We narrowed to 14,358 results for: cas9

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pJA54

Plasmid

#60212

Purpose

cleavage near e1350 locus in sqt-1

Depositor

Andrew Fire

Article

Arribere et al Genetics. 2014 Aug 26. pii: genetics.114

Insert

sqt-1(e1350) gRNA1

Use

CRISPR

Promoter

U6

Available Since

Oct. 2, 2014

Availability

Academic Institutions and Nonprofits only

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pSCBE3-HF

Plasmid

#133967

Purpose

Expresses HF-BE3 CRISPR base editor and gRNA scaffold in Streptomyces

Depositor

Yuhui Sun

Article

Zhong et al bioRxiv 630137

Insert

HF-BE3

Use

CRISPR and Synthetic Biology

Available Since

Dec. 16, 2019

Availability

Academic Institutions and Nonprofits only

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lentiGuide-Puro-Chen-tracrRNA_2xBsmBI

Plasmid

#196710

Purpose

Backbone for cloning single guides (Chen tracrRNA PMID: 24360272) or guide libraries with untranscribed barcodes and a tracrRNA of choice

Depositor

Bernhard Schmierer

Article

Schmierer et al Mol Syst Biol. 2017 Oct 9;13(10):945. do

Type

Empty backbone

Use

CRISPR and Lentiviral

Available Since

Sept. 28, 2023

Availability

Academic Institutions and Nonprofits only

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pJA55

Plasmid

#59935

Purpose

gRNA for cleavage near sqt-1(e1350) locus in C elegans

Depositor

Andrew Fire

Article

Arribere et al Genetics. 2014 Aug 26. pii: genetics.114

Insert

sqt-1(e1350) gRNA2

Use

CRISPR

Promoter

U6

Available Since

Sept. 22, 2014

Availability

Academic Institutions and Nonprofits only

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AP588-1

Plasmid

#71278

Purpose

Expresses myc::TEV::mTagGFP::TEV::3Xflag in bacteria

Depositor

Geraldine Seydoux

Article

Paix et al Genetics. 2015 Sep;201(1):47-54. doi: 10

Insert

tagGFP

Tags

TEV::3xFlag and myc::TEV

Expression

Bacterial

Available Since

Dec. 21, 2015

Availability

Academic Institutions and Nonprofits only

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pU6-pegRNA-EMX1+38

Plasmid

#140579

Purpose

Expresses a pegRNA in mammalian cells

Depositor

Feng Zhang

Article

Schmid-Burgk et al Mol Cell. 2020 Mar 17. pii: S1097-2765(2

Insert

pegRNA EMX1 +38

Available Since

May 21, 2020

Availability

Academic Institutions and Nonprofits only

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reporter-gT2

Plasmid

#47321

Purpose

TF reporter for gRNA-AAVS1_T2 (RNA-guided dTomato expression)

Depositor

George Church

Article

Mali et al Nat Biotechnol. 2013 Aug 1. doi: 10.1038

Insert

minCMV-dTomato_pA

Use

CRISPR

Available Since

Aug. 16, 2013

Availability

Academic Institutions and Nonprofits only
pCAG-TetR-Rad18UBD

Plasmid

#119010

Purpose

Mammalian expression of a fusion protein of single chain Tet repressor with the human Rad18 Ubiquitin binding domain and BFP expression

Depositor

Ralf Kuehn

Article

Bashir et al BMC Biotechnol. 2020 Oct 23;20(1):57. do

Insert

TetR

Expression

Mammalian

Promoter

CAG

Available Since

Oct. 28, 2020

Availability

Academic Institutions and Nonprofits only

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pJA59

Plasmid

#59932

Purpose

gRNA for cleavage at unc-109(n499) locus in C elegans

Depositor

Andrew Fire

Article

Arribere et al Genetics. 2014 Aug 26. pii: genetics.114

Insert

unc-109(n499) gRNA

Use

CRISPR

Promoter

U6

Available Since

Sept. 22, 2014

Availability

Academic Institutions and Nonprofits only

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pmCherry-U6-VEGFA

Plasmid

#140588

Purpose

Expresses a gRNA and mCherry in mammalian cells

Depositor

Feng Zhang

Article

Schmid-Burgk et al Mol Cell. 2020 Mar 17. pii: S1097-2765(2

Insert

gRNA VEGFA

Use

CRISPR

Available Since

May 21, 2020

Availability

Academic Institutions and Nonprofits only

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pU6-pegRNA-DNMT1+38

Plasmid

#140578

Purpose

Expresses a pegRNA in mammalian cells

Depositor

Feng Zhang

Article

Schmid-Burgk et al Mol Cell. 2020 Mar 17. pii: S1097-2765(2

Insert

pegRNA DNMT1 +38

Available Since

May 21, 2020

Availability

Academic Institutions and Nonprofits only

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pCAG-Trex2-bpA

Plasmid

#86984

Purpose

Expression of mouse Trex2

Depositor

Ralf Kuehn

Article

Yumlu et al Methods Mol Biol. 2019;1961:137-151. doi

Insert

mouse Trex2 coding region (Trex2 Mouse)

Expression

Mammalian

Promoter

CAG

Available Since

April 3, 2017

Availability

Academic Institutions and Nonprofits only

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pCAS_gpyrG1

Plasmid

#90276

Purpose

(also pMST621-BB3_gpyrG1_cas9) CRISPR/Cas9 plasmid with gRNA for pyrG1, Cas9

Depositor

Michael Sauer

Article

Sarkari et al Bioresour Technol. 2017 May 4. pii: S096

Insert

gRNA (pyrg1)

Use

A. niger

Expression

Bacterial

Available Since

July 19, 2017

Availability

Academic Institutions and Nonprofits only

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pMLS234

Plasmid

#73682

Purpose

SapTrap 3-site Destination vector for N-terminal GFP tagging with embedded Cbr-unc-119 selectable marker

Depositor

Erik Jorgensen

Article

Schwartz et al Genetics. 2016 Feb 2. pii: genetics.115.

Insert

GFP + Cbr-unc-119

Use

CRISPR and Cre/Lox

Tags

GFP

Expression

Worm

Available Since

April 20, 2018

Availability

Academic Institutions and Nonprofits only

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pmCherry-U6-CACNG3

Plasmid

#140590

Purpose

Expresses a gRNA and mCherry in mammalian cells

Depositor

Feng Zhang

Article

Schmid-Burgk et al Mol Cell. 2020 Mar 17. pii: S1097-2765(2

Insert

gRNA CACNG3

Use

CRISPR

Available Since

May 21, 2020

Availability

Academic Institutions and Nonprofits only

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PM-TD!TB

Plasmid

#48656

Purpose

Bacterial TD crRNA expression: targets TD to protospacer B (ACTTTAAAAGTATTCGCCAT), p15A/chloramphenicol

Depositor

George Church

Article

Esvelt et al Nat Methods. 2013 Sep 29. doi: 10.1038/n

Insert

Bacterial TD crRNA to prototspacer B

Use

CRISPR

Promoter

J23100 promoter

Available Since

Oct. 17, 2013

Availability

Academic Institutions and Nonprofits only

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pHu6-gRNA-NT1

Plasmid

#81203

Purpose

hU6 expression of gRNA NT1

Depositor

David Liu

Article

Chaikind et al Nucleic Acids Res. 2016 Aug 11. pii: gkw

Insert

hU6 expression of gRNA NT1

Expression

Mammalian

Promoter

U6

Available Since

Nov. 4, 2016

Availability

Academic Institutions and Nonprofits only

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pGEM-gate

Plasmid

#113758

Purpose

Gateway destination vector only containing the Gateway cassette and pGEM-T Easy backbone. This can be used with Gateway-compatible Homology-Directed Repair.

Depositor

Magdalena Bezanilla

Article

Mallett et al Plant Direct. 2019 Sep 12;3(9):e00168. d

Insert

gateway cassette

Use

Gateway destination vector

Available Since

Sept. 24, 2018

Availability

Academic Institutions and Nonprofits only

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pmCherry-U6-ALDH1A3

Plasmid

#140589

Purpose

Expresses a gRNA and mCherry in mammalian cells

Depositor

Feng Zhang

Article

Schmid-Burgk et al Mol Cell. 2020 Mar 17. pii: S1097-2765(2

Insert

gRNA ALDH1A3

Use

CRISPR

Available Since

May 21, 2020

Availability

Academic Institutions and Nonprofits only

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px330 EQR

Plasmid

#101733

Purpose

Expresses a sgRNA and a Cas9 EQR variant that recognizes "NGAG" PAM motifs

Depositor

Andrew Holland

Article

Lambrus et al Methods Cell Biol. 2018;144:107-135. doi

Insert

SpCas9 EQR

Tags

3xFLAG-NLS and NLS

Expression

Mammalian

Mutation

EQR (D1135E, R1335Q, and T1337R mutations in Cas9)

Available Since

Oct. 17, 2017

Availability

Academic Institutions and Nonprofits only

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