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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 1,003 results for: transfection

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Showing: 501 - 520 of 1003 results

  2. HA-IFITM3-F8-TAG

    Plasmid
    #122475
    Purpose
    expresses human IFITM3 with unnatural amino acid incorporated site specifically in response to the amber codon TAG in mammalian cells when co-transfected with tRNA synthetase/tRNA pair.
    Depositor
    Howard Hang
    Article
    Spence et al Nat Chem Biol. 2019 Jan 14. pii: 10.1038
    Insert
    IFITM3 (IFITM3 Human)
    Tags
    HA
    Expression
    Mammalian
    Mutation
    Changed Phenylalanine 8 to amber codon
    Available Since
    March 8, 2019
    Availability
    Academic Institutions and Nonprofits only
    122475-plasmid-map-sequence-id-237716

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  4. qTAG-N-Blast-ultraID-LMNB1

    Plasmid
    #207775
    Purpose
    Donor template for Blast-2A-ultraID insertion into the N-terminus of the LMNB1 locus. To be co-transfected with sgRNA plasmid px330-PITCh-LMNB1 Addgene #207770
    Depositor
    Laurence Pelletier
    Article
    Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318
    Insert
    LMNB1 Homology Arms flanking a Blast-ultraID Cassette (LMNB1 Human)
    Use
    CRISPR; Donor template
    Expression
    Mammalian
    Available Since
    Nov. 29, 2023
    Availability
    Industry, Academic Institutions, and Nonprofits
    207775-plasmid-map-sequence-id-416196

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  8. qTAG-C-moxGFP-Puro-MAPRE1

    Plasmid
    #207794
    Purpose
    Donor template to insert moxGFP-2A-Puro into the C-terminus of the MAPRE1 locus for growing microtubule tip visualization. To be co-transfected with sgRNA plasmid px330-PITCh-MAPRE1 Addgene #207793
    Depositor
    Laurence Pelletier
    Article
    Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318
    Insert
    MAPRE1 Homology Arms flanking a moxGFP-Puro Cassette (MAPRE1 Human)
    Use
    CRISPR; Donor template
    Expression
    Mammalian
    Available Since
    Dec. 1, 2023
    Availability
    Industry, Academic Institutions, and Nonprofits
    207794-plasmid-map-sequence-id-416380

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  9. qTAG-N-Puro-mScarlet-ACTB

    Plasmid
    #207754
    Purpose
    Donor template for Puro-2A-mScarlet insertion into the N-terminus of the ACTB locus for actin visualization. To be co-transfected with sgRNA plasmid px330-PITCh-ACTB Addgene #207748
    Depositor
    Laurence Pelletier
    Article
    Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318
    Insert
    ACTB Homology Arms flanking a Puro-mScarlet Cassette (ACTB Human)
    Use
    CRISPR; Donor template
    Expression
    Mammalian
    Available Since
    Nov. 16, 2023
    Availability
    Industry, Academic Institutions, and Nonprofits
    207754-plasmid-map-sequence-id-415028

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  11. qTAG-C-moxGFP-Puro-H3C2

    Plasmid
    #207783
    Purpose
    Donor template for moxGFP-2A-Puro insertion into the C-terminus of the H3C2 locus for nuclei visualization. To be co-transfected with sgRNA plasmid px330-PITCh-H3C2 Addgene #207780
    Depositor
    Laurence Pelletier
    Article
    Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318
    Insert
    H3C2 Homology Arms flanking a moxGFP-Puro Cassette (H3C2 Human)
    Use
    CRISPR; Donor template
    Expression
    Mammalian
    Available Since
    Nov. 29, 2023
    Availability
    Industry, Academic Institutions, and Nonprofits
    207783-plasmid-map-sequence-id-416185

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  12. pSBL_DK202

    Plasmid
    #156426
    Purpose
    Mammalian expression of 3a delta N(aa 42-275)-EGFP with CMV promoter, can be used for transfection or used to generate bacmids for baculoviral infection of mammalian cells
    Depositor
    Stephen Brohawn
    Article
    Kern et al bioRxiv [Preprint]. 2021 Jan 26:2020.06.
    Insert
    SARS-CoV-2 3a protein (aa 42-275)
    Tags
    EGFP-His
    Expression
    Mammalian
    Mutation
    aa 42-275
    Promoter
    CMV
    Available Since
    July 21, 2020
    Availability
    Academic Institutions and Nonprofits only
    156426-plasmid-map-sequence-id-306303

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  14. qTAG-N-Blast-sTagRFP-TUBA1B

    Plasmid
    #207768
    Purpose
    Donor template for Blast-2A-sTagRFP insertion into the N-terminus of the TUBA1B locus for tubulin visualization. To be co-transfected with sgRNA plasmid px330-PITCh-TUBA1B Addgene #207763
    Depositor
    Laurence Pelletier
    Article
    Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318
    Insert
    TUBA1B Homology Arms flanking a Blast-sTagRFP Cassette (TUBA1B Human)
    Use
    CRISPR; Donor template
    Expression
    Mammalian
    Available Since
    Nov. 15, 2023
    Availability
    Industry, Academic Institutions, and Nonprofits
    207768-plasmid-map-sequence-id-413803

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  15. HA-IFITM3-F8-TAG-C72A

    Plasmid
    #122477
    Purpose
    expresses human IFITM3-C72A with unnatural amino acid incorporated site specifically in response to the amber codon TAG in mammalian cells when co-transfected with tRNA synthetase/tRNA pair.
    Depositor
    Howard Hang
    Article
    Spence et al Nat Chem Biol. 2019 Jan 14. pii: 10.1038
    Insert
    IFITM3 (IFITM3 Human)
    Tags
    HA
    Expression
    Mammalian
    Mutation
    Changed Phenylalanine 8 to amber codon, cysteine …
    Available Since
    March 8, 2019
    Availability
    Academic Institutions and Nonprofits only
    122477-plasmid-map-sequence-id-237728

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  17. IFITM3-N21del-V93-TAG

    Plasmid
    #122480
    Purpose
    expresses human IFITM3-N21del with unnatural amino acid incorporated site specifically in response to the amber codon TAG in mammalian cells when co-transfected with tRNA synthetase/tRNA pair.
    Depositor
    Howard Hang
    Article
    Spence et al Nat Chem Biol. 2019 Jan 14. pii: 10.1038
    Insert
    IFITM3 (IFITM3 Human)
    Expression
    Mammalian
    Mutation
    Changed Valine 93 to amber codon and deleted amin…
    Available Since
    March 14, 2019
    Availability
    Academic Institutions and Nonprofits only
    122480-plasmid-map-sequence-id-237764

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  20. HA-IFITM3-F8-TAG-C105A

    Plasmid
    #122478
    Purpose
    expresses human IFITM3-C105A with unnatural amino acid incorporated site specifically in response to the amber codon TAG in mammalian cells when co-transfected with tRNA synthetase/tRNA pair.
    Depositor
    Howard Hang
    Article
    Spence et al Nat Chem Biol. 2019 Jan 14. pii: 10.1038
    Insert
    IFITM3 (IFITM3 Human)
    Tags
    HA
    Expression
    Mammalian
    Mutation
    Changed Phenylalanine 8 to amber codon, cysteine …
    Available Since
    March 8, 2019
    Availability
    Academic Institutions and Nonprofits only
    122478-plasmid-map-sequence-id-237731

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Showing: 501 - 520 of 1003 results