We narrowed to 23,520 results for: eng
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Plasmid#135539PurposeEncodes a specific PSD-95 binder (Xph20) fused to GCaMP7fDepositorInsertXph20
TagsGCaMP7fExpressionMammalianPromoterCAGAvailable SinceApril 9, 2021AvailabilityAcademic Institutions and Nonprofits only -
pLH-sgRNA1-boxB-MS2
Plasmid#75396PurposesgRNA1-boxB-MS2DepositorInsertsgRNA1-boxB-MS2
UseCRISPR and LentiviralTagsnoExpressionMammalianPromoterU6Available SinceMay 25, 2016AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1-CD81-(TEV)-FKBP-SmBiT
Plasmid#182651Purposehuman CD81-TEV cleavage site-FKBP-SmBiT tagDepositorInserthuman CD81-TEVp cleavage site-FKBP-SmBiT (CD81 Human, Synthetic)
TagsSmBiTExpressionMammalianPromoterCMVAvailable SinceMarch 25, 2022AvailabilityAcademic Institutions and Nonprofits only -
MBP-TEVcs(ENLYFQ/G)-His6-TEVΔ(220-242)-R5
Plasmid#135454PurposeBacterial expression of TEVΔ proteaseDepositorInsertMBP-TEVcs(ENLYFQ/G)-His6-TEVΔ(220-242)-R5
TagsMBP, His6ExpressionBacterialMutationS219V mutation improves stability. Truncated form…Promotertac promoterAvailable SinceJan. 14, 2020AvailabilityAcademic Institutions and Nonprofits only -
pIRESh-GFPII-S-Dlk1
Plasmid#108931PurposeGFP-tagged vector expressing secreted isoform of Dlk1DepositorInsertDlk1 (Dlk1 Mouse)
TagsGFPIIExpressionMammalianMutationcontains sequences from full length Dlk1A up to t…Available SinceMay 6, 2019AvailabilityAcademic Institutions and Nonprofits only -
pCMV-PE2-VRQR
Plasmid#159983PurposePrime editing in mammalian cellsDepositorInsertPE2-VRQR
TagsBpSV40 NLSExpressionMammalianPromoterCMVAvailable SinceNov. 12, 2020AvailabilityAcademic Institutions and Nonprofits only -
araC-pBAD-gusA-pBAV1k
Plasmid#30502DepositorAvailable SinceOct. 5, 2011AvailabilityAcademic Institutions and Nonprofits only -
AAV2 for Split-PE, gRNAs + MMLV-RT(dRH)-P2A-eGFP (PKC1002)
Plasmid#190112PurposeAAV2 for expression of pegRNA and ngRNA (HEKs3, CTT insertion) + MMLV-RT(dRH)-P2A-eGFPDepositorInsertsMMLV-RT(dRH)
U6-pegRNA(HEKs3, CTT ins)-H1-ngRNA(HEK s3)
UseAAVTagsbpNLS and bpNLS-P2A-eGFPMutationmutations from RT in PE2 and truncation of RNAse …PromoterEFS and U6, H1Available SinceSept. 26, 2022AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-sACE2v2.4(732)-8h
Plasmid#154103PurposeMammalian expression plasmid for dimeric soluble ACE2 high affinity variant 2.4 (8his tag)DepositorInsertAngiotensin-converting enzyme 2 (ACE2 Human)
TagsShort GSG linker and 8his tagExpressionMammalianMutationExtracellular, soluble protease domain (a.a. M1-G…PromoterCMVAvailable SinceJune 24, 2020AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-sACE2v1-Fc(IgG1)
Plasmid#145164PurposeMammalian expression plasmid for soluble ACE2-Fc fusion (IgG1) high affinity variant 1DepositorInsertAngiotensin-converting enzyme 2 (ACE2 Human)
TagsFc region of human IgG1ExpressionMammalianMutationExtracellular, soluble protease domain (a.a. M1-D…PromoterCMVAvailable SinceApril 29, 2020AvailabilityAcademic Institutions and Nonprofits only -
pSuperGFP-cdc42-prenyl siRNA
Plasmid#22715DepositorAvailable SinceFeb. 1, 2010AvailabilityAcademic Institutions and Nonprofits only -
Marathon-RT pentamutant for Split-PE (pEH107)
Plasmid#190110PurposeExpresses Marathon-RT pentamutant (D14R-N26R-D74R-N116K-N197R)DepositorInsertMarathon-RT
TagsbpNLSExpressionMammalianMutationD14R, N26R, D74R, N116K, N197RPromoterCMVAvailable SinceSept. 26, 2022AvailabilityAcademic Institutions and Nonprofits only -
YFVdelCME-mVenus
Plasmid#179953PurposepCCI vector encoding YFV-17D lacking C, prM, E proteins but with mVenusDepositorInsertYFV-17D lacking C, prM, E proteins but with mVenus
UseTransneuronal tracingPromoterSP6Available SinceFeb. 17, 2022AvailabilityAcademic Institutions and Nonprofits only -
pMS79 (eft-3p::Cas9 + sgRNA)
Plasmid#154839PurposeCas9 + sgRNA plasmid that is targeted to the synthetic guide sequence GGACAGTCCTGCCGAGGTGGDepositorInsertsgRNA
UseCRISPRExpressionWormAvailable SinceAug. 6, 2020AvailabilityAcademic Institutions and Nonprofits only -
pJZC519
Plasmid#62278PurposeYeast dCas9-VP64 expression plasmidDepositorInsertdCas9-VP64
TagsHas a VP64 fusionExpressionYeastMutationHas mutations in the RuvC1 and HNH domains to ren…PromoterpTdh3Available SinceMay 8, 2015AvailabilityAcademic Institutions and Nonprofits only -
pEF005-sgRNA-shuffle-in
Plasmid#104441PurposeProvide and shuffle a cassette of AtU6:sgRNA-transRNA into SM-destination vectors (pRW006 and pRW004) with golden gate cloning strategy. Work together with pEF004.DepositorInsertsgRNA-transRNA transcription by U6 promoter
UseCRISPRExpressionBacterial and PlantAvailable SinceJan. 10, 2018AvailabilityAcademic Institutions and Nonprofits only -
pCI Cas9 H2B-RFP
Plasmid#92397PurposeCAG-driven ubiquitous expression of Cas9 with Histone2B-RFP reporter controlled by IRESDepositorInsertCas9
UseCRISPRExpressionMammalianPromoterCAGAvailable SinceAug. 24, 2017AvailabilityAcademic Institutions and Nonprofits only -
dCas9-10xSunTag-P2A-tagBFP
Plasmid#174141PurposeExpresses the dCas9-10xSunTag-tagBFP fusion protein for the recruitment of scFv(GCN4)-fused effector domains. A shorter version of the Addgene 60903 plasmid.DepositorInsertdCas9, 10xSunTag, tagBFP
TagsHA and tagBFPExpressionMammalianPromoterSV40Available SinceOct. 22, 2021AvailabilityAcademic Institutions and Nonprofits only -
pSC-PreE2
Plasmid#52264PurposeBacterial expression of GST-tagged TARGET proteins fused to SUMO-E2 with PreScission protease cleavage site linkerDepositorAvailable SinceJuly 10, 2014AvailabilityAcademic Institutions and Nonprofits only -
AAV-HAPLN1
Plasmid#200777PurposeExpresses full-length mouse HAPLN1 fused with cysteine-free GFP (cfGFP) under synapsin promoterDepositorHas ServiceAAV8InsertHyaluronan And Proteoglycan Link Protein 1 (Hapln1 Mouse)
UseAAVTagscfGFPPromoterSynapsinAvailable SinceJune 14, 2023AvailabilityAcademic Institutions and Nonprofits only