We narrowed to 10,197 results for: pcDNA 3.1

1. pcDNA3-FluoSTEP-AKAR (C/Y)

   Plasmid

   #181862

   Purpose

   FluoSTEP-AKAR using split CFP and cpVenus(E172) as the FRET donor and acceptor. Must pair with GFP11-tagged POI to reconstitute donor fluorescence.

   Depositor

   Jin Zhang

   Article

   Tenner et al Sci Adv. 2021 May 21;7(21). pii: 7/21/ea

   Insert

   FluoSTEP-AKAR (C-Y)
   

   Tags

   CFP(1-10) and cpVenus(E127)

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   May 26, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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2. pcDNA-Prom1 Del AA 6

   Plasmid

   #184024

   Purpose

   Mammalian Flag tagged expression clone for the mouse Prom1 splicing variant SV8 (GenBank accession BC028286). Deletion of amino acids 689 to 694.

   Depositor

   Peter Stoilov

   Article

   Matalkah et al PLoS One. 2022 Oct 10;17(10):e0274958. d

   Insert

   Prom1 SV8 Del AA 6 (Prom1 Mouse)
   

   Tags

   Flag

   Expression

   Mammalian

   Mutation

   Deletion of amino acids 689 to 694

   Available Since

   May 20, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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3. pcDNA-Ikkb-FLAG(K44A)

   Plasmid

   #23299

   Depositor

   Warner Greene

   Article

   Geleziunas et al Mol Cell Biol. 1998 Sep . 18(9):5157-65.

   Insert

   Ikkbeta (IKBKB Human)
   

   Tags

   Flag

   Expression

   Mammalian

   Mutation

   K44A

   Available Since

   April 16, 2010

   Availability

   Academic Institutions and Nonprofits only

4. pcDNA3-HLA-cMyc-EcopT1R1

   Plasmid

   #113962

   Purpose

   mammalian expression plasmid for c-myc-tagged E. coli codon-optimized human T1R1 with signal peptide of HLA class I histocompatibility antigen A-2 alpha chain

   Depositor

   Erik Procko

   Article

   Park et al J Biol Chem. 2019 Feb 5. pii: RA118.0061

   Insert

   T1R1 (TAS1R1 Human)
   

   Tags

   Signal/leader sequence from HLA class I histocomp…

   Expression

   Mammalian

   Mutation

   truncate N-terminal 24 residues

   Available Since

   Feb. 8, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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5. pcDNA3-FLAG-mTET2 (N1041)

   Plasmid

   #89737

   Purpose

   Expresses the N1041 truncation to mouse TET2 in mammalian cells

   Depositor

   Yue Xiong

   Article

   Nakagawa et al Mol Cell. 2015 Jan 22;57(2):247-60. doi:

   Insert

   TET2 (Tet2 Mouse)
   

   Tags

   Flag

   Expression

   Mammalian

   Mutation

   N-term 1041 truncation of mouse TET2 (contains fi…

   Available Since

   May 3, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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6. pcDNA3-FLAG-mTET2 (N500)

   Plasmid

   #89897

   Purpose

   Expresses mouse TET2 N500 truncation in mammalian cells

   Depositor

   Yue Xiong

   Article

   Nakagawa et al Mol Cell. 2015 Jan 22;57(2):247-60. doi:

   Insert

   TET2 (Tet2 Mouse)
   

   Tags

   Flag

   Expression

   Mammalian

   Mutation

   N-term 500 truncation of mouse TET2 (contains fir…

   Available Since

   May 3, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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7. pcDNA-Cx43-S279/282A

   Plasmid

   #60712

   Purpose

   mammalian expression of rat Cx43 S279/282A mutant

   Depositor

   Parmender Mehta

   Article

   Johnson et al Mol Biol Cell. 2013 Mar;24(6):715-33. do

   Insert

   Cx43 (Gja1 Rat)
   

   Expression

   Mammalian

   Mutation

   S279/282A

   Promoter

   CMV

   Available Since

   July 6, 2015

   Availability

   Academic Institutions and Nonprofits only

8. pcDNA-Cx43-S279/282D

   Plasmid

   #60714

   Purpose

   mammalian expression of rat Cx43 S279/282D mutant

   Depositor

   Parmender Mehta

   Article

   Johnson et al Mol Biol Cell. 2013 Mar;24(6):715-33. do

   Insert

   Cx43 (Gja1 Rat)
   

   Expression

   Mammalian

   Mutation

   S279/282D

   Promoter

   CMV

   Available Since

   July 6, 2015

   Availability

   Academic Institutions and Nonprofits only

9. pcDNA3.2-Cx43-M100L-siResist

   Plasmid

   #49854

   Purpose

   Encodes human connexin 43 with M100 mutated to L and wobble mutations conferring resistance against an siRNA

   Depositor

   Robin Shaw

   Article

   Smyth et al Cell Rep. 2013 Nov 5. pii: S2211-1247(13

   Insert

   Connexin 43 (GJA1 Human)
   

   Expression

   Mammalian

   Mutation

   Methionine100 mutated to Leucine. Contains severa…

   Promoter

   CMV

   Available Since

   Jan. 17, 2014

   Availability

   Academic Institutions and Nonprofits only

10. pcDNA3-CXCL12-sfGFP

    Plasmid

    #98961

    Purpose

    Mammalian expression plasmid for superfolder GFP-fused human chemokine CXCL12

    Depositor

    Erik Procko

    Article

    Heredia et al J Immunol. 2018 Apr 20. pii: jimmunol.18

    Insert

    CXCL12 (CXCL12 Human)
    

    Tags

    Superfolder GFP

    Expression

    Mammalian

    Mutation

    Full-length, wildtype

    Promoter

    CMV

    Available Since

    April 24, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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11. pcDNA-dCas9-VP64

    Plasmid

    #47107

    Purpose

    Expresses inactivated S. pyogenes dCas9 (D10A, H840A) fused to VP64 transactivator domain in mammalian cells

    Depositor

    Charles Gersbach

    Article

    Perez-Pinera et al Nat Methods. 2013 Jul 25. doi: 10.1038/n

    Insert

    dCas9-VP64
    

    Use

    CRISPR

    Tags

    Flag, HA, SV40 NLS, and VP64

    Expression

    Mammalian

    Mutation

    D10A, H840A (catalytically inactive)

    Promoter

    CMV

    Available Since

    Nov. 14, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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12. pcDNA3-FLAG-mTET2

    Plasmid

    #89735

    Purpose

    Expresses wildtype mouse TET2 in mammalian cells

    Depositor

    Yue Xiong

    Article

    Nakagawa et al Mol Cell. 2015 Jan 22;57(2):247-60. doi:

    Insert

    TET2 (Tet2 Mouse)
    

    Tags

    Flag

    Expression

    Mammalian

    Available Since

    May 3, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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13. pCDNA3-DCX-TurboID-V5

    Plasmid

    #190740

    Purpose

    Mammalian expression of DCX fusion to V5-tagged TurboID on microtubules (DCX as the microtubule-targeting tag)

    Depositor

    Jeremy Baskin

    Article

    Cao et al ACS Chem Biol. 2022 Aug 11. doi: 10.1021

    Insert

    doublecortin (DCX Human)
    

    Tags

    TurboID and V5

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Oct. 31, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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14. pcDNA3 proHB-EGF NC

    Plasmid

    #11605

    Depositor

    Rosalyn Adam

    Article

    Kim et al Cancer Res. 2005 Sep 15. 65(18):8242-9.

    Insert

    HB-EGF (HBEGF Human)
    

    Expression

    Mammalian

    Mutation

    Non-cleavable HB-EGF: Pro149 to Thr160 replaced w…

    Available Since

    April 26, 2006

    Availability

    Academic Institutions and Nonprofits only

15. pcDNA -CLCN3-3xFlag

    Plasmid

    #225506

    Purpose

    CLCN3 expression vector

    Depositor

    Wade Harper

    Article

    Gonzalez-Lozano et al Nature. 2025 Jul;643(8070):252-261. doi:

    Insert

    CLCN3 (CLCN3 Human)
    

    Tags

    3xFlag

    Expression

    Mammalian

    Available Since

    May 14, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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16. pcDNA3-HA-DNM2-R465W

    Plasmid

    #155262

    Purpose

    Mammalian expression of rat DNM2 fused to HA

    Depositor

    David Rubinsztein

    Article

    Puri et al Dev Cell. 2020 Apr 20;53(2):154-168.e6.

    Insert

    dynamin 2 (Dnm2 Rat)
    

    Tags

    HA

    Expression

    Mammalian

    Mutation

    Arginine 465 to Tryptophan

    Available Since

    May 14, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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17. pcDNA flag-Msi1

    Plasmid

    #184029

    Purpose

    Msi1 clone with N-terminal flag tag

    Depositor

    Peter Stoilov

    Article

    Matalkah et al Commun Biol. 2022 Sep 24;5(1):1011. doi:

    Insert

    Msi1 (Msi1 Mouse)
    

    Tags

    Flag

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    May 27, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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18. pcDNA3-LYRa11-RBD-8h

    Plasmid

    #161822

    Purpose

    Mammalian expression plasmid for RBD of bat coronavirus isolate LYRa11

    Depositor

    Erik Procko

    Article

    Chan et al Sci Adv. 2021 Feb 17;7(8). pii: 7/8/eabf

    Insert

    Receptor-binding domain (RBD) of spike protein S
    

    Tags

    GSG linker and 8his tag and HA leader sequence

    Expression

    Mammalian

    Mutation

    Human codon optimized RBD (a.a. T324-D522)

    Promoter

    CMV

    Available Since

    Nov. 10, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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19. pcDNA3-RsSHC014-RBD-8h

    Plasmid

    #161826

    Purpose

    Mammalian expression plasmid for RBD of bat coronavirus isolate RsSHC014

    Depositor

    Erik Procko

    Article

    Chan et al Sci Adv. 2021 Feb 17;7(8). pii: 7/8/eabf

    Insert

    Receptor-binding domain (RBD) of spike protein S
    

    Tags

    GSG linker and 8his tag and HA leader sequence

    Expression

    Mammalian

    Mutation

    Human codon optimized RBD (a.a. T321-D519)

    Promoter

    CMV

    Available Since

    Nov. 10, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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20. pcDNA3-SARS-1-RBD-8h

    Plasmid

    #161821

    Purpose

    Mammalian expression plasmid for RBD of SARS-CoV-1 (Urbani strain)

    Depositor

    Erik Procko

    Article

    Chan et al Sci Adv. 2021 Feb 17;7(8). pii: 7/8/eabf

    Insert

    Receptor-binding domain (RBD) of spike protein S
    

    Tags

    GSG linker and 8his tag and HA leader sequence

    Expression

    Mammalian

    Mutation

    Human codon optimized RBD (a.a. T320-D518)

    Promoter

    CMV

    Available Since

    Nov. 10, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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