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We narrowed to 24,030 results for: CHI

Showing: 5101 - 5120 of 24030 results

  1. pcDNA C12 ecDHFR DD YFP HA

    Plasmid
    #192815
    Purpose
    Expresses an N-terminal enhanced destabilizing domain (DD) version of E. coli DHFR with higher basal turnover in mammalian systems. Contains missense mutations W74R/T113S/E120D/Q146L.
    Depositor
    John Hulleman
    Article
    Nakahara et al ACS Chem Biol. 2022 Sep 19. doi: 10.1021
    Insert
    E. coli dihydrofolate reductase
    Use
    Tags
    enhanced yellow fluorescent protein and hemagglut…
    Expression
    Mammalian
    Mutation
    W74R/T113S/E120D/Q146L
    Promoter
    CMV
    Available since
    Nov. 14, 2022
    Availability
    Academic Institutions and Nonprofits only
    192815-plasmid-map-sequence-id-377240

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  3. pAGT8163 (AATG_ttLbCas12a-i_TTCG)

    Plasmid
    #192453
    Purpose
    intronized ttLbCas12a for in planta expression
    Depositor
    Tom Schreiber
    Article
    Schindele et al Plant Biotechnol J. 2023 Mar;21(3):457-4
    Insert
    temperature-tolerant LbCas12a with introns (BsaI-flanked Golden Gate module, MoClo L0)
    Use
    CRISPR and Synthetic Biology; Moclo compatible le…
    Tags
    Expression
    Mutation
    temperature-tolerant mutation (D156R)
    Promoter
    Available since
    Dec. 6, 2022
    Availability
    Academic Institutions and Nonprofits only
    192453-plasmid-map-sequence-id-379029

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  4. pCW57-CMV-MAAP2

    Plasmid
    #218244
    Purpose
    1) Ectopic expression of MAAP2 protein in mammalian cells. 2) Packaging MAAP2 sequence into lentivirus for stable expression in mammalian cells.
    Depositor
    David Schaffer
    Article
    Schieferecke et al Mol Ther. 2024 Feb 7;32(2):340-351. doi:
    Insert
    Membrane-Associated Accessory Protein from Adeno-Associated Virus 2 driven by CMV promoter
    Use
    Lentiviral
    Tags
    Expression
    Mutation
    Promoter
    CMV
    Available since
    Oct. 18, 2024
    Availability
    Academic Institutions and Nonprofits only
    218244-plasmid-map-sequence-id-449684

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  9. pBpaRS3tRNA

    Plasmid
    #197102
    Purpose
    To express the p-benzoylphenylalanine-specific variant of Methanocaldococcus jannaschii TyrRS and its cognate amber suppressor tRNA and allow UAG codon to be translated into p-benzoylphenylalanine.
    Depositor
    Kensaku Sakamoto
    Article
    Sato et al Biochemistry. 2011 Jan 18;50(2):250-7. d
    Insert
    TyrRS variant, amber suppressor tRNA
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Gly32, Ser107, Thr158, Ser159, Arg286 (TyrRS)
    Promoter
    Available since
    June 15, 2023
    Availability
    Academic Institutions and Nonprofits only
    197102-plasmid-map-sequence-id-388022

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  14. pDule-3-nitroTyrosine (5B)

    Plasmid
    #85498
    Purpose
    Plasmid for incorporating the non-canonical amino acid 3-nitroTyrosine with the Mj 3NY (5B) synthetase and cognate amber suppressing tRNA in Ecoli.
    Depositor
    Ryan Mehl
    Article
    Cooley et al Biochemistry. 2014 Apr 1;53(12):1916-24.
    Insert
    3-nitroTyrosine tRNA synthetase and cognate amber suppressing tRNA derived from M. jannaschii Tyrosine synthetase/tRNA system
    Use
    Tags
    None
    Expression
    Bacterial
    Mutation
    Y32H H70C D158S I159A L162R
    Promoter
    lpp (constitutive)
    Available since
    Jan. 10, 2017
    Availability
    Academic Institutions and Nonprofits only
    85498-plasmid-map-sequence-id-169862

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  16. 3xFLAG-dCas9/pCMV-7.1

    Plasmid
    #47948
    Purpose
    Expresses 3xFLAG-dCas9 in mammalian cells for enChIP analysis to purify specific genomic regions of interest.
    Depositor
    Hodaka Fujii
    Article
    Fujita et al Biochem Biophys Res Commun. 2013 Sep 13;
    Insert
    3xFLAG-dCas9
    Use
    CRISPR
    Tags
    3xFLAG and NLS
    Expression
    Mammalian
    Mutation
    human codon-optimized, D10A + H840A (catalyticall…
    Promoter
    CMV
    Available since
    Sept. 13, 2013
    Availability
    Academic Institutions and Nonprofits only
    47948-plasmid-map-sequence-id-68812

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  18. pLenti-BLRR

    Plasmid
    #158958
    Purpose
    Bioluminescent repair reporter (BLRR) for DNA double strand break (DSB) repairs: homology-directed repair (HDR) and non-homologous end joining (NHEJ).
    Depositor
    Charles P. Lai
    Article
    Chien et al Nucleic Acids Res. 2020 Aug 14. pii: 589
    Insert
    BLRR
    Use
    Lentiviral
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    Nov. 10, 2020
    Availability
    Academic Institutions and Nonprofits only
    158958-plasmid-map-sequence-id-316701

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Showing: 5101 - 5120 of 24030 results