Sequence Type

BLAST Database

BLAST Algorithm

Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

We narrowed to 26,655 results for: Vit;

Showing: 10521 - 10560 of 26655 results

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pHAGE2-TetO-SOX17E57C

Plasmid

#216178

Purpose

Generate lentiviruses encoding for human SOX17E57C (HMG box numbering); for use in pluripotency reprogramming

Depositor

Ralf Jauch

Article

Ho et al Stem Cell Reports. 2025 Mar 11;20(3):102

Insert

SOX17 (SOX17 Human)

Use

Lentiviral

Mutation

Glu113Glu (silent mutation); Glu120Glu (silent mu…

Available Since

July 2, 2025

Availability

Academic Institutions and Nonprofits only

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pHAGE2-TetO-SOX17E57D

Plasmid

#216179

Purpose

Generate lentiviruses encoding for human SOX17E57D (HMG box numbering); for use in pluripotency reprogramming

Depositor

Ralf Jauch

Article

Ho et al Stem Cell Reports. 2025 Mar 11;20(3):102

Insert

SOX17 (SOX17 Human)

Use

Lentiviral

Mutation

Glu113Glu (silent mutation); Glu120Glu (silent mu…

Available Since

July 2, 2025

Availability

Academic Institutions and Nonprofits only

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pHAGE2-TetO-SOX17E57G

Plasmid

#216181

Purpose

Generate lentiviruses encoding for human SOX17E57G (HMG box numbering); for use in pluripotency reprogramming

Depositor

Ralf Jauch

Article

Ho et al Stem Cell Reports. 2025 Mar 11;20(3):102

Insert

SOX17 (SOX17 Human)

Use

Lentiviral

Mutation

Glu113Glu (silent mutation); Glu120Glu (silent mu…

Available Since

July 2, 2025

Availability

Academic Institutions and Nonprofits only

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pHAGE2-TetO-SOX17E57H

Plasmid

#216182

Purpose

Generate lentiviruses encoding for human SOX17E57H (HMG box numbering); for use in pluripotency reprogramming

Depositor

Ralf Jauch

Article

Ho et al Stem Cell Reports. 2025 Mar 11;20(3):102

Insert

SOX17 (SOX17 Human)

Use

Lentiviral

Mutation

Glu113Glu (silent mutation); Glu120Glu (silent mu…

Available Since

July 2, 2025

Availability

Academic Institutions and Nonprofits only

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pHAGE2-TetO-SOX17E57I

Plasmid

#216183

Purpose

Generate lentiviruses encoding for human SOX17E57I (HMG box numbering); for use in pluripotency reprogramming

Depositor

Ralf Jauch

Article

Ho et al Stem Cell Reports. 2025 Mar 11;20(3):102

Insert

SOX17 (SOX17 Human)

Use

Lentiviral

Mutation

Glu113Glu (silent mutation); Glu120Glu (silent mu…

Available Since

July 2, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pHAGE2-TetO-SOX17E57K

Plasmid

#216184

Purpose

Generate lentiviruses encoding for human SOX17E57K (HMG box numbering); for use in pluripotency reprogramming

Depositor

Ralf Jauch

Article

Ho et al Stem Cell Reports. 2025 Mar 11;20(3):102

Insert

SOX17 (SOX17 Human)

Use

Lentiviral

Mutation

Glu113Glu (silent mutation); Glu120Glu (silent mu…

Available Since

July 2, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pHAGE2-TetO-SOX17E57L

Plasmid

#216185

Purpose

Generate lentiviruses encoding for human SOX17E57L (HMG box numbering); for use in pluripotency reprogramming

Depositor

Ralf Jauch

Article

Ho et al Stem Cell Reports. 2025 Mar 11;20(3):102

Insert

SOX17 (SOX17 Human)

Use

Lentiviral

Mutation

Glu113Glu (silent mutation); Glu120Glu (silent mu…

Available Since

July 2, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pHAGE2-TetO-SOX17E57M

Plasmid

#216186

Purpose

Generate lentiviruses encoding for human SOX17E57M (HMG box numbering); for use in pluripotency reprogramming

Depositor

Ralf Jauch

Article

Ho et al Stem Cell Reports. 2025 Mar 11;20(3):102

Insert

SOX17 (SOX17 Human)

Use

Lentiviral

Mutation

Glu113Glu (silent mutation); Glu120Glu (silent mu…

Available Since

July 2, 2025

Availability

Academic Institutions and Nonprofits only

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pHAGE2-TetO-SOX17E57N

Plasmid

#216187

Purpose

Generate lentiviruses encoding for human SOX17E57N (HMG box numbering); for use in pluripotency reprogramming

Depositor

Ralf Jauch

Article

Ho et al Stem Cell Reports. 2025 Mar 11;20(3):102

Insert

SOX17 (SOX17 Human)

Use

Lentiviral

Mutation

Glu113Glu (silent mutation); Glu120Glu (silent mu…

Available Since

July 2, 2025

Availability

Academic Institutions and Nonprofits only

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pKLV2-U6gRNA5(hRNF31_2b)-PGKpuro2ABFP-W

Plasmid

#208415

Purpose

Lentiviral gRNA plasmid targeting human RNF31 gene, co-expression of BFP tag

Depositor

Sok Ching Cheong

Article

Chai et al Cancer Res Commun. 2024 Nov 1;4(11):2919

Insert

RNF31 (RNF31 Human)

Use

CRISPR and Lentiviral

Expression

Mammalian

Mutation

N/A

Promoter

human U6

Available Since

July 1, 2025

Availability

Academic Institutions and Nonprofits only

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pKLV2-U6gRNA5(hRNF31_1k)-PGKpuro2ABFP-W

Plasmid

#208414

Purpose

Lentiviral gRNA plasmid targeting human RNF31 gene, co-expression of BFP tag

Depositor

Sok Ching Cheong

Article

Chai et al Cancer Res Commun. 2024 Nov 1;4(11):2919

Insert

RNF31 (RNF31 Human)

Use

CRISPR and Lentiviral

Expression

Mammalian

Mutation

N/A

Promoter

human U6

Available Since

July 1, 2025

Availability

Academic Institutions and Nonprofits only

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proUBI10::VIH2-FL::FLAG-H741A

Plasmid

#238335

Purpose

proUBI10::VIH2-FL::FLAG-H741A ubiquitous expression in Arabidopsis plants

Depositor

Michael Hothorn

Article

Raia et al Nat Commun. 2025 Feb 19;16(1):1753. doi:

Insert

VIP1 (VIP1 Mustard Weed)

Tags

3x Flag-tag

Expression

Plant

Mutation

AtVIH2-H741A

Available Since

June 27, 2025

Availability

Academic Institutions and Nonprofits only

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pBB3-ScVip1-536-1107-K558A-K605A-K732A-K817A

Plasmid

#238325

Purpose

ScVip1-536-1107-K558A-K605A-K732A-K817A protein expression in insect cells

Depositor

Michael Hothorn

Article

Raia et al Nat Commun. 2025 Feb 19;16(1):1753. doi:

Insert

VIP1 (VIP1 Budding Yeast)

Tags

His10-Tag, Twin-Strep-tag, TEV

Expression

Insect

Mutation

ScVip1-536-1107-K558A-K605A-K732A-K817A

Available Since

June 12, 2025

Availability

Academic Institutions and Nonprofits only

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proUBI10::VIH2-FL::FLAG-C696A

Plasmid

#238336

Purpose

proUBI10::VIH2-FL::FLAG-C696A ubiquitous expression in Arabidopsis plants

Depositor

Michael Hothorn

Article

Raia et al Nat Commun. 2025 Feb 19;16(1):1753. doi:

Insert

VIP1 (VIP1 Mustard Weed)

Tags

3x Flag-tag

Expression

Plant

Mutation

AtVIH2-C696A

Available Since

June 12, 2025

Availability

Academic Institutions and Nonprofits only

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proUBI10::VIH2-FL::FLAG-K219A-D292A

Plasmid

#238331

Purpose

proUBI10::VIH2-FL::FLAG-K219A-D292A ubiquitous expression in Arabidopsis plants

Depositor

Michael Hothorn

Article

Raia et al Nat Commun. 2025 Feb 19;16(1):1753. doi:

Insert

VIP1 (VIP1 Mustard Weed)

Tags

3x Flag-tag

Expression

Plant

Mutation

AtVIH2-K219A-D292A

Available Since

June 12, 2025

Availability

Academic Institutions and Nonprofits only

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proUBI10::VIH2-FL::FLAG-R372A-H373A

Plasmid

#238332

Purpose

proUBI10::VIH2-FL::FLAG-R372A-H373A ubiquitous expression in Arabidopsis plants

Depositor

Michael Hothorn

Article

Raia et al Nat Commun. 2025 Feb 19;16(1):1753. doi:

Insert

VIP1 (VIP1 Mustard Weed)

Tags

3x Flag-tag

Expression

Plant

Mutation

AtVIH2-AtVIH2-R372A-H373A

Available Since

June 12, 2025

Availability

Academic Institutions and Nonprofits only

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proUBI10::VIH2-FL::FLAG-Wild type

Plasmid

#238330

Purpose

proUBI10::VIH2-FL::FLAG-Wild type ubiquitous expression in Arabidopsis plants

Depositor

Michael Hothorn

Article

Raia et al Nat Commun. 2025 Feb 19;16(1):1753. doi:

Insert

VIP1 (VIP1 Mustard Weed)

Tags

3x Flag-tag

Expression

Plant

Mutation

AtVIH2-Wild type

Available Since

June 12, 2025

Availability

Academic Institutions and Nonprofits only

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pcDNA3-Lyso-ExRai-CKAR2

Plasmid

#236100

Purpose

Enhanced excitation-ratiometric biosensor for monitoring Protein Kinase C activity in living cells, targeted to lysosome surface.

Depositor

Jin Zhang

Article

Su et al Nat Chem Biol. 2024 Oct 11. doi: 10.1038

Insert

LAMP1-ExRai-CKAR2 (LAMP1 Synthetic, Human)

Tags

Full-length LAMP1

Expression

Mammalian

Promoter

CMV

Available Since

June 3, 2025

Availability

Academic Institutions and Nonprofits only

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pLIB-Strep(II)(C)-CTR9

Plasmid

#231725

Purpose

Protein expression in insect cells

Depositor

Jesper Svejstrup

Article

Lopez Martinez et al Mol Cell. 2025 May 15;85(10):1952-1967.e

Insert

CTR9 (CTR9 Human)

Tags

3C-Strep(II)

Expression

Insect

Available Since

May 2, 2025

Availability

Academic Institutions and Nonprofits only

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pLIB-FLAG(N)-CDC73

Plasmid

#231726

Purpose

Protein expression in insect cells

Depositor

Jesper Svejstrup

Article

Lopez Martinez et al Mol Cell. 2025 May 15;85(10):1952-1967.e

Insert

CDC73 (CDC73 Human)

Tags

Flag-3C

Expression

Insect

Available Since

May 2, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pLIB-FLAG(N)-CDK12deltaCTD(1-1082)

Plasmid

#231735

Purpose

Protein expression in insect cells

Depositor

Jesper Svejstrup

Article

Lopez Martinez et al Mol Cell. 2025 May 15;85(10):1952-1967.e

Insert

CDK12 (CDK12 Human)

Tags

Flag-3C

Expression

Insect

Mutation

CDK12(1-1082)

Available Since

May 2, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pLIB-FLAG(N)-CDK12

Plasmid

#231716

Purpose

Protein expression in insect cells

Depositor

Jesper Svejstrup

Article

Lopez Martinez et al Mol Cell. 2025 May 15;85(10):1952-1967.e

Insert

CDK12 (CDK12 Human)

Tags

Flag-3C

Expression

Insect

Available Since

May 2, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pLIB-FLAG(N)-CDK13

Plasmid

#231717

Purpose

Protein expression in insect cells

Depositor

Jesper Svejstrup

Article

Lopez Martinez et al Mol Cell. 2025 May 15;85(10):1952-1967.e

Insert

CDK13 (CDK13 Human)

Tags

Flag-3C

Expression

Insect

Available Since

May 2, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pLIB-FLAG(N)-CDK9

Plasmid

#231719

Purpose

Protein expression in insect cells

Depositor

Jesper Svejstrup

Article

Lopez Martinez et al Mol Cell. 2025 May 15;85(10):1952-1967.e

Insert

CDK9 (CDK9 Human)

Tags

Flag-3C

Expression

Insect

Available Since

May 2, 2025

Availability

Academic Institutions and Nonprofits only

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pVEA3-KNC

Plasmid

#234801

Purpose

Mammalian expression of bovine arrestin-3 (K11A, K12A, L49A, D51A, R52A, L69A, Y239A, D241A, C252A, P253A, D260A, Q262A)

Depositor

Vsevolod Gurevich

Article

Breitman et al J Biol Chem. 2012 Jun 1;287(23):19653-64

Insert

Arrestin-3 (ARR3 Bovine)

Tags

Venus

Expression

Mammalian

Mutation

K11A, K12A, L49A, D51A, R52A, L69A, Y239A, D241A,…

Available Since

April 23, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pET-HLA-A0201-ST3

Plasmid

#223220

Purpose

Expression of human HLA A2 heavy chain fused to Spytag003 for expression in bacteria

Depositor

Omer Dushek

Article

Patel et al EMBO J. 2024 Jan;43(1):132-150. doi: 10.

Insert

HLA--A2 heavy chain (HLA-A Human)

Tags

Spytag

Expression

Bacterial

Promoter

T7

Available Since

March 4, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pFastBacI-KDAC8

Plasmid

#224343

Purpose

Expresses human KDAC8 (HDAC8) in insect cells

Depositor

Terry Watt

Article

Toro et al Protein Expr Purif. 2018 Jan;141:1-6. do

Insert

KDAC8 (HDAC8 Human)

Tags

TEV-cleavable His6

Expression

Insect

Promoter

Polyhedrin

Available Since

Feb. 26, 2025

Availability

Industry, Academic Institutions, and Nonprofits

Enlarge
pEIF3D-S528D-S529D-sgEIF3D

Plasmid

#222127

Purpose

EIF3D variant overexpression vector with sgRNA targeting eIF3D

Depositor

Jonathan Weissman

Article

She et al Nucleic Acids Res. 2023 Jul 7;51(12):635

Insert

eIF3D (EIF3D Human)

Use

Lentiviral

Tags

BFP-P2A

Mutation

S528D, S529D

Available Since

Feb. 3, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pEIF3D-NtermDel-CtermDel-sgEIF3D

Plasmid

#222131

Purpose

EIF3D variant overexpression vector with sgRNA targeting eIF3D

Depositor

Jonathan Weissman

Article

She et al Nucleic Acids Res. 2023 Jul 7;51(12):635

Insert

eIF3D (EIF3D Human)

Use

Lentiviral

Tags

BFP-P2A

Mutation

161 + 527 truncation

Available Since

Feb. 3, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pCMV-HA Set1

Plasmid

#192219

Purpose

It expresses HA-fused mouse Set1 isoform protein in mammalian cells

Depositor

Hisakazu Ogita

Article

Molla et al Commun Biol. 2022 Oct 7;5(1):1071. doi:

Insert

Set isoform 1 (Set Mouse)

Tags

HA

Expression

Mammalian

Promoter

CMV

Available Since

Dec. 17, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pCMV-HA Set2

Plasmid

#192220

Purpose

It expresses HA-fused mouse Set2 isoform protein in mammalian cells

Depositor

Hisakazu Ogita

Article

Molla et al Commun Biol. 2022 Oct 7;5(1):1071. doi:

Insert

Set isoform 2 (Set Mouse)

Tags

HA

Expression

Mammalian

Promoter

CMV

Available Since

Dec. 17, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pLentiCRISPRv2_sgALDH7A1

Plasmid

#218273

Purpose

CRISPR KO plasmid for ALDH7A1 in human cell lines

Depositor

Seth Parker

Article

Johal et al Cell Rep. 2024 Dec 24;43(12):115069. doi

Insert

Inserted sgRNA targetting ALDH7A1 (ALDH7A1 Human)

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

U6

Available Since

Dec. 11, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pINQ-NbH4

Plasmid

#198690

Purpose

Periplasmic expression of anti-SARS-Cov-2 Nucleocapsid nanobody H4 (AviTag) in BL21(DE3)

Depositor

Gualberto González Sapienza

Article

Segovia-de Los Santos et al Front Immunol. 2023 Jul 11;14:1220477. d

Insert

anti-SARS-CoV-2 Nucleocapsid nanobody H4

Tags

6xHis tag, Avitag, OmpA signal peptide, and Ribos…

Expression

Bacterial

Promoter

T7 promoter

Available Since

Dec. 11, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pJExpress401-KDAC4 CD H976Y

Plasmid

#224250

Purpose

Expresses human KDAC4 (HDAC4) catalytic domain H976Y in E. coli

Depositor

Terry Watt

Article

Toro et al Biochemistry. 2017 Aug 29;56(34):4549-45

Insert

KDAC4 (HDAC4 Human)

Tags

TEV-cleavable His6

Expression

Bacterial

Mutation

Only includes residues 648-1083 (catalytic domain…

Promoter

T5

Available Since

Oct. 22, 2024

Availability

Industry, Academic Institutions, and Nonprofits

Enlarge
pFastBacI-KDAC6 H216A

Plasmid

#224351

Purpose

Expresses human KDAC6 (HDAC6) H216A in insect cells

Depositor

Terry Watt

Article

Toro et al Biochemistry. 2023 May 2;62(9):1464-1483

Insert

KDAC6 (HDAC6 Human)

Tags

TEV-cleavable His6

Expression

Insect

Mutation

Histidine 216 mutated to alanine

Promoter

Polyhedrin

Available Since

Oct. 22, 2024

Availability

Industry, Academic Institutions, and Nonprofits

Enlarge
pFastBacI-KDAC6 H611A

Plasmid

#224352

Purpose

Expresses human KDAC6 (HDAC6) H611A in insect cells

Depositor

Terry Watt

Article

Toro et al Biochemistry. 2023 May 2;62(9):1464-1483

Insert

KDAC6 (HDAC6 Human)

Tags

TEV-cleavable His6

Expression

Insect

Mutation

Histidine 611 mutated to alanine

Promoter

Polyhedrin

Available Since

Oct. 22, 2024

Availability

Industry, Academic Institutions, and Nonprofits

Enlarge
pCDNA3-Regnase-1 D141N

Plasmid

#222654

Purpose

Mammalian expression vector to express mouse Regnase-1 D141N mutant

Depositor

Silvia Monticelli

Article

Bataclan et al Life Sci Alliance. 2024 Jun 3;7(8):e2024

Insert

Zc3h12a (Zc3h12a Mouse)

Expression

Mammalian

Mutation

mutated aspartic acid 141 to asparagine to abroga…

Promoter

CMV

Available Since

Oct. 8, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pUC57 mini-ZsGreen-P2A-FLAG-HA-Regnase-1 WT

Plasmid

#222649

Purpose

For in vitro transcription of mouse Zc3h12a tagged with FLAG-HA at N-term

Depositor

Silvia Monticelli

Article

Bataclan et al Life Sci Alliance. 2024 Jun 3;7(8):e2024

Insert

Zc3h12a (Zc3h12a Mouse)

Use

In vitro transcription

Tags

FLAG and HA

Promoter

T7

Available Since

Oct. 8, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pEIF3D-S528N-S529N-sgEIF3D

Plasmid

#222129

Purpose

EIF3D variant overexpression vector with sgRNA targeting eIF3D

Depositor

Jonathan Weissman

Article

She et al Nucleic Acids Res. 2023 Jul 7;51(12):635

Insert

eIF3D (EIF3D Human)

Use

Lentiviral

Tags

BFP-P2A

Mutation

S528N, S529N

Available Since

Oct. 4, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pGEMHE:mTREK-1(tandem S131C-S131C)

Plasmid

#224760

Purpose

In vitro transcription for Oocyte expression of mTREK-1(tandem S131C-S131C)

Depositor

Dan Minor

Article

Deal et al Cell Chem Biol. 2024 Jul 18;31(7):1305-1

Insert

Tandem TREK-1 (S131C-S131C) (Kcnk2 Mouse)

Use

In vitro transcription for oocyte expression

Mutation

S131C / S562C

Promoter

T7

Available Since

Sept. 13, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge

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