We narrowed to 25,510 results for: CHI

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pX330a dCas9-KRAB

Plasmid

#92361

Purpose

Modified CAG promoter-containing vector for ubiquitous expression of catalytically inactive Cas9 fused to KRAB repressor domain. For targeted enhancer inactivation in chicken embryos.

Depositor

Tatjana Sauka-Spengler

Article

Williams et al Development. 2018 Feb 23;145(4). pii: de

Insert

dCas9-KRAB

Use

CRISPR

Expression

Mammalian

Promoter

CAG

Available Since

Aug. 24, 2017

Availability

Academic Institutions and Nonprofits only

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pL6puro-JeT-pmRevErbA-Electra2-NLS-Pest

Plasmid

#240111

Purpose

A lentiviral (blue) fluorescent reporter of circadian rhythm, based on the murine Nr1d1-gene (REVERBa protein), expression enhanced by synthtic JeT promoter.

Depositor

Achim Kramer

Article

Gabriel et al bioRxiv 2025.06.30.661926

Insert

murine Nr1d1 promoter+Electra2-NLS-Pest-Reporter (Nr1d1 Mouse)

Use

Lentiviral

Mutation

added JeT Promoter

Available Since

Aug. 4, 2025

Availability

Academic Institutions and Nonprofits only

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pDONR221 CARD8 FIIND S297A

Plasmid

#169966

Purpose

pDONR221 for Gateway shuttling. Contains the autoprocessing-deficient CARD8 FIIND (L162M-P434, no stop codon)

Depositor

Daniel Bachovchin

Article

Sharif et al Immunity. 2021 Jul 13;54(7):1392-1404.e1

Insert

CARD8 (CARD8 Human)

Use

Gateway Cloning

Mutation

S297A

Available Since

May 12, 2021

Availability

Academic Institutions and Nonprofits only

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3xFLAG-dCas9/pMXs-puro

Plasmid

#51240

Purpose

Expresses 3xFLAG-dCas9 in mammalian cells for enChIP analysis to purify specific genomic regions of interest.

Depositor

Hodaka Fujii

Article

Fujita et al PLoS One. 2014 Jul 22;9(7):e103084. doi:

Insert

3xFLAG-dCas9

Use

CRISPR and Retroviral

Tags

3xFLAG tag and NLS (nuclear localization signal)

Expression

Mammalian

Mutation

human codon-optimized, D10A + H840A

Promoter

LTR

Available Since

Aug. 14, 2014

Availability

Academic Institutions and Nonprofits only

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pCMV-dLight1.4

Plasmid

#111057

Purpose

Expresses dLight1.4 in mammalian cells

Depositor

Lin Tian

Article

Patriarchi et al Science. 2018 Jun 29;360(6396). pii: sci

Insert

dLight1.4

Tags

Flag tag

Expression

Mammalian

Available Since

July 20, 2020

Availability

Academic Institutions and Nonprofits only

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pCMV-dLight1.5

Plasmid

#111058

Purpose

Expresses dLight1.5 in mammalian cells

Depositor

Lin Tian

Article

Patriarchi et al Science. 2018 Jun 29;360(6396). pii: sci

Insert

dLight1.5

Tags

Flag tag

Expression

Mammalian

Available Since

July 23, 2019

Availability

Academic Institutions and Nonprofits only

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dTAG NLRP1

Plasmid

#166825

Purpose

dTAG (degron tag) NLRP1 for ligand-controlled N-terminal degradation

Depositor

Daniel Bachovchin

Article

Hollingsworth et al Nature. 2021 Mar 17. pii: 10.1038/s41586

Insert

NLRP1 (NLRP1 Human)

Expression

Mammalian

Promoter

hPGK

Available Since

March 23, 2021

Availability

Academic Institutions and Nonprofits only

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pcDNA3.2/V5 mmu-mir-375

Plasmid

#26336

Depositor

David Bartel

Article

Chiang et al Genes Dev. 2010 May 15. 24(10):992-1009.

Insert

mmu-mir-375 (Mir375 Mouse)

Expression

Mammalian

Available Since

Sept. 29, 2010

Availability

Academic Institutions and Nonprofits only
pGEX-4T1_ATG4B_BamHI_NotI

Plasmid

#190862

Purpose

Plasmid for the expression and purification of of ATG4B. Internal reference: SMC1392

Depositor

Sascha Martens

Article

Fracchiolla et al J Cell Biol. 2020 Jul 6;219(7). pii: 151

Insert

ATG4B (ATG4B Human)

Tags

GST, Thrombin Cleavage site

Expression

Bacterial

Available Since

Sept. 29, 2022

Availability

Academic Institutions and Nonprofits only

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pBAD_sfGFP3TCG4TAG

Plasmid

#174518

Purpose

Recoded sfGFP reporter with the codons TCG and TAG inserted at positions 3 and 4, respectively.

Depositor

Jason W Chin

Article

Robertson et al Science. 2021 Jun 4;372(6546):1057-1062.

Insert

sfGFP

Tags

6 x His

Expression

Bacterial

Mutation

Recoded. TCG and TAG codons were introduced in po…

Promoter

araBAD

Available Since

Oct. 18, 2021

Availability

Academic Institutions and Nonprofits only

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pAAV-hSynapsin1-RdLight1

Plasmid

#125708

Purpose

Expresses the red genetically encoded dopamine sensor RdLight1 in mammalian neurons.

Depositor

Lin Tian

Article

Patriarchi et al Nat Methods. 2020 Nov;17(11):1147-1155.

Has Service

AAV9

Insert

RdLight1

Use

AAV

Tags

Flag tag

Promoter

hSynapsin1

Available Since

Aug. 5, 2021

Availability

Academic Institutions and Nonprofits only

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pMRX-INU-FLAG-RTN3L

Plasmid

#128264

Purpose

Expresses RTN3L in mammalian cells

Depositor

Noboru Mizushima

Article

Chino et al Mol Cell. 2019 Apr 11. pii: S1097-2765(1

Insert

RTN3 (RTN3 Human)

Use

Retroviral

Tags

FLAG

Expression

Mammalian

Promoter

CMV

Available Since

July 26, 2019

Availability

Academic Institutions and Nonprofits only

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pDONR P2R-P3-SV40 polyA signal

Plasmid

#48627

Purpose

Contributes a cassette containing an SV40 polyA signal as the 3’-module during MultiSite Gateway cloning of chimeric cDNAs, when a peptide module at the C-terminal end is not required.

Depositor

Anna Planas, Tomas Santalucia

Article

Buj et al BMC Mol Biol. 2013 Aug 20;14(1):18. doi:

Insert

SV40 early polyadenylation signal cassette

Use

Gateway Cloning

Promoter

none

Available Since

April 9, 2014

Availability

Academic Institutions and Nonprofits only

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pDOXOFF (STOP)99xGly

Plasmid

#211349

Purpose

DOX-regulated lentiviral expression of 99xGly-EGFP-3xHA lacking initiation codon

Depositor

Raghu Chivukula

Article

Yang et al Science. 2025 Jul 17;389(6757):eado2403.

Insert

(STOP)99xGly-EGFP-3xHA

Use

Lentiviral

Tags

EGFP-3xHA

Mutation

Start (ATG) codon replaced with stop (TAG) codon

Available Since

Oct. 28, 2025

Availability

Academic Institutions and Nonprofits only

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pRS0035

Plasmid

#131125

Purpose

Mammalian Target-AID expression vector

Depositor

Nozomu Yachie

Article

Sakata et al Nat Biotechnol. 2020 Jun 1. pii: 10.1038

Insert

nCas9-PmCDA1-UGI

Use

CRISPR

Tags

PmCDA1 and UGI

Expression

Mammalian

Mutation

D10A in SpCas9

Promoter

CMV promoter

Available Since

Sept. 23, 2019

Availability

Academic Institutions and Nonprofits only

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CAG-Flex-TC66T

Plasmid

#48331

Purpose

Cre-dependant TVA receptor-mCherry fusion with a mutation (66T) in TVA

Depositor

Liqun Luo

Article

Miyamichi et al Neuron. 2013 Dec 4;80(5):1232-45. doi: 1

Insert

TVA receptor mCherry fusion with the 66T mutation

Use

AAV and Cre/Lox

Tags

mCherry fusion to TVA receptor

Expression

Mammalian

Mutation

66T

Promoter

CAG

Available Since

Dec. 6, 2013

Availability

Academic Institutions and Nonprofits only

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pCLX-G4E1b-R1DESTR2-MIFE-PGK-mCherry

Plasmid

#114322

Purpose

Mifepristone-inducible lentiviral backbone for Gateway cloning of Gene insert together with constitutive mCherry expression

Depositor

Patrick Salmon

Article

Bocchi et al Nat Commun. 2017 Oct 27;8(1):1158. doi:

Type

Empty backbone

Use

Lentiviral

Available Since

Sept. 11, 2018

Availability

Academic Institutions and Nonprofits only

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pEGFP-N1-Cdc42BPB

Plasmid

#50759

Purpose

expression of EGFP tagged Cdc42BPB

Depositor

Naoki Mochizuki

Article

Ando et al J Cell Biol. 2013 Sep 16;202(6):901-16.

Insert

Cdc42BPB (CDC42BPB Human)

Tags

EGFP

Expression

Mammalian

Promoter

CMV

Available Since

Jan. 14, 2014

Availability

Academic Institutions and Nonprofits only

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pAAV-CAG-RdLight1

Plasmid

#125707

Purpose

Expresses the red genetically encoded dopamine sensor RdLight1 in mammalian cells.

Depositor

Lin Tian

Article

Patriarchi et al Nat Methods. 2020 Nov;17(11):1147-1155.

Has Service

AAV9

Insert

dLight1.3b

Use

AAV

Tags

Flag tag

Expression

Mammalian

Promoter

CAG

Available Since

Aug. 12, 2021

Availability

Academic Institutions and Nonprofits only

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pLenti6/V5-DEST-MTHFD2

Plasmid

#31213

Depositor

Lynda Chin

Article

Scott et al Cancer Cell. 2011 Jul 12;20(1):92-103.

Insert

MTHFD2 (MTHFD2 Human)

Use

Lentiviral

Tags

V5

Expression

Mammalian

Available Since

July 29, 2011

Availability

Academic Institutions and Nonprofits only

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