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Showing: 1251 - 1300 of 9524 results
  1. pTU1-D-rrnOD

    Plasmid
    #112786
    Purpose
    Downstream homology arm, rrnO-23S (23S rRNA)
    Depositor
    Insert
    rrnOD
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    Aug. 14, 2018
    Availability
    Academic Institutions and Nonprofits only
  2. pTU1-D-amyED

    Plasmid
    #112782
    Purpose
    Downstream homology arm, amyE encodes _-amylase
    Depositor
    Insert
    amyED
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    Aug. 14, 2018
    Availability
    Academic Institutions and Nonprofits only
  3. pTU1-A-amyEU

    Plasmid
    #112781
    Purpose
    Upstream homology arm, amyE encodes _-amylase
    Depositor
    Insert
    amyEU
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    Aug. 14, 2018
    Availability
    Academic Institutions and Nonprofits only
  4. pJK691

    Plasmid
    #72381
    Purpose
    Produces Escherichia coli BolA, N-terminal His6 tag (H6EcBolA)
    Depositor
    Insert
    stationary-phase morphogene, transcriptional repressor for mreB; also regulator for dacA, dacC, and ampC (bolA Escherichia coli str. K-12 substr. MG1655)
    Use
    Tags
    His6
    Expression
    Bacterial
    Mutation
    Promoter
    T7
    Available since
    March 4, 2016
    Availability
    Academic Institutions and Nonprofits only
  5. Flag-neo-PP1-Gamma

    Plasmid
    #171270
    Purpose
    Mammalian expression of 3x-Flag-tagged human PP1 gamma catalytic subunit isoform 1
    Depositor
    Insert
    3x-Flag-tagged human PP1 gamma catalytic subunit isoform 1 (PPP1CC Human)
    Use
    Tags
    Flag
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    June 30, 2021
    Availability
    Academic Institutions and Nonprofits only
  6. pJK642

    Plasmid
    #72391
    Purpose
    Produces Escherichia coli glutamine synthetase, N-terminal His6 tag and Y397F mutation that prevents adenylation (H6EcGlnA-Y397F)
    Depositor
    Insert
    glutamine synthetase (glnA Escherichia coli str. K-12 substr. MG1655))
    Use
    Tags
    His6
    Expression
    Bacterial
    Mutation
    Mutated tyrosine-397 to phenylalanine to block ad…
    Promoter
    T7
    Available since
    March 4, 2016
    Availability
    Academic Institutions and Nonprofits only
  7. pDS-Red1-N1 AMPA GluR-B(flip)

    Plasmid
    #109142
    Purpose
    The expression vector carries the red fluorescent protein tagged at the C-terminus of GluA2 subunit flip spliced form of the AMPA receptor.
    Depositor
    Insert
    AMPA receptor
    Use
    Tags
    RFP
    Expression
    Mammalian
    Mutation
    Glua2 subunit flip spliced form of the AMPA recep…
    Promoter
    CMV
    Available since
    June 15, 2018
    Availability
    Academic Institutions and Nonprofits only
  8. pJK650

    Plasmid
    #72400
    Purpose
    Produces Escherichia coli glutamine synthetase with Y397F mutation that prevents adenylation (EcGlnA-Y397F)
    Depositor
    Insert
    glutamine synthetase (glnA Escherichia coli str. K-12 substr. MG1655))
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Mutated tyrosine-397 to phenylalanine to block ad…
    Promoter
    T7
    Available since
    March 21, 2016
    Availability
    Academic Institutions and Nonprofits only
  9. pTU1-D-recJD

    Plasmid
    #112784
    Purpose
    Downstream homology arm, RecJ is a ssDNA specific exonuclease, involved in genome repair and maintenance
    Depositor
    Insert
    recJD
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    Aug. 14, 2018
    Availability
    Academic Institutions and Nonprofits only
  10. His8:MBP-tev-Gly-eK-Flv

    Plasmid
    #83383
    Purpose
    bacterial expression vector pVP16 containing a derivative of E. coli lacZ fused with E. coli flavin binding protein MioC to be used in N-end rule in vitro ubiquitination studies
    Depositor
    Insert
    flavin binding protein MioC
    Use
    Synthetic Biology
    Tags
    3xHA, 8xHis, and MBP
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    April 21, 2017
    Availability
    Academic Institutions and Nonprofits only
  11. pD(P+T)_phN

    Plasmid
    #223858
    Purpose
    4G-cloning donor for insect promoter and terminator, N-terminal tag added
    Depositor
    Insert
    polyhedrin promoter and SV40 polyA
    Use
    Golden-gate donor
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Sept. 19, 2024
    Availability
    Academic Institutions and Nonprofits only
  12. pD(P+T)_phC

    Plasmid
    #223859
    Purpose
    4G-cloning donor for insect promoter and terminator, C-terminal tag added
    Depositor
    Insert
    polyhedrin promoter and SV40 polyA
    Use
    Golden-gate donor
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Sept. 19, 2024
    Availability
    Academic Institutions and Nonprofits only
  13. pIND4-RC-C2-MmXYRS

    Plasmid
    #222744
    Purpose
    Expresses 3 subunits of photosynthetic reaction center from R. sphaeroides and aaRS-tRNA pair for incorporating haloalkane crosslinker
    Depositor
    Inserts
    puhA
    pufL
    pufM
    MmXYRS
    Mm Pyl tRNA
    Use
    Tags
    6xHis
    Expression
    Mutation
    Contains the following substitutions: V346A, W348…
    Promoter
    rrnB
    Available since
    Sept. 11, 2024
    Availability
    Academic Institutions and Nonprofits only
  14. pB2HoptX

    Plasmid
    #163832
    Purpose
    Inactive two-hybrid system that confers spectinomycin resistance in response to substrate phosphorylation. Constitutive expression of c-src kinase, CDC37, and PTP1B
    Depositor
    Insert
    SpecR
    Use
    Synthetic Biology
    Tags
    Expression
    Bacterial
    Mutation
    MidT Substrate Y4F
    Promoter
    Pro1;ProD;pLacZ-Opt
    Available since
    July 14, 2021
    Availability
    Academic Institutions and Nonprofits only
  15. pCWXPGR-pTF-EpoRtm

    Plasmid
    #114286
    Purpose
    TET-inducible expression of Erythropoietin receptor without cytoplasmic tail
    Depositor
    Insert
    Erythropoietin Receptor (Epor Rat)
    Use
    Lentiviral
    Tags
    Expression
    Mutation
    deletion of the cytoplasmic domain
    Promoter
    Available since
    Sept. 11, 2018
    Availability
    Academic Institutions and Nonprofits only
  16. pTU1-A-recJU

    Plasmid
    #112783
    Purpose
    Upstream homology arm, RecJ is a ssDNA specifiC exonuclease, involved in genome repair and maintenance
    Depositor
    Insert
    recJU
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    Aug. 15, 2018
    Availability
    Academic Institutions and Nonprofits only
  17. pBP-PliaG

    Plasmid
    #112777
    Purpose
    Weak constitutive promoter of liaGFSR operon involved in detection of cell wall damage
    Depositor
    Insert
    PliaG promoter
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    Aug. 14, 2018
    Availability
    Academic Institutions and Nonprofits only
  18. His8:MBP-tev-Trp-eK-Flv

    Plasmid
    #83390
    Purpose
    bacterial expression vector pVP16 containing a derivative of E. coli lacZ fused with E. coli flavin binding protein MioC to be used in N-end rule in vitro ubiquitination studies
    Depositor
    Insert
    flavin binding protein MioC
    Use
    Synthetic Biology
    Tags
    3xHA, 8xHis, and MBP
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    April 21, 2017
    Availability
    Academic Institutions and Nonprofits only
Showing: 1251 - 1300 of 9524 results