We narrowed to 7,669 results for: Ski

1. pRS415-STE3pr-MFA-Igkappa-FAPoptim-STE3

   Plasmid

   #221112

   Purpose

   FAP tagged STE3 (N-terminally tagged, optimized for yeast expression) under the Ste3 promoter with 2xMYC tag and MFA1 signal sequence to help target construct to the ER

   Depositor

   Allyson O’Donnell

   Article

   Oppenheimer et al Mol Biol Cell. 2024 Jul 1;35(7):mr5. doi

   Insert

   MFA-IgKappa-FAPoptim-STE3
   

   Expression

   Yeast

   Promoter

   STE3

   Available Since

   March 26, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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2. pdnCas3-CDA1

   Plasmid

   #229535

   Purpose

   pMV_hyg encoding dnCas3(H74A+D75A)-CDA1 fusion construct and crRNA expression cassette. Respective crRNA spacer can be introduced via BsmBI restriction cloning

   Depositor

   Kevin Verstrepen

   Article

   Zimmermann et al Nat Commun. 2023 Jun 9;14(1):3389. doi:

   Inserts

   dnCas3
   Cytidine deaminase
   Uracil glycosylase inhibitor
   

   Tags

   XTEN

   Expression

   Bacterial and Yeast

   Mutation

   codon optimized for S.cerevisiae and codon optimi…

   Available Since

   Jan. 23, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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3. pdnCas3-APOBEC1

   Plasmid

   #229537

   Purpose

   pMV_hyg encoding dnCas3(H74A+D75A)-rAPOBEC1 fusion construct and crRNA expression cassette. Respective crRNA spacer can be introduced via BsmBI restriction cloning

   Depositor

   Kevin Verstrepen

   Article

   Zimmermann et al Nat Commun. 2023 Jun 9;14(1):3389. doi:

   Inserts

   rAPOBEC1
   dnCas3
   Uracil glycosylase inhibitor
   

   Tags

   XTEN

   Expression

   Bacterial and Yeast

   Mutation

   codon optimized for S.cerevisiae and codon optimi…

   Available Since

   Jan. 23, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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4. pC130: pAAV.CMV-CasRx-VEGFA sgRNA

   Plasmid

   #203443

   Purpose

   Plasmid expressing active RfxCas13d with VEGFA mRNA targeting gRNA

   Depositor

   Guei-Sheung Liu

   Article

   Kumar et al Proc Natl Acad Sci U S A. 2024 Nov 5;121

   Inserts

   U6-VEGFA sgRNA
   RfxCas13d
   

   Use

   AAV

   Tags

   HA and NLS

   Expression

   Mammalian

   Promoter

   CMV and U6

   Available Since

   Dec. 12, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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5. pM148: pAAV.CMV-Cas13e-NT sgRNA

   Plasmid

   #203446

   Purpose

   Plasmid expressing active RfxCas13d with non-targeting gRNA

   Depositor

   Guei-Sheung Liu

   Article

   Kumar et al Proc Natl Acad Sci U S A. 2024 Nov 5;121

   Inserts

   U6-non targeting (NT) sgRNA
   Cas13e
   

   Use

   AAV

   Tags

   HA and NLS

   Expression

   Mammalian

   Promoter

   CMV and U6

   Available Since

   Dec. 12, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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6. pM149: pAAV.CMV-Cas13e-VEGFA sgRNA

   Plasmid

   #203447

   Purpose

   Plasmid expressing active Cas13e with VEGFA mRNA targeting gRNA

   Depositor

   Guei-Sheung Liu

   Article

   Kumar et al Proc Natl Acad Sci U S A. 2024 Nov 5;121

   Inserts

   U6-VEGFA sgRNA
   Cas13e
   

   Use

   AAV

   Tags

   HA and NLS

   Expression

   Mammalian

   Promoter

   CMV and U6

   Available Since

   Dec. 12, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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7. pM150: pAAV.CMV-Cas13e-VEGFA sgRNA array

   Plasmid

   #203448

   Purpose

   Plasmid expressing active Cas13e with an array of three VEGFA mRNA targeting gRNAs

   Depositor

   Guei-Sheung Liu

   Article

   Kumar et al Proc Natl Acad Sci U S A. 2024 Nov 5;121

   Inserts

   U6-VEGFA sgRNA array
   Cas13e
   

   Use

   AAV

   Tags

   HA and NLS

   Expression

   Mammalian

   Promoter

   CMV and U6

   Available Since

   Dec. 12, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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8. pM155: pAAV.CMV-Cas13e (GenScript CO)-NT sgRNA

   Plasmid

   #203451

   Purpose

   Plasmid expressing active and codon optimised Cas13e with non-targeting gRNA

   Depositor

   Guei-Sheung Liu

   Article

   Kumar et al Proc Natl Acad Sci U S A. 2024 Nov 5;121

   Inserts

   U6-non targeting (NT) sgRNA
   Cas13e
   

   Use

   AAV

   Tags

   HA and NLS

   Expression

   Mammalian

   Mutation

   Codon optimised using GenScript tool

   Promoter

   CMV and U6

   Available Since

   Dec. 12, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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9. pM156: pAAV.CMV-Cas13e (GenScript CO)-VEGFA sgRNA

   Plasmid

   #203452

   Purpose

   Plasmid expressing active and codon optimised Cas13e with VEGFA gRNA

   Depositor

   Guei-Sheung Liu

   Article

   Kumar et al Proc Natl Acad Sci U S A. 2024 Nov 5;121

   Inserts

   U6-VEGFA sgRNA
   Cas13e
   

   Use

   AAV

   Tags

   HA and NLS

   Expression

   Mammalian

   Promoter

   CMV and U6

   Available Since

   Dec. 12, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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10. NEIL1-mCherry

    Plasmid

    #215133

    Purpose

    Expresses NEIL1-mCherry in mammalian cells from a retroviral vector.

    Depositor

    Michele Pagano

    Article

    Rona et al Mol Cell. 2024 Feb 29:S1097-2765(24)0012

    Insert

    NEIL1 (NEIL1 Human)
    

    Use

    Retroviral

    Tags

    mCherry

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    June 17, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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11. pBABE-NLS-HA-TurboID

    Plasmid

    #215073

    Purpose

    Expresses NLS-HA-TurboID in mammalian cells from a retroviral vector.

    Depositor

    Michele Pagano

    Article

    Rona et al Mol Cell. 2024 Feb 29:S1097-2765(24)0012

    Insert

    TurboID (birA )
    

    Use

    Retroviral

    Tags

    NLS-HA

    Expression

    Mammalian

    Promoter

    LTR

    Available Since

    April 8, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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12. mCherry-MUTHY

    Plasmid

    #215132

    Purpose

    Expresses mCherry-MUTHY in mammalian cells from a retroviral vector.

    Depositor

    Michele Pagano

    Article

    Rona et al Mol Cell. 2024 Feb 29:S1097-2765(24)0012

    Insert

    MUTHY (MUTYH Human)
    

    Use

    Retroviral

    Tags

    mCherry

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    April 1, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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13. NEIL2-mCherry

    Plasmid

    #215134

    Purpose

    Expresses NEIL2-mCherry in mammalian cells from a retroviral vector.

    Depositor

    Michele Pagano

    Article

    Rona et al Mol Cell. 2024 Feb 29:S1097-2765(24)0012

    Insert

    NEIL2 (NEIL2 Human)
    

    Use

    Retroviral

    Tags

    mCherry

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    April 1, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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14. 5'-3xFLAG Gatad2a-exon minimal CMV pCDNA5

    Plasmid

    #212051

    Purpose

    LR vector for integration of Gatad2a-exon into N2a FRT rtTA3 expression cells

    Depositor

    Benjamin Blencowe

    Article

    Roth et al Mol Cell. 2023 Dec 7;83(23):4222-4238.e1

    Insert

    Gatad2a-exon (Gatad2a Mouse)
    

    Expression

    Mammalian

    Available Since

    Feb. 7, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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15. 5'-3xFLAG Mef2d-exon minimal CMV pCDNA5

    Plasmid

    #212064

    Purpose

    LR vector for integration of Mef2d-exon into N2a FRT rtTA3 expression cells

    Depositor

    Benjamin Blencowe

    Article

    Roth et al Mol Cell. 2023 Dec 7;83(23):4222-4238.e1

    Insert

    Mef2d-exon (Mef2d Mouse)
    

    Expression

    Mammalian

    Available Since

    Feb. 1, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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16. 5'-3xFLAG L3mbtl3-exon minimal CMV pCDNA5

    Plasmid

    #212058

    Purpose

    LR vector for integration of L3mbtl3-exon into N2a FRT rtTA3 expression cells

    Depositor

    Benjamin Blencowe

    Article

    Roth et al Mol Cell. 2023 Dec 7;83(23):4222-4238.e1

    Insert

    L3mbtl3-exon (L3mbtl3 Mouse)
    

    Expression

    Mammalian

    Available Since

    Jan. 31, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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17. 5'-3xFLAG Mef2a-exon minimal CMV pCDNA5

    Plasmid

    #212062

    Purpose

    LR vector for integration of Mef2a-exon into N2a FRT rtTA3 expression cells

    Depositor

    Benjamin Blencowe

    Article

    Roth et al Mol Cell. 2023 Dec 7;83(23):4222-4238.e1

    Insert

    Mef2a-exon (Mef2a Mouse)
    

    Expression

    Mammalian

    Available Since

    Jan. 31, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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18. 5'-3xFLAG Mef2a+exon minimal CMV pCDNA5

    Plasmid

    #212063

    Purpose

    LR vector for integration of Mef2a+exon(24nt) into N2a FRT rtTA3 expression cells

    Depositor

    Benjamin Blencowe

    Article

    Roth et al Mol Cell. 2023 Dec 7;83(23):4222-4238.e1

    Insert

    Mef2a+exon(24nt) (Mef2a Mouse)
    

    Expression

    Mammalian

    Available Since

    Jan. 31, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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19. 5'-3xFLAG Mef2d+exon minimal CMV pCDNA5

    Plasmid

    #212065

    Purpose

    LR vector for integration of Mef2d+exon(21nt) into N2a FRT rtTA3 expression cells

    Depositor

    Benjamin Blencowe

    Article

    Roth et al Mol Cell. 2023 Dec 7;83(23):4222-4238.e1

    Insert

    Mef2d+exon(21nt) (Mef2d Mouse)
    

    Expression

    Mammalian

    Available Since

    Jan. 31, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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20. LENTICRISPR-LUC7L2_sgRNA1

    Plasmid

    #201602

    Purpose

    CRISPR/Cas9-mediated gene knock-out

    Depositor

    Alexis Jourdain, Vamsi Mootha

    Article

    Jourdain et al Mol Cell. 2021 May 6;81(9):1905-1919.e12

    Insert

    LUC7L2 (LUC7L2 Human)
    

    Use

    CRISPR and Lentiviral

    Available Since

    Dec. 8, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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