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pSB1C3-J23110-B0034-mRFP1_Yellow

Plasmid

#160443

Purpose

BioBrick pSB1C3 plasmid that constitutively overexpresses mRFP1_Yellow chromoprotein in E. coli

Depositor

Anthony Forster

Article

Bao et al Anal Biochem. 2020 Sep 3:113936. doi: 10

Insert

promoter, RBS, mRFP1E_Yellow

Use

Synthetic Biology

Mutation

BioBrick sites removed

Available Since

Jan. 20, 2021

Availability

Academic Institutions and Nonprofits only

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Pramr-GFP

Plasmid

#194148

Purpose

Contains a RamR-regulated promoter expressing GFP

Depositor

Andrew Ellington

Article

d'Oelsnitz et al Nat Chem Biol. 2022 Jul 7. pii: 10.1038/

Insert

GFP

Expression

Bacterial

Promoter

Pramr

Available Since

Jan. 23, 2023

Availability

Academic Institutions and Nonprofits only

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pGT-Ah7

Plasmid

#122574

Purpose

MAGIC donor plasmid (Himar transposon with catP/sfGFP payload)

Depositor

Harris Wang

Article

Ronda et al Nat Methods. 2019 Feb;16(2):167-170. doi

Insert

catP/sfGFP

Use

Synthetic Biology

Expression

Bacterial

Available Since

May 7, 2019

Availability

Academic Institutions and Nonprofits only

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pUdO2c

Plasmid

#210231

Purpose

medium-copy plasmid for bacterial expression

Depositor

François-Xavier Campbell-Valois

Article

Manigat et al ACS Synth Biol. 2024 Jan 18. doi: 10.102

Type

Empty backbone

Expression

Bacterial

Available Since

Feb. 16, 2024

Availability

Academic Institutions and Nonprofits only

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pGT-Ah10

Plasmid

#122577

Purpose

MAGIC donor plasmid (Himar transposon with tetQ/sfGFP payload)

Depositor

Harris Wang

Article

Ronda et al Nat Methods. 2019 Feb;16(2):167-170. doi

Insert

tetQ/sfGFP

Use

Synthetic Biology

Expression

Bacterial

Available Since

May 6, 2019

Availability

Academic Institutions and Nonprofits only

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pWS481

Plasmid

#123029

Purpose

LexA UAS

Depositor

Tom Ellis

Article

Shaw et al Cell. 2019 Apr 18;177(3):782-796.e27. do

Insert

LexO (6x) [2a]

Expression

Yeast

Available Since

April 4, 2019

Availability

Academic Institutions and Nonprofits only

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pKM493

Plasmid

#140191

Purpose

ORBIT integrating plasmid for C-terminal tagging with cleavable EGFP.

Depositor

Kenan Murphy

Article

Murphy et al MBio. 2018 Dec 11;9(6). pii: mBio.01467-

Insert

TEV-Flag-Gly4-eGFP

Tags

Flag + eGFP

Expression

Bacterial

Promoter

PGroEL

Available Since

Jan. 2, 2024

Availability

Academic Institutions and Nonprofits only

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pBS-SKII-3XHA-fCLIP-NAT

Plasmid

#188921

Purpose

Integration cassette to create C-terminal fusions for fluorescent tagging

Depositor

Stephen Buratowski

Article

Baek et al G3 (Bethesda). 2022 Aug 9. pii: 6659097.

Insert

3XHA-fCLIP-NAT

Expression

Yeast

Available Since

Sept. 8, 2022

Availability

Academic Institutions and Nonprofits only

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pAB167

Plasmid

#101672

Purpose

Opto-T7RNAP*(563), equal expr.

Depositor

Mustafa Khammash

Article

Baumschlager et al ACS Synth Biol. 2017 Nov 17;6(11):2157-2

Insert

Opto-T7RNAP*(563), equal expr.

Use

Synthetic Biology

Expression

Bacterial

Available Since

Sept. 15, 2022

Availability

Academic Institutions and Nonprofits only

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pBS-SKII-3XHA-fSNAP-NAT

Plasmid

#188916

Purpose

Integration cassette to create C-terminal fusions for fluorescent tagging

Depositor

Stephen Buratowski

Article

Baek et al G3 (Bethesda). 2022 Aug 9. pii: 6659097.

Insert

3XHA-fSNAP-NAT

Expression

Yeast

Available Since

Sept. 8, 2022

Availability

Academic Institutions and Nonprofits only

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pBbA5c-RFP

Plasmid

#35281

Depositor

Jay Keasling

Article

Lee et al J Biol Eng. 2011 Sep 20;5:12.

Insert

RFP

Use

Synthetic Biology

Expression

Bacterial

Promoter

lacUV5

Available Since

Feb. 20, 2012

Availability

Academic Institutions and Nonprofits only

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pGT-Ah6

Plasmid

#122573

Purpose

MAGIC donor plasmid (Himar transposon with catP/sfGFP payload)

Depositor

Harris Wang

Article

Ronda et al Nat Methods. 2019 Feb;16(2):167-170. doi

Insert

catP/sfGFP

Use

Synthetic Biology

Expression

Bacterial

Available Since

April 16, 2019

Availability

Academic Institutions and Nonprofits only

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pBbS6c-RFP

Plasmid

#35293

Depositor

Jay Keasling

Article

Lee et al J Biol Eng. 2011 Sep 20;5:12.

Insert

RFP

Use

Synthetic Biology

Expression

Bacterial

Promoter

LlacO-1

Available Since

Feb. 16, 2012

Availability

Academic Institutions and Nonprofits only

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pRB035

Plasmid

#181944

Purpose

aTc-inducible expression of mNeonGreen

Depositor

Jeffrey Tabor

Article

Butcher et al Proc Natl Acad Sci U S A. 2022 Aug 30;11

Insert

mNeonGreen; tetR (AX04_RS23485 Synthetic)

Expression

Bacterial

Promoter

mNG-PLtetO-1; tetR-J23106

Available Since

March 11, 2022

Availability

Academic Institutions and Nonprofits only

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pAC-BETAipi-ispA/dxs

Plasmid

#178302

Purpose

Produces beta-carotene in E. coli; pAC-BETAipi inserted with an operon containing ispA and dxs from E. coli.

Depositor

Yanran Li

Article

Wu et al Sci Adv. 2021 Sep 17;7(38):eabh4048. doi

Inserts

ispA
dxs

Expression

Bacterial

Available Since

Oct. 25, 2022

Availability

Academic Institutions and Nonprofits only

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pTH101

Plasmid

#84459

Purpose

pJB38-NWMN29-30 + SarA_P1-DsRed-Term

Depositor

Reindert Nijland

Article

de Jong et al Sci Rep. 2017 Mar 7;7:43889. doi: 10.103

Insert

SarA_P1-DsRED from pTH1

Available Since

Feb. 17, 2017

Availability

Academic Institutions and Nonprofits only

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EcAvs4

Plasmid

#188794

Purpose

Avs4 from Escherichia coli NCTC11132

Depositor

Feng Zhang

Article

Gao et al Science. 2022 Aug 12;377(6607):eabm4096.

Insert

EcAvs4

Expression

Bacterial

Available Since

Sept. 27, 2022

Availability

Academic Institutions and Nonprofits only

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BBa_J72114-BBa_J72104

Plasmid

#40783

Depositor

Christopher Anderson

Article

Kittleson et al ACS Synth Biol. 2012 Dec 21;1(12):583-9.

Insert

RhaRS + pRhaB + LuxCDABE, VioABCE, LacZ

Use

Luciferase and Synthetic Biology; Phagemid

Expression

Bacterial

Available Since

Jan. 4, 2013

Availability

Academic Institutions and Nonprofits only

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pKmK.I2L

Plasmid

#125031

Purpose

Storage plasmid for K.marxianus left homology arm; type 1 part

Depositor

John Morrissey

Article

Rajkumar et al Front Bioeng Biotechnol. 2019 May 7;7:97

Insert

Left homology arm for targeting integration to chromosome V: 23743..21744

Use

Synthetic Biology

Expression

Yeast

Available Since

May 20, 2019

Availability

Academic Institutions and Nonprofits only

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pBS-SKII-3XHA-eDHFR-NAT

Plasmid

#188926

Purpose

Integration cassette to create C-terminal fusions for fluorescent tagging

Depositor

Stephen Buratowski

Article

Baek et al G3 (Bethesda). 2022 Aug 9. pii: 6659097.

Insert

3XHA-eDHFR-NAT

Expression

Yeast

Available Since

Sept. 8, 2022

Availability

Academic Institutions and Nonprofits only

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