We narrowed to 152,219 results for: ins
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Plasmid#112685PurposeExpress hSpCas9 under nanos promoterDepositorInserthSpCas9
Tags3x FLAG, EGFP, and NLSExpressionInsectMutationHumanized Cas9 bacterial sequencePromoternanosAvailable SinceJan. 8, 2019AvailabilityAcademic Institutions and Nonprofits only -
PKG I alpha WT
Plasmid#16390DepositorAvailable SinceApril 14, 2009AvailabilityAcademic Institutions and Nonprofits only -
pFA6a-link-ymTq2-URA3
Plasmid#168057PurposeReplaces Addgene Plasmid 118458. Yeast tagging vector to create a gene fusion with yeast optimized mTurquoise2 with CaUra3 selection; Contains the linker region at the 5’ end of the FPDepositorInsertymTurquoise2
TagsymTurquoise2ExpressionYeastAvailable SinceJune 18, 2021AvailabilityAcademic Institutions and Nonprofits only -
AICSDP-166:CDH1-mEGFP
Plasmid#193921PurposeHomology arms and LE-mEGFP sequence for C-terminus tagging of human cadherin 1DepositorInsertcadherin 1 Homology Arms with LE-mEGFP (CDH1 Human)
UseAAV and CRISPR; Donor templateTagslinker-mEGFPMutationhomology arms contain point mutations to disrupt …Available SinceDec. 22, 2022AvailabilityIndustry, Academic Institutions, and Nonprofits -
AAV-DeltaJunD
Plasmid#68547PurposeAAV expression of DeltaJunDDepositorInsertDeltaJunD-IRES-GFP
UseAAVExpressionMammalianMutationtruncated splice variantPromoterCMVAvailable SinceJuly 11, 2018AvailabilityAcademic Institutions and Nonprofits only -
pLVX-RT005-HaloGPM
Plasmid#163385Purpose2nd generation lentiviral vector to express sHalotag-sGFP module in the G-baToN systemDepositorInsertHalotag-GFP-PDGFRtm-2A-mCherry (HaloGPM)
UseLentiviralTagsmCherryExpressionBacterial and MammalianPromoterEF1aAvailable SinceJan. 11, 2021AvailabilityAcademic Institutions and Nonprofits only -
AICSDP-079:DCP1A-mEGFP
Plasmid#193922PurposeHomology arms and mEGFP-SRA for N-terminus tagging of human DCP1ADepositorInsertDCP1A Homology Arms with mEGFP-SRA (DCP1A Human)
UseCRISPR; Donor templateTagsmEGFP-linkerMutationhomology arms contain point mutations to disrupt …Available SinceDec. 22, 2022AvailabilityIndustry, Academic Institutions, and Nonprofits -
pFUGW-hGtACR1-EYFP
Plasmid#67795PurposeMammalian expression construct of human codon-adapted anion channelrhodopsin 1 from Guillardia theta fused in frame to EYFP via a NotI site in the pFUGW vector backboneDepositorInserthGtACR1
UseLentiviralTagsEYFPExpressionMammalianAvailable SinceAug. 6, 2015AvailabilityAcademic Institutions and Nonprofits only -
p-attB-FL.hsp70P-Dam[4-HT-intein@L127C]Myc[open]
Plasmid#71805PurposeGenerating transgenic flies for 4-hydroxytamoxifen-inducible DamIDDepositorInsertFL.hsp70P-Dam[4-HT-intein@L127C]-Myc[open]
ExpressionInsectAvailable SinceMarch 2, 2016AvailabilityAcademic Institutions and Nonprofits only -
AICSDP-80:UBTF-HaloTag
Plasmid#133963PurposeHomology arms and HaloTag-linker sequence for N-terminus tagging of human UBTFDepositorInsertUBTF Homology Arms with HaloTag-linker (UBTF Human)
UseCRISPRTagsHaloTag-linkerExpressionMammalianMutationhomology arms contain point mutations to disrupt …Available SinceNov. 22, 2019AvailabilityAcademic Institutions and Nonprofits only -
pRXG
Plasmid#113643PurposeThis construct contains an amber stop codon within the linker between the two genes. The read through rate of the amber stop codon can be determined using the ratio of GFP signal to RFP signal.DepositorInsertmRFP1-linker-sfGFP
UseSynthetic BiologyAvailable SinceJuly 27, 2018AvailabilityAcademic Institutions and Nonprofits only -
pTK14
Plasmid#37360DepositorAvailable SinceJuly 19, 2012AvailabilityAcademic Institutions and Nonprofits only -
pTM1_VP35_ZEBOV
Plasmid#68121PurposeEncodes for Ebola Virus Zaire VP35 under control of a T7 RNA polymerase promoter.DepositorAvailable SinceNov. 30, 2015AvailabilityAcademic Institutions and Nonprofits only -
pSEM253 - sgRNA mosTI hygroR
Plasmid#159827PurposeSingle copy or array insertion by MosTI using split hygroR selectionDepositorInsertsgRNA mosTI hygroR
ExpressionWormMutationNot applicableAvailable SinceDec. 17, 2020AvailabilityAcademic Institutions and Nonprofits only -
pRSETb-rsGreen0.7
Plasmid#78176PurposeExpresses rsGreen0.7 in E. coli strains.DepositorInsertrsGreen0.7
Tags6xHis-tag; Xpress tagExpressionBacterialPromoterT7Available SinceMay 27, 2016AvailabilityAcademic Institutions and Nonprofits only -
pSEM270 - MCS mosTI hygroR target
Plasmid#159826PurposeSingle copy or array insertion by MosTI using split hygroR selectionDepositorInsertMCS mosTI hygroR target
ExpressionWormMutationNot applicableAvailable SinceDec. 17, 2020AvailabilityAcademic Institutions and Nonprofits only -
pBLADE_ONLY_C
Plasmid#168050PurposeExpresses the synthetic gene BLADE FP6 under the constitutive J23101** promoter.DepositorInsertBlue light-inducible AraC dimers in Escherichia coli
ExpressionBacterialPromoterJ23101**Available SinceMay 11, 2021AvailabilityAcademic Institutions and Nonprofits only -
pcDNA-NBid-PhoCl2c-CBid
Plasmid#164051PurposeOptogenetic manipulation of cell apoptosis using PhoCl2cDepositorInsertNBid-PhoCl2c-CBid
Tagskozak sequenceExpressionMammalianPromoterCMV promotorAvailable SinceFeb. 19, 2021AvailabilityAcademic Institutions and Nonprofits only -
pSEM321 - array co-injection fragment mosTI hygroR
Plasmid#159828PurposeSingle copy or array insertion by MosTI using split hygroR selectionDepositorInsertarray co-injection fragment mosTI hygroR
ExpressionWormMutationNot applicableAvailable SinceDec. 17, 2020AvailabilityAcademic Institutions and Nonprofits only -
pTM1_VP30_ZEBOV
Plasmid#69119PurposeEncodes for Ebola Virus Zaire VP30 under control of a T7 RNA polymerase promoterDepositorAvailable SinceNov. 30, 2015AvailabilityAcademic Institutions and Nonprofits only