We narrowed to 5,942 results for: Chia
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Plasmid#66073PurposeThe burden monitor was cloned inside plasmid pAH63 [Haldimann2001]; the genome integration of the system allows the measurement of the burden imposed by synthetic constructs to Escherichia coli cellsDepositorInsertGFP
Available SinceDec. 21, 2015AvailabilityAcademic Institutions and Nonprofits only -
pAT253
Plasmid#40943DepositorInsertGFP-LacI**
TagsGFP(S65T, V163A) and SV40 NLSExpressionYeastMutationLacI**/LacI2G(4 mutations) (see comment below); l…PromoterHis3 promoter fragmentAvailable SinceFeb. 12, 2015AvailabilityAcademic Institutions and Nonprofits only -
pET22b_PstS_2
Plasmid#78199PurposeExpression of double-cysteine variant of mature phosphate binding protein, PstS, as scaffold for phosphate biosensorDepositorInsertpstS (A17C,A197C)
ExpressionBacterialMutationA17C; A197C (please see depositor comment below)PromoterT7Available SinceJuly 11, 2016AvailabilityAcademic Institutions and Nonprofits only -
prDNA spacer-LacOx50-20xMS2-Hyg
Plasmid#210219PurposeIntegrates 50xLacO repeats and 24xMS2 repeats with a hygromycin drug selection marker into the rDNA spacer.DepositorInserts50xLacO repeats derived from: PMID: 12864855.
24xMS2 repeats derived from Addgene plasmid # 31865
Available SinceDec. 8, 2023AvailabilityAcademic Institutions and Nonprofits only -
pHL 1032
Plasmid#52997PurposepLtetO-1:RhyB, pCon:ompC::mCherry, pLlacO-1:sodB::gfpDepositorInsertsRhyB sRNA
ompC
sodB
UseSynthetic BiologyTagsgfp and mCherryExpressionBacterialMutationcontains −56 to +141 bp relative to start codon a…PromoterpCon, pLlacO-1, and pLtetO-1Available SinceMay 16, 2014AvailabilityAcademic Institutions and Nonprofits only -
pCJR01
Plasmid#187888PurposeEscherichia coli plasmid with cloned regions of homology to Campylobacter jejuni chromosome flanking a NotI restriction site. pCJR01 functions as a suicide vector for integrating cloned NotI DNA.DepositorInsertDNA insert has homology to a rRNA region of the Campylobacter jejuni chromosome with an added central NotI restriction site
ExpressionBacterialPromoternoneAvailable SinceAug. 15, 2022AvailabilityAcademic Institutions and Nonprofits only -
pET21-Flavidin
Plasmid#89881PurposeFluorophore-friendly streptavidin that can improve fluorescent brightness and has unimpaired biotin binding after dye labelingDepositorInsertStreptavidin-K80R-K121R-K132R-K134R-Glutamate_Tag
Tags6 glutamate tag (C terminal on insert)ExpressionBacterialMutationK80R, K121R, K132R, K134RAvailable SinceAug. 9, 2017AvailabilityAcademic Institutions and Nonprofits only -
pET41a iGluu
Plasmid#119834PurposeProduction of recombinant iGluSnFR S72T ultrafast variant, a single-wavelength glutamate indicator, in E. coliDepositorInsertiGluu
TagsGSTExpressionBacterialMutationS72TPromoterT7Available SinceApril 1, 2019AvailabilityAcademic Institutions and Nonprofits only -
pTS2355_Tier3(PB)-iRFP_PuroR
Plasmid#169654PurposeTier-3 vector for stable stable integration of up to three cassettes via Piggy Bac transposase including a iRFP670 marker and PuroR resistance gene (5'ITR-A1-pA::A2-pA::PRPBSA-iRFP670-p2A-PuroR-pA-3'ITR)DepositorInsertPRPBSA-driven iRFP670 and PuroR expression
ExpressionMammalianPromoterPRPBSAAvailable SinceJune 28, 2022AvailabilityAcademic Institutions and Nonprofits only -
pYFJ16-LplA(W37V) (for E. Coli expression)
Plasmid#34838DepositorInsertLplA W37V (lplA E. Coli)
TagsHis TagExpressionBacterialMutationMutated Trp37 to ValPromoterT5 promoterAvailable SinceMay 16, 2012AvailabilityAcademic Institutions and Nonprofits only -
pAAV-CAG-tTA
Plasmid#193338PurposeAAV-mediated expression of tTA under the CAG promoter in mammalian cellsDepositorInserttTA2
UseAAVExpressionMammalianPromoterCAGAvailable SinceAug. 22, 2023AvailabilityAcademic Institutions and Nonprofits only -
pLaNGo11-7
Plasmid#126055PurposeA self-assembling split-GFP based Golden Gate vector to determine protein targeting to plastids in plant cellDepositorInsertschloroplastic FNR transit peptide
ccdB cassette (BsaI)
GFP11x7 (5 aa linker)
UseBinaryTagsGFP1-10ExpressionPlantAvailable SinceJune 27, 2019AvailabilityAcademic Institutions and Nonprofits only -
pXD70LacZ6-LacI-Ptac(LacO)-LacZ
Plasmid#191621PurposeE. coli - Eggerthella lenta shuttle plasmid (KanR), IPTG-inducible promoter-lacZ, engineered from pMAL-c2XDepositorInsertlacZ
ExpressionBacterialAvailable SinceJan. 5, 2023AvailabilityAcademic Institutions and Nonprofits only -
pSLS.405
Plasmid#187203PurposeExpresses wild-type retron Eco1 ncRNA + Eco1 RT, and Cas1 + Cas2DepositorInsertsRetron Eco1 ncRNA (wildtype)
Cas1 + Cas2
UseSynthetic BiologyExpressionBacterialPromoterT7/LacAvailable SinceSept. 28, 2022AvailabilityAcademic Institutions and Nonprofits only -
pJB02
Plasmid#111959PurposeExpresses GST-GFP-DHFR; construct generated for pull-down and localization experimentsDepositorInsertsmEGFP
eDHFR
TagsDHFRExpressionBacterialMutationeDHFR;residues 2-159 and enhanced GFP with monome…PromoterT7Available SinceAug. 22, 2018AvailabilityAcademic Institutions and Nonprofits only -
pBEL2319
Plasmid#195697PurposeCryo-EM of 6xHis-Foldon-(Gly-Ser-Linker)-MlaCDepositorInsertFoldon-MlaC
ExpressionBacterialMutation6xHis-Foldon-(Gly-Ser-Linker)-MlaCAvailable SinceMay 2, 2023AvailabilityAcademic Institutions and Nonprofits only -
pMS17
Plasmid#127088PurposeIntegrating vector containing a tetracycline inducible promoter for use in Streptomyces sp.DepositorInsertstcp830
TetRiS
terminator mmrt
Terminator fdt
UseStreptomyce integration vector derived from phic3…Available SinceJuly 10, 2019AvailabilityAcademic Institutions and Nonprofits only -
pBRα-σ70 D581G
Plasmid#53732PurposePlpp/PlacUV5- directed synthesis of the alpha NTD fused to E. coli σ70 region 4. Used as two-hybrid control. For cloning purposes, see Addgene plasmid 53734.DepositorInsertRNAP alpha NTD (aa 1-248) fused to E. coli sigma 70 (aa 528-613)
ExpressionBacterialMutationThe sigma 70 moiety carries the D581G substitutionPromoterlpp/lacUV5 (tandem promoter)Available SinceJune 4, 2014AvailabilityAcademic Institutions and Nonprofits only -
pMG343
Plasmid#176183Purposepreassembled yeast MoClo level-1 backbone with toxic gene dropout cassette (ccdB and TPK2) and URA3 markerDepositorInsertTPK2+ccdB dropout cassette
ExpressionYeastAvailable SinceFeb. 4, 2022AvailabilityAcademic Institutions and Nonprofits only -
LacZ-5BoxB in pcDNA3.1+
Plasmid#29729DepositorInsertLacZ-5BoxB
ExpressionMammalianAvailable SinceMay 23, 2011AvailabilityAcademic Institutions and Nonprofits only