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pDEST17-ScChd1[R126A/R130A]

Plasmid

#221197

Purpose

ScChd1-aa118-1274 chromatin remodeler with R126A and R130A mutations

Depositor

Gregory Bowman

Article

Nodelman et al Nat Struct Mol Biol. 2022 Feb;29(2):121-

Insert

ScChd1 (aa118-1274)

Tags

6xHis-tag (Precission protease cleavable)

Expression

Bacterial

Mutation

R126A, R130A

Promoter

T7

Available Since

Aug. 6, 2024

Availability

Academic Institutions and Nonprofits only

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pDEST17-ScChd1[∆ChEx]

Plasmid

#221199

Purpose

ScChd1-aa142-1274 chromatin remodeler with ChEx domain deleted

Depositor

Gregory Bowman

Article

Nodelman et al Nat Struct Mol Biol. 2022 Feb;29(2):121-

Insert

ScChd1 (aa142-1274)

Tags

6xHis-tag (Precission protease cleavable)

Expression

Bacterial

Mutation

deleted amino acids 1-117 and 1275-1468

Available Since

Aug. 6, 2024

Availability

Academic Institutions and Nonprofits only

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pEY140

Plasmid

#223487

Purpose

trp-4 fluorescent neural reporter driving nuclear mCyOFP1 expression (refer to NeuroPAL paper for expression)

Depositor

Oliver Hobert, Eviatar Yemini

Article

Yemini et al Cell. 2021 Jan 7;184(1):272-288.e11. doi

Insert

trp-4 (trp-4 Nematode)

Expression

Worm

Available Since

Aug. 6, 2024

Availability

Academic Institutions and Nonprofits only

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pEY147

Plasmid

#223494

Purpose

unc-86 fluorescent neural reporter driving nuclear mCyOFP1 expression (refer to NeuroPAL paper for expression)

Depositor

Oliver Hobert, Eviatar Yemini

Article

Yemini et al Cell. 2021 Jan 7;184(1):272-288.e11. doi

Insert

unc-86 (unc-86 Nematode)

Expression

Worm

Available Since

Aug. 6, 2024

Availability

Academic Institutions and Nonprofits only

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pEY115

Plasmid

#223462

Purpose

pdfr-1 (3-2K) fluorescent neural reporter driving nuclear mNeptune2.5 expression (refer to NeuroPAL paper for expression)

Depositor

Oliver Hobert, Eviatar Yemini

Article

Yemini et al Cell. 2021 Jan 7;184(1):272-288.e11. doi

Insert

pdfr-1(3-2K) (pdfr-1 Nematode)

Expression

Worm

Available Since

Aug. 6, 2024

Availability

Academic Institutions and Nonprofits only

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pEY145

Plasmid

#223492

Purpose

unc-42 fluorescent neural reporter driving nuclear mCyOFP1 expression (refer to NeuroPAL paper for expression)

Depositor

Oliver Hobert, Eviatar Yemini

Article

Yemini et al Cell. 2021 Jan 7;184(1):272-288.e11. doi

Insert

unc-42 (unc-42 Nematode)

Expression

Worm

Available Since

Aug. 6, 2024

Availability

Academic Institutions and Nonprofits only

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pEY141

Plasmid

#223488

Purpose

trp-4(intron 1) fluorescent neural reporter driving nuclear mCyOFP1 expression (refer to NeuroPAL paper for expression)

Depositor

Oliver Hobert, Eviatar Yemini

Article

Yemini et al Cell. 2021 Jan 7;184(1):272-288.e11. doi

Insert

trp-4(intron 1) (trp-4 Nematode)

Expression

Worm

Available Since

Aug. 6, 2024

Availability

Academic Institutions and Nonprofits only

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pEY146

Plasmid

#223493

Purpose

unc-46 fluorescent neural reporter driving nuclear mTagBFP2 expression (refer to NeuroPAL paper for expression)

Depositor

Oliver Hobert, Eviatar Yemini

Article

Yemini et al Cell. 2021 Jan 7;184(1):272-288.e11. doi

Insert

unc-46 (unc-46 Nematode)

Expression

Worm

Available Since

Aug. 6, 2024

Availability

Academic Institutions and Nonprofits only

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pEY119

Plasmid

#223466

Purpose

ser-2(promCE) fluorescent neural reporter driving nuclear mTagBFP2 expression (refer to NeuroPAL paper for expression)

Depositor

Oliver Hobert, Eviatar Yemini

Article

Yemini et al Cell. 2021 Jan 7;184(1):272-288.e11. doi

Insert

ser-2(promCE) (ser-2 Nematode)

Expression

Worm

Available Since

Aug. 6, 2024

Availability

Academic Institutions and Nonprofits only

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pEY143

Plasmid

#223490

Purpose

unc-31 fluorescent neural reporter driving nuclear TagRFP-T expression (refer to NeuroPAL paper for expression)

Depositor

Oliver Hobert, Eviatar Yemini

Article

Yemini et al Cell. 2021 Jan 7;184(1):272-288.e11. doi

Insert

unc-31 (unc-31 Nematode)

Expression

Worm

Available Since

Aug. 6, 2024

Availability

Academic Institutions and Nonprofits only

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pEY112

Plasmid

#223459

Purpose

oig-1 fluorescent neural reporter driving nuclear mNeptune2.5 expression (refer to NeuroPAL paper for expression)

Depositor

Oliver Hobert, Eviatar Yemini

Article

Yemini et al Cell. 2021 Jan 7;184(1):272-288.e11. doi

Insert

oig-1 (oig-1 Nematode)

Expression

Worm

Available Since

Aug. 6, 2024

Availability

Academic Institutions and Nonprofits only

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pEY113

Plasmid

#223460

Purpose

osm-10 fluorescent neural reporter driving nuclear mTagBFP2 expression (refer to NeuroPAL paper for expression)

Depositor

Oliver Hobert, Eviatar Yemini

Article

Yemini et al Cell. 2021 Jan 7;184(1):272-288.e11. doi

Insert

osm-10 (osm-10 Nematode)

Expression

Worm

Available Since

Aug. 6, 2024

Availability

Academic Institutions and Nonprofits only

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pEY133

Plasmid

#223480

Purpose

srz-45 fluorescent neural reporter driving nuclear mNeptune2.5 expression (refer to NeuroPAL paper for expression)

Depositor

Oliver Hobert, Eviatar Yemini

Article

Yemini et al Cell. 2021 Jan 7;184(1):272-288.e11. doi

Insert

srz-45 (srz-45 Nematode)

Expression

Worm

Available Since

Aug. 6, 2024

Availability

Academic Institutions and Nonprofits only

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pEY130

Plasmid

#223477

Purpose

srx-113 fluorescent neural reporter driving nuclear mNeptune2.5 expression (refer to NeuroPAL paper for expression)

Depositor

Oliver Hobert, Eviatar Yemini

Article

Yemini et al Cell. 2021 Jan 7;184(1):272-288.e11. doi

Insert

srx-113 (srx-113 Nematode)

Expression

Worm

Available Since

Aug. 6, 2024

Availability

Academic Institutions and Nonprofits only

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MAC3-CDK8

Plasmid

#222609

Purpose

Expression of MAC3-tagged CDK8

Depositor

Markku Varjosalo

Article

Liu et al Methods Mol Biol. 2023;2690:281-297. doi

Insert

CDK8 (CDK8 Human)

Tags

MAC3

Expression

Bacterial and Mammalian

Promoter

CMV

Available Since

Aug. 6, 2024

Availability

Industry, Academic Institutions, and Nonprofits

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pEY131

Plasmid

#223478

Purpose

srx-105 fluorescent neural reporter driving nuclear CyOFP1 expression (refer to NeuroPAL paper for expression)

Depositor

Oliver Hobert, Eviatar Yemini

Article

Yemini et al Cell. 2021 Jan 7;184(1):272-288.e11. doi

Insert

srx-105 (srx-105 Nematode)

Expression

Worm

Available Since

Aug. 6, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pEY137

Plasmid

#223484

Purpose

tax-2 fluorescent neural reporter driving nuclear mTagBFP2 expression (refer to NeuroPAL paper for expression)

Depositor

Oliver Hobert, Eviatar Yemini

Article

Yemini et al Cell. 2021 Jan 7;184(1):272-288.e11. doi

Insert

tax-2 (tax-2 Nematode)

Expression

Worm

Available Since

Aug. 6, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pEY138

Plasmid

#223485

Purpose

tol-1(3k) fluorescent neural reporter driving nuclear mNeptune2.5 expression (refer to NeuroPAL paper for expression)

Depositor

Oliver Hobert, Eviatar Yemini

Article

Yemini et al Cell. 2021 Jan 7;184(1):272-288.e11. doi

Insert

tol-1(3k) (tol-1 Nematode)

Expression

Worm

Available Since

Aug. 5, 2024

Availability

Academic Institutions and Nonprofits only

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pEY144

Plasmid

#223491

Purpose

unc-39(3k) fluorescent neural reporter driving nuclear mCyOFP1 expression (refer to NeuroPAL paper for expression)

Depositor

Oliver Hobert, Eviatar Yemini

Article

Yemini et al Cell. 2021 Jan 7;184(1):272-288.e11. doi

Insert

unc-39 (3k) (unc-39 Nematode)

Expression

Worm

Available Since

Aug. 5, 2024

Availability

Academic Institutions and Nonprofits only

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pGM258

Plasmid

#220176

Purpose

Expresses exogenous C-terminal mutant TTC1 (I271D)

Depositor

Jonathan Weissman

Article

Muthukumar et al Mol Cell. 2024 Mar 21;84(6):1101-1119.e9

Insert

TTC1 (TTC1 Human)

Use

Lentiviral

Tags

3xFLAG

Mutation

I271D

Available Since

June 7, 2024

Availability

Academic Institutions and Nonprofits only

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