We narrowed to 44,309 results for: tra

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nAChR alpha4 YFP

Plasmid

#15245

Depositor

Henry Lester

Article

Nashmi et al J Neurosci. 2003 Dec 17. 23(37):11554-67

Insert

alpha4-YFP-M (Chrna4 Mouse)

Tags

YFP

Expression

Mammalian

Available Since

July 6, 2007

Availability

Academic Institutions and Nonprofits only
TRIPZ-bioGEF-UBwt-PURO

Plasmid

#208045

Purpose

Enables inducible expression of low-affinity Avitag-Ubwt; to perform bioE3; selection with puromycin

Depositor

Rosa Barrio, James Sutherland

Article

Barroso-Gomila et al Nat Commun. 2023 Nov 23;14(1):7656. doi:

Insert

Ub (UBC Human)

Use

Lentiviral

Tags

Low-affinity Avitag

Promoter

tetO/UBC

Available Since

Nov. 29, 2023

Availability

Academic Institutions and Nonprofits only

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pCDFDuet-nsp7-nsp8

Plasmid

#159092

Purpose

Coexpression construct of Nsp7 with an N-terminal His-tag and Nsp8

Depositor

Seth Darst

Article

Chen et al Cell. 2020 Sep 17;182(6):1560-1573.e13.

Inserts

NSP7
NSP8

Tags

His

Expression

Bacterial

Promoter

T7

Available Since

Sept. 28, 2020

Availability

Academic Institutions and Nonprofits only

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pActin-miRFP670nano

Plasmid

#127428

Purpose

Human beta-actin labeling with near-infrared fluorescent protein miRFP670nano

Depositor

Vladislav Verkhusha

Article

Oliinyk et al Nat Commun. 2019 Jan 17;10(1):279. doi:

Insert

miRFP670nano-human beta-actin

Tags

miRFP670nano

Expression

Mammalian

Promoter

CMV

Available Since

July 17, 2019

Availability

Academic Institutions and Nonprofits only

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pCAGGS-CD4-myc

Plasmid

#58537

Purpose

Expresses human CD4 with a C-terminal myc tag in mammalian cells

Depositor

Jacob Yount

Article

Chesarino et al J Biol Chem. 2014 Apr 25;289(17):11986-9

Insert

CD4 (CD4 Human)

Tags

myc

Expression

Mammalian

Available Since

Sept. 16, 2014

Availability

Academic Institutions and Nonprofits only
pBOBI-FLuc

Plasmid

#170674

Purpose

Express firefly luciferase. Used in lentivirus-based SARS-CoV-2 related (MERS) pseudovirus assay.

Depositor

David Nemazee

Article

Rogers et al Science. 2020 Aug 21;369(6506):956-963.

Insert

luc

Use

Lentiviral and Luciferase

Expression

Mammalian

Promoter

CMV-F

Available Since

July 19, 2021

Availability

Academic Institutions and Nonprofits only

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pT2KXIGdeltaIn-MCS-Avi-EGFP-rpl10a

Plasmid

#58380

Purpose

tol2 transgenesis for biotin based TRAP - Avi tagged Ribosome

Depositor

Didier Stainier

Article

Housley et al Development. 2014 Oct;141(20):3988-93. d

Insert

avi-EGFP-rpl10a

Tags

Avi Tag and EGFP

Available Since

Aug. 4, 2014

Availability

Academic Institutions and Nonprofits only

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PKC eta DN

Plasmid

#21246

Depositor

Bernard Weinstein

Article

Soh et al J Biol Chem. 2003 Sep 5. 278(36):34709-1

Insert

PKC eta (Prkch Mouse)

Tags

HA

Expression

Mammalian

Mutation

K384R

Available Since

Aug. 13, 2009

Availability

Academic Institutions and Nonprofits only
pGEX-BAIAP2 wt

Plasmid

#31675

Depositor

Anne Brunet

Article

Banko et al Mol Cell. 2011 Dec 23;44(6):878-92. Epub

Insert

BAI1-associated protein 2 (BAIAP2 Human)

Tags

GST

Expression

Bacterial

Available Since

April 11, 2012

Availability

Academic Institutions and Nonprofits only

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SV40 basal promoter-GFP-IRES-AP

Plasmid

#18807

Depositor

Connie Cepko

Article

Kim et al J Neurosci. 2008 Jul 30. 28(31):7748-64.

Insert

SV40 basal promoter-GFP-IRES-AP

Expression

Mammalian

Available Since

Aug. 13, 2008

Availability

Academic Institutions and Nonprofits only
pME-Kaede

Plasmid

#132972

Purpose

full-length of photoconvertible kaede with stop codon in pmE Gateway vector

Depositor

Nathan Lawson

Article

Shin et al Dev Cell. 2019 Nov 4;51(3):374-386.e5. d

Insert

Kaede

Use

Gateway vector

Available Since

Dec. 4, 2019

Availability

Academic Institutions and Nonprofits only

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pRBBm34

Plasmid

#48114

Purpose

Expression of the gfp gene under control of the native xylose inducible promoter of Bacillus megaterium

Depositor

Dieter Jahn

Article

Biedendieck et al J Biotechnol. 2007 Dec 1;132(4):426-30.

Insert

gfp

Use

Synthetic Biology

Expression

Bacterial

Available Since

Oct. 11, 2013

Availability

Academic Institutions and Nonprofits only

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Beta-2-adrenergic receptor-CFP

Plasmid

#55794

Purpose

Beta-2-adrenergic receptor tagged with ECFP on carboxyl terminus

Depositor

Catherine Berlot

Article

Hynes et al J Biol Chem. 2004 Oct 15;279(42):44101-1

Insert

Beta-2-adrenergic receptor-ECFP (ADRB2 Aequorea victoria, Human)

Tags

ECFP

Expression

Mammalian

Promoter

CMV

Available Since

Aug. 18, 2015

Availability

Academic Institutions and Nonprofits only

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bRA90

Plasmid

#100951

Purpose

Cas9 driven by PGK1 promoter. gRNA can be cloned into the BplI sites. LEU2 marker

Depositor

James Haber

Article

Anand et al Nature. 2017 Apr 20;544(7650):377-380. d

Type

Empty backbone

Use

CRISPR and Synthetic Biology

Expression

Yeast

Available Since

Sept. 11, 2017

Availability

Academic Institutions and Nonprofits only

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TNFa-GFP

Plasmid

#163660

Purpose

Expresses GFP tagged TNFa in mammalian cells

Depositor

Lei Lu

Article

Sun et al J Cell Sci. 2021 Oct 15;134(20). pii: 27

Insert

tumor necrosis factor (TNF Human)

Tags

GFP

Expression

Mammalian

Available Since

Nov. 24, 2021

Availability

Academic Institutions and Nonprofits only

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pHS0812 hU6-BpiI-OgeuIscB omegaRNA scaffold

Plasmid

#176541

Purpose

human U6-driven expression of OgeuIscB omegaRNA scaffold with BpiI site for guide cloning

Depositor

Feng Zhang

Article

Altae-Tran et al Science. 2021 Oct;374(6563):57-65. doi:

Insert

OgeuIscB omegaRNA scaffold

Expression

Mammalian

Available Since

Oct. 18, 2021

Availability

Academic Institutions and Nonprofits only

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pAAV-pCMV-dSaCas9-VP64-pU6-sgRNA

Plasmid

#158990

Purpose

Vector G encodes pAAV-pCMV-dSaCas9-VP64-spA-pU6-sgRNA (BsaI) transgenes for AAV packaging and expression of CRISPR activator in mammalian cells

Depositor

Chung Tin

Article

Lau et al Mol Ther Nucleic Acids. 2019 Jun 7;16:63

Insert

dSaCas9-VP64

Use

AAV and CRISPR

Expression

Mammalian

Promoter

pCMV

Available Since

Aug. 29, 2020

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-GGamma1-GFP2

Plasmid

#140989

Purpose

Encodes a G gamma subunit (GNGT1) containing GFP2 as an optimal component of a BRET2 biosensor for studying heterotrimeric G protein dissociation

Depositor

Bryan Roth

Article

Olsen et al Nat Chem Biol. 2020 May 4. pii: 10.1038/

Insert

GGamma1-GFP2 (GNGT1 Human)

Tags

GFP2 and GSAG linker

Expression

Mammalian

Promoter

CMV

Available Since

June 26, 2020

Availability

Academic Institutions and Nonprofits only

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pEGFP Tensin1

Plasmid

#105297

Purpose

To study localization and function of Tensin1

Depositor

David Critchley, Kenneth Yamada

Article

Clark et al J Cell Biochem. 2010 Mar 1;109(4):808-17

Insert

Tensin1 (TNS1 Human)

Tags

EGFP

Expression

Mammalian

Available Since

Feb. 28, 2018

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1(-) FLIPQTV3.0 100micro

Plasmid

#63730

Purpose

FLIPQTV3.0 glutamine sensor with D157N mutation

Depositor

Sakiko Okumoto

Article

Gruenwald et al PLoS One. 2012;7(6):e38591. doi: 10.1371

Insert

FLIPQTV3.0 sensor, 100microM affinity

Expression

Mammalian

Mutation

D157N in glutamine binding domain

Available Since

Nov. 18, 2015

Availability

Academic Institutions and Nonprofits only

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