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SJZ10-LEU2

Plasmid

#228262

Purpose

Template plasmid for yeast gene knockout, uses auxotrophic marker LEU2 (Kluyveromyces lactis)

Depositor

Zhiping Xie

Article

Feng et al Microbiol Spectr. 2024 Oct 10:e0132024.

Insert

LEU2

Expression

Yeast

Promoter

LEU2 K.l.

Available Since

Nov. 21, 2024

Availability

Academic Institutions and Nonprofits only

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DOX: FSHB-t2a-GFP

Plasmid

#205595

Purpose

an overexpression vector of the killifish FSHB under doxycycline control

Depositor

Itamar Harel

Article

Moses et al eLife. 2023 Oct 24;12:e85960. doi: 10.75

Insert

FSHB

Tags

GFP

Promoter

Ef1a

Available Since

March 11, 2024

Availability

Academic Institutions and Nonprofits only

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pJZC530

Plasmid

#135434

Purpose

Yeast integrating plasmid for pTETO1-Venus reporter gene

Depositor

Jesse Zalatan

Article

Cunningham-Bryant et al Chembiochem. 2019 Jun 14;20(12):1519-152

Insert

pTETO1_Venus

Expression

Yeast

Promoter

pTETO1

Available Since

Sept. 17, 2020

Availability

Academic Institutions and Nonprofits only

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pTCO2

Plasmid

#149428

Purpose

cloning vector with regulatory regions of the murine Tdg gene

Depositor

Primo Schaer

Article

Schwarz et al F1000Res. 2020 Sep 9;9:1112. doi: 10.126

Type

Empty backbone

Use

Cre/Lox

Expression

Mammalian

Promoter

murine Tdg promoter

Available Since

Sept. 10, 2020

Availability

Academic Institutions and Nonprofits only

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pJS032

Plasmid

#135431

Purpose

Yeast integrating plasmid to express PYL1-VP64

Depositor

Jesse Zalatan

Article

Cunningham-Bryant et al Chembiochem. 2019 Jun 14;20(12):1519-152

Insert

PYL1-VP64

Expression

Yeast

Promoter

pAdh

Available Since

July 21, 2020

Availability

Academic Institutions and Nonprofits only

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pJS033

Plasmid

#135432

Purpose

Yeast integrating plasmid to express MCP-ABI

Depositor

Jesse Zalatan

Article

Cunningham-Bryant et al Chembiochem. 2019 Jun 14;20(12):1519-152

Insert

MCP-ABI

Expression

Yeast

Promoter

pTEF1

Available Since

July 21, 2020

Availability

Academic Institutions and Nonprofits only

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NK651

Plasmid

#109107

Purpose

Reporter gene expression in S. rosetta

Depositor

Nicole King

Article

Booth et al Mol Biol Cell. 2018 Dec 1;29(25):3026-30

Insert

PTSG_07215 deltaCC

Use

Choanoflagellate expression

Mutation

Deletes C-terminal coiled-coil from PTSG_07215

Available Since

July 10, 2018

Availability

Academic Institutions and Nonprofits only

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NK633

Plasmid

#109097

Purpose

Reporter gene expression in S. rosetta

Depositor

Nicole King

Article

Booth et al Mol Biol Cell. 2018 Dec 1;29(25):3026-30

Insert

mTFP1_3'UTR Act

Use

Choanoflagellate expression

Available Since

July 10, 2018

Availability

Academic Institutions and Nonprofits only

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NK617

Plasmid

#109095

Purpose

Reporter gene expression in S. rosetta

Depositor

Nicole King

Article

Booth et al Mol Biol Cell. 2018 Dec 1;29(25):3026-30

Insert

S. cerevisiae CoxIV target sequence

Use

Choanoflagellate expression

Available Since

May 15, 2018

Availability

Academic Institutions and Nonprofits only

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NK624

Plasmid

#109094

Purpose

Reporter gene expression in S. rosetta

Depositor

Nicole King

Article

Booth et al Mol Biol Cell. 2018 Dec 1;29(25):3026-30

Insert

HDEL peptide

Use

Choanoflagellate expression

Available Since

May 15, 2018

Availability

Academic Institutions and Nonprofits only

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pMBP-MS2

Plasmid

#65104

Purpose

can be used to isolate RNA binding complexes

Depositor

Josep Vilardell

Article

Macias et al Mol Cell. 2008 Jun 20;30(6):732-42. doi:

Insert

MS2

Tags

MBP

Expression

Bacterial

Mutation

double mutation (V75Q and A81G) that prevents oli…

Promoter

tac

Available Since

May 13, 2015

Availability

Academic Institutions and Nonprofits only

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UnReg-H2B-YFP-DD

Plasmid

#96892

Purpose

Reporter to monitor translation of unstructured mRNA

Depositor

Tobias Meyer

Article

Han et al Nat Methods. 2014 Jan;11(1):86-93. doi:

Insert

UnReg-H2B-Venus-DD

Use

Lentiviral

Expression

Mammalian

Available Since

Aug. 9, 2017

Availability

Academic Institutions and Nonprofits only

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IRES-H2B-YFP-DD

Plasmid

#96893

Purpose

Reporter to monitor translation of EMCV IRES mRNA

Depositor

Tobias Meyer

Article

Han et al Nat Methods. 2014 Jan;11(1):86-93. doi:

Insert

IRES-H2B-Venus-DD

Use

Lentiviral

Expression

Mammalian

Available Since

Sept. 6, 2017

Availability

Academic Institutions and Nonprofits only

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GFP_CD47_LU

Plasmid

#65474

Purpose

GFP tagged CD47 with its own long 3'UTR

Depositor

Christine Mayr

Article

Berkovits et al Nature. 2015 Apr 20. doi: 10.1038/nature

Inserts

Signal peptide of CD47
GFP
CD47 coding region continued and long 3’UTR (with proximal polyadenylation signal changed from AAUAAA to ACUCAA).

Expression

Mammalian

Available Since

June 25, 2015

Availability

Academic Institutions and Nonprofits only

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pCLX-pTF-R1-DEST-R2-EBR65

Plasmid

#45952

Depositor

Patrick Salmon

Article

Giry-Laterriere et al Hum Gene Ther. 2011 Oct;22(10):1255-67.

Type

Empty backbone

Use

Lentiviral

Available Since

Aug. 2, 2013

Availability

Academic Institutions and Nonprofits only

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AAV-TRE-PFE3-P2A-GFP

Plasmid

#236180

Purpose

AAV version of Tet inducible PFE3 for ablating excitatory synapses.

Depositor

Don Arnold

Article

Bareghamyan et al eLife. 2025 Apr 29;13:RP103757. doi: 10.

Insert

PFE3-P2A-GFP

Use

AAV

Available Since

July 2, 2025

Availability

Academic Institutions and Nonprofits only

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AAV-CAG-PFE3-P2A-GFP

Plasmid

#236178

Purpose

AAV version of constitutive PFE3 for ablating excitatory synapses.

Depositor

Don Arnold

Article

Bareghamyan et al eLife. 2025 Apr 29;13:RP103757. doi: 10.

Insert

PFE3-P2A-GFP

Use

AAV

Available Since

July 1, 2025

Availability

Academic Institutions and Nonprofits only

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3n1 (572)

Plasmid

#70727

Purpose

BetI/PhlF/SrpR BCD7 3-node v41-1

Depositor

Richard Murray

Article

Niederholtmeyer et al Elife. 2015 Oct 2;4. doi: 10.7554/eLife.

Inserts

srpR-ssrA
betI-ssrA
phlF-ssrA

Available Since

May 20, 2016

Availability

Academic Institutions and Nonprofits only

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pLV.PGK.GFP.miR9T

Plasmid

#115973

Purpose

miR-9 regulated GFP expression

Depositor

Johan Jakobsson

Article

Akerblom et al Nat Commun. 2013;4:1770. doi: 10.1038/nc

Insert

GFPmiR9T

Use

Lentiviral

Tags

GFP

Promoter

PGK

Available Since

Feb. 27, 2019

Availability

Academic Institutions and Nonprofits only

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pMIG-PP2A-Abeta

Plasmid

#15248

Depositor

William Hahn

Article

Sablina et al Cell. 2007 Jun 1. 129(5):969-82.

Insert

PP2A regulatory subunit A, beta isoform (PPP2R1B Human)

Use

Retroviral

Expression

Mammalian

Available Since

July 12, 2007

Availability

Academic Institutions and Nonprofits only