We narrowed to 8,929 results for: c myc plasmid

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pCMVtrunc Affimer6-sfCherry2ΔN12

Plasmid

#231551

Purpose

Mammalian expression of actin-binding Affimer6 fused to N-terminally truncated superfolder Cherry2, for actin filament organization measurements using fluorescence polarization microscopy

Depositor

Manos Mavrakis

Article

Martins et al Cell. 2025 Apr 1:S0092-8674(25)00276-4.

Insert

Affimer6

Tags

sfCherry2

Expression

Mammalian

Promoter

CMVtrunc

Available Since

Jan. 30, 2025

Availability

Academic Institutions and Nonprofits only

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pCMVtrunc Affimer6-msfGFPΔN12

Plasmid

#231550

Purpose

Mammalian expression of actin-binding Affimer6 fused to N-terminally truncated monomeric superfolder GFP, for actin filament organization measurements using fluorescence polarization microscopy

Depositor

Manos Mavrakis

Article

Martins et al Cell. 2025 Apr 1:S0092-8674(25)00276-4.

Insert

Affimer6

Tags

msfGFP

Expression

Mammalian

Promoter

CMVtrunc

Available Since

Jan. 30, 2025

Availability

Academic Institutions and Nonprofits only

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pLenti TMEM30A

Plasmid

#171789

Purpose

transfer plasmid for lentiviral vector production expressing TMEM30A

Depositor

Veerle Baekelandt

Article

Martin et al Acta Neuropathol. 2020 Jun;139(6):1001-1

Insert

TMEM30A (TMEM30A Human)

Use

Lentiviral

Tags

6X His + Flag

Promoter

CMV

Available Since

July 6, 2021

Availability

Academic Institutions and Nonprofits only

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LCV2_mClover3_LacZ

Plasmid

#155103

Purpose

lentiviral plasmid expressing mClover3, Cas9 and a gRNA targeting LacZ

Depositor

Jason Moffat

Article

Aregger et al Nat Metab. 2020 Jun;2(6):499-513. doi: 1

Insert

LacZ_sgRNA_2

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

U6 promoter

Available Since

Aug. 7, 2020

Availability

Academic Institutions and Nonprofits only

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LCV2_mCherry_LacZ_sgRNA

Plasmid

#155108

Purpose

lentiviral plasmid expressing mCherry, Cas9 and a gRNA targeting LacZ

Depositor

Jason Moffat

Article

Aregger et al Nat Metab. 2020 Jun;2(6):499-513. doi: 1

Insert

LacZ_sgRNA_2

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

U6 promoter

Available Since

Aug. 7, 2020

Availability

Academic Institutions and Nonprofits only

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LCV2_mCherry_Luciferase_sgRNA

Plasmid

#155109

Purpose

lentiviral plasmid expressing mCherry, Cas9 and a gRNA targeting Luciferase

Depositor

Jason Moffat

Article

Aregger et al Nat Metab. 2020 Jun;2(6):499-513. doi: 1

Insert

Luciferase_sgRNA

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

U6 promoter

Available Since

Aug. 7, 2020

Availability

Academic Institutions and Nonprofits only

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psgRNAv1-empty

Plasmid

#171611

Purpose

sgRNA_v1 plasmid for AsCas12f1 in bacteria

Depositor

Quanjiang Ji

Article

Wu et al Nat Chem Biol. 2021 Sep 2. pii: 10.1038/

Insert

AsCas12f1_sgRNA_1

Expression

Bacterial

Available Since

Sept. 10, 2021

Availability

Academic Institutions and Nonprofits only

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pscALPSpuro-MjACE2 (Pangolin)

Plasmid

#158084

Purpose

Expresses pangolin ACE2 in mammalian cells

Depositor

Jeremy Luban

Article

Yurkovetskiy et al Cell. 2020 Sep 15. pii: S0092-8674(20)31

Insert

Pangolin ACE2 (ACE2 )

Use

Lentiviral

Expression

Mammalian

Available Since

Oct. 28, 2020

Availability

Academic Institutions and Nonprofits only

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pscALPSpuro-HsACE2 (human)

Plasmid

#158081

Purpose

Expresses human ACE2 in mammalian cells

Depositor

Jeremy Luban

Article

Yurkovetskiy et al Cell. 2020 Sep 15. pii: S0092-8674(20)31

Insert

Human ACE2 (ACE2 Human)

Use

Lentiviral

Expression

Mammalian

Available Since

Oct. 28, 2020

Availability

Academic Institutions and Nonprofits only

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pET28b-SpG-His

Plasmid

#179318

Purpose

Plasmid for bacterial expression and purification of SpG-Cas9

Depositor

Julián Cerón, Miguel Angel Moreno-Mateos

Article

Vicencio et al Nat Commun. 2022 May 12;13(1):2601. doi:

Insert

Human codon-optimized Cas9 variant SpG

Use

CRISPR

Tags

6xHis and SV40-NLS

Expression

Bacterial

Mutation

SpG=D1135L/S1136W/G1218K/E1219Q/ R1335Q/T1337R

Available Since

May 12, 2022

Availability

Academic Institutions and Nonprofits only

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pF CAG BFP2 IRES neo

Plasmid

#108175

Purpose

Expresses a blue fluorescent protein (BFP2) in mammalian cells along with conferring resistance to G418

Depositor

Brett Stringer

Article

Stringer et al Sci Rep. 2019 Mar 20;9(1):4902. doi: 10.

Insert

Blue fluorescent protein (BFP2)

Use

Lentiviral; Fluorescent protein

Expression

Mammalian

Promoter

CAG

Available Since

Aug. 10, 2018

Availability

Academic Institutions and Nonprofits only

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pscALPSpuro-SsACE2 (Pig)

Plasmid

#158085

Purpose

Expresses pig ACE2 in mammalian cells

Depositor

Jeremy Luban

Article

Yurkovetskiy et al Cell. 2020 Sep 15. pii: S0092-8674(20)31

Insert

Pig ACE2 (ACE2 Sus scrofa)

Use

Lentiviral

Expression

Mammalian

Available Since

Oct. 28, 2020

Availability

Academic Institutions and Nonprofits only

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pOT_4 - lenti-EFS-FMC6.3-BBz-2A-puro-2A-LTBR

Plasmid

#181973

Purpose

Expresses anti-CD19 CAR (with 4-1BB signaling domain) linked to puromycin resistance via P2A and to human LTBR via T2A for lentiviral delivery.

Depositor

Neville Sanjana

Article

Legut et al Nature. 2022 Mar;603(7902):728-735. doi:

Insert

FMC6.3-BBz CAR; LTBR (LTBR Human, Synthetic)

Use

Lentiviral

Available Since

April 25, 2022

Availability

Academic Institutions and Nonprofits only

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pscALPSpuro-ClACE2 (Dog)

Plasmid

#158083

Purpose

Expresses dog ACE2 in mammalian cells

Depositor

Jeremy Luban

Article

Yurkovetskiy et al Cell. 2020 Sep 15. pii: S0092-8674(20)31

Insert

Dog ACE2 (ACE2 )

Use

Lentiviral

Expression

Mammalian

Available Since

Oct. 28, 2020

Availability

Academic Institutions and Nonprofits only

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pscALPSpuro-RnACE2 (Rat)

Plasmid

#158086

Purpose

Expresses rat ACE2 in mammalian cells

Depositor

Jeremy Luban

Article

Yurkovetskiy et al Cell. 2020 Sep 15. pii: S0092-8674(20)31

Insert

Rat ACE2 (Ace2 Rat)

Use

Lentiviral

Expression

Mammalian

Available Since

June 15, 2021

Availability

Academic Institutions and Nonprofits only

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pOT_3 - lenti-EFS-FMC6.3-28z-2A-puro-2A-LTBR

Plasmid

#181972

Purpose

Expresses anti-CD19 CAR (with CD28 signaling domain) linked to puromycin resistance via P2A and to human LTBR via T2A for lentiviral delivery.

Depositor

Neville Sanjana

Article

Legut et al Nature. 2022 Mar;603(7902):728-735. doi:

Insert

FMC6.3-28z CAR; LTBR (LTBR Human, Synthetic)

Use

Lentiviral

Available Since

April 25, 2022

Availability

Academic Institutions and Nonprofits only

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pCR2.1-KRAS-G12V

Plasmid

#129091

Purpose

KI KRAS G12V mutation into human KRAS locus

Depositor

Toshiro Sato

Article

Matano et al Nat Med. 2015 Mar;21(3):256-62. doi: 10.

Insert

KRAS(G12V) (KRAS Human)

Use

Homologous recombination donor plasmid

Mutation

G12V

Available Since

Sept. 30, 2019

Availability

Academic Institutions and Nonprofits only

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pCW57-Cx32D178Y-IRES-GCaMP6s

Plasmid

#216796

Purpose

Inducible bicistronic lentiviral plasmid for the simultaneous expression of the D178Y mutant form of Connexin 32 and cytosolic GCaMP6s

Depositor

Fabio Mammano

Article

Tettey-Matey et al Cell Commun Signal. 2024 Dec 5;22(1):589

Insert

Cx32D178Y-IRES-GCaMP6s (GJB1 Human)

Use

Lentiviral

Mutation

D178Y mutant form of Connexin 32

Available Since

April 19, 2024

Availability

Academic Institutions and Nonprofits only

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pET28a-SUMO-AsCas12f

Plasmid

#171612

Purpose

AsCas12f1 heterologous expression plasmid

Depositor

Quanjiang Ji

Article

Wu et al Nat Chem Biol. 2021 Sep 2. pii: 10.1038/

Insert

AsCas12f1

Expression

Bacterial

Available Since

Sept. 14, 2021

Availability

Academic Institutions and Nonprofits only

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pET28b-SpRY-His

Plasmid

#179320

Purpose

Plasmid for bacterial expression and purification of SpRY-Cas9

Depositor

Julián Cerón, Miguel Angel Moreno-Mateos

Article

Vicencio et al Nat Commun. 2022 May 12;13(1):2601. doi:

Insert

Human codon-optimized Cas9 variant SpRY

Use

CRISPR

Tags

6xHis and SV40-NLS

Expression

Bacterial

Mutation

SpRY=A61R/L1111R/D1135L/S1136W/G1218K/E1219Q/ N13…

Available Since

May 12, 2022

Availability

Academic Institutions and Nonprofits only

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