We narrowed to 8,512 results for: gnal

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pEGFP-C3-Sec10

Plasmid

#53759

Purpose

pEGFP-C3 plasmid that expresses Sec10

Depositor

Channing Der

Article

Martin et al Mol Cell. 2014 Jan 23;53(2):209-20. doi:

Insert

Sec10 (EXOC5 Human)

Tags

GFP

Expression

Mammalian

Available Since

Aug. 1, 2014

Availability

Academic Institutions and Nonprofits only
pENTR-myr-AKT-HA

Plasmid

#31790

Depositor

Xin Chen

Article

Calvisi et al Gastroenterology. 2011 Mar;140(3):1071-8

Insert

Akt1 (Akt1 Mouse)

Use

Entry vector

Tags

HA and Myr

Available Since

Oct. 21, 2011

Availability

Academic Institutions and Nonprofits only
HMGN1_pLENTI-CAG-IRES-GFP

Plasmid

#176995

Purpose

Mammalian lentiviral expression vector encoding HMGN1

Depositor

Roger Reeves

Article

Moyer et al Dis Model Mech. 2023 Apr 1;16(4):dmm0497

Insert

HMGN1 (HMGN1 Human)

Use

Lentiviral

Promoter

CAG

Available Since

April 19, 2022

Availability

Academic Institutions and Nonprofits only

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pCDNA3.1-Flag-H2A K5-9-118-119-125-127-129R

Plasmid

#63565

Purpose

Expression of human H2A (with multiple Lysine to Arginine point mutations) tagged with Flag

Depositor

Titia Sixma

Article

Mattiroli et al Cell. 2012 Sep 14;150(6):1182-95. doi: 1

Insert

H2A (H2AC20 Human)

Tags

Flag (MDYKDDDDKVPRIQ)

Expression

Mammalian

Mutation

Lysines at 5, 9, 118, 119, 125, 127, and 129 are…

Promoter

CMV

Available Since

March 13, 2015

Availability

Academic Institutions and Nonprofits only
vYM133_PB-pEF-osTIR1-T2A-iaaH-T2A-mGFPmut3-mAID-iCasp9-T2A-mCh-AID-BlastR

Plasmid

#160046

Purpose

For expressing the full paradoxical circuit

Depositor

Michael Elowitz

Article

Ma et al Cell. 2022 Mar 17;185(6):967-979.e12. do

Inserts

osTIR1
iaaH
iCasp9
BlastR

Use

Synthetic Biology

Tags

mAID, mCherry, and mGFPmut3

Expression

Mammalian

Promoter

no promoter, T2A is used and pEF1s

Available Since

April 11, 2022

Availability

Academic Institutions and Nonprofits only

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pUC19 EGFRup-CD3ε-mNeonGreen-EGFRdown

Plasmid

#188634

Purpose

HDR template for CD3ε-mNeonGreen knock-in at C-terminus of human EGFR

Depositor

Jared Toettcher

Article

Farahani et al eLife. 2023 May 22;12:e82863. doi: 10.75

Insert

EGFR (EGFR Synthetic, Human)

Use

CRISPR

Tags

CD3ε-mNeonGreen

Expression

Mammalian

Available Since

Aug. 16, 2022

Availability

Academic Institutions and Nonprofits only

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pcDNA3-Venus-cpVenus-FLARE-Cameleon

Plasmid

#123350

Purpose

Yellow-fluorescent homoFRET/anisotropy-based genetically encoded calcium indicator. Contains monomeric Venus/cpVenus-E172 homoFRET pair.

Depositor

Jin Zhang

Article

Ross et al eLife. 2018 Jul 3;7. pii: 35458. doi: 10

Insert

Venus-cpVenus-FLARE-Cameleon

Tags

6xHIS, T7 tag (gene 10 leader), Venus, Xpress (TM…

Expression

Mammalian

Promoter

CMV

Available Since

July 28, 2020

Availability

Academic Institutions and Nonprofits only

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pHR EGFR-SLP76-TagBFP

Plasmid

#188630

Purpose

Human EGFR labeled with SLP76 for monitoring with SLP76/VISH2 pYtag biosensor

Depositor

Jared Toettcher

Article

Farahani et al eLife. 2023 May 22;12:e82863. doi: 10.75

Insert

EGFR (EGFR Synthetic, Human)

Use

Lentiviral

Tags

SLP76-TagBFP

Expression

Mammalian

Promoter

SFFV

Available Since

Aug. 16, 2022

Availability

Academic Institutions and Nonprofits only

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pNL-CEF-IgA1-IRES-GFP

Plasmid

#187007

Purpose

Expression of human anti-SARS-CoV-2 S Protein Wuhan-Hu1 IgA1

Depositor

Hermann Eibel

Article

Sevdali et al Cell Rep. 2022 Jun 28;39(13):111019. doi

Insert

Human anti-SARS-CoV-2 S Protein Wuhan-Hu1 IgA1

Use

Lentiviral

Available Since

July 25, 2022

Availability

Academic Institutions and Nonprofits only

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mCherry-p62-TIR-AAA

Plasmid

#187901

Purpose

Mammalian cell expression of a triple mutant of p62: P228A, E230A, P232A

Depositor

Erika Holzbaur

Article

Harding et al Mol Cell. 2023 Sep 7;83(17):3188-3204.e7

Insert

SQSTM1 (SQSTM1 Human)

Tags

mCherry

Expression

Mammalian

Mutation

Triple alanine mutant: P228A, E230A, P232A

Promoter

CMV

Available Since

April 2, 2024

Availability

Academic Institutions and Nonprofits only

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pXL003-ishRNA-beta-catenin-2

Plasmid

#36299

Depositor

Sean Palecek

Article

Lian et al Proc Natl Acad Sci U S A. 2012 May 29.

Insert

shRNA of beta-catenin-2 (CTNNB1 Human)

Use

Lentiviral and RNAi

Tags

IRES-RFP-Puro

Expression

Mammalian

Promoter

TetO-H1

Available Since

Aug. 7, 2012

Availability

Academic Institutions and Nonprofits only
pcDNA3-Venus-cpVenus-FLARE-ICUE

Plasmid

#123355

Purpose

Yellow-fluorescent homoFRET/anisotropy-based cAMP indicator. Contains monomeric Venus/cpVenus-E172 homoFRET pair.

Depositor

Jin Zhang

Article

Ross et al eLife. 2018 Jul 3;7. pii: 35458. doi: 10

Insert

Venus-cpVenus-FLARE-ICUE

Tags

6xHIS, T7 tag (gene 10 leader), Venus, Xpress (TM…

Expression

Mammalian

Promoter

CMV

Available Since

Aug. 24, 2020

Availability

Academic Institutions and Nonprofits only

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FRB-GFP-Gab1(Y628F/Y660F)

Plasmid

#188658

Purpose

FRB coupled to GFP tagged Gab1 with Shp2 binding sites mutated to Phe

Depositor

Andrei Karginov

Article

Fauser et al J Cell Biol. 2022 Aug 1;221(8). pii: 213

Insert

GRB associated binding protein 1 (Gab1 Mouse)

Tags

FRB and GFP

Expression

Mammalian

Mutation

Y628F and Y660F

Promoter

CMV

Available Since

Aug. 19, 2022

Availability

Academic Institutions and Nonprofits only

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PCMV-intron myc Rab6 T27N

Plasmid

#46782

Purpose

expression of T27N Rab6a in mammalian cells

Depositor

Terry Hébert

Article

Dupre et al J Biol Chem. 2006 Nov 10;281(45):34561-7

Insert

Rab6 T27N (RAB6A Human)

Tags

myc

Expression

Mammalian

Mutation

T27N dominant negative

Promoter

CMV

Available Since

Nov. 14, 2013

Availability

Academic Institutions and Nonprofits only
pEF-Bos TRAM Flag

Plasmid

#41551

Depositor

Kate Fitzgerald, Doug Golenbock

Article

Fitzgerald et al J Exp Med. 2003 Oct 6;198(7):1043-55. Ep

Insert

TRAM (TICAM2 Human)

Tags

6xHis and Flag

Expression

Mammalian

Promoter

EF1a

Available Since

Dec. 14, 2012

Availability

Academic Institutions and Nonprofits only
3xNLS-NLP-cMyc-cMyc LbaCas12a

Plasmid

#182125

Purpose

pET21a protein expression vector for 3xNLS-NLP-cMyc-cMyc LbaCas12a in bacteria

Depositor

Scot Wolfe

Article

Luk et al GEN Biotechnol. 2022 Jun;1(3):271-284. d

Insert

3xNLS-NLP-cMyc-cMyc LbaCas12a

Tags

6xHis

Expression

Bacterial

Promoter

T7

Available Since

Sept. 30, 2022

Availability

Academic Institutions and Nonprofits only

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mChF-AIP

Plasmid

#61527

Purpose

Expression of human FK506 binding protein 1A (FKBP1A) tagged with mCherry and AIP

Depositor

Takanari Inoue

Article

Miyamoto et al Cell Rep. 2015 Apr 28;11(4):657-70. doi:

Insert

FK506 binding protein 1A (FKBP1A Human)

Tags

AIP and mCherry

Expression

Mammalian

Promoter

CMV

Available Since

April 17, 2015

Availability

Academic Institutions and Nonprofits only
MmEos3.2

Plasmid

#203754

Purpose

Encodes the membrane anchor of Src including the first 15 resideues of the N terminus, with 1 myristoylation and short polybasic sequence, with mEos3.2 fused to the C terminus. Used as a fluorescent plasma membrane probe.

Depositor

Sarah Veatch

Article

Shelby et al Nat Chem Biol. 2023 Jun;19(6):750-758. d

Insert

Src15 n-term (SRC Human)

Tags

mEos3.2

Expression

Mammalian

Available Since

Aug. 7, 2023

Availability

Academic Institutions and Nonprofits only

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pcDNA3-Venus-cpVenus-FLARE-AKAR

Plasmid

#123329

Purpose

Yellow-fluorescent homoFRET/anisotropy-based biosensor for monitoring Protein Kinase A activity. Contains monomeric Venus/cpVenus-E172 homoFRET pair.

Depositor

Jin Zhang

Article

Ross et al eLife. 2018 Jul 3;7. pii: 35458. doi: 10

Insert

Venus-cpVenus-FLARE-AKAR

Tags

6xHIS, T7 tag (gene 10 leader), Venus, Xpress (TM…

Expression

Mammalian

Promoter

CMV

Available Since

Sept. 29, 2020

Availability

Academic Institutions and Nonprofits only

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pHtrA-TEV-His12

Plasmid

#137036

Purpose

expression clone from T7 promoter for full-length (signal peptide containing) B. burgdorferi HtrA with C-terminal TEV-protease-cleavable His12

Depositor

Debra Hansen

Article

Robertson et al Sci Rep. 2019 Nov 26;9(1):17606. doi: 10

Insert

AAC66500.2 (BB_0104 Borrelia burgdorferi B31)

Tags

His12 (TEV protease cleavable)

Expression

Bacterial

Mutation

wild type, full-length protein, contains signal p…

Promoter

T7

Available Since

March 23, 2020

Availability

Academic Institutions and Nonprofits only

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