We narrowed to 1,917 results for: FRT

1. pDEST-FRT/TO-3xFLAG-REV7

   Plasmid

   #114127

   Purpose

   Expresses N-terminally tagged 3x FLAG-REV7 in mammalian cells

   Depositor

   Daniel Durocher

   Article

   Noordermeer et al Nature. 2018 Aug;560(7716):117-121. doi:

   Insert

   REV7 (MAD2L2 Human)
   

   Use

   Genomic integration, tetracycline inducible

   Tags

   3xFLAG

   Expression

   Mammalian

   Mutation

   Promoter

   CMV/TetO2

   Available Since

   Aug. 16, 2018

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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2. pCDNA5/FRT/TO_TOM20-HaloTag7_T2A_mEGFP

   Plasmid

   #187072

   Purpose

   Mitochondria surface expression of HaloTag7 in mammalian cells

   Depositor

   Kai Johnsson

   Article

   Kompa et al J Am Chem Soc. 2023 Feb 8;145(5):3075-30

   Insert

   TOM20-HaloTag7
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   Aug. 5, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pcDNA5/FRT/TO-GAlphasS-RLuc8

   Plasmid

   #140980

   Purpose

   Encodes a G alpha subunit (GNAS2) containing RLuc8 as an optimal component of a BRET2 biosensor for studying heterotrimeric G protein dissociation

   Depositor

   Bryan Roth

   Article

   Olsen et al Nat Chem Biol. 2020 May 4. pii: 10.1038/

   Insert

   GAlphasS-RLuc8
   

   Use

   Luciferase

   Tags

   Expression

   Mammalian

   Mutation

   RLuc8 and flanking SGGGGS linkers have been inser…

   Promoter

   CMV

   Available Since

   June 26, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pEF5B-FRT-GFP-αTAT1

   Plasmid

   #27099

   Depositor

   Maxence Nachury

   Article

   Shida et al Proc Natl Acad Sci U S A. 2010 Dec 14;10

   Insert

   α-Tubulin K40 acetyltransferase1 (ATAT1 Human)
   

   Use

   Tags

   GFP, S-tag, and TEV site

   Expression

   Mammalian

   Mutation

   Promoter

   EF1a

   Available Since

   Jan. 5, 2011

   Availability

   Academic Institutions and Nonprofits only

   

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5. pAAV-FLEXfrt-EGFP-KASH

   Plasmid

   #154374

   Purpose

   Vector for Flp-dependent expression of EGFP tagged KASH protein domain

   Depositor

   Larry Zweifel

   Article

   Hunker et al Cell Rep. 2020 Mar 24;30(12):4303-4316.e

   Insert

   EGFP-KASH
   

   Use

   AAV

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   Aug. 11, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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6. pCDNA5FRT/TO_HA-mAID-nanobody

   Plasmid

   #117713

   Purpose

   Expression of tetracycline-inducible HA-tagged lysine-less mAID fused to lysine-less vhhGFP4 (mAID-nanobody)

   Depositor

   Joerg Mansfeld

   Article

   Daniel et al Nat Commun. 2018 Aug 17;9(1):3297. doi:

   Insert

   mAID-nanobody
   

   Use

   Tags

   triple HA

   Expression

   Mammalian

   Mutation

   codon optimized mAID for murine expression

   Promoter

   CMV

   Available Since

   Dec. 4, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pcDNA5/FRT/TO-GAlphaQ-RLuc8

   Plasmid

   #140982

   Purpose

   Encodes a G alpha subunit (GNAQ) containing RLuc8 as an optimal component of a BRET2 biosensor for studying heterotrimeric G protein dissociation

   Depositor

   Bryan Roth

   Article

   Olsen et al Nat Chem Biol. 2020 May 4. pii: 10.1038/

   Insert

   GAlphaQ-RLuc8 (GNAQ Human)
   

   Use

   Luciferase

   Tags

   Expression

   Mammalian

   Mutation

   RLuc8 and flanking SGGGGS linkers have been inser…

   Promoter

   CMV

   Available Since

   June 26, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pFRT/TO/FLAG/HA-DEST PABPC4

   Plasmid

   #19882

   Depositor

   Thomas Tuschl

   Article

   Landthaler et al RNA. 2008 Dec;14(12):2580-96. doi: 10.12

   Insert

   Poly(A) binding protein, cytoplasmic 4 (PABPC4 Human)
   

   Use

   Tags

   FLAG/HA

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   Dec. 31, 2008

   Availability

   Academic Institutions and Nonprofits only

   

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9. pcDNA5-FRT/TO-eGFP-53BP1

   Plasmid

   #60813

   Purpose

   mammalian expression vector

   Depositor

   Daniel Durocher

   Article

   Fradet-Turcotte et al Nature. 2013 Jul 4;499(7456):50-4. doi:

   Insert

   53BP1 (TP53BP1 Human)
   

   Use

   Tags

   eGFP

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   Dec. 9, 2014

   Availability

   Industry, Academic Institutions, and Nonprofits

   

   Enlarge

10. pFRT/TO/HIS/FLAG/HA-MYBBP1A

    Plasmid

    #38084

    Depositor

    Markus Landthaler

    Article

    Baltz et al Mol Cell. 2012 Jun 8;46(5):674-90.

    Insert

    MYBBP1A MYB binding protein (P160) 1a (MYBBP1A Human)
    

    Use

    Tags

    HIS/FLAG/HA

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Sept. 24, 2012

    Availability

    Academic Institutions and Nonprofits only

    

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11. pEF5B-FRT-cilia-APEX

    Plasmid

    #73186

    Purpose

    Expresses cilia-APEX in mammalian cells

    Depositor

    Maxence Nachury

    Article

    Mick et al Dev Cell. 2015 Nov 23;35(4):497-512. doi

    Insert

    cilia-APEX (Nphp3 Glycine max, Mouse)
    

    Use

    Tags

    internal GFP tag

    Expression

    Mammalian

    Mutation

    Promoter

    EF1alpha

    Available Since

    March 7, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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12. pCDNA5 FRT TO FLAG LRRK1

    Plasmid

    #232062

    Purpose

    Expresses FLAG-LRRK1 in mammalian cells

    Depositor

    Samara Reck-Peterson

    Insert

    LRRK1 (LRRK1 Human)
    

    Use

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Feb. 11, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pcDNA5/FRT/TO HIS DNAJB1

    Plasmid

    #19549

    Depositor

    Harm Kampinga

    Article

    Hageman et al Cell Stress Chaperones. 2009 Jan;14(1):1

    Insert

    DnaJ (Hsp40) homolog, subfamily B, member 1 (DNAJB1 Human)
    

    Use

    Tags

    His

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Oct. 6, 2008

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pcDNA5/FRT/TO-GAlphasL-RLuc8

    Plasmid

    #140981

    Purpose

    Encodes a G alpha subunit (GNAS1) containing RLuc8 as an optimal component of a BRET2 biosensor for studying heterotrimeric G protein dissociation

    Depositor

    Bryan Roth

    Article

    Olsen et al Nat Chem Biol. 2020 May 4. pii: 10.1038/

    Insert

    GAlphasL-RLuc8 (GNAS Human)
    

    Use

    Luciferase

    Tags

    Expression

    Mammalian

    Mutation

    RLuc8 and flanking SGGGGS linkers have been inser…

    Promoter

    CMV

    Available Since

    June 26, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pcDNA5/FRT/TO-GAlphaz-RLuc8

    Plasmid

    #140978

    Purpose

    Encodes a G alpha subunit (GNAZ) containing RLuc8 as an optimal component of a BRET2 biosensor for studying heterotrimeric G protein dissociation

    Depositor

    Bryan Roth

    Article

    Olsen et al Nat Chem Biol. 2020 May 4. pii: 10.1038/

    Insert

    GAlphaz-Rluc8 (GNAZ Human)
    

    Use

    Luciferase

    Tags

    Expression

    Mammalian

    Mutation

    RLuc8 and flanking SGGGGS linkers have been inser…

    Promoter

    CMV

    Available Since

    June 26, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pcDNA5/FRT/TO-GAlphai3-RLuc8

    Plasmid

    #140975

    Purpose

    Encodes a G alpha subunit (GNAI3) containing RLuc8 as an optimal component of a BRET2 biosensor for studying heterotrimeric G protein dissociation

    Depositor

    Bryan Roth

    Article

    Olsen et al Nat Chem Biol. 2020 May 4. pii: 10.1038/

    Insert

    GAlphai3-RLuc8 (GNAI3 Human)
    

    Use

    Luciferase

    Tags

    Expression

    Mammalian

    Mutation

    RLuc8 and flanking SGGGGS linkers have been inser…

    Promoter

    CMV

    Available Since

    June 26, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pcDNA5/FRT/TO-GAlphaoA-RLuc8

    Plasmid

    #140976

    Purpose

    Encodes a G alpha subunit (GNAO1 Isoform Alpha 1) containing RLuc8 as an optimal component of a BRET2 biosensor for studying heterotrimeric G protein dissociation

    Depositor

    Bryan Roth

    Article

    Olsen et al Nat Chem Biol. 2020 May 4. pii: 10.1038/

    Insert

    GAlphaoA-RLuc8 (GNAO1 Human)
    

    Use

    Luciferase

    Tags

    Expression

    Mammalian

    Mutation

    RLuc8 and flanking SGGGGS linkers have been inser…

    Promoter

    CMV

    Available Since

    June 26, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pcDNA5/FRT/TO-GAlphai2-RLuc8

    Plasmid

    #140974

    Purpose

    Encodes a G alpha subunit (GNAI2) containing RLuc8 as an optimal component of a BRET2 biosensor for studying heterotrimeric G protein dissociation

    Depositor

    Bryan Roth

    Article

    Olsen et al Nat Chem Biol. 2020 May 4. pii: 10.1038/

    Insert

    GAlphai2-Rluc8 (GNAI2 Human)
    

    Use

    Luciferase

    Tags

    Expression

    Mammalian

    Mutation

    RLuc8 and flanking SGGGGS linkers have been inser…

    Promoter

    CMV

    Available Since

    June 26, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pcDNA5/FRT/TO-GAlphaGustducin-RLuc8

    Plasmid

    #140979

    Purpose

    Encodes a G alpha subunit (GNAT3) containing RLuc8 as an optimal component of a BRET2 biosensor for studying heterotrimeric G protein dissociation

    Depositor

    Bryan Roth

    Article

    Olsen et al Nat Chem Biol. 2020 May 4. pii: 10.1038/

    Insert

    GAlphaGustducin-RLuc8 (GNAT3 Human)
    

    Use

    Luciferase

    Tags

    Expression

    Mammalian

    Mutation

    RLuc8 and flanking SGGGGS linkers have been inser…

    Promoter

    CMV

    Available Since

    June 26, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pCDNA5/FRT_CMV_NES-Caprola_01-mEGFP

    Plasmid

    #194681

    Purpose

    CMV driven expression of the calcium recorder Caprola_01 in mammalian cell lines fused to mEGFP

    Depositor

    Kai Johnsson

    Article

    Huppertz et al Science. 2024 Feb 23;383(6685):890-897.

    Insert

    Caprola_01-mEGFP
    

    Use

    Tags

    mEGFP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    Feb. 23, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge