We narrowed to 49,324 results for: Tat

1. pAD-CMV-STAT5A-DNL-CMV-GFP

   Plasmid

   #83253

   Purpose

   Adenoviral expression of STAT5A dominant negative with co-expression of GFP

   Depositor

   Andrew Brooks

   Article

   Legrand et al J Invest Dermatol. 2016 Sep;136(9):1781-

   Inserts

   Stat5A (Stat5a Mouse)
   eGFP
   

   Use

   Adenoviral

   Tags

   Expression

   Mutation

   C-terminal deletion of 351 nucleotides (dominant …

   Promoter

   CMV

   Available since

   July 3, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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2. Fibronectin-human-mutated N-terminal (N), V1 and V2 Glycosylation sites

   Plasmid

   #120407

   Purpose

   enables eukaryotic expression of human plasma fibronectin in which all three O-glycosylation sites were mutated from threonine to serine

   Depositor

   Inaam Nakchbandi

   Article

   Sens et al J Bone Miner Res. 2017 Jan;32(1):70-81.

   Insert

   Fibronectin (FN1 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Contains complete variable region with mutations …

   Promoter

   CMV

   Available since

   Jan. 23, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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3. pRS416-PHO5-GFP-2xOsh2 PH domain EE mutation

   Plasmid

   #58830

   Purpose

   Expresses GFP-Tagged and mutated Osh2 PH domain in yeast

   Depositor

   Tim Levine

   Article

   Roy et al J Biol Chem. 2004 Oct 22;279(43):44683-9

   Insert

   2 x Osh2 PH domain (OSH2 Budding Yeast)
   

   Use

   Tags

   GFP and Myc

   Expression

   Yeast

   Mutation

   changed Lysine 307 to Glutamate and Arginine 309 …

   Promoter

   PHO5

   Available since

   Sept. 30, 2014

   Availability

   Academic Institutions and Nonprofits only

4. pGL3-Basic-Fascin-Promoter 402 mutation

   Plasmid

   #89825

   Purpose

   Fascin promoter for luciferase assay

   Depositor

   Shengyu Yang

   Article

   Sun et al J Biol Chem. 2013 Dec 27;288(52):36971-8

   Insert

   Fascin1 (FSCN1 Human)
   

   Use

   Luciferase

   Tags

   Expression

   Mutation

   -370 Smad binding element mutated from CAGAC to T…

   Promoter

   SV40

   Available since

   July 5, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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5. pET11a asyn Q79N (Q109H) (TAC-->TAT)

   Plasmid

   #105736

   Purpose

   Bacterial expression of mutant human alpha synuclein

   Depositor

   Hilal Lashuel

   Insert

   alpha-synuclein Q79N (Q109H) (TAC-->TAT) (SNCA Human)
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Q79N/Q109H

   Promoter

   T7

   Available since

   March 29, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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6. pJRL2 PHO5(TATA-A1T6)pr ECFP (EB#1560)

   Plasmid

   #14846

   Depositor

   Erin O'Shea

   Article

   Raser et al Science. 2004 Jun 18. 304(5678):1811-4.

   Insert

   PHO5 promoter TATA-A1T6 (PHO5 Budding Yeast)
   

   Use

   Tags

   ECFP

   Expression

   Yeast

   Mutation

   TATA-A1T6. TATA box mutated from TATATAAG to AATA…

   Promoter

   Available since

   May 25, 2007

   Availability

   Academic Institutions and Nonprofits only

7. pRS406-PHO5-GFP-CERT PH domain EE mutation

   Plasmid

   #58833

   Purpose

   Expresses GFP-Tagged and mutated CERT PH domain in yeast

   Depositor

   Tim Levine

   Article

   Roy et al J Biol Chem. 2004 Oct 22;279(43):44683-9

   Insert

   collagen, type IV, alpha 3 binding protein PH domain (CERT1 Human)
   

   Use

   Tags

   GFP and Myc

   Expression

   Yeast

   Mutation

   changed Arginine 43 to Glutamate

   Promoter

   PHO5

   Available since

   Aug. 27, 2014

   Availability

   Academic Institutions and Nonprofits only

8. pDONR221 hNLRP3 R262W (disease-associated mutation)

   Plasmid

   #218647

   Purpose

   Gateway shuttling vector for cloning NLRP3-R262W (no stop codon)

   Depositor

   Wade Harper

   Article

   Hollingsworth et al bioRxiv 2024.04.19.590338

   Insert

   NLRP3 (NLRP3 Human)
   

   Use

   Gateway entry

   Tags

   Expression

   Mutation

   R262W

   Promoter

   Available since

   May 3, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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9. pCDNA3-SREBP-1 S1P and S2P mutations (U124AFG200-5)

   Plasmid

   #210767

   Purpose

   Mammalian expression of SREBP-1 (isoform 1) with mutations in residues reported to be critical for S1P and S2P cleavage.

   Depositor

   Allan Weissman

   Article

   Yang et al J Biol Chem. 2023 Oct 12;299(12):105346.

   Insert

   SREBP-1 (SREBF1 Human)
   

   Use

   Tags

   E-tag and T7x2

   Expression

   Mammalian

   Mutation

   DRSR 514-517 to AS; 557 R to A; multiple silent m…

   Promoter

   Available since

   April 8, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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10. pProEx-Htb-cytATL2(R323Q)-6Gly-sfGFP

    Plasmid

    #124066

    Purpose

    E. coli. exp. and purif. of X. laevis cytATL-R232Q fragment fused to GFP in c-terminus.

    Depositor

    Tom Rapoport

    Article

    Wang et al Elife. 2016 Sep 13;5. pii: e18605. doi:

    Insert

    cytATL-R232Q-sfGFP
    

    Use

    Tags

    sfGFP

    Expression

    Bacterial

    Mutation

    R232Q

    Promoter

    trc

    Available since

    May 6, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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11. Fibronectin-human-mutated N-terminal (N) O-Glycosylation site

    Plasmid

    #120404

    Purpose

    enables eukaryotic expression of human plasma fibronectin in which the first O-glycosylation site was mutated from threonine to serine

    Depositor

    Inaam Nakchbandi

    Article

    Sens et al J Bone Miner Res. 2017 Jan;32(1):70-81.

    Insert

    Fibronectin (FN1 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Containing complete variable region with a mutati…

    Promoter

    CMV

    Available since

    Jan. 23, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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12. pET11a asyn Q79N Q109N (TAC-->TAT)

    Plasmid

    #105738

    Purpose

    Bacterial expression of mutant human alpha synuclein

    Depositor

    Hilal Lashuel

    Insert

    alpha-synuclein Q79N Q109N (TAC-->TAT) (SNCA Human)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Q79N/Q109N

    Promoter

    T7

    Available since

    March 29, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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13. pJRL2 GAL1(TATA-A1T6)pr VYFP (EB#1573)

    Plasmid

    #14849

    Depositor

    Erin O'Shea

    Article

    Raser et al Science. 2004 Jun 18. 304(5678):1811-4.

    Insert

    GAL1 promoter TATA-A1T6 (GAL1 Budding Yeast)
    

    Use

    Tags

    VYFP

    Expression

    Yeast

    Mutation

    TATA-A1T6. TATA box mutated from TATATAAG to AATA…

    Promoter

    Available since

    May 25, 2007

    Availability

    Academic Institutions and Nonprofits only

14. pJRL2 GAL1(TATA-A1T6)pr ECFP (EB#1574)

    Plasmid

    #14850

    Depositor

    Erin O'Shea

    Article

    Raser et al Science. 2004 Jun 18. 304(5678):1811-4.

    Insert

    GAL1 promoter TATA-A1T6 (GAL1 Budding Yeast)
    

    Use

    Tags

    ECFP

    Expression

    Yeast

    Mutation

    TATA-A1T6. TATA box mutated from TATATAAG to AATA…

    Promoter

    Available since

    May 25, 2007

    Availability

    Academic Institutions and Nonprofits only

15. p4xSTAT6-Luc2P

    Plasmid

    #35554

    Depositor

    Axel Nohturfft

    Insert

    4xStat6 promoter
    

    Use

    Luciferase

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    4xStat6

    Available since

    March 29, 2012

    Availability

    Academic Institutions and Nonprofits only

    

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16. eGFP STAT1 WT

    Plasmid

    #12301

    Depositor

    Alan Perantoni

    Article

    Timofeeva et al Oncogene. 2006 Dec 7;25(58):7555-64. doi

    Insert

    STAT1 (STAT1 Human)
    

    Use

    Tags

    eGFP

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Sept. 1, 2006

    Availability

    Academic Institutions and Nonprofits only

    

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17. Z-lock αTAT

    Plasmid

    #175290

    Purpose

    light-controlled analog of the alpha-tubulin acetylase αTAT

    Depositor

    Klaus Hahn

    Article

    Stone et al Nat Chem Biol. 2019 Dec;15(12):1183-1190

    Insert

    Z-lock αTAT
    

    Use

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    αTAT (236 amino acids)

    Promoter

    Available since

    Nov. 5, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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18. STAT3-GFP

    Plasmid

    #110495

    Purpose

    reporter of STAT3 signaling activity

    Depositor

    Michael Lewis

    Article

    Wei et al Stem Cells. 2014 Oct;32(10):2571-82. doi

    Insert

    4 repeats of STAT3 binding site M67 at GFP promoter region
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    May 25, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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19. stathmin-GFP

    Plasmid

    #86782

    Purpose

    Mammalian expression of stathmin-GFP

    Depositor

    Lynne Cassimeris

    Article

    Ringhoff et al Mol Biol Cell. 2009 Aug;20(15):3451-8. d

    Insert

    stathmin (STMN1 Human)
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Feb. 1, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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20. pLEGFP-WT-STAT3

    Plasmid

    #71450

    Purpose

    Retroviral expression of WT-STAT3. Please note that this plasmid contains WT-STAT3 tagged with FLAG, not GFP.

    Depositor

    George Stark

    Article

    Dasgupta et al J Biol Chem. 2014 Oct 31;289(44):30763-7

    Insert

    STAT3 (STAT3 Human)
    

    Use

    Retroviral

    Tags

    FLAG

    Expression

    Mutation

    Promoter

    CMV

    Available since

    Nov. 30, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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