We narrowed to 24,358 results for: nes

1. pCMV:: NES-FKBP-iLID-LRP6c(△1-64)

   Plasmid

   #221775

   Purpose

   Expresses NES-FKBP-iLID-LRP6c(△1-64) component in mammalian cells

   Depositor

   Dongmin Lee

   Article

   Lee et al iScience. 2023 Feb 19;26(3):106233. doi:

   Insert

   pCMV:: NES-FKBP-iLID-LRP6c
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV promoter

   Available since

   Aug. 29, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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2. pcDNA5-FRT/T0-3XFLAG-NESm-NFATC2IP

   Plasmid

   #221445

   Purpose

   Mammalian expression vector of triple Flag tagged NFATC2IP ORF with mutant nuclear export signal (NESm) at N-terminus

   Depositor

   Daniel Durocher

   Article

   Cho et al Genes Dev. 2024 Apr 17;38(5-6):233-252.

   Insert

   NFATC2IP (NFATC2IP Human)
   

   Use

   Tags

   3XFlag and Mutant Nuclear Export Signal (mNES)

   Expression

   Mammalian

   Mutation

   mutant NES (NESm: NLVDLQKKLEEAKADEQQ (PMID: 10739…

   Promoter

   CMV

   Available since

   July 3, 2024

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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3. pcDNA5-FRT/T0-eGFP-NES-NFATC2IP

   Plasmid

   #221446

   Purpose

   Mammalian expression vector of eGFP tagged NFATC2IP ORF with nuclear export signal (NES) at its N-terminus

   Depositor

   Daniel Durocher

   Article

   Cho et al Genes Dev. 2024 Apr 17;38(5-6):233-252.

   Insert

   NFATC2IP (NFATC2IP Human)
   

   Use

   Tags

   Nuclear Export Signal (NES) and eGFP

   Expression

   Mammalian

   Mutation

   NES (NLVDLQKKLEELELDEQQ) inserted, 1st Methionine…

   Promoter

   CMV

   Available since

   July 3, 2024

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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4. pcDNA5-FRT/T0-3XFLAG-NES-NFATC2IP

   Plasmid

   #221444

   Purpose

   Mammalian expression vector of triple Flag tagged NFATC2IP ORF with nuclear export signal (NES) at its N-terminus

   Depositor

   Daniel Durocher

   Article

   Cho et al Genes Dev. 2024 Apr 17;38(5-6):233-252.

   Insert

   NFATC2IP (NFATC2IP Human)
   

   Use

   Tags

   3XFlag and Nuclear Export Signal (NES)

   Expression

   Mammalian

   Mutation

   NES (NLVDLQKKLEELELDEQQ)inserted, 1st Methionine …

   Promoter

   CMV

   Available since

   July 3, 2024

   Availability

   Industry, Academic Institutions, and Nonprofits

   

   Enlarge

5. pTol2_elavl3(HuC)_NES-Caprola_on-mEGFP

   Plasmid

   #194697

   Purpose

   HuC driven expression of the consitutively active calcium recorder positive control Caprola_on fused to mEGFP for neuronal expression in zebrafish

   Depositor

   Kai Johnsson

   Article

   Huppertz et al Science. 2024 Feb 23;383(6685):890-897.

   Insert

   Caprola_on-mEGFP
   

   Use

   Zebrafish expression

   Tags

   mEGFP

   Expression

   Mutation

   Promoter

   elavl3(HuC)

   Available since

   Feb. 23, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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6. pTol2_elavl3(HuC)_NES-Caprola_off-mEGFP

   Plasmid

   #194698

   Purpose

   HuC driven expression of the inactive calcium recorder negative control Caprola_off fused to mEGFP for neuronal expression in zebrafish

   Depositor

   Kai Johnsson

   Article

   Huppertz et al Science. 2024 Feb 23;383(6685):890-897.

   Insert

   Caprola_off-mEGFP
   

   Use

   Zebrafish expression

   Tags

   mEGFP

   Expression

   Mutation

   Promoter

   elavl3(HuC)

   Available since

   Feb. 23, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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7. pJFRC7-20XUAS-IVS-NES_Caprola5-mEGFP

   Plasmid

   #205704

   Purpose

   Gal4 driven expression of EGFP-Caprola5 for targeted expression in Drosophila flies

   Depositor

   Kai Johnsson

   Article

   Huppertz et al Science. 2024 Feb 23;383(6685):890-897.

   Insert

   Caprola5-EGFP
   

   Use

   Tags

   Expression

   Insect

   Mutation

   Promoter

   20 Gal4

   Available since

   Feb. 23, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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8. pPBpuro-UbC-4493NES(Booster-PKA)

   Plasmid

   #184711

   Purpose

   PKA biosensor

   Depositor

   Michiyuki Matsuda

   Article

   Watabe et al eLife. 2024 Jan 26;12:RP86727. doi: 10.7

   Insert

   Booster-PKA
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Jan. 29, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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9. pEN_TT mCh-V5-NES-EGFP-mCBP80

   Plasmid

   #192313

   Purpose

   Gateway entry vector for an inducible mCh-V5-NES-EGFP-mCBP80

   Depositor

   Kevin Janes

   Article

   Wang et al Nat Commun. 2023 Apr 13;14(1):2110. doi:

   Insert

   mCh-V5-NES-EGFP-mCBP80
   

   Use

   Gateway entry vector

   Tags

   3xFLAG

   Expression

   Mutation

   Promoter

   Available since

   April 28, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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10. pEN_TT mCh-V5-NES-EGFP-IBB

    Plasmid

    #192314

    Purpose

    Gateway entry vector for an inducible mCh-V5-NES-EGFP-IBB

    Depositor

    Kevin Janes

    Article

    Wang et al Nat Commun. 2023 Apr 13;14(1):2110. doi:

    Insert

    mCh-V5-NES-EGFP-IBB
    

    Use

    Gateway entry vector

    Tags

    3xFLAG

    Expression

    Mutation

    Promoter

    Available since

    April 28, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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11. pcDNA3.1(+)-myc-OGA(GS-544)C181_NES

    Plasmid

    #193998

    Purpose

    plasmid encoding myc-tagged OGA(GS-544)C181_NES

    Depositor

    Christina Woo

    Article

    Ge et al ACS Chem Biol. 2023 Jan 4. doi: 10.1021/

    Insert

    myc-OGA(GS-544)C181_NES
    

    Use

    Tags

    myc

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Jan. 11, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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12. pCSD_2xNES-FRB-ZFPTS2-3xFLAG-2xNLS

    Plasmid

    #107303

    Purpose

    Expresses ZFP-VEGFA-TS2 fused to FRB in mammalian cells

    Depositor

    Scot Wolfe

    Insert

    ZFP_VEGFA-TS2
    

    Use

    CRISPR

    Tags

    2xNES, 3x Flag, 2xNLS, and FRB

    Expression

    Mammalian

    Mutation

    Promoter

    CMV IE94

    Available since

    Nov. 15, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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13. pCSD_2xNES-FRB-ZFPTS3-3xFLAG-2xNLS

    Plasmid

    #107304

    Purpose

    Expresses ZFP-VEGFA-TS3 fused to FRB in mammalian cells

    Depositor

    Scot Wolfe

    Insert

    ZFP_VEGFA-TS3
    

    Use

    CRISPR

    Tags

    2xNES, 3x Flag, 2xNLS, and FRB

    Expression

    Mammalian

    Mutation

    Promoter

    CMV IE94

    Available since

    Nov. 15, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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14. pcDNA3-mCherry-PKCsub-RLuc8N-NES

    Plasmid

    #138213

    Purpose

    Encodes substrate fragment of luminescence-based bimolecular PKC activity reporter (LumCKAR); cytosol targeted. Use in conjunction with pcDNA3-RLuc8C-FHA1-NES.

    Depositor

    Jin Zhang

    Article

    Herbst et al J Am Chem Soc. 2011 Apr 20;133(15):5676-

    Insert

    mCherry-PKCsub-RLuc8N-NES
    

    Use

    Tags

    6xHIS - T7 tag (gene 10 leader) - Xpress (TM) tag…

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Availability

    Academic Institutions and Nonprofits only

    

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15. pcDNA3-mCherry-PKCsub(T/A)-RLuc8N-NES

    Plasmid

    #138299

    Purpose

    Encodes negative-control substrate fragment of luminescence-based bimolecular PKC activity reporter (LumCKAR); cytosol targeted. Use in conjunction with pcDNA3-RLuc8C-FHA1-NES.

    Depositor

    Jin Zhang

    Article

    Herbst et al J Am Chem Soc. 2011 Apr 20;133(15):5676-

    Insert

    mCherry-PKCsub(T/A)-RLuc8N-NES
    

    Use

    Tags

    6xHIS - T7 tag (gene 10 leader) - Xpress (TM) tag…

    Expression

    Mammalian

    Mutation

    Target Thr residue in substrate domain mutated to…

    Promoter

    CMV

    Available since

    Availability

    Academic Institutions and Nonprofits only

    

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16. pcDNA3.1(+)_U6 tRNAPyl_CMV NESPylRS(AF)_IRES_eRF1(E55D)-HA

    Plasmid

    #182653

    Purpose

    Encodes codon-optimized AF mutant of M. mazei pyrrolysine (Pyl) tRNA synthetase fused to a nuclear export signal (NESPylRSAF),tRNACUAPyl & eRF1E55D used for amber codon suppression in mammalian cells

    Depositor

    Ivana Nikić-Spiegel

    Article

    Arsic et al Nat Commun. 2022 Jan 14;13(1):314. doi:

    Inserts

    codon-optimized Y306A/Y384F (AF) double mutant of Methanosarcina mazei-derived pyrrolysine (Pyl) tRNA synthetase
    PylT
    mutant eukaryotic release factor 1
    

    Use

    Tags

    HA tag and nuclear export signal (NES)

    Expression

    Mammalian

    Mutation

    E55D and Y306A/Y384F (AF) double mutant of Methan…

    Promoter

    CMV and U6

    Available since

    June 16, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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17. AAV-CAG-FLEX-NES-jGCaMP8s-WPRE

    Plasmid

    #186046

    Purpose

    AAV-mediated cytosolic expression of jGCaMP8s under the CAG promoter, Cre-dependent expression

    Depositor

    Loren Looger

    Insert

    NES-jGCaMP8s
    

    Use

    AAV

    Tags

    NES-6xHis

    Expression

    Mammalian

    Mutation

    Promoter

    CAG

    Available since

    Aug. 24, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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18. pDF0352 HvsCas7-11 with C-term Nes mammalian expression

    Plasmid

    #172516

    Purpose

    Encodes human codon optimized HvsCas7-11 protein with C-terminal NES tag for expression in mammalian cells

    Depositor

    Omar Abudayyeh, Jonathan Gootenberg

    Article

    Ozcan et al Nature. 2021 Sep 6. pii: 10.1038/s41586-

    Insert

    C-term Nes HvsCas7-11 mammalian expression
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Oct. 7, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. mCherry-NES-SspB-SOS2cat-P2A-iLIDcaax

    Plasmid

    #173873

    Purpose

    Mammalian expression of mCherry-NES-SspB-SOS2cat-P2A-iLIDcaax (opto-Ras)

    Depositor

    Takao Nakata

    Article

    Inaba et al J Biol Chem. 2021 Jan-Jun;296:100290. do

    Insert

    mCherry-NES-SspB-SOS2cat-P2A-iLIDcaax
    

    Use

    Tags

    C-terminal CAAX-box and mCherry

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Sept. 2, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pDONR_P2R-P3_R2-Turbo-NES-mVenus-STOP-L3

    Plasmid

    #127353

    Purpose

    gateway entry vector for making C-terminal TurboID-mVenus fusion with non-nuclear proteins

    Depositor

    Dominique Bergmann

    Article

    Mair et al Elife. 2019 Sep 19;8. pii: 47864. doi: 1

    Insert

    TurboID (BirA mutant)
    

    Use

    Gateway entry vector

    Tags

    GS linker, NES, V5, and mVenus

    Expression

    Mutation

    Q65P, I87V, R118S, E140K, Q141R, S150G, L151P, V1…

    Promoter

    no promoter

    Available since

    Sept. 18, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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