

TGFB1 (TGFB1 Human)

Use

Lentiviral

Mutation

WT

Available since

March 14, 2019

Availability

Academic Institutions and Nonprofits only

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pHAGE-GATA3

Plasmid

#116747

Purpose

Lentiviral expression of GATA3

Depositor

Gordon Mills, Kenneth Scott

Publication

Ng et al Cancer Cell. 2018 Mar 12;33(3):450-462.e

Insert

GATA3 (GATA3 Human)

Use

Lentiviral

Mutation

WT

Available since

March 14, 2019

Availability

Academic Institutions and Nonprofits only

Cited by

3 Articles

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EF1a_GATA6_P2A_Hygro_Barcode

Plasmid

#120445

Purpose

Barcoded lentiviral vector to express GATA6 in mammalian cells under control of EF1a promoter. Barcoded for transcript detection in single cell RNA-seq.

Depositor

Prashant Mali

Publication

Parekh et al Cell Syst. 2018 Nov 12. pii: S2405-4712(

Insert

GATA6 (GATA6 Human)

Use

Lentiviral

Promoter

EF1a

Available since

Feb. 4, 2019

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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pAAV-hsyn-flex-Voltron

Plasmid

#119035

Purpose

Cre-dependant expression of Voltron in neurons

Depositor

Eric Schreiter

Publication

Abdelfattah et al Science. 2019 Aug 1. pii: science.aav641

Insert

Voltron

Use

AAV

Expression

Mammalian

Promoter

hsyn

Available since

Dec. 19, 2018

Availability

Academic Institutions and Nonprofits only

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pET-28b-T7-bLbCas12a-NLS(nucleoplasmin)-6xHis (RTW645)

Plasmid

#114070

Purpose

T7 promoter bacterial expression plasmid for bacterial codon optimized LbCas12a with C-terminal NLS and His-tag

Depositor

Keith Joung, Benjamin Kleinstiver

Publication

Kleinstiver et al Nat Biotechnol. 2019 Feb 11. pii: 10.103

Insert

bacteria codon optimized LbCas12a with C-terminal NLS and His-tag

Tags

NLS(nucleoplasmin) and 6xHis

Expression

Bacterial

Available since

Dec. 11, 2018

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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pLBH559_Tet-HisCas14a1Locus

Plasmid

#112502

Purpose

Expresses 10xHis-Cas14a1 locus (including crRNA and tracrRNA) under control of a tetracycline inducible promoter

Depositor

Jennifer Doudna

Publication

Harrington et al Science. 2018 Oct 18. pii: science.aav42

Insert

Cas14a1

Use

CRISPR

Tags

6xHis-TEV

Expression

Bacterial

Promoter

Tet

Available since

Oct. 26, 2018

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pWPI-E2F1

Plasmid

#114296

Purpose

Constitutive expression of the E2F1 protein

Depositor

Patrick Salmon

Publication

Rady et al Cell Cycle. 2013 Aug 15;12(16):2691-702.

Insert

E2F1 (E2F1 Human)

Use

Lentiviral

Available since

Oct. 10, 2018

Availability

Academic Institutions and Nonprofits only

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pEBTet_NADP-Snifit-cyto

Plasmid

#113917

Purpose

Plasmid encoding the cytosolic localization of the FRET semi-synthetic biosensor measuring the ratio NADPH/NADP+ in mammalian cells

Depositor

Kai Johnsson

Publication

Sallin et al eLife. 2018 May 29;7. pii: 32638. doi: 1

Insert

NADP-Snifit

Expression

Mammalian

Promoter

CMV TetO2

Available since

Sept. 19, 2018

Availability

Academic Institutions and Nonprofits only

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pLenti-BE4GamRA-P2A-Puro

Plasmid

#112673

Purpose

Lentivirus for constitutive expression of BE4GamRA in mammalian cells (codon optimized)

Depositor

Lukas Dow

Publication

Zafra et al Nat Biotechnol. 2018 Jul 3. pii: nbt.419

Insert

BE4GamRA

Use

Lentiviral

Tags

FLAG

Mutation

NLS sequence at the N-terminus and D10A

Promoter

EF1s

Available since

July 30, 2018

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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EasyFusion Halo-mAID

Plasmid

#112852

Purpose

General donor vector with Halo-mAID coding sequence flanking by restriction sites for generating knock-in donor for Auxin inducible degradation of Halo tagged endogenous protein

Depositor

Janet Rossant

Publication

Gu et al Nat Biotechnol. 2018 Jun 11. pii: nbt.41

Insert

Halo-mAID

Use

Synthetic Biology

Promoter

No

Available since

July 26, 2018

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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pAJM.677

Plasmid

#108530

Purpose

AraCAM + AraE + PBAD-YFP Reporter. Ara sensor for use in non-Marionette strain.

Depositor

Christopher Voigt

Publication

Meyer et al Nat Chem Biol. 2018 Nov 26. pii: 10.1038

Insert

AraCAM + AraE + PBAD-YFP

Expression

Bacterial

Mutation

AraC: L133F E165L E169G C280L

Available since

July 17, 2018

Availability

Academic Institutions and Nonprofits only

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pAJM.011

Plasmid

#108529

Purpose

TetR + PTet*-YFP Reporter. aTc sensor for use in non-Marionette strain.

Depositor

Christopher Voigt

Publication

Meyer et al Nat Chem Biol. 2018 Nov 26. pii: 10.1038

Insert

TetR + PTet*-YFP

Expression

Bacterial

Available since

June 29, 2018

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pLS-mP-Luc

Plasmid

#106253

Purpose

Luciferase lentivirus based enhancer assay vector

Depositor

Nadav Ahituv

Insert

Luciferase

Use

Lentiviral and Luciferase

Promoter

Derived from pGL4.23

Available since

May 17, 2018

Availability

Academic Institutions and Nonprofits only

Cited by

7 Articles

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pTol2-UAS-zArchon1-KGC-EGFP-ER2

Plasmid

#108427

Purpose

Codon-optimized Archon1 fluorescent voltage reporter in zebrafish expression plasmid

Depositor

Edward Boyden

Publication

Piatkevich et al Nat Chem Biol. 2018 Feb 26. pii: 10.1038

Insert

zArchon1-KGC-EGFP-ER2

Use

Zebrafish

Promoter

UAS

Available since

May 9, 2018

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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pcDNA4-GFP-Vinculin

Plasmid

#107153

Purpose

mammalian expression

Depositor

Tina Izard

Publication

Chinthalapudi et al J Cell Biol. 2014 Dec 8;207(5):643-56. d

Insert

Vinculin (VCL Human)

Tags

(His)10-GFP

Expression

Mammalian

Promoter

CMV

Available since

May 8, 2018

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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