We narrowed to 23,134 results for: crispr

1. pCRISPR-RFP entry

   Plasmid

   #192278

   Purpose

   Entry vector for cloning MmCas12m spacers. RFP flanked by MmCas12m CRISPR repeats can be digested out with BbsI.

   Depositor

   John van der Oost

   Article

   Wu et al Mol Cell. 2022 Dec 1;82(23):4487-4502.e7

   Insert

   RFP flanked by type V-M CRISPR repeat sequences
   

   Use

   CRISPR

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   PJ23119

   Available since

   May 24, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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2. pWS5321-CRISPRai-Markerless

   Plasmid

   #182828

   Purpose

   Saccharomyces cerevisiae inducible CRISPRai vector - markerless

   Depositor

   Rodrigo Ledesma-Amaro

   Article

   Shaw et al Nat Commun. 2022 Aug 25;13(1):4984. doi:

   Type

   Empty backbone

   Use

   CRISPR

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Nov. 10, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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3. pLentiCRISPRv2-sgAAVS1-HepmCherry

   Plasmid

   #192827

   Purpose

   Expresses sgRNA targeting AAVS1 (non-targeting control sgRNA for mouse) and mCherry from hepatocyte-specific promoter

   Depositor

   Kristin Knouse

   Article

   Keys et al Cell Genom. 2022 Dec 14;2(12):100217. do

   Insert

   mCherry
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6 for sgRNA and hepatocyte-specific promoter (HS…

   Available since

   Nov. 23, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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4. lentiCRISPR - CTRL sgRNA

   Plasmid

   #70662

   Purpose

   Expresses human codon-optimized Cas9 protein and puromycin resistance from EFS promoter and a non-targeting sgRNA element from U6 promoter. Lentiviral backbone

   Depositor

   David Sabatini

   Article

   Wang et al Science. 2015 Oct 15. pii: aac7041.

   Insert

   non-targeting sgRNA
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Oct. 13, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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5. POLR2A-N CRISPR pX330

   Plasmid

   #124495

   Purpose

   A CRISPR plasmid for targeting the N-terminus coding region of human POLR2A

   Depositor

   Masato Kanemaki

   Article

   Nagashima et al J Cell Biol. 2019 Mar 1. pii: jcb.201811

   Insert

   POLR2A targeting CRISPR
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   Promoter

   U6 promoter

   Available since

   April 12, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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6. CRISPR-StAR1 (Retro)

   Plasmid

   #222687

   Purpose

   CRISPR-screening

   Depositor

   Ulrich Elling

   Article

   Uijttewaal et al Nat Biotechnol. 2024 Dec 16. doi: 10.103

   Type

   Empty backbone

   Use

   CRISPR and Retroviral

   Tags

   Expression

   Mammalian

   Mutation

   PGK without BbsI cutsite

   Promoter

   Available since

   Dec. 17, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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7. CRISPR-StAR2 (Retro)

   Plasmid

   #222688

   Purpose

   CRISPR-screening

   Depositor

   Ulrich Elling

   Article

   Uijttewaal et al Nat Biotechnol. 2024 Dec 16. doi: 10.103

   Type

   Empty backbone

   Use

   CRISPR and Retroviral

   Tags

   Expression

   Mammalian

   Mutation

   PGK without BbsI cutsite

   Promoter

   Available since

   Dec. 17, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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8. CRISPR-StAR3 (Retro)

   Plasmid

   #222689

   Purpose

   CRISPR-screening

   Depositor

   Ulrich Elling

   Article

   Uijttewaal et al Nat Biotechnol. 2024 Dec 16. doi: 10.103

   Type

   Empty backbone

   Use

   CRISPR and Retroviral

   Tags

   Expression

   Mammalian

   Mutation

   PGK without BbsI cutsite

   Promoter

   Available since

   Dec. 17, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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9. LentiCRISPR v2 RB1 sgRNA #1

   Plasmid

   #202516

   Purpose

   Expressing Cas9 and sgRNA targeting human RB1 gene

   Depositor

   Ming Chen

   Article

   Wang et al J Clin Invest. 2023 Mar 16:e166647. doi:

   Insert

   RB1 sgRNA (RB1 Human)
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6

   Available since

   June 14, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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10. pLentiCRISPRv2-Stuffer-HepmCherry

    Plasmid

    #192825

    Purpose

    Contains stuffer sequence into which sgRNAs can be cloned and expresses mCherry from hepatocyte-specific promoter

    Depositor

    Kristin Knouse

    Article

    Keys et al Cell Genom. 2022 Dec 14;2(12):100217. do

    Insert

    mCherry
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Hepatocyte-specific promoter (HS-CRM8-TTRmin modu…

    Available since

    Nov. 23, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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11. lentiCRISPR v2 puro - CRBN (#2)

    Plasmid

    #166241

    Purpose

    sgRNA (#2) to generate a knockout of human CRBN by targeting the start region of Exon1

    Depositor

    Günter Schneider

    Article

    Lier et al Bioorg Chem. 2021 Nov 20:105505. doi: 10

    Insert

    CRBN (CRBN Human)
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Dec. 20, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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12. LentiCRISPR v2-sgLKB1 clone3

    Plasmid

    #162127

    Purpose

    Lentiviral sgRNA plasmid targeting human LKB1

    Depositor

    Boyi Gan

    Article

    Lee et al Nat Cell Biol. 2020 Feb;22(2):225-234. d

    Insert

    sgLKB1 (STK11 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Jan. 26, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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13. pWS5398-CRISPRai-HygR

    Plasmid

    #182837

    Purpose

    Saccharomyces cerevisiae inducible CRISPRai vector - HygR marker

    Depositor

    Rodrigo Ledesma-Amaro

    Article

    Shaw et al Nat Commun. 2022 Aug 25;13(1):4984. doi:

    Type

    Empty backbone

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Nov. 10, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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14. pWS5390-CRISPRai-URA3

    Plasmid

    #182829

    Purpose

    Saccharomyces cerevisiae inducible CRISPRai vector - URA3 marker

    Depositor

    Rodrigo Ledesma-Amaro

    Article

    Shaw et al Nat Commun. 2022 Aug 25;13(1):4984. doi:

    Type

    Empty backbone

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Nov. 10, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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15. LentiCRISPRv2 EGFP sgRNA2

    Plasmid

    #164933

    Purpose

    Expresses EGFP sgRNA2 in mammalian cells

    Depositor

    Ji Luo

    Article

    Yuen et al Nucleic Acids Res. 2017 Nov 16;45(20):12

    Insert

    sgRNA2 targeting Enhanced green fluorescent protein (verified for knockout)
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    EFS promoter

    Available since

    July 19, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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16. pLentiCRISPRv2-sgMaob-HepmCherry

    Plasmid

    #192829

    Purpose

    Expresses sgRNA targeting Maob and mCherry from hepatocyte-specific promoter

    Depositor

    Kristin Knouse

    Article

    Keys et al Cell Genom. 2022 Dec 14;2(12):100217. do

    Insert

    mCherry
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6 for sgRNA and hepatocyte-specific promoter (HS…

    Available since

    Nov. 23, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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17. lentiCRISPR - EGFP sgRNA 4

    Plasmid

    #51763

    Purpose

    Expresses human codon-optimized Cas9 protein and puromycin resistance from EFS promoter and an EGFP targeting synthetic single-guide RNA (sgRNA) element from U6 promoter. Lentiviral backbone.

    Depositor

    Feng Zhang

    Article

    Shalem et al Science. 2014 Jan 3;343(6166):84-7. doi:

    Inserts

    Cas9
    Puromycin resistance
    EGFP sgRNA 4
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EFS and U6

    Available since

    March 11, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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18. lentiCRISPR - EGFP sgRNA 5

    Plasmid

    #51764

    Purpose

    Expresses human codon-optimized Cas9 protein and puromycin resistance from EFS promoter and an EGFP targeting synthetic single-guide RNA (sgRNA) element from U6 promoter. Lentiviral backbone.

    Depositor

    Feng Zhang

    Article

    Shalem et al Science. 2014 Jan 3;343(6166):84-7. doi:

    Inserts

    Cas9
    Puromycin resistance
    EGFP sgRNA 5
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    EFS and U6

    Available since

    March 11, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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19. lentiCRISPR - AAVS1 sgRNA

    Plasmid

    #70661

    Purpose

    Expresses human codon-optimized Cas9 protein and puromycin resistance from EFS promoter and an AAVS1-targeting sgRNA element from U6 promoter. Lentiviral backbone

    Depositor

    David Sabatini

    Article

    Wang et al Science. 2015 Oct 15. pii: aac7041.

    Insert

    sgRNA against AAVS1
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Oct. 29, 2015

    Availability

    Academic Institutions and Nonprofits only

20. pCRISPR Human CASP9 -2

    Plasmid

    #198420

    Purpose

    Expresses Human CASP9 Specific gRNA/Cas9 Complex and Reporter Protein

    Depositor

    Hannah Rabinowich

    Article

    Han et al Autophagy. 2021 Jun;17(6):1393-1409. doi

    Insert

    Human CASP9 Specific gRNA (CASP9 Human)
    

    Use

    CRISPR

    Tags

    Cas9/Orange Fluorescent Protein Reporter

    Expression

    Mammalian

    Mutation

    Promoter

    U6; CMV

    Available since

    April 14, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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