We narrowed to 23,651 results for: crispr

1. TRIM24 gRNA (BRDN0001146855)

   Plasmid

   #77886

   Purpose

   3rd generation lentiviral gRNA plasmid targeting human TRIM24

   Depositor

   John Doench, David Root

   Article

   Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

   Insert

   TRIM24 (TRIM24 Human)
   

   Use

   CRISPR and Lentiviral

   Expression

   Mammalian

   Promoter

   hU6

   Available Since

   July 6, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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2. TRIM33 gRNA (BRDN0001162242)

   Plasmid

   #77099

   Purpose

   3rd generation lentiviral gRNA plasmid targeting human TRIM33

   Depositor

   John Doench, David Root

   Article

   Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

   Insert

   TRIM33 (TRIM33 Human)
   

   Use

   CRISPR and Lentiviral

   Expression

   Mammalian

   Promoter

   hU6

   Available Since

   July 6, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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3. UAS-u(S)Cas9

   Plasmid

   #127383

   Purpose

   Expresses Cas9 at high level

   Depositor

   Michael Boutros

   Article

   Port et al Elife. 2020 Feb 13;9. pii: 53865. doi: 1

   Insert

   uORF(S)-Cas9
   

   Use

   CRISPR

   Expression

   Insect

   Promoter

   UAST

   Available Since

   June 29, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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4. PB_EF1a-CasRx-msfGFP-2A-Blast

   Plasmid

   #226010

   Purpose

   CasRx protein for RNA targeting in piggyBac backbone

   Depositor

   Silvana Konermann

   Article

   Wei et al Cell Syst. 2023 Dec 20;14(12):1087-1102.

   Insert

   CasRx RNA-targeting nuclease
   

   Use

   CRISPR

   Tags

   msfGFP

   Expression

   Mammalian

   Available Since

   Dec. 4, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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5. pHelper-T7IS1

   Plasmid

   #140631

   Purpose

   Expresses ShCAST under the T7 promoter and an empty sgRNA

   Depositor

   Sheng Yang

   Article

   Zhang et al ACS Synth Biol. 2020 Jun 17. doi: 10.102

   Insert

   ShTnsB, ShTnsC, ShTniQ, ShCas12k, sgRNA targeting IS1 (Escherichia coli)
   

   Use

   CRISPR; Transposon

   Expression

   Bacterial

   Available Since

   July 16, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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6. pYPQ292 (AaCas12b)-D570A-TV-MS2-VPR

   Plasmid

   #136382

   Purpose

   CRISPRa Gateway entry clone for catalytically dead AaCas12b (D570A) fused with TV activation system, followed by T2A and MCP-VPR fusion protein

   Depositor

   Yiping Qi

   Article

   Ming et al Nat Plants. 2020 Mar;6(3):202-208. doi:

   Insert

   AaCas12b (D570A)-TV-T2A-MCP-VPR
   

   Use

   CRISPR

   Expression

   Plant

   Mutation

   D570A

   Available Since

   Feb. 18, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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7. pJZC33

   Plasmid

   #62330

   Purpose

   sgRNA + 2x MS2 with MCP-VP64 effector for mammalian cells

   Depositor

   Wendell Lim, Stanley Qi

   Article

   Zalatan et al Cell. 2014 Dec 18. pii: S0092-8674(14)01

   Inserts

   sgRNA + 2x MS2 binding module
   MCP-VP64
   

   Use

   Lentiviral

   Tags

   VP64

   Expression

   Mammalian

   Mutation

   Targets Tet3G, sequence: GTACGTTCTCTATCACTGATA

   Promoter

   CMV and U6

   Available Since

   April 2, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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8. TRIM33 gRNA (BRDN0001162291)

   Plasmid

   #77097

   Purpose

   3rd generation lentiviral gRNA plasmid targeting human TRIM33

   Depositor

   John Doench, David Root

   Article

   Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

   Insert

   TRIM33 (TRIM33 Human)
   

   Use

   CRISPR and Lentiviral

   Expression

   Mammalian

   Promoter

   hU6

   Available Since

   July 6, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. TRIM33 gRNA (BRDN0001162239)

   Plasmid

   #77098

   Purpose

   3rd generation lentiviral gRNA plasmid targeting human TRIM33

   Depositor

   John Doench, David Root

   Article

   Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

   Insert

   TRIM33 (TRIM33 Human)
   

   Use

   CRISPR and Lentiviral

   Expression

   Mammalian

   Promoter

   hU6

   Available Since

   July 6, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pSLPB2B-ecDHFR-SpdCas9-tagRFPt-P2A-tagBFP-PGK-Blasticidin

    Plasmid

    #187950

    Purpose

    ecDHFR degron-tagged dCas9 fused with tagRFPt, P2A site and tagBFP, expressed under EF1-alpha promoter in a piggyBac plasmid. Expresses Blasticidin resistance under PGK promoter.

    Depositor

    Edda Schulz

    Article

    Noviello et al Nat Commun. 2023 Jun 3;14(1):3225. doi:

    Insert

    ecDHFR-SpdCas9-tagRFPt-P2A-tagBFP
    

    Use

    CRISPR and Synthetic Biology

    Tags

    GGS linker

    Expression

    Mammalian

    Available Since

    Oct. 14, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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11. RPS6KA1 gRNA (BRDN0001145974)

    Plasmid

    #75497

    Purpose

    3rd generation lentiviral gRNA plasmid targeting human RPS6KA1

    Depositor

    John Doench, David Root

    Article

    Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

    Insert

    RPS6KA1 (RPS6KA1 Human)
    

    Use

    CRISPR and Lentiviral

    Expression

    Mammalian

    Promoter

    hU6

    Available Since

    July 6, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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12. PIK3C2B gRNA (BRDN0001145952)

    Plasmid

    #76714

    Purpose

    3rd generation lentiviral gRNA plasmid targeting human PIK3C2B

    Depositor

    John Doench, David Root

    Article

    Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

    Insert

    PIK3C2B (PIK3C2B Human)
    

    Use

    CRISPR and Lentiviral

    Expression

    Mammalian

    Promoter

    hU6

    Available Since

    July 6, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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13. pROS11

    Plasmid

    #107925

    Purpose

    amdS based Yeast/E.coli shuttle vector designed to clone and express Cas9 programming spacer

    Depositor

    Jean-Marc Daran

    Article

    Mans et al FEMS Yeast Res. 2015 Mar;15(2). pii: fov

    Insert

    gRNA-CAN1.Y, gRNA-ADE2.Y
    

    Use

    CRISPR

    Expression

    Yeast

    Available Since

    April 18, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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14. TRIM24 gRNA (BRDN0001145637)

    Plasmid

    #77887

    Purpose

    3rd generation lentiviral gRNA plasmid targeting human TRIM24

    Depositor

    John Doench, David Root

    Article

    Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

    Insert

    TRIM24 (TRIM24 Human)
    

    Use

    CRISPR and Lentiviral

    Expression

    Mammalian

    Promoter

    hU6

    Available Since

    July 6, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pMEL16

    Plasmid

    #107922

    Purpose

    HIS3 based Yeast/E.coli shuttle vector designed to clone and express Cas9 programming spacer

    Depositor

    Jean-Marc Daran

    Article

    Mans et al FEMS Yeast Res. 2015 Mar;15(2). pii: fov

    Insert

    gRNA-CAN1.Y
    

    Use

    CRISPR

    Expression

    Yeast

    Available Since

    April 18, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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16. pJEP302-pAAV-CMV-dSaCas9-KRAB-pA

    Plasmid

    #113677

    Purpose

    A CMV driven de-catalyzed SaCas9 fused to KRAB domain for inhibition of transcription in targeted region.

    Depositor

    Jonathan Ploski

    Article

    Kumar et al Front Mol Neurosci. 2018 Nov 13;11:413.

    Insert

    de-catalyzed SaCas9
    

    Use

    AAV and CRISPR

    Tags

    KRAB and NLS

    Available Since

    Feb. 14, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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17. pSLPB2B-ER50-SpdCas9-tagRFPt-P2A-tagBFP-PGK-Blasticidin

    Plasmid

    #187948

    Purpose

    ER50 degron-tagged dCas9 fused with tagRFPt, P2A site and tagBFP, expressed under EF1-alpha promoter in a piggyBac plasmid. Expresses Blasticidin resistance under PGK promoter.

    Depositor

    Edda Schulz

    Article

    Noviello et al Nat Commun. 2023 Jun 3;14(1):3225. doi:

    Insert

    ER50-SpdCas9-tagRFPt-P2A-tagBFP
    

    Use

    CRISPR and Synthetic Biology

    Tags

    GGS linker

    Expression

    Mammalian

    Mutation

    6 mutations, T371A, L384M, M421G, G521R, Y537S, N…

    Available Since

    Oct. 14, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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18. TK1 gRNA (BRDN0001145069)

    Plasmid

    #76424

    Purpose

    3rd generation lentiviral gRNA plasmid targeting human TK1

    Depositor

    John Doench, David Root

    Article

    Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

    Insert

    TK1 (TK1 Human)
    

    Use

    CRISPR and Lentiviral

    Expression

    Mammalian

    Promoter

    hU6

    Available Since

    July 6, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. RPS6KA1 gRNA (BRDN0001148103)

    Plasmid

    #75498

    Purpose

    3rd generation lentiviral gRNA plasmid targeting human RPS6KA1

    Depositor

    John Doench, David Root

    Article

    Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

    Insert

    RPS6KA1 (RPS6KA1 Human)
    

    Use

    CRISPR and Lentiviral

    Expression

    Mammalian

    Promoter

    hU6

    Available Since

    July 6, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pNOC_hfnCas12a_NR1_NR2

    Plasmid

    #176252

    Purpose

    pNOC episomal plasmid harboring the humanized fnCas12a gene sequence tagged with Nlux and CRISPR array with spacers 1 and 2 targeting the Nitrate reductase gene of N. oceanica IMET1 separated by full

    Depositor

    John van der Oost

    Article

    Naduthodi et al ACS Synth Biol. 2021 Dec 17;10(12):3369-

    Insert

    humanized fnCas12a
    

    Use

    CRISPR, Luciferase, and Synthetic Biology ; Expre…

    Tags

    luciferase

    Available Since

    Nov. 3, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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