We narrowed to 849 results for: nanobody

1. H2B-mCherry

   Plasmid

   #108880

   Purpose

   Expression of human histone2B fused to mCherry (nuclear marker)

   Depositor

   Rob Parton

   Article

   Ariotti et al PLoS Biol. 2018 Apr 5;16(4):e2005473. do

   Insert

   H2B-mCherry
   

   Use

   Can be used for mammalian expression or in vitro …

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   June 28, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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2. p3E-APEX2-P2A-EGFP

   Plasmid

   #108896

   Purpose

   Multisite gateway vector for 3' tagging with APEX2 (includes cytoplasmic GFP reporter)

   Depositor

   Rob Parton

   Article

   Ariotti et al PLoS Biol. 2018 Apr 5;16(4):e2005473. do

   Insert

   APEX2-P2A-EGFP
   

   Use

   Multisite gateway

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Aug. 30, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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3. pBTSG

   Plasmid

   #159420

   Purpose

   Transient expression of proteins with a C-terminal thermostable GFP (TGP) tag in mammalian cells

   Depositor

   Dianfan Li

   Article

   Cai et al Commun Biol. 2020 Dec 10;3(1):753. doi:

   Insert

   thermostable GFP (TGP)
   

   Use

   Tags

   polyhistidine (poly-His)

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Jan. 7, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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4. pNIR-Fbactin

   Plasmid

   #184677

   Purpose

   NIR-Fb to actin

   Depositor

   Vladislav Verkhusha

   Article

   Oliinyk et al Nat Methods. 2022 May 23. pii: 10.1038/s

   Insert

   NIR-Fbactin
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   June 23, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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5. pETSG

   Plasmid

   #159418

   Purpose

   Expression of proteins with a C-terminal thermostable GFP (TGP) tag in Escherichia coli

   Depositor

   Dianfan Li

   Article

   Cai et al Commun Biol. 2020 Dec 10;3(1):753. doi:

   Insert

   thermostable GFP (TGP)
   

   Use

   Tags

   polyhistidine (poly-His)

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Jan. 7, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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6. pUAS-Cre

   Plasmid

   #50797

   Purpose

   Expression of Cre recombinase under the control of the UAS-promoter

   Depositor

   Connie Cepko

   Article

   Tang et al Cell. 2013 Aug 15;154(4):928-39. doi: 10

   Insert

   Cre recombinase
   

   Use

   Multi-species

   Tags

   Expression

   Mutation

   Promoter

   UAS-hsp70

   Available since

   March 20, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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7. pCAG-Flp-DOG

   Plasmid

   #80084

   Purpose

   Flp recombinase dependent on GFP (Flp-DOG) for expression in mammalian cells

   Depositor

   Connie Cepko

   Article

   Tang et al Elife. 2016 May 20;5. pii: e15312. doi:

   Insert

   Flp-DOG
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CAG

   Available since

   July 22, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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8. pCS2+_NLS-Tir1-HA

   Plasmid

   #117716

   Purpose

   mRNA synthesis of NLS-TIR1-HA for injection

   Depositor

   Joerg Mansfeld

   Article

   Daniel et al Nat Commun. 2018 Aug 17;9(1):3297. doi:

   Insert

   TRANSPORT INHIBITOR RESPONSE 1
   

   Use

   Tags

   HA and NLS

   Expression

   Mammalian

   Mutation

   codon-optimized TIR1 for murine expression

   Promoter

   SP6

   Available since

   Dec. 4, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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9. pBiex-1 Myosin VI HMM_eGFP

   Plasmid

   #82714

   Purpose

   Expresses Myosin VI HMM with a C-terminal eGFP.

   Depositor

   Sivaraj Sivaramakrishnan

   Article

   Sommese et al Protein Sci. 2016 Aug 18. doi: 10.1002/p

   Insert

   Myosin VI
   

   Use

   Tags

   FLAG purification tag and eGFP

   Expression

   Bacterial and Insect

   Mutation

   Promoter

   T7 Promoter

   Available since

   Nov. 3, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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10. pOPINVHH_his

    Plasmid

    #210405

    Purpose

    For expression of VHH with C-terminal his tag

    Depositor

    Ray Owens

    Article

    Eyssen et al Bio Protoc. 2024 Mar 20;14(6):e4962. doi

    Type

    Empty backbone

    Use

    Tags

    his6

    Expression

    Bacterial

    Mutation

    Promoter

    lac

    Available since

    March 26, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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11. BbZIP-cALFA

    Plasmid

    #160498

    Purpose

    For the protein expression of BbZIP with ALFA tag at C-terminus in E. coli

    Depositor

    Motoyuki Hattori

    Article

    Jin et al Commun Biol. 2021 Mar 19;4(1):366. doi:

    Insert

    BbZIP
    

    Use

    Tags

    HRV3C protease cleavage site-ALFA tag-8histidine

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Jan. 4, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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12. pSBtet-2xMARS-nGFP-puro

    Plasmid

    #205239

    Purpose

    DOX inducible expression of two repeats of PLEKHA5 aa 143-271 (K163A and R164A) fused to a HA-tagged GFP nanobody in mammalian cells from the Sleeping Beauty transposon system

    Depositor

    Jeremy Baskin

    Insert

    2xPLEKHA5(143-271)-K163A/R164A-HA-nGFP (PLEKHA5 Human)
    

    Use

    Transposon

    Tags

    HA, Nuclear Export Sequence, mScarlet-i, and nGFP…

    Expression

    Mammalian

    Mutation

    amino acids 143-271 of PLEKHA5 (GenBank reference…

    Promoter

    tight TRE promoter

    Available since

    July 30, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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13. pCAG-GFPmG1

    Plasmid

    #50444

    Purpose

    Expression of a EGFP variant in mammalian cells

    Depositor

    Connie Cepko

    Article

    Tang et al Cell. 2013 Aug 15;154(4):928-39. doi: 10

    Insert

    GFPmG1
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    E143K, N147Q

    Promoter

    CAG

    Available since

    March 20, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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14. pDX_init containing Sb-loop

    Plasmid

    #132697

    Purpose

    pDX_init plasmid containing a loop Sybody for the generation of a qPCR standard curve

    Depositor

    Markus Seeger

    Article

    Zimmermann et al Nat Protoc. 2020 Apr 8. pii: 10.1038/s41

    Insert

    Sb_loop
    

    Use

    M13 phagmid vector

    Tags

    Expression

    Mutation

    Promoter

    lac

    Available since

    Oct. 21, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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15. pME-EGFP-P2A-APEX2-NS

    Plasmid

    #108895

    Purpose

    Multisite gateway vector for 5' tagging with APEX2 (includes cytoplasmic GFP reporter)

    Depositor

    Rob Parton

    Article

    Ariotti et al PLoS Biol. 2018 Apr 5;16(4):e2005473. do

    Insert

    EGFP-P2A-APEX2
    

    Use

    Multisite gateway

    Tags

    Expression

    Mutation

    Promoter

    Available since

    May 19, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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16. pB2AR-miRFP670nano3

    Plasmid

    #184671

    Purpose

    Beta-2-adrenergic receptor internally tagged with miRFP670nano3

    Depositor

    Vladislav Verkhusha

    Article

    Oliinyk et al Nat Methods. 2022 May 23. pii: 10.1038/s

    Insert

    B2AR-miRFP670nano3
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    June 24, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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17. pOPINVHH_cys_his

    Plasmid

    #210403

    Purpose

    For expression of VHH with C-terminal cysteine and his tag

    Depositor

    Ray Owens

    Article

    Eyssen et al Bio Protoc. 2024 Mar 20;14(6):e4962. doi

    Type

    Empty backbone

    Use

    Tags

    cys-his6

    Expression

    Bacterial

    Mutation

    Promoter

    lac

    Available since

    March 26, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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18. pOPINVHH_his_flag

    Plasmid

    #210404

    Purpose

    For expression of VHH with C-terminal his and Flag tags

    Depositor

    Ray Owens

    Article

    Eyssen et al Bio Protoc. 2024 Mar 20;14(6):e4962. doi

    Type

    Empty backbone

    Use

    Tags

    his6-flag

    Expression

    Bacterial

    Mutation

    Promoter

    lac

    Available since

    March 26, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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19. pOPINVHH_sortase_his

    Plasmid

    #210406

    Purpose

    For expression of VHH with C-terminal sortase and his tags

    Depositor

    Ray Owens

    Article

    Eyssen et al Bio Protoc. 2024 Mar 20;14(6):e4962. doi

    Type

    Empty backbone

    Use

    Tags

    sortase-his6

    Expression

    Bacterial

    Mutation

    Promoter

    lac

    Available since

    March 26, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pOPINVHH_snap_his

    Plasmid

    #210407

    Purpose

    For expression of VHH with C-terminal snap protein and his tag

    Depositor

    Ray Owens

    Article

    Eyssen et al Bio Protoc. 2024 Mar 20;14(6):e4962. doi

    Type

    Empty backbone

    Use

    Tags

    His6

    Expression

    Bacterial

    Mutation

    Promoter

    lac

    Available since

    March 26, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge