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We narrowed to 18,241 results for: multi

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Showing: 5341 - 5360 of 18241 results

  5. pLenti spCas9 T2A iRFP670 P2A puro

    Plasmid
    #122182
    Purpose
    The plasmid codes for a Flag-spCas9 protein, a iRFP670 fluorescent protein and a puromycin resistance. The plasmid has two BsmBI acceptor sites to insert gRNA expressing sequences.
    Depositor
    Raphael Gaudin
    Inserts
    spCas9 (NEWENTRY )
    iRFP670
    Use
    Lentiviral
    Tags
    T2A
    Expression
    Mammalian
    Available since
    Feb. 28, 2019
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    2 Articles
    122182-plasmid-map-sequence-id-237289

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  6. pU6_(GLuc)_INT[Spinach2]

    Plasmid
    #68430
    Purpose
    Transient expression in mammalian cells of an "INT" construct_bearing the "Spinach2" aptamer, targeting the GLuc reporter. U6 promoter
    Depositor
    John Rinn
    Publication
    Shechner et al Nat Methods. 2015 Jul;12(7):664-70. doi:
    Insert
    INT construct_bearing the "Spinach2" aptamer
    Use
    CRISPR and Synthetic Biology
    Expression
    Mammalian
    Promoter
    human U6
    Available since
    Oct. 5, 2015
    Availability
    Academic Institutions and Nonprofits only
    68430-plasmid-map-sequence-id-120208

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  7. pAAV-Ef1a-fDIO EYFP (AAV Retrograde)

    Viral prep
    #55641-AAVrg
    Purpose
    Ready-to-use AAV Retrograde particles produced from pAAV-Ef1a-fDIO EYFP (#55641). In addition to the viral particles, you will also receive purified pAAV-Ef1a-fDIO EYFP plasmid DNA. EF1a-driven, Flp-dependent expression of EYFP. These AAV were produced with a retrograde serotype, which permits retrograde access to projection neurons. These AAV preparations are suitable purity for injection into animals.
    Depositor
    Karl Deisseroth
    Publication
    Fenno et al Nat Methods. 2014 Jul;11(7):763-72. doi:
    Promoter
    EF1a
    Tags
    EYFP (Flp-dependent)
    Available since
    Oct. 21, 2024
    Availability
    Academic Institutions and Nonprofits only

  8. pH2rU3_ForInd_sinobiological_617.2_Spiked21_CMV_ZsGT2APurR

    Plasmid
    #204151
    Purpose
    Lentivirus backbone with Delta spike under an inducible promoter and constitutive expressed zsGreen-T2A-PuR genes
    Depositor
    Jesse Bloom
    Publication
    Dadonaite et al Cell. 2023 Mar 16;186(6):1263-1278.e20.
    Insert
    SARS-CoV-2 Delta variant spike
    Use
    Lentiviral
    Expression
    Mammalian
    Mutation
    21 amino acid cytoplasmic tail deletion
    Promoter
    TRE3G
    Available since
    Aug. 16, 2023
    Availability
    Academic Institutions and Nonprofits only
    204151-plasmid-map-sequence-id-404782

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  10. pMM643

    Plasmid
    #112533
    Purpose
    pZE2-PLhrtO-lux-hrtR-RBS3-chuA - HrtR RBS variant of plasmid pMM627 (Strength 33545.5 AU), ColE1 origin, KanR
    Depositor
    Timothy Lu
    Publication
    Mimee et al Science. 2018 May 25;360(6391):915-918.
    Inserts
    HrtR
    ChuA
    luxCDABE
    Expression
    Bacterial
    Mutation
    A17P
    Promoter
    J23107, PL(HrtO), and ProD
    Available since
    Feb. 6, 2019
    Availability
    Academic Institutions and Nonprofits only
    112533-plasmid-map-sequence-id-216001

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  12. p3E-mTagBFP-CAAX

    Plasmid
    #75176
    Purpose
    Membrane targeted TagBFP (blue) fluorophore with stop codon for C-terminal fusions
    Depositor
    Nicholas Cole
    Publication
    Don et al Zebrafish. 2016 Sep 15.
    Insert
    mTagBFP-CAAX
    Use
    Three prime entry vector for multisite gateway th…
    Promoter
    n/a
    Available since
    Oct. 6, 2016
    Availability
    Academic Institutions and Nonprofits only
    75176-plasmid-map-sequence-id-154502

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  15. Tol2-CAG::Mitbow

    Plasmid
    #158994
    Purpose
    Multicolor mitochondrial-restricted labeling with a genome-integrating Tol2 transposon vector (randomly expressing mito-tethered mEYFP, mCherry or mCerulean upon Cre recombination)
    Depositor
    Jean Livet
    Publication
    Loulier et al Neuron. 2014 Feb 5;81(3):505-20. doi: 10
    Insert
    Mitbow
    Tags
    The default expressed EBFP2 is fused to H2B; oth…
    Expression
    Mammalian
    Promoter
    CAG
    Available since
    Oct. 1, 2020
    Availability
    Academic Institutions and Nonprofits only
    158994-plasmid-map-sequence-id-311800

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  18. pAAV_LP1B_St1Cas9_LMD-9_SpA_U6_sgRNA

    Plasmid
    #110624
    Purpose
    A single vector AAV-Cas9 system containing a liver-specific promoter with Cas9 from Streptococcus thermophilus CRISPR1 (St1Cas9 LMD-9) and its U6-driven sgRNA
    Depositor
    Yannick Doyon
    Publication
    Agudelo et al Genome Res. 2020 Jan;30(1):107-117. doi:
    Inserts
    St1Cas9 LMD-9
    sgRNA for St1Cas9
    Use
    AAV, CRISPR, and Mouse Targeting
    Tags
    SV40 NLS
    Expression
    Mammalian
    Promoter
    LP1B and hU6
    Available since
    May 25, 2018
    Availability
    Academic Institutions and Nonprofits only
    110624-plasmid-map-sequence-id-211314

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  19. pT7-agrBD-I

    Plasmid
    #53439
    Purpose
    T7-dependent expression of the type-I agrB and agrD genes of S. aureus RN4220
    Depositor
    Cynthia Collins
    Publication
    Marchand et al Biotechnol Bioeng. 2013 Nov;110(11):3003
    Insert
    agrBD locus
    Use
    Synthetic Biology
    Tags
    V5 epitope, 6x HIS (not expressed on agrB or agrD…
    Expression
    Bacterial
    Mutation
    none
    Promoter
    T7-lac
    Available since
    June 3, 2014
    Availability
    Academic Institutions and Nonprofits only
    53439-plasmid-map-sequence-id-83233

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Showing: 5341 - 5360 of 18241 results