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DinoIII-arrO-N

Plasmid

#128143

Purpose

Dinoflagellate backbone with rifampicin resistant gene optimized for Oxyrrhis marina

Depositor

Senjie Lin

Article

Sprecher et al Microorganisms. 2020 Jan 16;8(1). pii: m

Insert

Rifampin ADP-ribosylating transferase + potential promoter

Expression

Bacterial

Available Since

April 29, 2020

Availability

Academic Institutions and Nonprofits only

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pRS513

Plasmid

#17283

Depositor

Nancy Craig

Article

Skelding et al EMBO J. 2002 Jul 1. 21(13):3494-504.

Insert

TnsB (tnsB E. coli transposon Tn7)

Tags

Chitin-binding domain-intein

Expression

Bacterial

Available Since

April 3, 2008

Availability

Academic Institutions and Nonprofits only
pDSAP

Plasmid

#62293

Purpose

docking site transgenesis plasmid for GoldenGate Cloning and insect transgenesis, Puromycin resistance marker

Depositor

Eric Marois

Article

Volohonsky et al G3 (Bethesda). 2015 Apr 13. pii: g3.115.

Type

Empty backbone

Expression

Insect

Available Since

March 23, 2015

Availability

Academic Institutions and Nonprofits only

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pXCX.CMV.EGFP

Plasmid

#38053

Depositor

James Uney

Article

Glover et al Mol Ther. 2002 May;5(5 Pt 1):509-16.

Insert

EGFP

Use

Adenoviral

Expression

Mammalian

Promoter

CMV

Available Since

Oct. 15, 2012

Availability

Academic Institutions and Nonprofits only

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pDSAY

Plasmid

#62291

Purpose

docking site transgenesis plasmid for GoldenGate Cloning and insect transgenesis, YFP marker

Depositor

Eric Marois

Article

Volohonsky et al G3 (Bethesda). 2015 Apr 13. pii: g3.115.

Type

Empty backbone

Expression

Insect

Available Since

May 18, 2015

Availability

Academic Institutions and Nonprofits only

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p15A_J23108_SpydCas9_CmR

Plasmid

#119163

Purpose

Expresses S. pyogenes dCas9

Depositor

Vincent Noireaux

Article

Collias et al Synth Biol (Oxf). 2019 Jan 21;4(1):ysz00

Insert

dCas9 from S. pyogenes

Use

Crispr

Available Since

Dec. 18, 2018

Availability

Academic Institutions and Nonprofits only

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pXCX.CMV.EGFP.WPRE

Plasmid

#38055

Depositor

James Uney

Article

Glover et al Mol Ther. 2002 May;5(5 Pt 1):509-16.

Insert

EGFP

Use

Adenoviral

Expression

Mammalian

Promoter

CMV

Available Since

Oct. 15, 2012

Availability

Academic Institutions and Nonprofits only

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pH6-mNeon

Plasmid

#178460

Purpose

The mNeonGreen fluorescent protein with an N-terminal 6xHis-tag in pET28a.

Depositor

Siegfried Musser

Article

Sharma et al Commun Biol. 2022 Sep 19;5(1):988. doi:

Insert

mNeonGreen

Tags

6xHis

Expression

Bacterial

Promoter

T7

Available Since

July 5, 2023

Availability

Academic Institutions and Nonprofits only

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LV UAS-DsRed-Express2-PGK-Puro

Plasmid

#190801

Purpose

LV construct encoding UAS-DsRed-Express2 reporter gene.

Depositor

John Ngo

Article

Sloas et al Nat Biotechnol. 2023 Jan 16. doi: 10.103

Insert

UAS-T2A-DsRed-Express2

Use

Lentiviral and Synthetic Biology

Available Since

March 3, 2023

Availability

Academic Institutions and Nonprofits only

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pID2.02

Plasmid

#26955

Depositor

Geraldine Seydoux

Article

Gallo et al Dev Biol. 2008 Nov 1. 323(1):76-87.

Insert

unc-119 rescuing fragment/pie-1promoter-gateway destination cassette B- pie-1 3’

Expression

Worm

Available Since

March 18, 2011

Availability

Academic Institutions and Nonprofits only

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pSW104

Plasmid

#122295

Purpose

For run-off transcription of yeast RPL41A mRNA with and without an 82 nucleotide poly(A) tail.

Depositor

Sarah Walker

Article

Liu et al Methods. 2019 Mar 26. pii: S1046-2023(18

Insert

RPL41A

Use

In vitro transcription

Available Since

July 22, 2019

Availability

Academic Institutions and Nonprofits only

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pFastBac K-Ring1A

Plasmid

#1994

Depositor

Robert Kingston

Article

Lavigne et al Mol Cell 2004 Feb 13;13(3):415-25.

Insert

Ring1A (Ring1 Mouse)

Expression

Insect

Mutation

Kozak before start codon

Available Since

March 23, 2006

Availability

Academic Institutions and Nonprofits only
Cas9m3

Plasmid

#47315

Purpose

Cas9 D10A+D839A+H840A

Depositor

George Church

Article

Mali et al Nat Biotechnol. 2013 Aug 1. doi: 10.1038

Insert

Cas9m3

Use

CRISPR

Available Since

Sept. 4, 2013

Availability

Academic Institutions and Nonprofits only
pBS-M71-IRES-CreFNF

Plasmid

#15509

Depositor

Peter Mombaerts

Article

Li et al Nature. 2004 Mar 25. 428(6981):393-9.

Insert

M71 targeting vector with IRES CreFNF

Use

High copy number

Available Since

Aug. 24, 2007

Availability

Academic Institutions and Nonprofits only
pJBL6392

Plasmid

#193141

Purpose

Synthetic OFF-logic Clostridium beijerinckii pfl ZTP riboswitch with sfGFP reporter

Depositor

Julius Lucks

Article

Bushhouse et al Nucleic Acids Res. 2023 Mar 2:gkad110. d

Insert

Synthetic flipped logic Cbe riboswitch with sfGFP

Use

Synthetic Biology

Expression

Bacterial

Promoter

J23119

Available Since

March 8, 2023

Availability

Academic Institutions and Nonprofits only

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MiCy-C1

Plasmid

#54609

Purpose

Localization: C1 Cloning Vector, Excitation: 472, Emission: 495

Depositor

Michael Davidson, Atsushi Miyawaki

Article

Karasawa et al Biochem J. 2004 Jul 1. 381(Pt 1):307-12.

Type

Empty backbone

Tags

MiCy

Expression

Mammalian

Available Since

July 25, 2014

Availability

Academic Institutions and Nonprofits only
pBABE-FLAG-hG9a

Plasmid

#33023

Depositor

Martin Walsh

Article

Nishio et al Proc Natl Acad Sci U S A. 2004 Aug 3;101

Insert

hG9a

Use

Retroviral

Tags

Flag

Expression

Mammalian

Promoter

5'LTR

Available Since

April 2, 2012

Availability

Academic Institutions and Nonprofits only
sTag-BFP

Plasmid

#186903

Purpose

Cell-free expression of soluble protein tagBFP-sfCherry11-SpyTag

Depositor

Taekjip Ha, Allen Liu

Article

Moghimianavval et al Small. 2022 May 26:e2202104. doi: 10.100

Insert

SpyTag003-sfCherry11-tagBFP

Use

Synthetic Biology

Tags

tagBFP

Available Since

Aug. 2, 2022

Availability

Academic Institutions and Nonprofits only

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pJW1504

Plasmid

#62358

Purpose

RPS2 HA-TEV-AVI pop-in/pop-out plasmid

Depositor

Jonathan Weissman

Article

Jan et al Science. 2014 Nov 7;346(6210):1257521. d

Insert

RPS2 fragment

Use

Yeast targeting

Tags

HA-TEV-AVI

Available Since

May 13, 2015

Availability

Academic Institutions and Nonprofits only

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p{3P3-dsRed::FRT-RC,attB}

Plasmid

#182177

Purpose

fluorescent reporter gene with yeast recombination site within intron

Depositor

David Stern

Article

Stern et al G3 (Bethesda). 2022 Feb 10. pii: 6526388

Insert

DsRed

Expression

Insect

Available Since

March 28, 2022

Availability

Academic Institutions and Nonprofits only

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