We narrowed to 13,833 results for: Cas9

1. pMLS234

   Plasmid

   #73682

   Purpose

   SapTrap 3-site Destination vector for N-terminal GFP tagging with embedded Cbr-unc-119 selectable marker

   Depositor

   Erik Jorgensen

   Article

   Schwartz et al Genetics. 2016 Feb 2. pii: genetics.115.

   Insert

   GFP + Cbr-unc-119
   

   Use

   CRISPR and Cre/Lox

   Tags

   GFP

   Expression

   Worm

   Mutation

   Promoter

   Available since

   April 20, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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2. PM-TD!TB

   Plasmid

   #48656

   Purpose

   Bacterial TD crRNA expression: targets TD to protospacer B (ACTTTAAAAGTATTCGCCAT), p15A/chloramphenicol

   Depositor

   George Church

   Article

   Esvelt et al Nat Methods. 2013 Sep 29. doi: 10.1038/n

   Insert

   Bacterial TD crRNA to prototspacer B
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   Promoter

   J23100 promoter

   Available since

   Oct. 17, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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3. pmCherry-U6-CACNG3

   Plasmid

   #140590

   Purpose

   Expresses a gRNA and mCherry in mammalian cells

   Depositor

   Feng Zhang

   Article

   Schmid-Burgk et al Mol Cell. 2020 Mar 17. pii: S1097-2765(2

   Insert

   gRNA CACNG3
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   Promoter

   Available since

   May 21, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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4. pmCherry-U6-ALDH1A3

   Plasmid

   #140589

   Purpose

   Expresses a gRNA and mCherry in mammalian cells

   Depositor

   Feng Zhang

   Article

   Schmid-Burgk et al Mol Cell. 2020 Mar 17. pii: S1097-2765(2

   Insert

   gRNA ALDH1A3
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   Promoter

   Available since

   May 21, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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5. pGEM-gate

   Plasmid

   #113758

   Purpose

   Gateway destination vector only containing the Gateway cassette and pGEM-T Easy backbone. This can be used with Gateway-compatible Homology-Directed Repair.

   Depositor

   Magdalena Bezanilla

   Article

   Mallett et al Plant Direct. 2019 Sep 12;3(9):e00168. d

   Insert

   gateway cassette
   

   Use

   Gateway destination vector

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Sept. 24, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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6. pHu6-gRNA-NT1

   Plasmid

   #81203

   Purpose

   hU6 expression of gRNA NT1

   Depositor

   David Liu

   Article

   Chaikind et al Nucleic Acids Res. 2016 Aug 11. pii: gkw

   Insert

   hU6 expression of gRNA NT1
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6

   Available since

   Nov. 4, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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7. pX330_HR_Prnp_3

   Plasmid

   #78621

   Purpose

   pX330 vector encoding SpCas9 and a chimeric guide RNA targeting Prnp coding sequence

   Depositor

   Walker Jackson

   Article

   Kaczmarczyk et al PLoS One. 2016 Apr 29;11(4):e0154604. do

   Insert

   pX330_HR_Prnp_3
   

   Use

   CRISPR and Mouse Targeting

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6

   Available since

   July 19, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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8. MYC sgRNA5

   Plasmid

   #100557

   Purpose

   Expresses MYC sgRNA5. Target sequence GAGAGGCAGAGGGAGCGAGC

   Depositor

   Dustin Maly

   Article

   Rose et al Nat Methods. 2017 Jul 24. doi: 10.1038/n

   Insert

   MYC sgRNA5
   

   Use

   Tags

   Expression

   Mutation

   Promoter

   U6

   Available since

   Sept. 27, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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9. pCAG-TetR-RNF169UBD

   Plasmid

   #119011

   Purpose

   Mammalian expression of a fusion protein of single chain Tet repressor with the human RNF169 Ubiquitin binding domain and BFP expression

   Depositor

   Ralf Kuehn

   Article

   Bashir et al BMC Biotechnol. 2020 Oct 23;20(1):57. do

   Insert

   TetR
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CAG

   Available since

   Oct. 28, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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10. AP625-1

    Plasmid

    #70051

    Purpose

    Expresses myc::TEV::3Xflag::eGFP::3Xflag::TEV::myc in bacteria

    Depositor

    Geraldine Seydoux

    Article

    Paix et al Genetics. 2015 Sep;201(1):47-54. doi: 10

    Insert

    eGFP
    

    Use

    Tags

    3Xflag::TEV::myc and myc::TEV::3Xflag

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Oct. 22, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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11. EMX1_sgRNA

    Plasmid

    #100555

    Purpose

    Expresses EMX1 sgRNA. Target sequence: GAGTCCGAGCAGAAGAAGAA

    Depositor

    Dustin Maly

    Article

    Rose et al Nat Methods. 2017 Jul 24. doi: 10.1038/n

    Insert

    EMX1 sgRNA
    

    Use

    Tags

    Expression

    Mutation

    Promoter

    U6

    Available since

    Sept. 22, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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12. SK-YFP-TD-B

    Plasmid

    #48663

    Purpose

    Bacterial TD repression YFP reporter: protospacer B

    Depositor

    George Church

    Article

    Esvelt et al Nat Methods. 2013 Sep 29. doi: 10.1038/n

    Insert

    TD prototspacer B/PAM with reporter EYFP
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    lambda pR promoter

    Available since

    Oct. 17, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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13. SK-YFP-ST1-A

    Plasmid

    #48665

    Purpose

    Bacterial ST1 repression YFP reporter: protospacer A

    Depositor

    George Church

    Article

    Esvelt et al Nat Methods. 2013 Sep 29. doi: 10.1038/n

    Insert

    ST1 prototspacer A/PAM with reporter EYFP
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    lambda pR promoter

    Available since

    Oct. 17, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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14. PM-NM!TA

    Plasmid

    #48651

    Purpose

    Bacterial NM crRNA expression: targets NM to protospacer A (TACCATCTCAAGCTTGTTGA), p15A/chloramphenicol

    Depositor

    George Church

    Article

    Esvelt et al Nat Methods. 2013 Sep 29. doi: 10.1038/n

    Insert

    Bacterial NM crRNA to prototspacer A
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    J23100 promoter

    Available since

    Oct. 17, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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15. pmCherry-U6-SYNPO

    Plasmid

    #140587

    Purpose

    Expresses a gRNA and mCherry in mammalian cells

    Depositor

    Feng Zhang

    Article

    Schmid-Burgk et al Mol Cell. 2020 Mar 17. pii: S1097-2765(2

    Insert

    gRNA SYNPO
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available since

    May 21, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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16. MYC sgRNA4

    Plasmid

    #100556

    Purpose

    Expresses MYC sgRNA4. Target sequence: GTAATTCCAGCGAGAGGCAG

    Depositor

    Dustin Maly

    Article

    Rose et al Nat Methods. 2017 Jul 24. doi: 10.1038/n

    Insert

    MYC sgRNA4
    

    Use

    Tags

    Expression

    Mutation

    Promoter

    U6

    Available since

    Sept. 22, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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17. pAP608-1

    Plasmid

    #99484

    Purpose

    H2B::linker::GFP (contain 3 introns)::3XFlag::TEV::Myc::tbb-2 3UTR

    Depositor

    Geraldine Seydoux

    Article

    Paix et al Methods. 2017 May 15;121-122:86-93. doi:

    Insert

    H2B::linker::GFP with introns::3XFlag::TEV::Myc::tbb-2 3UTR
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Sept. 8, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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18. PM-TD!TA

    Plasmid

    #48655

    Purpose

    Bacterial TD crRNA expression: targets TD to protospacer A (TACCATCTCAAGCTTGTTGA), p15A/chloramphenicol

    Depositor

    George Church

    Article

    Esvelt et al Nat Methods. 2013 Sep 29. doi: 10.1038/n

    Insert

    Bacterial TD crRNA to prototspacer A
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    J23100 promoter

    Available since

    Oct. 17, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. PM-ST1!TB

    Plasmid

    #48654

    Purpose

    Bacterial ST1 crRNA expression: targets ST1 to protospacer B (ACTTTAAAAGTATTCGCCAT), p15A/chloramphenicol

    Depositor

    George Church

    Article

    Esvelt et al Nat Methods. 2013 Sep 29. doi: 10.1038/n

    Insert

    Bacterial ST1 crRNA to prototspacer B
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    J23100 promoter

    Available since

    Oct. 17, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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20. pDD401

    Plasmid

    #91828

    Purpose

    Pmyo-2::GFP FP-slot donor for the SapTrap cloning system

    Depositor

    Bob Goldstein

    Article

    Dickinson et al MicroPubl Biol. 2018 May 1;2018:10.17912

    Insert

    Pmyo-2::GFP
    

    Use

    CRISPR

    Tags

    Expression

    Worm

    Mutation

    Nucleotide 489 of the myo-2 promoter was changed …

    Promoter

    Available since

    June 23, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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