Skip to main content
Addgene
Open Menu

Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

Information

In Category

Filter By

We narrowed to 14,352 results for: Cas9

Clear keywords
Showing: 5261 - 5280 of 14352 results

  1. Polymerase variant click editor (CE1) - pCMV-T7-PCV2-nCas9-Phi29 (JO582)

    Plasmid
    #208954
    Purpose
    A variant CE1 construct with Phi29 DNA polymerase (-exo), expressed from CMV or T7 promoters.
    Depositor
    Benjamin Kleinstiver
    Publication
    Ferreira da Silva et al Nat Biotechnol. 2024 Jul 22. doi: 10.103
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    PCV2-XTEN-nSpCas9-BPNLS-Phi29(-exo)-BPNLS
    Use
    CRISPR; In vitro transcription; t7 promoter
    Tags
    BPNLS
    Expression
    Mammalian
    Mutation
    nSpCas9(H840A), Phi29(-exo;D169A)
    Promoter
    CMV and T7
    Available since
    Nov. 27, 2023
    Availability
    Academic Institutions and Nonprofits only
    208954-plasmid-map-sequence-id-415743

    Enlarge

  2. pCMV-SaPE2-P2A-BSD

    Plasmid
    #174818
    Purpose
    Mammalian expression of SaCas9 prime editor 2 with P2A-BSD marker
    Depositor
    David Liu
    Publication
    Chen et al Cell. 2021 Oct 28;184(22):5635-5652.e29.
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    SaPE2-P2A-BSD
    Tags
    SV40 bpNLS
    Expression
    Mammalian
    Mutation
    Detailed in manuscript
    Promoter
    CMV
    Available since
    Oct. 14, 2021
    Availability
    Academic Institutions and Nonprofits only
    174818-plasmid-map-sequence-id-343609

    Enlarge

  4. pLV_hPGK-2xMeCP2NLS-dCas9-HA-2xSV40NLS-KOX1-NpNLS-P2A/T2A-mTagBFP2-2xMycNLS-WPRE

    Plasmid
    #254799
    Purpose
    Constituitve expression of dCas9-KOX1 with optimized nuclear localization in iPSC-derived neurons via lenti delivery
    Depositor
    Ophir Shalem
    Publication
    Cajka et al bioRxiv [Preprint]. 2024 Oct 19:2024.10.
    Insert
    2xMeCP2NLS-dCas9-HA-2xSV40NLS-KOX1-NpNLS-P2A/T2A-mTagBFP2-2xMycNLS
    Use
    CRISPR and Lentiviral
    Expression
    Mammalian
    Mutation
    catalytically dead spCas9
    Promoter
    hPGK
    Available since
    Aug. 31, 2026
    Availability
    Academic Institutions and Nonprofits only
    254799-plasmid-map-sequence-id-520123

    Enlarge

  7. px330_PTEN exon 5 gRNA

    Plasmid
    #68348
    Purpose
    px330 with gRNA towards PTEN exon 5. Cas9 is expressed from a CAG promoter.
    Depositor
    Jannik Elverløv-Jakobsen
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    PTEN exon 5 gRNA
    Expression
    Mammalian
    Promoter
    U6
    Available since
    Oct. 5, 2015
    Availability
    Academic Institutions and Nonprofits only
    68348-plasmid-map-sequence-id-119620

    Enlarge

  10. pMTL3

    Plasmid
    #127966
    Purpose
    constitutive expression of KRAB-dCas9-BFP-KRAB from the AAVS1 locus
    Depositor
    Martin Kampmann
    Publication
    Tian et al Neuron. 2019 Aug 5. pii: S0896-6273(19)3
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    AAVS1-CAG-KRAB-dCas9-BFP-KRAB
    Use
    TALEN
    Expression
    Mammalian
    Promoter
    CAG
    Available since
    Nov. 23, 2019
    Availability
    Academic Institutions and Nonprofits only
    127966-plasmid-map-sequence-id-249160

    Enlarge

  12. pMTL5

    Plasmid
    #127967
    Purpose
    DHFR-degron controlled expression of KRAB-dCas9-BFP-KRAB from the AAVS1 locus
    Depositor
    Martin Kampmann
    Publication
    Tian et al Neuron. 2019 Aug 5. pii: S0896-6273(19)3
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    AAVS1-CAG-DHFR-KRAB-dCas9-BFP-KRAB
    Use
    TALEN
    Expression
    Mammalian
    Promoter
    CAG
    Available since
    Nov. 4, 2019
    Availability
    Academic Institutions and Nonprofits only
    127967-plasmid-map-sequence-id-249184

    Enlarge

  16. pEvolvR-mCherry-Slug-nCas9(D10A)-PolI5MΔ

    Plasmid
    #249078
    Purpose
    Expresses nucleus-localized Slug-nCas9 (D10A) fused to PolI5MΔ and mCherry using a CMV promoter. Includes a GFP cassette flanked by BsmbI cut sites to knock in and coexpress user-defined gRNAs.
    Depositor
    John Dueber, David Schaffer
    Publication
    Hurtado et al Nat Commun. 2025 Apr 19;16(1):3723. doi:
    Insert
    Slug-nCas9-PolI5MΔ
    Use
    CRISPR
    Tags
    mCherry
    Expression
    Mammalian
    Mutation
    deletion of PolI5M N-terminal flap endonuclease d…
    Promoter
    CMV
    Available since
    Jan. 7, 2026
    Availability
    Academic Institutions and Nonprofits only
    249078-plasmid-map-sequence-id-496311

    Enlarge

  18. pAAV-synmiR-L-mRuby-Pro-dCas9-EGFP-4×19

    Plasmid
    #218270
    Purpose
    Expresses dCas9-EGFP in mammalian cells regulated by a synthetic microRNA-based dosage compensation circuit
    Depositor
    Michael Elowitz
    Insert
    synPolyA-synmiRL-mRuby-EF1a-CMVe_MeP-dCas9-EGFP-MREL_4×19
    Use
    AAV and Synthetic Biology
    Expression
    Mammalian
    Available since
    Dec. 12, 2025
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    218270-plasmid-map-sequence-id-494320

    Enlarge

  19. pGGB-OCS terminator-SunTag-HA-dCas9-NLS

    Plasmid
    #246798
    Purpose
    A GreenGate entry vector containing NLS-dCas9-HA-SunTag fused gene followed by a OCS terminator
    Depositor
    Danhua Jiang
    Insert
    NLS-dCas9-HA-SunTag-OCS terminator
    Use
    Greengate cloning entry vector
    Available since
    Dec. 2, 2025
    Availability
    Academic Institutions and Nonprofits only
    246798-plasmid-map-sequence-id-494307

    Enlarge

  20. AAV-U6-msCAMK2d-sgRNA-cTNT-SaCas9-HA-miR122 TS

    Plasmid
    #209783
    Purpose
    Expresses SaCas9 by the specific cTNT promoter and sgRNA targeted the mouse Camk2d gene by U6 promoter, incorporation of the miR122 target sequences (miR122TS) into the 3’ untranslated region (3’ UTR)
    Depositor
    Yuxuan Guo
    Publication
    Yang et al Circulation. 2024 May 28;149(22):1778-17
    Insert
    miR122 ts
    Use
    AAV
    Tags
    HA
    Promoter
    cTNT
    Available since
    May 29, 2024
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    209783-plasmid-map-sequence-id-415445

    Enlarge

Showing: 5261 - 5280 of 14352 results