Skip to main content
Addgene
Open Menu

Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

Information

In Category

Filter By

We narrowed to 14,065 results for: LEA

Clear keywords
Showing: 5321 - 5340 of 14065 results

  1. pCEP4-HLA-cmyc-optiT1R3 a21-852

    Plasmid
    #113948
    Purpose
    mammalian expression plasmid for c-myc-tagged human codon-optimized human T1R3 with signal peptide of HLA class I histocompatibility antigen A-2 alpha chain
    Depositor
    Erik Procko
    Publication
    Park et al J Biol Chem. 2019 Feb 5. pii: RA118.0061
    Insert
    T1R3 (TAS1R3 Human)
    Tags
    Signal/leader sequence from HLA class I histocomp…
    Expression
    Mammalian
    Mutation
    residues 21-852
    Promoter
    CMV
    Available since
    Feb. 8, 2019
    Availability
    Academic Institutions and Nonprofits only
    113948-plasmid-map-sequence-id-215162

    Enlarge

  2. pET24a-VHH-2xTS

    Plasmid
    #109419
    Purpose
    Bacterial expression of a functionalized anti-GFP (VHH) nanobody fused to two tyrosine sulfation (TS) motifs. VHH-2xTS also contains a T7, HA, BAP and His6 epitope
    Depositor
    Martin Spiess
    Publication
    Buser et al Proc Natl Acad Sci U S A. 2018 Jul 3;115
    Insert
    anti-GFP nanobody fused to a T7, TS, HA, BAP and His6 epitope
    Tags
    BAP, HA, His6, T7, and Tyrosine sulfation (TS) se…
    Expression
    Bacterial
    Promoter
    T7
    Available since
    June 19, 2018
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    109419-plasmid-map-sequence-id-211590

    Enlarge

  6. Rat Alpha2 delta-1 mutMIDAS pMT2

    Plasmid
    #58730
    Purpose
    expression of rat alpha2 delta-1 with MIDAS site mutations in mammalian cells
    Depositor
    Annette Dolphin
    Publication
    Hoppa et al Nature. 2012 May 13;486(7401):122-5. doi
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    alpha2 delta-1 (Cacna2d1 Rat)
    Expression
    Mammalian
    Mutation
    MIDAS site mutations (D259VSGS to AVAGA)
    Promoter
    SV40
    Available since
    Oct. 3, 2014
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    58730-plasmid-map-sequence-id-90812

    Enlarge

  7. BC-Sig-GPRC

    Plasmid
    #44201
    Depositor
    Lei Xu
    Insert
    human GPR56 C-terminal fragment with signal peptide (ADGRG1 Human)
    Use
    Retroviral
    Tags
    none
    Expression
    Mammalian
    Mutation
    The C-terminal fragment of GPR56, starting at am…
    Promoter
    LTR
    Available since
    July 12, 2013
    Availability
    Academic Institutions and Nonprofits only
    44201-plasmid-map-sequence-id-60060

    Enlarge

  12. pB-Tet-On-HTR6-RhoA sensor

    Plasmid
    #189614
    Purpose
    Tet-On cilia-targeted RhoA sensor (Xlone piggybac back-bone); HTR6-fused with a sGFP-mScarlet-I based sensor
    Depositor
    David Clapham
    Publication
    Sheu et al Cell. 2022 Sep 1;185(18):3390-3407.e18.
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    HTR6 (HTR6 Human)
    Tags
    GFP and mScarlet
    Expression
    Mammalian
    Promoter
    Tet3G
    Available since
    March 29, 2023
    Availability
    Academic Institutions and Nonprofits only
    189614-plasmid-map-sequence-id-387472

    Enlarge

  16. pGBW-m4258306

    Plasmid
    #154795
    Purpose
    Mammalian expression plasmid for SARS-CoV-2 E (envelope)
    Depositor
    Ginkgo Bioworks, Benjie Chen
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    SARS-CoV-2 E (envelope) (E Severe acute respiratory syndrome coronavirus 2)
    Tags
    Cleavable TEV;V5
    Expression
    Mammalian
    Mutation
    H. sapiens recode 1
    Promoter
    CMV
    Available since
    July 30, 2020
    Availability
    Industry, Academic Institutions, and Nonprofits
    154795-plasmid-map-sequence-id-311546

    Enlarge

  17. pGBW-m4252845

    Plasmid
    #153787
    Purpose
    Mammalian expression plasmid for SARS-CoV-2 S (surface glycoprotein)
    Depositor
    Ginkgo Bioworks, Benjie Chen
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    SARS-CoV-2 S (surface glycoprotein) (S Severe acute respiratory syndrome coronavirus 2)
    Tags
    Cleavable TEV;6xHis-003
    Expression
    Mammalian
    Mutation
    wt
    Promoter
    CMV
    Available since
    June 12, 2020
    Availability
    Industry, Academic Institutions, and Nonprofits
    153787-plasmid-map-sequence-id-307368

    Enlarge

  18. pGBW-m4046940

    Plasmid
    #149261
    Purpose
    Bacterial expression plasmid for SARS-CoV-2 RNA-dependent RNA polymerase
    Depositor
    Ginkgo Bioworks, Benjie Chen
    Insert
    SARS-CoV-2 RNA-dependent RNA polymerase (ORF1ab Escherichia coli str. K-12 substr. MG1655; Severe acute respiratory syndrome coronavirus 2)
    Tags
    Cleavable TEV;StrepII
    Expression
    Bacterial
    Mutation
    wt
    Promoter
    Promoter | pT7 ; Operator | lacO ; RBS | T7_rbs ;…
    Available since
    May 12, 2020
    Availability
    Industry, Academic Institutions, and Nonprofits
    149261-plasmid-map-sequence-id-296392

    Enlarge

  19. pGBW-m4046901

    Plasmid
    #145725
    Purpose
    Bacterial Expression plasmid for SARS-CoV-2 ORF7a protein
    Depositor
    Ginkgo Bioworks, Benjie Chen
    Insert
    SARS-CoV-2 ORF7a protein (ORF7a Escherichia coli str. K-12 substr. MG1655; Severe acute respiratory syndrome coronavirus 2)
    Tags
    Cleavable TEV;6xHIS
    Expression
    Bacterial
    Mutation
    Escherichia coli recode 1
    Promoter
    Promoter | pT7 ; Operator | lacO ; RBS | T7_rbs ;…
    Available since
    April 29, 2020
    Availability
    Industry, Academic Institutions, and Nonprofits
    145725-plasmid-map-sequence-id-297834

    Enlarge

  20. pGBW-m4046832

    Plasmid
    #145691
    Purpose
    Bacterial Expression plasmid for SARS-CoV-2 nsp7
    Depositor
    Ginkgo Bioworks, Benjie Chen
    Insert
    SARS-CoV-2 nsp7 (ORF1ab Escherichia coli str. K-12 substr. MG1655; Severe acute respiratory syndrome coronavirus 2)
    Tags
    Cleavable TEV;StrepII
    Expression
    Bacterial
    Mutation
    Escherichia coli recode 1
    Promoter
    Promoter | pT7 ; Operator | lacO ; RBS | T7_rbs ;…
    Available since
    April 29, 2020
    Availability
    Industry, Academic Institutions, and Nonprofits
    145691-plasmid-map-sequence-id-298369

    Enlarge

Showing: 5321 - 5340 of 14065 results