We narrowed to 4,976 results for: puc

1. pUC19, containing H2-Kd Balb/c WK10

   Plasmid

   #102642

   Purpose

   Encodes sequence of the murine H2-Kd allele

   Depositor

   Sai Reddy

   Article

   Kelton et al Sci Rep. 2017 Apr 4;7:45775. doi: 10.103

   Insert

   H2-Kd Balb/c
   

   Use

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Jan. 2, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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2. pUC19-link-mCherry-FLAG:cat

   Plasmid

   #211831

   Purpose

   Plasmid template that encodes the fluorescent protein mCherry with a FLAG tag next to chloramphenicol resistence gene. This template was used to copy out mcherry-FLAG-cat for genetic tagging.

   Depositor

   Peng Chen

   Insert

   mCherry-FLAG-cat
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   NA

   Promoter

   Available since

   Jan. 18, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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3. pUC19-link-PAGFP-FLAG:Kan

   Plasmid

   #211834

   Purpose

   Plasmid template that encodes the fluorescent protein PAGFP with a FLAG tag next to kanamycin resistance gene. This template was used to copy out PAGFP-FLAG-Kan for genetic tagging.

   Depositor

   Peng Chen

   Insert

   PAGFP-FLAG-Kan
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   NA

   Promoter

   Available since

   Jan. 18, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pUC-UAS1B16-TEF(203)

   Plasmid

   #44377

   Depositor

   Hal Alper

   Article

   Blazeck et al Appl Environ Microbiol. 2011 Nov;77(22):

   Insert

   UAS1B16-TEF(203) promoter
   

   Use

   Synthetic Biology

   Tags

   Expression

   Bacterial

   Mutation

   N/A

   Promoter

   UAS1B16-TEF(203)

   Available since

   April 16, 2013

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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5. pUC19-TSV (linearized in VP2)

   Plasmid

   #70032

   Purpose

   pUC19-TSV (linearized in VP2) contains a genome of the Trichodysplasia spinulosa-associated polyomaviruse (GenBank NC_014361) linearized and cloned in the VP2-coding region

   Depositor

   Mariet Feltkamp

   Article

   van der Meijden et al PLoS Pathog. 2010 Jul 29;6(7):e1001024.

   Insert

   TSV full genome, linearized in VP2 gene
   

   Use

   Cloning vector

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Dec. 15, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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6. pUC57-GSX2-tTA-BleoR

   Plasmid

   #161748

   Purpose

   Tet-ON tTA plasmid for the c-terminal targeting of human GSX2 locus

   Depositor

   Elena Cattaneo

   Article

   Besusso et al Stem Cell Res. 2020 Dec;49:102016. doi:

   Insert

   Advanced tTA
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   endogenous GSX2 promoter

   Available since

   Dec. 11, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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7. pCfB1975 (pUC19-PTEF1-PPGK1)

   Plasmid

   #73296

   Purpose

   Example plasmid containing a bidirectional promoter

   Depositor

   Irina Borodina

   Article

   Jessop-Fabre et al Biotechnol J. 2016 Aug;11(8):1110-7. doi

   Insert

   pTEF1-pPGK1
   

   Use

   Synthetic Biology

   Tags

   Expression

   Bacterial and Yeast

   Mutation

   Promoter

   Available since

   Nov. 23, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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8. pUC_h7SK Mma PylT (CUA)

   Plasmid

   #154782

   Purpose

   plasmid with single copy of Mma PylT (CUA anticodon)

   Depositor

   Simon Elsaesser

   Article

   Meineke et al ACS Chem Biol. 2018 Sep 27. doi: 10.1021

   Insert

   Mma PylT
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   U25C

   Promoter

   h7SK

   Available since

   Oct. 1, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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9. pUC CBA-SpCas9N863Anickase.EF1a-BFP.sgLMNApair1

   Plasmid

   #98976

   Purpose

   Cas9 Homologous Recombination Reporter. SpCas9N863A nickase and a pair of sgRNAs targeting hLMNA. Generates breaks with 44bp 3' overhangs. TagBFP.

   Depositor

   Jan Karlseder

   Article

   Arnoult et al Nature. 2017 Sep 20;549(7673):548-552. d

   Insert

   LMNA sgRNAs pair 1 and Cas9(N863A)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Nov. 29, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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10. pUC19 ACMV DNA-B 1.5mer

    Plasmid

    #159135

    Purpose

    Infectious clone containing a partial-tandem-dimer of African cassava mosaic virus CM:2:98 DNA-B

    Depositor

    Vincent Fondong

    Article

    Hoyer et al Microbiol Resour Announc. 2020 Nov 12;9(

    Insert

    ACMV DNA-B 1.5mer
    

    Use

    Infectious clone for bombarding plants

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Nov. 30, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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11. pUC19-Top-Toehold-mCherry

    Plasmid

    #87318

    Purpose

    Expresses the "Top" transcript of the Split-Broccoli system upstream of an RNA Toehold switch controlling mCherry.

    Depositor

    Donald Burke

    Article

    Alam et al ACS Synth Biol. 2017 May 26. doi: 10.102

    Insert

    Split-Broccoli "Top" transcript upstream of an RNA Toehold (TS2_KS01) controlling mCherry translation.
    

    Use

    Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    P70a

    Available since

    May 23, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pUC-UAS1B16-TEF(1004)

    Plasmid

    #44365

    Depositor

    Hal Alper

    Article

    Blazeck et al Appl Environ Microbiol. 2011 Nov;77(22):

    Insert

    UAS1B16-TEF(1004) promoter
    

    Use

    Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    N/A

    Promoter

    UAS1B16-TEF(1004)

    Available since

    July 15, 2013

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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13. pUC_h7SK Mma PylT (UUA)

    Plasmid

    #154783

    Purpose

    plasmid with single copy of Mma PylT (UUA anticodon)

    Depositor

    Simon Elsaesser

    Article

    Meineke et al Cell Rep. 2020 Jun 23;31(12):107811. doi

    Insert

    Mma PylT
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    U25C and UUA anticodon

    Promoter

    h7SK

    Available since

    Sept. 16, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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14. pUC57-Sa sgRNA expression vector

    Plasmid

    #107720

    Purpose

    For in vitro transcription of Sa sgRNA from the T7 promoter.

    Depositor

    Xingxu Huang

    Article

    Liu et al Nat Commun. 2018 Jun 14;9(1):2338. doi:

    Type

    Empty backbone

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available since

    April 18, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pUC19-P70a-Top-T~Bottom-T

    Plasmid

    #87315

    Purpose

    Control plasmid. Encodes the Split-Broccoli system (Top + Bottom) with only "Top" under the control of E. coli sigma 70 promoter.

    Depositor

    Donald Burke

    Article

    Alam et al ACS Synth Biol. 2017 May 26. doi: 10.102

    Insert

    Split-Broccoli system (promoter control)
    

    Use

    Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    P70a

    Available since

    May 23, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pUC19 gene 60 delta gap

    Plasmid

    #49091

    Purpose

    Template for generating gene 60 mRNA, coding gap deletion

    Depositor

    Nils Walter

    Article

    Todd et al RNA. 2013 May;19(5):685-700. doi: 10.126

    Insert

    Bacteriophage T4 gene 60
    

    Use

    Tags

    Expression

    Mutation

    Coding gap and coding gap STOP have been deleted.

    Promoter

    T7

    Available since

    Jan. 23, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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17. pUC18-mini-Tn7T-Gm-LacZ30

    Plasmid

    #65028

    Purpose

    GmR mini-Tn7 vector, for construction of B-galactosidase protein fusions, frame 3. See Depositor Comments below for information regarding the lacZ sequence.

    Depositor

    Herbert Schweizer

    Article

    Choi et al Nat Protoc. 2006;1(1):153-61.

    Type

    Empty backbone

    Use

    Tags

    lacZ

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Nov. 2, 2015

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

18. pUC 3GLA UAS HAi

    Plasmid

    #71763

    Purpose

    gap-repair recombineering for Drosophila transgenesis, contains loxP cassette with GFP, Amp resistant, with 5xUAS and HA tag

    Depositor

    Matthias Soller

    Article

    Haussmann et al Nucleic Acids Res. 2018 Dec 12. pii: 523

    Type

    Empty backbone

    Use

    Tags

    Expression

    Insect

    Mutation

    Promoter

    Available since

    Dec. 5, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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19. pUC-UAS1B8-TEF(1004)

    Plasmid

    #44366

    Depositor

    Hal Alper

    Article

    Blazeck et al Appl Environ Microbiol. 2011 Nov;77(22):

    Insert

    UAS1B8-TEF(1004) promoter
    

    Use

    Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    N/A

    Promoter

    UAS1B8-TEF(1004)

    Available since

    July 15, 2013

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

20. Mishler2014-No-aptamer-controlluciferase-pUC19

    Plasmid

    #63856

    Purpose

    This pUC19 vector contains a no-aptamer control mRNA encoding luciferase gene. It should be used when testing AEB-TMR7 and AEB-TMR10 riboswitches (all on pUC19) to measure their non-specific ligand effect on luciferase expression.

    Depositor

    Howard Salis

    Article

    Espah Borujeni et al Nucleic Acids Res. 2015 Nov 30. pii: gkv

    Insert

    No-aptamer control mRNA
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    tac

    Available since

    Feb. 19, 2016

    Availability

    Academic Institutions and Nonprofits only

21. pUC-UAS1B8-TEF(203)

    Plasmid

    #44378

    Depositor

    Hal Alper

    Article

    Blazeck et al Appl Environ Microbiol. 2011 Nov;77(22):

    Insert

    UAS1B8-TEF(203) promoter
    

    Use

    Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    N/A

    Promoter

    UAS1B8-TEF(203)

    Available since

    April 18, 2013

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

22. pUC-UAS1B8-TEF(804)

    Plasmid

    #44368

    Depositor

    Hal Alper

    Article

    Blazeck et al Appl Environ Microbiol. 2011 Nov;77(22):

    Insert

    UAS1B8-TEF(804) promoter
    

    Use

    Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    N/A

    Promoter

    UAS1B8-TEF(804)

    Available since

    April 18, 2013

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

23. pUC57-FlpTag-GFP-ph0

    Plasmid

    #231897

    Purpose

    Vector with FlpTag-GFP, an inverted GFP enabling conditional tagging and visualization of endogenous proteins via Flp recombinase activity. Codon phase 0.

    Depositor

    Alexander Borst

    Article

    Fendl et al Elife. 2020 Oct 20;9:e62953. doi: 10.755

    Insert

    SD-GFP-SA
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    June 23, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pUC57-FlpTag-GFP-ph1

    Plasmid

    #231898

    Purpose

    Vector with FlpTag-GFP, an inverted GFP enabling conditional tagging and visualization of endogenous proteins via Flp recombinase activity. Codon phase 1.

    Depositor

    Alexander Borst

    Article

    Fendl et al Elife. 2020 Oct 20;9:e62953. doi: 10.755

    Insert

    SD-GFP-SA
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    June 23, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pUC57-FlpTag-GFP-ph2

    Plasmid

    #231899

    Purpose

    Vector with FlpTag-GFP, an inverted GFP enabling conditional tagging and visualization of endogenous proteins via Flp recombinase activity. Codon phase 2.

    Depositor

    Alexander Borst

    Article

    Fendl et al Elife. 2020 Oct 20;9:e62953. doi: 10.755

    Insert

    SD-GFP-SA
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    June 23, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pUC19_msfGFP-HP1a-repair-template

    Plasmid

    #238248

    Purpose

    For knock-in msfGFP to HP1a locus

    Depositor

    Denes Hnisz

    Insert

    HP1a
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available since

    June 3, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pUC19_msfGFP-EWSR1-repair-tempate

    Plasmid

    #237682

    Purpose

    For knock-in msfGFP to EWSR1 locus

    Depositor

    Denes Hnisz

    Insert

    EWSR1 (EWSR1 Human)
    

    Use

    CRISPR

    Tags

    msfGFP

    Expression

    Mutation

    Promoter

    Available since

    May 6, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pUC19_msfGFP-TCOF1-repair-template

    Plasmid

    #237632

    Purpose

    For knock-in msfGFP to TCOF1 locus

    Depositor

    Denes Hnisz

    Insert

    TCOF1 (TCOF1 Human)
    

    Use

    CRISPR

    Tags

    msfGFP

    Expression

    Mutation

    Promoter

    Available since

    May 6, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pUC19_msfGFP-FUS-repair-tempate

    Plasmid

    #237684

    Purpose

    For knock-in msfGFP to FUS locus

    Depositor

    Denes Hnisz

    Insert

    FUS (FUS Human)
    

    Use

    CRISPR

    Tags

    msfGFP

    Expression

    Mutation

    Promoter

    Available since

    May 6, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pUC19::ZmUBI-RWT4Ca+C-Tail

    Plasmid

    #237514

    Purpose

    Protoplast expression

    Depositor

    Gitta Coaker

    Article

    Sung et al Nat Genet. 2025 Apr 14. doi: 10.1038/s41

    Insert

    Rwt4Ca+C-Tail
    

    Use

    Tags

    Expression

    Plant

    Mutation

    Add C-terminal extension

    Promoter

    Available since

    April 28, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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