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pEJS1964 All-in-one AAV-U1a-NmeABE-8e-2xBPSV40-miniU6-Rosa26

Plasmid

#199262

Purpose

Single AAV vector for expressing N-terminal fusion Nme2Cas9-ABE8e and one miniU6 driven sgRNA targeting mouse Rosa26 gene

Depositor

Erik Sontheimer

Article

Zhang et al GEN Biotechnol. 2022 Jun 1;1(3):285-299.

Insert

NmeABE8e

Use

AAV and CRISPR

Tags

NLS

Expression

Mammalian

Promoter

U1a

Available Since

April 10, 2023

Availability

Academic Institutions and Nonprofits only

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pAAV-DIO-NS1

Plasmid

#175273

Purpose

AAV vector mediating Cre-depenent expression of NS1 gene of YFV-17D

Depositor

Wei Xu

Article

Li et al Nat Methods. 2021 Nov 25. pii: 10.1038/s

Insert

Nonstructural protein 1 (NS1) of YFV-17D (POLY Yellow fever virus strain 17D)

Use

AAV

Promoter

Synapsin

Available Since

Nov. 30, 2021

Availability

Academic Institutions and Nonprofits only

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pAAV-DIO-NS1

Plasmid

#175273

Purpose

AAV vector mediating Cre-depenent expression of NS1 gene of YFV-17D

Depositor

Wei Xu

Article

Li et al Nat Methods. 2021 Nov 25. pii: 10.1038/s

Insert

Nonstructural protein 1 (NS1) of YFV-17D (POLY Yellow fever virus strain 17D)

Use

AAV

Promoter

Synapsin

Available Since

Nov. 30, 2021

Availability

Academic Institutions and Nonprofits only

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pAGT9569

Plasmid

#219431

Purpose

Transcriptional unit (MoClo-position 2): pAtRPS5a_PapE-4LF2-N-SpCas9i-N_tNOS

Depositor

Tom Schreiber

Article

Schreiber et al Mol Plant. 2024 Mar 22:S1674-2052(24)000

Insert

LB_AtRPS5a:PapE-4xLF2-NLS-SpCas9i-NLS:tNOS_RB (Position 2)

Use

CRISPR and Synthetic Biology; Moclo compatible le…

Tags

PapE-4LF2

Expression

Plant

Available Since

July 3, 2024

Availability

Academic Institutions and Nonprofits only

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pAGT9569

Plasmid

#219431

Purpose

Transcriptional unit (MoClo-position 2): pAtRPS5a_PapE-4LF2-N-SpCas9i-N_tNOS

Depositor

Tom Schreiber

Article

Schreiber et al Mol Plant. 2024 Mar 22:S1674-2052(24)000

Insert

LB_AtRPS5a:PapE-4xLF2-NLS-SpCas9i-NLS:tNOS_RB (Position 2)

Use

CRISPR and Synthetic Biology; Moclo compatible le…

Tags

PapE-4LF2

Expression

Plant

Available Since

July 3, 2024

Availability

Academic Institutions and Nonprofits only

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NSP4-WT-Flag

Plasmid

#210339

Purpose

Expresses SARS-CoV-2 NSP4 fused with Flag in mammalian cells

Depositor

Michele Pagano

Article

Li et al J Virol. 2023 Sep 28:e0050723. doi: 10.1

Insert

SARS-CoV-2 NSP4 (ORF1ab Human, SARS-CoV-2 isolate Wuhan-Hu-1)

Tags

Flag

Expression

Mammalian

Mutation

Many synonymous changes due to codon optimization

Available Since

Jan. 3, 2024

Availability

Academic Institutions and Nonprofits only

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NSP4-WT-Flag

Plasmid

#210339

Purpose

Expresses SARS-CoV-2 NSP4 fused with Flag in mammalian cells

Depositor

Michele Pagano

Article

Li et al J Virol. 2023 Sep 28:e0050723. doi: 10.1

Insert

SARS-CoV-2 NSP4 (ORF1ab Human, SARS-CoV-2 isolate Wuhan-Hu-1)

Tags

Flag

Expression

Mammalian

Mutation

Many synonymous changes due to codon optimization

Available Since

Jan. 3, 2024

Availability

Academic Institutions and Nonprofits only

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TRE-mCardinal-P2A-B19L Flp-in vector

Plasmid

#178519

Purpose

For making TRE-CB mouse line in Tigre locus

Depositor

Ian Wickersham

Article

Jin et al Nat Neurosci. 2024 Jan 11. doi: 10.1038/

Insert

mCardinal-P2A-B19L

Expression

Mammalian

Available Since

Jan. 24, 2024

Availability

Academic Institutions and Nonprofits only

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TRE-mCardinal-P2A-B19L Flp-in vector

Plasmid

#178519

Purpose

For making TRE-CB mouse line in Tigre locus

Depositor

Ian Wickersham

Article

Jin et al Nat Neurosci. 2024 Jan 11. doi: 10.1038/

Insert

mCardinal-P2A-B19L

Expression

Mammalian

Available Since

Jan. 24, 2024

Availability

Academic Institutions and Nonprofits only

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Cbh_v5 AAV-saCBE C-terminal

Plasmid

#137183

Purpose

AAV genome: expresses the C-terminal of S. aureus v5 AAV-CBE from the Cbh promoter, and U6-sgRNA

Depositor

David Liu

Article

Levy et al Nat Biomed Eng. 2020 Jan;4(1):97-110. do

Insert

v5 AAV-saCBE C-terminal

Use

AAV

Promoter

Cbh

Available Since

Jan. 31, 2020

Availability

Academic Institutions and Nonprofits only

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Cbh_v5 AAV-saCBE C-terminal

Plasmid

#137183

Purpose

AAV genome: expresses the C-terminal of S. aureus v5 AAV-CBE from the Cbh promoter, and U6-sgRNA

Depositor

David Liu

Article

Levy et al Nat Biomed Eng. 2020 Jan;4(1):97-110. do

Insert

v5 AAV-saCBE C-terminal

Use

AAV

Promoter

Cbh

Available Since

Jan. 31, 2020

Availability

Academic Institutions and Nonprofits only

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pcDNA-Ikka-HA (K44M)

Plasmid

#23297

Depositor

Warner Greene

Article

Geleziunas et al Mol Cell Biol. 1998 Sep . 18(9):5157-65.

Insert

IKK alpha (Chuk Mouse)

Tags

HA

Expression

Mammalian

Mutation

K44M

Available Since

April 12, 2010

Availability

Academic Institutions and Nonprofits only
pcDNA-Ikka-HA (K44M)

Plasmid

#23297

Depositor

Warner Greene

Article

Geleziunas et al Mol Cell Biol. 1998 Sep . 18(9):5157-65.

Insert

IKK alpha (Chuk Mouse)

Tags

HA

Expression

Mammalian

Mutation

K44M

Available Since

April 12, 2010

Availability

Academic Institutions and Nonprofits only
3019_pETcon-SARS-CoV-2-RBD_E484K

Plasmid

#184404

Purpose

yeast surface display of the SARS-CoV-2 Eta variant RBD

Depositor

Jesse Bloom

Article

Starr et al Science. 2022 Jun 28:eabo7896. doi: 10.1

Insert

SARS-CoV-2 Eta Spike receptor binding domain (RBD) (S SARS-CoV-2 virus)

Tags

HA and c-Myc

Expression

Yeast

Mutation

E484K

Available Since

May 23, 2022

Availability

Academic Institutions and Nonprofits only

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3019_pETcon-SARS-CoV-2-RBD_E484K

Plasmid

#184404

Purpose

yeast surface display of the SARS-CoV-2 Eta variant RBD

Depositor

Jesse Bloom

Article

Starr et al Science. 2022 Jun 28:eabo7896. doi: 10.1

Insert

SARS-CoV-2 Eta Spike receptor binding domain (RBD) (S SARS-CoV-2 virus)

Tags

HA and c-Myc

Expression

Yeast

Mutation

E484K

Available Since

May 23, 2022

Availability

Academic Institutions and Nonprofits only

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pEJS-HZ54 All-in-one AAV-U1a-NmeABE8e-2xBPSV40-U6-Rosa26

Plasmid

#199265

Purpose

Single AAV vector for expressing N-terminal fusion Nme2Cas9-ABE8e and one U6 driven sgRNA targeting mouse Rosa26 gene

Depositor

Erik Sontheimer

Article

Zhang et al GEN Biotechnol. 2022 Jun 1;1(3):285-299.

Insert

NmeABE8e

Use

AAV and CRISPR

Tags

NLS

Expression

Mammalian

Promoter

U1a

Available Since

April 10, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pEJS-HZ54 All-in-one AAV-U1a-NmeABE8e-2xBPSV40-U6-Rosa26

Plasmid

#199265

Purpose

Single AAV vector for expressing N-terminal fusion Nme2Cas9-ABE8e and one U6 driven sgRNA targeting mouse Rosa26 gene

Depositor

Erik Sontheimer

Article

Zhang et al GEN Biotechnol. 2022 Jun 1;1(3):285-299.

Insert

NmeABE8e

Use

AAV and CRISPR

Tags

NLS

Expression

Mammalian

Promoter

U1a

Available Since

April 10, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pcDNA3.1_GP1Fc Cl13

Plasmid

#138599

Purpose

Expresses GP1 of LCMV Cl13 fused with human IgG (Fc fusion)

Depositor

Annette Oxenius

Article

Krautler et al Cell Rep. 2020 Jan 28;30(4):997-1012.e6.

Insert

LCMV GP1-Fc

Tags

Fc fusion protein

Expression

Mammalian

Promoter

CMV

Available Since

April 30, 2020

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1_GP1Fc Cl13

Plasmid

#138599

Purpose

Expresses GP1 of LCMV Cl13 fused with human IgG (Fc fusion)

Depositor

Annette Oxenius

Article

Krautler et al Cell Rep. 2020 Jan 28;30(4):997-1012.e6.

Insert

LCMV GP1-Fc

Tags

Fc fusion protein

Expression

Mammalian

Promoter

CMV

Available Since

April 30, 2020

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-MCS-13 x Linker-BioID2-HA-GPI-PSS

Plasmid

#194750

Purpose

To fuse your protein of interest to the N-terminus of GPI-anchored-BioID2

Depositor

Chaoyang Li

Article

Gao et al iScience. 2022 Nov 2;25(12):105481. doi:

Type

Empty backbone

Tags

HA

Expression

Mammalian

Available Since

April 20, 2023

Availability

Academic Institutions and Nonprofits only

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