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pcDNA3-MTS-KD-c-Src-FLAG

Plasmid

#44653

Depositor

Yoshimi Homma

Article

Ogura et al Biochem J. 2012 Oct 15;447(2):281-9.

Insert

v-src sarcoma (Schmidt-Ruppin A-2) viral oncogene homolog (avian) (SRC) (SRC Human)

Tags

FLAG tag and mitochondria-targeting sequence (MTS…

Expression

Mammalian

Mutation

changed lysine 298 to methionine

Promoter

CMV promoter

Available Since

May 22, 2013

Availability

Academic Institutions and Nonprofits only

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pEX-YFP-STIM1-deltaK

Plasmid

#18861

Depositor

Tobias Meyer

Article

Liou et al Proc Natl Acad Sci U S A. 2007 May 29. 1

Insert

STIM1 (STIM1 Human)

Tags

YFP

Expression

Mammalian

Mutation

Stop codon inserted at amino acid postion 671 of …

Available Since

Oct. 15, 2008

Availability

Academic Institutions and Nonprofits only

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pOT_5 - lenti-EFS-FMC6.3-28z-2A-puro-2A-tNGFR

Plasmid

#181974

Purpose

Expresses anti-CD19 CAR (with CD28 signaling domain) linked to puromycin resistance via P2A and to human truncated NGFR via T2A for lentiviral delivery.

Depositor

Neville Sanjana

Article

Legut et al Nature. 2022 Mar;603(7902):728-735. doi:

Insert

FMC6.3-28z CAR; tNGFR (NGFR Human, Synthetic)

Use

Lentiviral

Mutation

NGFR truncation: aa 11-276

Available Since

April 25, 2022

Availability

Academic Institutions and Nonprofits only

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pEF5-FRT-V5-DEST-GFP-Kif26b

Plasmid

#102862

Purpose

Expresses GFP-Kif26b in mammalian cells

Depositor

Henry Ho

Article

Susman et al Elife. 2017 Sep 8;6. pii: e26509. doi: 1

Insert

Mouse Kif26b (Kif26b Mouse)

Tags

GFP

Expression

Mammalian

Promoter

EF1A

Available Since

Dec. 8, 2017

Availability

Academic Institutions and Nonprofits only

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pcDNA3-Delta(Pro-MP)ADAM17-HA

Plasmid

#65221

Purpose

Expression of HA tagged ADAM17 lacking prodomain and metalloprotease domain in mammalian cells

Depositor

Axel Ullrich

Article

Gschwind et al EMBO J. 2003 May 15;22(10):2411-21.

Insert

Delta(Pro-MP)ADAM17 (ADAM17 Human)

Tags

HA

Expression

Mammalian

Mutation

deleted AA 18-473

Promoter

CMV

Available Since

Nov. 7, 2016

Availability

Academic Institutions and Nonprofits only

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pCDH_Str-KDEL_ST-SBP-EGFP

Plasmid

#65267

Purpose

synchronize trafficking of ST from the ER (RUSH system)

Depositor

Franck Perez

Article

Boncompain et al Nat Methods. 2012 Mar 11;9(5):493-8. doi

Insert

Str-KDEL and truncated sialyltransferase (ST6GAL1 Human)

Use

Lentiviral

Tags

EGFP and Streptavidin Binding Protein (SBP)

Mutation

contains only amino acids 1 to 43 of ST

Promoter

CMV

Available Since

Aug. 3, 2015

Availability

Academic Institutions and Nonprofits only

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mCardinal-Farnesyl-5

Plasmid

#56159

Purpose

Localization: Plasma Membrane, Excitation: 604, Emission: 659

Depositor

Michael Davidson

Article

Chu et al Nat Methods. 2014 Mar 16. doi: 10.1038/n

Insert

Farnesyl (HRAS Human)

Tags

mCardinal

Expression

Mammalian

Mutation

encodes final 20 aa of NM_001130442.1

Promoter

CMV

Available Since

Feb. 27, 2015

Availability

Industry, Academic Institutions, and Nonprofits
pCAG-Noggin

Plasmid

#163586

Purpose

Expresses Noggin in mammalian cells

Depositor

Takeshi Imai

Article

Aihara et al Cell Rep. 2021 Jun 22;35(12):109276. doi

Insert

Nog (Nog Mouse)

Expression

Mammalian

Available Since

Aug. 20, 2021

Availability

Academic Institutions and Nonprofits only

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pAV U6+27

Plasmid

#25709

Purpose

for nuclear expression of small RNAs in mammalian cells

Depositor

David Engelke

Article

Good et al Gene Ther. 1997 Jan . 4(1):45-54.

Insert

U6 snRNP (LSM2 Human)

Expression

Mammalian

Available Since

June 24, 2010

Availability

Academic Institutions and Nonprofits only

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PCTAIRE-1-HA

Plasmid

#1999

Depositor

Sander van den Heuvel

Article

van den Heuvel et al Science 1993 Dec 24;262(5142):2050-4.

Insert

PCTAIRE-1 (CDK16 Human)

Tags

HA

Expression

Mammalian

Available Since

Jan. 24, 2006

Availability

Academic Institutions and Nonprofits only
pCAG-R2Tg_opt-3xFlag

Plasmid

#223251

Purpose

Mammalian expression of C-terminally Flag-tagged optimized R2Tg protein

Depositor

Wei Li

Article

Chen et al Cell. 2024 Jul 1:S0092-8674(24)00694-9.

Insert

R2Tg protein_opt

Tags

Flag

Expression

Mammalian

Available Since

Aug. 15, 2024

Availability

Academic Institutions and Nonprofits only

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mNeongreen-PXN

Plasmid

#129604

Purpose

In vivo visualization of focal adhesions (can be used for colocalization studies)

Depositor

Dorus Gadella

Article

Chertkova et al bioRxiv (2020) 160374; doi: https://doi.

Insert

Paxillin

Expression

Mammalian

Promoter

CMV

Available Since

Jan. 7, 2020

Availability

Academic Institutions and Nonprofits only

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pLV-Bsd-CMV>tTS/rtTA:T2A:{cymR}

Plasmid

#236251

Purpose

Blasticidin selected lentiviral vector with Tet-On regulatory proteins and the cumate repressor, for use with dual-inducible CymR-CuO system

Depositor

Genevieve Kendall

Article

Kent et al Sci Rep. 2024 Sep 5;14(1):20773. doi: 10

Inserts

tTS/rtTA
cymR

Use

Lentiviral

Expression

Mammalian

Mutation

rTS and rtTA_M2 linked by T2A

Promoter

CMV

Available Since

June 12, 2025

Availability

Academic Institutions and Nonprofits only

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pLKO-Tet-On-YAP1-shRNA1

Plasmid

#176846

Purpose

lentiviral vector for doxycycline-inducible human YAP1 knockdown

Depositor

Roland Friedel

Article

Junqueira Alves et al Nat Commun. 2021 Oct 14;12(1):6019. doi:

Insert

YAP1-Sh1 (YAP1 Human)

Use

Lentiviral

Available Since

Nov. 5, 2021

Availability

Academic Institutions and Nonprofits only

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pLVX-EF1a-EGFP-VAMP2-IRES-Puromycin

Plasmid

#134661

Purpose

Expresses EGFP-tagged synaptic vesicle protein

Depositor

David Andrews

Article

Schormann et al J Cell Biol. 2020 Mar 2;219(3). pii: 133

Insert

vesicle-associated membrane protein 2 (VAMP2 Human)

Use

Lentiviral

Tags

EGFP

Expression

Mammalian

Promoter

EF1a

Available Since

March 2, 2020

Availability

Academic Institutions and Nonprofits only

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pScalps_Puro_mTet2 catalytic domain HxD

Plasmid

#79611

Purpose

Expression of catalytically inactive mouse Tet2

Depositor

Silvia Monticelli

Article

Montagner et al Cell Rep. 2016 May 17;15(7):1566-79. doi

Insert

Tet2 (Tet2 Mouse)

Use

Lentiviral

Tags

Myc

Mutation

Mutant Tet2 with H1302Y, D1304A substitutions in …

Available Since

July 21, 2016

Availability

Academic Institutions and Nonprofits only
pMK284 (Stag-3FLAG-Hygro)

Plasmid

#72800

Purpose

C-terminal tagging with Stag-3FLAG using CRIPSR/Cas9

Depositor

Masato Kanemaki

Article

Natsume et al Cell Rep. 2016 Apr 5;15(1):210-8. doi: 1

Insert

Stag-3FLAG-Hygro

Use

CRISPR

Tags

Stag-3FLAG

Expression

Mammalian

Available Since

March 24, 2016

Availability

Academic Institutions and Nonprofits only

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pJFRC206-10XUAS-FRT>STOP>FRT-myr::smGFP-V5

Plasmid

#63168

Purpose

GAL4-responsive UAS “flp-On” spaghetti monster GFP-V5 reporter

Depositor

Gerald Rubin

Article

Nern et al Proc Natl Acad Sci U S A. 2015 May 11. p

Insert

"spaghetti monster GFP-V5"

Tags

N-myristoylation

Expression

Insect

Promoter

GAL4-responsive UAS hsp70

Available Since

June 25, 2015

Availability

Academic Institutions and Nonprofits only

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pXPE

Plasmid

#15284

Depositor

Ron Vale

Article

Navone et al J Cell Biol. 1992 Jun . 117(6):1263-75.

Insert

ubiquitous kinesin heavy chain (KIF5B Human)

Expression

Bacterial

Mutation

The insert is missing the 1st 180 bp of 5' u…

Available Since

Oct. 12, 2007

Availability

Academic Institutions and Nonprofits only
Red Glifon Nega

Plasmid

#163117

Purpose

Red fluorescent protein-based glucose negative control sensor (does not respond to glucose) for mammalian cells

Depositor

Takashi Tsuboi

Article

Mita et al Cell Chem Biol. 2021 Jun 29. pii: S2451-

Insert

Red Glifon Nega

Expression

Mammalian

Promoter

CMV

Available Since

July 9, 2021

Availability

Academic Institutions and Nonprofits only

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