Addgene

We narrowed to 26,843 results for: Tat

Showing: 501 - 520 of 26843 results

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opCas12a-T8N (pZZ34, TAT-6MycNLS-opCas12a-2SV40NLS-sfGFP)

Plasmid

#199605

Purpose

Expression of cell permeable opAsCas12a in E. Coli.

Depositor

Junwei Shi

Article

Zhang et al Nat Biotechnol. 2023 Apr 24. doi: 10.103

Insert

opCas12a-T8N

Use

CRISPR

Tags

6xHis, Twin-strep, SUMO

Expression

Bacterial

Mutation

Promoter

Available since

April 28, 2023

Availability

Academic Institutions and Nonprofits only

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Clone 16 - 2 HH06 pFUSE-IgG1-Knob-mutation knob-clone6

Plasmid

#192184

Purpose

Clone 16 (HH06) pFUSE-IgG1-Knob-mutation knob-clone6

Depositor

Sidi Chen

Article

Peng et al Nat Commun. 2022 Mar 28;13(1):1638. doi:

Insert

Clone 6 heavy chain (HH06)

Use

Tags

Expression

Mammalian

Mutation

NA

Promoter

Available since

Dec. 8, 2022

Availability

Academic Institutions and Nonprofits only

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Clone 16 - 1 HH02 pFUSE-IgG1-Hole mutation-hole-clone2

Plasmid

#192183

Purpose

Clone 16 (HH02) pFUSE-IgG1-Hole mutation-hole-clone2

Depositor

Sidi Chen

Article

Peng et al Nat Commun. 2022 Mar 28;13(1):1638. doi:

Insert

Clone 2 heavy chain (HH02)

Use

Tags

Expression

Mammalian

Mutation

NA

Promoter

Available since

Dec. 8, 2022

Availability

Academic Institutions and Nonprofits only

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pMOS013: mito-roGFP2-Grx1 glutathione redox sensor (mitochondrial)

Plasmid

#163056

Purpose

mito-roGFP2-Grx1 glutathione redox sensor (mitochondrial)

Depositor

Adam Cohen

Article

Werley et al Nat Commun. 2020 Aug 4;11(1):3881. doi:

Insert

mito-roGFP2-Grx1

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

March 9, 2021

Availability

Academic Institutions and Nonprofits only

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HisGb1-STAT1(710-750) + CBP TAZ2 (1764-1855)

Plasmid

#99342

Purpose

Bacterial expression of mouse CBP TAZ2 domain by coexpression with HisGB1 tagged STAT1 TAD

Depositor

Peter Wright

Article

Wojciak et al EMBO J. 2009 Apr 8;28(7):948-58. doi: 10

Inserts

STAT1 (710-750) (STAT1 Human)
CBP (1764-1855)

Use

Tags

His6GB1

Expression

Bacterial

Mutation

Promoter

T7

Available since

Sept. 13, 2017

Availability

Academic Institutions and Nonprofits only

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pX330-Flag-HiFi SpCas9 (without sgRNA; with silent mutations)

Plasmid

#126778

Purpose

Expression plasmid for human codon-optimized increased fidelity HiFi SpCas9 (without U6-sgRNA coding sequence, with silent mutations)

Depositor

Ervin Welker

Article

Kulcsar et al Nat Commun. 2020 Mar 6;11(1):1223. doi:

Insert

HiFi SpCas9

Use

CRISPR

Tags

3xFLAG and NLS

Expression

Mammalian

Mutation

R691A

Promoter

CBh

Available since

March 6, 2020

Availability

Academic Institutions and Nonprofits only

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pRDAX-619 C-Tag Venus210[2] L10 Stat3(R609Q) mCherry

Plasmid

#130094

Purpose

PPI (BiFC/TriFC) assay vector

Depositor

Gary Lewin

Insert

Stat3(R609Q)

Use

Tags

Expression

Mammalian

Mutation

R609Q

Promoter

Available since

Sept. 9, 2020

Availability

Academic Institutions and Nonprofits only

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pX330-Flag-Sniper SpCas9 (without sgRNA; with silent mutations)

Plasmid

#126777

Purpose

Expression plasmid for human codon-optimized increased fidelity Sniper SpCas9 (without U6-sgRNA coding sequence, with silent mutations)

Depositor

Ervin Welker

Article

Kulcsar et al Nat Commun. 2020 Mar 6;11(1):1223. doi:

Insert

Sniper SpCas9

Use

CRISPR

Tags

3xFLAG and NLS

Expression

Mammalian

Mutation

F539S, M763I, K890N

Promoter

CBh

Available since

March 6, 2020

Availability

Academic Institutions and Nonprofits only

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pTwist-SARS-CoV-2 Spike-deltaC18 (P.2/B.1.1.248) - No RBD Mutations

Plasmid

#212385

Purpose

Encodes SARS-CoV-2 spike D614G,V1176F for pseudovirus production

Depositor

Alejandro Balazs

Article

Roederer et al medRxiv [Preprint]. 2024 Mar 7:2024.03.0

Insert

SARS-CoV-2 Spike D614G,V1176F (S Severe acute respiratory syndrome coronavirus 2)

Use

Tags

Expression

Mammalian

Mutation

D614G,V1176F

Promoter

CAG

Available since

Sept. 13, 2024

Availability

Academic Institutions and Nonprofits only

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pRDAX-566 N-Tag GFP[1] L10 Stat3(R609Q) EBPF2

Plasmid

#130113

Purpose

PPI (BiFC/TriFC) assay vector

Depositor

Gary Lewin

Insert

Stat3(R609Q)

Use

Tags

Expression

Mammalian

Mutation

R609Q

Promoter

Available since

Sept. 9, 2020

Availability

Academic Institutions and Nonprofits only

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pRDAX-567 C-Tag GFP[1] L20 Stat3(R609Q) EBPF2

Plasmid

#130114

Purpose

PPI (BiFC/TriFC) assay vector

Depositor

Gary Lewin

Insert

Stat3(R609Q)

Use

Tags

Expression

Mammalian

Mutation

R609Q

Promoter

Available since

Sept. 9, 2020

Availability

Academic Institutions and Nonprofits only

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pRDAX-568 N-Tag GFP[1] L20 Stat3(R609Q) EBPF2

Plasmid

#130115

Purpose

PPI (BiFC/TriFC) assay vector

Depositor

Gary Lewin

Insert

Stat3(R609Q)

Use

Tags

Expression

Mammalian

Mutation

R609Q

Promoter

Available since

Sept. 9, 2020

Availability

Academic Institutions and Nonprofits only

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pRDAX-569 C-Tag GFP[1] L32 Stat3(R609Q) EBPF2

Plasmid

#130116

Purpose

PPI (BiFC/TriFC) assay vector

Depositor

Gary Lewin

Insert

Stat3(R609Q)

Use

Tags

Expression

Mammalian

Mutation

R609Q

Promoter

Available since

Sept. 9, 2020

Availability

Academic Institutions and Nonprofits only

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pRDAX-570 N-Tag GFP[1] L32 Stat3(R609Q) EBPF2

Plasmid

#130117

Purpose

PPI (BiFC/TriFC) assay vector

Depositor

Gary Lewin

Insert

Stat3(R609Q)

Use

Tags

Expression

Mammalian

Mutation

R609Q

Promoter

Available since

Sept. 9, 2020

Availability

Academic Institutions and Nonprofits only

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pRDAX-571 C-Tag GFP[2] L10 Stat3(R609Q) mCherry

Plasmid

#130118

Purpose

PPI (BiFC/TriFC) assay vector

Depositor

Gary Lewin

Insert

Stat3(R609Q)

Use

Tags

Expression

Mammalian

Mutation

R609Q

Promoter

Available since

Sept. 9, 2020

Availability

Academic Institutions and Nonprofits only

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pRDAX-572 N-Tag GFP[2] L10 Stat3(R609Q) mCherry

Plasmid

#130119

Purpose

PPI (BiFC/TriFC) assay vector

Depositor

Gary Lewin

Insert

Stat3(R609Q)

Use

Tags

Expression

Mammalian

Mutation

R609Q

Promoter

Available since

Sept. 9, 2020

Availability

Academic Institutions and Nonprofits only

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pRDAX-573 C-Tag GFP[2] L20 Stat3(R609Q) mCherry

Plasmid

#130120

Purpose

PPI (BiFC/TriFC) assay vector

Depositor

Gary Lewin

Insert

Stat3(R609Q)

Use

Tags

Expression

Mammalian

Mutation

R609Q

Promoter

Available since

Sept. 9, 2020

Availability

Academic Institutions and Nonprofits only

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pRDAX-574 N-Tag GFP[2] L20 Stat3(R609Q) mCherry

Plasmid

#130121

Purpose

PPI (BiFC/TriFC) assay vector

Depositor

Gary Lewin

Insert

Stat3(R609Q)

Use

Tags

Expression

Mammalian

Mutation

R609Q

Promoter

Available since

Sept. 9, 2020

Availability

Academic Institutions and Nonprofits only

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pRDAX-576 N-Tag GFP[2] L32 Stat3(R609Q) mCherry

Plasmid

#130123

Purpose

PPI (BiFC/TriFC) assay vector

Depositor

Gary Lewin

Insert

Stat3(R609Q)

Use

Tags

Expression

Mammalian

Mutation

R609Q

Promoter

Available since

Sept. 9, 2020

Availability

Academic Institutions and Nonprofits only

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pRDAX-617 C-Tag Venus210[1] L32 Stat3(R609Q) EBPF2

Plasmid

#130092

Purpose

PPI (BiFC/TriFC) assay vector

Depositor

Gary Lewin

Insert

Stat3(R609Q)

Use

Tags

Expression

Mammalian

Mutation

R609Q

Promoter

Available since

Sept. 9, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge