We narrowed to 150,834 results for: ins

1. PKG I beta WT

   Plasmid

   #16394

   Depositor

   Bernard Weinstein

   Article

   Deguchi et al Mol Cancer Ther. 2002 Aug . 1(10):803-9.

   Insert

   PKG1 beta (PRKG1 Human)
   

   Tags

   HA

   Expression

   Mammalian

   Available Since

   April 14, 2009

   Availability

   Academic Institutions and Nonprofits only

   

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2. SpyCatcher002-oHex

   Plasmid

   #124670

   Purpose

   Expresses SpyCatcher002-oHex for hexamerization of SpyTag-proteins via hexameric coiled-coil

   Depositor

   Mark Howarth

   Article

   Khairil Anuar et al Nat Commun. 2019 Apr 15;10(1):1734. doi:

   Insert

   SpyCatcher002-oHex
   

   Tags

   C-tag, His6, and TEV cleavage site

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   May 6, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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3. pFA6a-link-ymYPET-URA3

   Plasmid

   #168056

   Purpose

   Replaces Addgene plasmid 118460. Yeast tagging vector to create a gene fusion with yeast optimized mYPET with CaUra3 selection; Contains the linker region at the 5’ end of the FP

   Depositor

   Bas Teusink

   Article

   Botman et al Sci Rep. 2019 Feb 19;9(1):2234. doi: 10.

   Insert

   ymYPET
   

   Tags

   ymYPET

   Expression

   Yeast

   Available Since

   June 18, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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4. pcDNA3.1_Tiam1-pMag(3x)-iRFP

   Plasmid

   #67305

   Purpose

   Blue light dependent photoswitching system

   Depositor

   Moritoshi Sato

   Article

   Kawano et al Nat Commun. 2015 Feb 24;6:6256. doi: 10.

   Insert

   Tiam1-pMag(3x)-iRFP (Tiam1 Synthetic, Mouse)
   

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   July 9, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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5. PT7-deGFP-MGapt

   Plasmid

   #67741

   Purpose

   T7 promoter expressing deGFP-MG aptamer fusion

   Depositor

   Richard Murray

   Article

   Siegal-Gaskins et al ACS Synth Biol. 2014 Jun 20;3(6):416-25.

   Insert

   UTR1-deGFP-MGapt-T7term
   

   Use

   Synthetic Biology

   Expression

   Bacterial

   Promoter

   PT7

   Available Since

   Sept. 2, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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6. pTRE_tau-LacZ::tTA-H100Y

   Plasmid

   #34927

   Depositor

   Mark Mayford

   Article

   Reijmers et al Science. 2007 Aug 31;317(5842):1230-3.

   Inserts

   tau (MAPT Bovine)
   tTA
   

   Use

   Mouse Targeting

   Tags

   LacZ

   Expression

   Mammalian

   Mutation

   Made doxycycline insensitive by introduction of a…

   Promoter

   tetO

   Available Since

   Aug. 2, 2012

   Availability

   Academic Institutions and Nonprofits only

   

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7. mKOkappa-2A-mTurquoise2

   Plasmid

   #98837

   Purpose

   Produces equimolar levels of two fluorescent proteins. It can be used to measure practical brightness and as a negative control for FRET

   Depositor

   Dorus Gadella

   Article

   Mastop et al Sci Rep. 2017 Sep 20;7(1):11999. doi: 10

   Insert

   mKOkappa-2A-mTurquoise2
   

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Aug. 17, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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8. pLVX-RT006-GCN4-HaloPM

   Plasmid

   #163386

   Purpose

   To express 4X sGCN4 module in the G-baToN system

   Depositor

   Monte Winslow

   Article

   Tang et al Elife. 2020 Oct 7;9. pii: 61080. doi: 10

   Insert

   4XGCN4-Halotag-PDGFRtm-2A-mCherry (GCN4-HaloPM)
   

   Use

   Lentiviral

   Tags

   mCherry

   Expression

   Bacterial and Mammalian

   Promoter

   EF1a

   Available Since

   Feb. 8, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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9. pDOC-K-glmS-GFPfor

   Plasmid

   #158059

   Purpose

   Donor plasmid for insertion of eGFP and the constitutive acpP promoter into the S. Typhimurium chromosome. GFP is in the forward orientation relative to the kanamycin resistance selectable marker

   Depositor

   Mark Webber

   Article

   Holden et al Microbiology (Reading). 2020 Nov 23. doi

   Insert

   Green Fluorescent Protein optimised for excitation with UV light
   

   Use

   Synthetic Biology

   Expression

   Bacterial

   Promoter

   acpP

   Available Since

   Sept. 15, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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10. SpyCatcher002-oPent

    Plasmid

    #124664

    Purpose

    Expresses SpyCatcher002-oPent for pentamerization of SpyTag-proteins via pentameric coiled-coil

    Depositor

    Mark Howarth

    Article

    Khairil Anuar et al Nat Commun. 2019 Apr 15;10(1):1734. doi:

    Insert

    SpyCatcher002-oPent
    

    Tags

    C-tag, His6, and TEV cleavage site

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    May 6, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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11. pFastBac1 PIK3R1

    Plasmid

    #17743

    Depositor

    Bert Vogelstein

    Article

    Samuels et al Cancer Cell. 2005 Jun . 7(6):561-73.

    Insert

    PIK3R1 (PIK3R1 Human)
    

    Expression

    Insect

    Available Since

    April 14, 2008

    Availability

    Academic Institutions and Nonprofits only

12. pAct:dCas9-GCN4

    Plasmid

    #78899

    Purpose

    Expresses dCas9-GCN4 under actin promoter for SunTag CRISPRa in Drosophila cell culture

    Depositor

    Norbert Perrimon

    Article

    Chavez et al Nat Methods. 2016 May 23. doi: 10.1038/n

    Insert

    dCas9-10XGCN4
    

    Use

    CRISPR

    Tags

    HA

    Expression

    Insect

    Promoter

    pActin (Drosophila)

    Available Since

    Aug. 12, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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13. pT2ADWpuro_2paRAF

    Plasmid

    #129653

    Purpose

    For generating cell line expressing the 2P-activatable RAF system with P2A and full-length RAF1

    Depositor

    Michiyuki Matsuda

    Article

    Kinjo et al Nat Methods. 2019 Sep 9. pii: 10.1038/s4

    Insert

    2paCRY2-Raf1-2A-CIBN-mScarlet-KRasCAAX
    

    Expression

    Mammalian

    Promoter

    CAG

    Available Since

    Sept. 12, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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14. pcDNA3-K-I-N-CAAX

    Plasmid

    #41640

    Purpose

    Expresses caged Cdc42 GEF intersectin: Intersectin Dbl homology (DH) domain fused to wild-type Dronpa145K at C terminus and Dronpa145N at N terminus, for optogenetic activation

    Depositor

    Michael Lin

    Article

    Zhou et al Science. 2012 Nov 9;338(6108):810-4. doi

    Insert

    Dronpa145K-IntersectinDH-Dronpa145N-CAAX
    

    Tags

    CAAX box

    Expression

    Mammalian

    Mutation

    K145N on Dronpa

    Promoter

    CMV

    Available Since

    Dec. 21, 2012

    Availability

    Academic Institutions and Nonprofits only

    

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15. pLenti X2 Zeo DEST (749-3)

    Plasmid

    #21562

    Purpose

    3rd gen lentiviral Gateway destination vector, RNAi, 3'LTR insertion, Zeo

    Depositor

    Eric Campeau

    Article

    Campeau et al PLoS One. 2009 Aug 6;4(8):e6529.

    Type

    Empty backbone

    Use

    Lentiviral and RNAi

    Expression

    Mammalian

    Available Since

    July 22, 2009

    Availability

    Academic Institutions and Nonprofits only

    

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16. pAAV-rTH-tTA

    Plasmid

    #133268

    Purpose

    An AAV genome that expresses tTA from the rat TH promoter

    Depositor

    Viviana Gradinaru

    Article

    Robinson et al Elife. 2019 Sep 23;8. pii: 48983. doi: 1

    Insert

    tTA
    

    Use

    AAV

    Expression

    Mammalian

    Mutation

    *(see below)

    Promoter

    rTH

    Available Since

    Nov. 11, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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17. pAct:dCas9

    Plasmid

    #78903

    Purpose

    Expresses dCas9 under actin promoter for "Scaffold" CRISPRa in Drosophila cells

    Depositor

    Norbert Perrimon

    Article

    Chavez et al Nat Methods. 2016 May 23. doi: 10.1038/n

    Insert

    dCas9
    

    Use

    CRISPR

    Expression

    Insect

    Promoter

    pActin (Drosophila)

    Available Since

    Aug. 12, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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18. SpyCatcher002-oHept

    Plasmid

    #124671

    Purpose

    Expresses SpyCatcher002-oHept for heptamerization of SpyTag-proteins via heptameric coiled-coil

    Depositor

    Mark Howarth

    Article

    Khairil Anuar et al Nat Commun. 2019 Apr 15;10(1):1734. doi:

    Insert

    SpyCatcher002-oHept
    

    Tags

    C-tag, His6, and TEV cleavage site

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    May 6, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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19. pKLBac-FluB-trimer

    Plasmid

    #229859

    Purpose

    Influenza B/Memphis/13/03 polymerase heterotrimer (self-cleaving polyprotein)

    Depositor

    Stephen Cusack

    Article

    Reich et al Nature. 2014 Dec 18;516(7531):361-6. doi

    Inserts

    PA, Influenza B (B/Memphis/13/03)
    PB1, Influenza B (B/Memphis/13/03)
    PB2, Influenza B (B/Los Angeles/1/02)
    

    Tags

    2x-Streptag and 8xHis-tag

    Expression

    Insect

    Available Since

    Feb. 25, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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20. pCOLA banana sensor sfGFP

    Plasmid

    #107367

    Purpose

    Expresses sfGFP (a GFP) in Ecoli off a T7 promoter when exposed to extracted and RPA-amplified banana DNA

    Depositor

    James Collins

    Article

    Huang et al Sci Adv. 2018 Aug 1;4(8):eaat5105. doi:

    Insert

    banana-detecting toehold + sfGFP
    

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Oct. 3, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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