We narrowed to 29,660 results for: REP

1. LV-hHOXA13

   Plasmid

   #135561

   Purpose

   Express hHOXA13 and EGFP in mammalian cells

   Depositor

   Jose Teixeira

   Article

   George et al Cell Rep. 2019 Dec 17;29(12):4069-4085.e

   Insert

   Homeobox A13 (HOXA13 Human)
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CBH

   Available since

   Jan. 9, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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2. pelB-MBP-P13-ss

   Plasmid

   #137065

   Purpose

   E. coli expression clone (T7lac promoter) for mature P13 (aa 22-179) from B. burgdorferi with N-terminal PelB signal peptide + His10 + mature maltose binding protein + TEV protease cleavage

   Depositor

   Debra Hansen

   Article

   Robertson et al Sci Rep. 2019 Nov 26;9(1):17606. doi: 10

   Insert

   AAC66426.1 (BB_0034 Borrelia burgdorferi B31)
   

   Use

   Tags

   PelB signal peptide + His10 + maltose binding pro…

   Expression

   Bacterial

   Mutation

   mature P13: lacks the P13 signal peptide

   Promoter

   T7lac

   Available since

   April 17, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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3. pcmv3-STING-C1471S-FLAG

   Plasmid

   #198188

   Purpose

   Mammalian expression of FLAG-tagged mouse STING1 C1471S

   Depositor

   Yun Zhao

   Article

   Li et al Cell Rep. 2023 Mar 28;42(3):112145. doi:

   Insert

   TMEM173 (Sting1 Mouse)
   

   Use

   Tags

   FLAG

   Expression

   Mammalian

   Mutation

   The cysteine in STING at position 147 mutates to …

   Promoter

   CMV

   Available since

   April 7, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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4. pCDNA4TO R124A K125A CoV-2 nsp1 3xFLAG

   Plasmid

   #176059

   Purpose

   Expressing R124A K125A CoV2 nsp1 in mammalian cells. This contains a C-terminal 3xFLAG tag fusion to nsp1.

   Depositor

   Britt Glaunsinger

   Article

   Mendez et al Cell Rep. 2021 Oct 19;37(3):109841. doi:

   Insert

   CoV-2 nsp1 R124A/K125A (ORF1ab SARS-CoV-2)
   

   Use

   Tags

   3xFLAG

   Expression

   Mammalian

   Mutation

   R124A and K125A

   Promoter

   CMV

   Available since

   Oct. 14, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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5. pPN458

   Plasmid

   #137876

   Purpose

   PiggyBac vector for expression of 1x gRNA targeting TCF4 for CRISPRa;mRFP-T2A-BlasticidinR

   Depositor

   Lindy Barrett

   Article

   Hazelbaker et al Sci Rep. 2020 Jan 20;10(1):635. doi: 10.

   Insert

   TCF4 (TCF4 Human)
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Feb. 10, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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6. pRSFDuet-1_VNp-LZ_VNp-LZ-mNeongreen-hGH

   Plasmid

   #182425

   Purpose

   Bacterial expression of Vesicle Nucleating peptide-Leucine Zipper and Vesicle Nucleating peptide-Leucine Zipper-mNeongreen-human growth hormone fusion proteins

   Depositor

   Dan Mulvihill

   Article

   Eastwood et al Cell Rep Methods. 2023 Feb 2;3(2):100396

   Inserts

   VNp-LZ-mNeongreen-hGH (GH1 Human)
   VNp-LZ
   

   Use

   Tags

   Vesicle Nucleating peptide (VNp) and Vesicle Nucl…

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   Feb. 3, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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7. PB-PEmax SpG

   Plasmid

   #187895

   Purpose

   All-in-one prime editor piggyBac transposon with PEmax, SpG variant

   Depositor

   Tobias Cantz

   Article

   Eggenschwiler et al Sci Rep. 2021 Nov 12;11(1):22154. doi: 1

   Insert

   SpCas9_H840A_KK_SpG-MMLV-RT_co-P2A-PAC_dTK
   

   Use

   CRISPR and Synthetic Biology; Piggybac transposon

   Tags

   BPSV40 NLS and c-Myc NLS

   Expression

   Mammalian

   Mutation

   Promoter

   CAG

   Available since

   Aug. 31, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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8. p-mCherry-C1-Phobos_CA

   Plasmid

   #98169

   Purpose

   slow cycling (open for minutes) step-function artificial anion conducting channelrhodopsin (aACR). High light sensitivity. Activation with blue light, inactivation max with 580 nm (accelarates closure to ms). Codon optimized for mammalian expression.

   Depositor

   Peter Hegemann

   Article

   Wietek et al Sci Rep. 2017 Nov 2;7(1):14957. doi: 10.

   Insert

   Synthetic construct Phobos_C128A gene
   

   Use

   Tags

   mCherry

   Expression

   Mammalian

   Mutation

   T59S, E83N, E90Q, E101S, V117R, E123S, C128A, T15…

   Promoter

   CMV (+enhancer)

   Available since

   July 14, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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9. pSBbi-RP-CoV2/stSpike-puro

   Plasmid

   #161794

   Purpose

   Transposon-based SARS-CoV-2 secretable trimeric Spike stable expression vector for protein production

   Depositor

   Jorge Luis Arias-Arias

   Article

   Arias-Arias et al Biotechnol Rep (Amst). 2023 Mar;37:e0078

   Insert

   SARS-CoV-2 secretable trimeric Spike
   

   Use

   Transposon

   Tags

   6xHis and dTomato

   Expression

   Mammalian

   Mutation

   Promoter

   EF1a/RPBSA

   Available since

   Aug. 10, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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10. pRetroX-TRE3G_FLAG-CRTC1/MAML2_PGK-GpNLuc

    Plasmid

    #154265

    Purpose

    Dox/Tet-inducible expression of the CRTC1-MAML2 fusion oncogene in mammalian cells.

    Depositor

    Antonio Amelio

    Article

    Musicant et al Cell Rep. 2021 Feb 23;34(8):108768. doi:

    Insert

    FLAG-CRTC1/MAML2 fusion oncogene
    

    Use

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    Promoter

    TRE3G

    Available since

    March 8, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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11. nNOS/NOS1 scFv [L121/133]

    Plasmid

    #206759

    Purpose

    Mammalian Expression of nNOS/NOS1 scFV. Derived from hybridoma L121/133 scFv.

    Depositor

    James Trimmer

    Article

    Mitchell et al Sci Rep. 2023 Sep 27;13(1):16200. doi: 1

    Insert

    nNOS/NOS1 (Homo sapiens) recombinant scFV (NOS1 Mouse)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Oct. 23, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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12. PB-PE xCas9

    Plasmid

    #172652

    Purpose

    All-in-one prime editor piggyBac transposon (xCas9)

    Depositor

    Tobias Cantz

    Article

    Eggenschwiler et al Sci Rep. 2021 Nov 12;11(1):22154. doi: 1

    Insert

    xCas9_H840A-PE2-MMLV-RT(dBB)-P2A-PAC_dTK(dBB)
    

    Use

    CRISPR and Synthetic Biology; Piggybac transposon

    Tags

    SV40 NLS

    Expression

    Mammalian

    Mutation

    A262T, R324L, S409I, E480K, E543D, M694I, E1219V

    Promoter

    Available since

    Nov. 16, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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13. pAAV-VTKS6

    Plasmid

    #170857

    Purpose

    AAV backbone for Cre- and Flp-dependent transgene expression (CRE-OFF / FLP-ON) of any transgene in neurons under the control of the hSyn1 promoter.

    Depositor

    Jordane Dimidschstein

    Article

    Pouchelon et al Cell Rep Methods. 2022 May 26;2(6):10022

    Type

    Empty backbone

    Use

    AAV

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    May 26, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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14. Anti-GFP [N86/38R-1]

    Plasmid

    #206719

    Purpose

    Mammalian Expression Plasmid of anti-GFP (Aequorea victoria). Derived from hybridoma N86/38-1.

    Depositor

    James Trimmer

    Article

    Mitchell et al Sci Rep. 2023 Sep 27;13(1):16200. doi: 1

    Insert

    anti-GFP (Aequorea victoria) recombinant Mouse monoclonal antibody
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Dual CMV

    Available since

    July 3, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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15. pLKO.5 shETV4-B

    Plasmid

    #74976

    Purpose

    shETV4 shRNA

    Depositor

    William Hahn

    Article

    Wang et al Cell Rep. 2017 Feb 7;18(6):1543-1557. do

    Insert

    ETV4 (ETV4 Human)
    

    Use

    RNAi

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    May 10, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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16. pcmv3-STING-C64S,C65S-FLAG

    Plasmid

    #198186

    Purpose

    Mammalian expression of FLAG-tagged mouse STING1 C64S, C65S

    Depositor

    Yun Zhao

    Article

    Li et al Cell Rep. 2023 Mar 28;42(3):112145. doi:

    Insert

    TMEM173 (Sting1 Mouse)
    

    Use

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    The cysteine in STING at position 64/65 mutates t…

    Promoter

    CMV

    Available since

    April 7, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. VAPA/B scFv [N479/107]

    Plasmid

    #206742

    Purpose

    Mammalian Expression of VAPA/B scFV. Derived from hybridoma N479/107 scFv.

    Depositor

    James Trimmer

    Article

    Mitchell et al Sci Rep. 2023 Sep 27;13(1):16200. doi: 1

    Insert

    VAPA/B (Rattus norvegicus) recombinant scFV (Vapa Mouse)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Oct. 23, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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18. pcDNA 3.3 ss-V5-hFZD4 (aa 37-537)

    Plasmid

    #115787

    Purpose

    Expresses human FZD4 with N-terminal V5-tag in mammalian cells, contains artificial signal sequence upstream of tag.

    Depositor

    Harald Junge

    Article

    Lai et al Cell Rep. 2017 Jun 27;19(13):2809-2822.

    Insert

    FZD4 (FZD4 Human)
    

    Use

    Tags

    V5

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Oct. 1, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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19. pLV-tetO-HA-GFI1B

    Plasmid

    #125599

    Purpose

    doxycycline-inducible expression of human GFI1B (HA tagged) in mammalian cells

    Depositor

    Filipe Pereira

    Article

    Gomes et al Cell Rep. 2018 Dec 4;25(10):2821-2835.e7

    Insert

    Growth Factor Independent 1B transcriptional repressor (GFI1B Human)
    

    Use

    Lentiviral

    Tags

    HA tag

    Expression

    Mammalian

    Mutation

    Promoter

    TRE-mCMV

    Available since

    June 12, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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20. pAAV-VTKS3

    Plasmid

    #170854

    Purpose

    AAV backbone for Flp-dependent transgene expression of any transgene in neurons under the control of the hSyn1 promoter.

    Depositor

    Jordane Dimidschstein

    Article

    Pouchelon et al Cell Rep Methods. 2022 May 26;2(6):10022

    Type

    Empty backbone

    Use

    AAV

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    May 26, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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