We narrowed to 152,943 results for: DES

1. alpha catenin conformation sensor

   Plasmid

   #71709

   Purpose

   This alpha catenin biosensor undergoes a change in FRET upon mechanical perturbation of the alpha catenin conformation.

   Depositor

   Deborah Leckband

   Article

   Kim et al Curr Biol. 2015 Jan 19;25(2):218-24. doi

   Insert

   alpha-catenin FRET Sensor
   

   Use

   Tags

   ECFP and YPET

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   April 19, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pENTR-eGFP-attP(bxb)-*BsdR

   Plasmid

   #183751

   Purpose

   Bxb1 landing pad cassette.

   Depositor

   Richard Davis

   Article

   Blanch-Asensio et al Cell Rep Methods. 2022 Sep 22;2(10):1003

   Insert

   loxP-eGFP-attP-Bsd-lox251
   

   Use

   Cre/Lox and Synthetic Biology

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Sept. 13, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pBR_attB(bxb)_ccdB_lox

   Plasmid

   #183763

   Purpose

   Bxb1-specific donor plasmid for the cloning of multiple inserts by Golden Gate assembly (BpiI)

   Depositor

   Richard Davis

   Article

   Blanch-Asensio et al Cell Rep Methods. 2022 Sep 22;2(10):1003

   Insert

   ccdB
   

   Use

   Cre/Lox and Synthetic Biology

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Sept. 13, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pKLV-EF1amCherry-W

   Plasmid

   #159295

   Purpose

   Lentiviral vector expressing mCherry driven by human EF1a promoter

   Depositor

   Kosuke Yusa

   Article

   Au et al Leukemia. 2021 Apr;35(4):1012-1022. doi:

   Insert

   mCherry
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   human EF1a promoter

   Available since

   Sept. 23, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. PLKO-mcherry-luc-puro-Renilla cDNA

   Plasmid

   #29783

   Depositor

   Carl Novina

   Article

   Levy et al Mol Cell. 2010 Dec 10. 40(5):841-9.

   Insert

   Renilla
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   June 1, 2011

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pENTR223-EBNA2

   Plasmid

   #195815

   Purpose

   Gateway Entry Clone of Epstein-Barr virus EBNA2

   Depositor

   Eric Johannsen

   Article

   Calderwood et al Proc Natl Acad Sci U S A. 2007 May 1;104

   Insert

   EBNA2 (EBNA-2 Epstein-Barr virus)
   

   Use

   Gateway entry vector

   Tags

   Expression

   Mutation

   Promoter

   Available since

   May 19, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pcDNA3-FLAG-HA-CAD

   Plasmid

   #46238

   Purpose

   For expression of HA and FLAG-tagged murine CAD in mammalian cells

   Depositor

   Brendan Manning

   Article

   Ben-Sahra et al Science. 2013 Mar 15;339(6125):1323-8. d

   Insert

   carbamoyl-phosphate synthetase 2, aspartate transcarbamylase, and dihydroorotase (Cad Mouse)
   

   Use

   Tags

   FLAG and HA

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Nov. 22, 2013

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. 1255_pAAV-U6-SA-BbsI-MluI-gRNA-CB-SACas9-HA-OLLAS-spA

   Plasmid

   #109320

   Purpose

   Plasmid for AAV SaCas9 Mammalian Expression with a BbsI cloning site for a gRNA

   Depositor

   William Lagor

   Article

   Jarrett et al Arterioscler Thromb Vasc Biol. 2018 Jul

   Type

   Empty backbone

   Use

   AAV and CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   June 28, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pAAV_CaMKIIa_SERT_EYFP

   Plasmid

   #198737

   Purpose

   Serotonin transporter with EYFP fluorescent protein driven by CaMKIIa promoter on a AAV vector

   Depositor

   Lin Tian

   Article

   Vargas et al Science. 2023 Feb 17;379(6633):700-706.

   Insert

   Serotonin transporter and yellow fluorescent protein
   

   Use

   AAV

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CaMKIIa

   Available since

   March 27, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pCSF107mT-GATEWAY-3'-FLAG

    Plasmid

    #67619

    Purpose

    A pCS2 based vector to generate a 3'-Flag tag using GATEWAY cloning technology

    Depositor

    Todd Stukenberg

    Article

    Grant et al Dev Biol. 2015 Dec 15;408(2):345-57. doi

    Type

    Empty backbone

    Use

    In vitro transcription or translation off of an s…

    Tags

    C-terminal Flag

    Expression

    Mammalian

    Mutation

    Promoter

    CMV and SP6

    Available since

    Aug. 5, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. Bxb1_AJMA

    Plasmid

    #183760

    Purpose

    Bxb1-specific donor plasmid for integrating transcription units for expressing ASAP2f (2 copies), jRCaMP1b and membrane-localised miRFP703

    Depositor

    Richard Davis

    Article

    Blanch-Asensio et al Cell Rep Methods. 2022 Sep 22;2(10):1003

    Inserts

    ASAP2f
    jRCaMP1b
    miRFP703
    ASAP2f
    

    Use

    Tags

    Lck and NES

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Sept. 13, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pENTR223-EBNA3C

    Plasmid

    #198576

    Purpose

    Gateway Entry Clone

    Depositor

    Eric Johannsen

    Article

    Calderwood et al Proc Natl Acad Sci U S A. 2007 May 1;104

    Insert

    EBNA3C (EBNA-3B/EBNA-3C Epstein-Barr virus)
    

    Use

    Gateway entry vector

    Tags

    Expression

    Mutation

    None (wildtype)

    Promoter

    Available since

    May 19, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. EGFP gRNA (BRDN0000562805)

    Plasmid

    #80035

    Purpose

    3rd generation lentiviral gRNA plasmid targeting EGFP

    Depositor

    David Root, John Doench

    Article

    Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

    Insert

    EGFP
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    hU6

    Available since

    July 25, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pD3sE_SC12

    Plasmid

    #219720

    Purpose

    DV3 E dimer with fusion loop mutation and increased expression

    Depositor

    Brian Kuhlman

    Article

    Phan et al J Biol Chem. 2022 Jul;298(7):102079. doi

    Insert

    DV3 sE SC12
    

    Use

    Tags

    8xHis and human serum albumin (HSA)

    Expression

    Mammalian

    Mutation

    G106D, A257W, T260R, F277W, A278P, G29K, T33V, A3…

    Promoter

    Available since

    May 17, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pLYS1-MICU1-Flag

    Plasmid

    #50058

    Purpose

    Expression of human MICU1 with Flag tag in mammalian cells

    Depositor

    Vamsi Mootha

    Article

    Sancak et al Science. 2013 Nov 14.

    Insert

    MICU1 (isoform 2) (MICU1 Human)
    

    Use

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    *

    Promoter

    Available since

    May 30, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pENTR223-EBNA3B

    Plasmid

    #198574

    Purpose

    Gateway Entry Clone

    Depositor

    Eric Johannsen

    Article

    Calderwood et al Proc Natl Acad Sci U S A. 2007 May 1;104

    Insert

    EBNA3B (EBNA-3B/EBNA-3C Epstein-Barr virus)
    

    Use

    Gateway entry vector

    Tags

    Expression

    Mutation

    None (wildtype)

    Promoter

    Available since

    May 19, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pENTR223-EBNA3A

    Plasmid

    #198575

    Purpose

    Gateway Entry Clone

    Depositor

    Eric Johannsen

    Article

    Calderwood et al Proc Natl Acad Sci U S A. 2007 May 1;104

    Insert

    EBNA3A (EBNA-3A Epstein-Barr virus)
    

    Use

    Gateway entry vector

    Tags

    Expression

    Mutation

    None (wildtype)

    Promoter

    Available since

    May 19, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pIND(V5)EcoDam

    Plasmid

    #59201

    Purpose

    Mammalian DamID vector used to fuse gene of interest to V5-tagged E.coli Dam. Can also express Dam alone as negative control.

    Depositor

    Bas van Steensel

    Article

    Vogel et al Genome Res. 2006 Dec;16(12):1493-504. Ep

    Type

    Empty backbone

    Use

    Damid

    Tags

    Dam (DNA adenine methyltransferase) and V5

    Expression

    Mammalian

    Mutation

    Promoter

    Heat Shock Minimal Promoter

    Available since

    June 14, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pGTM_COV2_NSP5_004_SUMO

    Plasmid

    #190062

    Purpose

    Expresses SARS-CoV-2 3CLpro for the expression of the active mature protease

    Depositor

    Gaetano Montelione

    Article

    Mazzei et al STAR Protoc. 2023 May 5;4(2):102326. doi

    Insert

    3CLpro (ORF1ab SARS-CoV-2)
    

    Use

    Tags

    6X-His-Sumo

    Expression

    Bacterial

    Mutation

    Promoter

    T7 lac

    Available since

    June 3, 2024

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

20. pBAD_sfGFP3TCG4TAG

    Plasmid

    #174518

    Purpose

    Recoded sfGFP reporter with the codons TCG and TAG inserted at positions 3 and 4, respectively.

    Depositor

    Jason W Chin

    Article

    Robertson et al Science. 2021 Jun 4;372(6546):1057-1062.

    Insert

    sfGFP
    

    Use

    Tags

    6 x His

    Expression

    Bacterial

    Mutation

    Recoded. TCG and TAG codons were introduced in po…

    Promoter

    araBAD

    Available since

    Oct. 18, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge