We narrowed to 8,646 results for: dykddddk
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Plasmid#208381Purposelentiviral vector for expressing human B3GALT5 with C-terminal myc-DDK tag, includes nucleotide change in PAM targeting sequenceDepositorInsertbeta-1,3-galactosyltransferase 5 (B3GALT5 Human)
UseLentiviralTagsmyc, FLAGExpressionMammalianMutationnucleotide change in PAM targeting sequence nt 51…PromoterEF-1 alpha core promoterAvailable sinceSept. 3, 2024AvailabilityAcademic Institutions and Nonprofits only -
lentiMutB3GALT5catdead-blast
Plasmid#220869Purposelentiviral vector for expressing human B3GALT5 with D156A and D243A inactivating mutations and C-terminal myc-DDK tag, includes nucleotide change in PAM targeting sequenceDepositorInsertbeta-1,3-galactosyltransferase 5 (B3GALT5 Human)
UseLentiviralTagsmyc, FLAGExpressionMammalianMutationencodes D156A and D243A mutations; also, nucleoti…Available sinceSept. 3, 2024AvailabilityAcademic Institutions and Nonprofits only -
pgRNA-R-gfp
Plasmid#220927PurposeExpresses gfp using J23119 promoter and pgRNA_AT backboneDepositorInsertsuperfolder GFP
TagsFLAGExpressionBacterialPromoterJ23119Available sinceAug. 26, 2024AvailabilityAcademic Institutions and Nonprofits only -
HA-MBP-TEVpCS-ADGRC1 CTFdeltaTA-FLAG
Plasmid#217717PurposeMammalian expression of Adhesion G Protein-Coupled Receptor transmembrane domainsDepositorInsertCELSR1 (CELSR1 Human)
TagsFLAG and HAExpressionMammalianMutationDeletion of residues 1-2453Available sinceAug. 19, 2024AvailabilityAcademic Institutions and Nonprofits only -
HA-MBP-TEVpCS-ADGRB2 CTFdeltaTA-FLAG
Plasmid#217715PurposeMammalian expression of Adhesion G Protein-Coupled Receptor transmembrane domainsDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertADGRB2 (BAI2 Human)
TagsFLAG and HAExpressionMammalianMutationDeletion of residues 1-917Available sinceAug. 19, 2024AvailabilityAcademic Institutions and Nonprofits only -
pcDNA-DDX53-FLAG
Plasmid#222241PurposeExpress FLAG tagged DDX53 in mammalian cellsDepositorAvailable sinceAug. 14, 2024AvailabilityAcademic Institutions and Nonprofits only -
pTarget_AT1R-tTA
Plasmid#222851PurposeMammalian expression plasmid encoding the human Angiotensin II type I receptor fused to the tTA transcriptional activatorDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertAT1R (AGTR1 Human)
TagsFLAG and tTAExpressionMammalianAvailable sinceAug. 9, 2024AvailabilityAcademic Institutions and Nonprofits only -
pcDNA-DDX53-GFP
Plasmid#222239PurposeExpress GFP tagged DDX53 in mammalian cellsDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertDDX53 (DDX53 Human)
TagsGFPExpressionMammalianPromoterCMV and T7Available sinceAug. 5, 2024AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1+FLAG-KrascomQ61R
Plasmid#206845PurposeTo express mouse Kras encoded by common mouse codons (to increase expression) and with a Q61R mutation with an N-terminal FLAG tag.DepositorInsertKras (Kras Mouse)
TagsFLAGExpressionMammalianMutationCodons altered to most optimum based on mouse cod…Available sinceJuly 31, 2024AvailabilityAcademic Institutions and Nonprofits only -
pMXS-IRES-Blast FLVCR1b-3X FLAG
Plasmid#218536Purposehuman FLVCR1b with C-terminal 3X_FLAG cDNADepositorInsertFLVCR1 (FLVCR1 Human)
UseRetroviralTagsFLAG (3X)ExpressionMammalianMutationAlternative isoformAvailable sinceJuly 30, 2024AvailabilityAcademic Institutions and Nonprofits only -
pHIV-NAT-FLAG-CIP2A ∆NES (del561-625)
Plasmid#222625PurposeLentiviral vector that expresses Flag-tagged CIP2A NES mutant in mammalian cellsDepositorInsertCIP2A (CIP2A Human)
UseLentiviralTagsFlagExpressionMammalianMutationDeletion of the residues 561-625 (ΔNES)PromoterEF-1-alpha promoterAvailable sinceJuly 18, 2024AvailabilityIndustry, Academic Institutions, and Nonprofits -
pRetroX-TRE3G-YopE
Plasmid#221328PurposeDox-inducible expression of YopE in mammalian cells by retroviral transductionDepositorInsertYopE
UseRetroviralTagsFLAGPromoterTRE3GAvailable sinceJuly 16, 2024AvailabilityAcademic Institutions and Nonprofits only -
pMXS-IRES-BLAST flag-CAD S1406A
Plasmid#169820PurposeExpresses C-terminal flag-tagged CAD with mutation of reported PKA phosphorylation siteDepositorInsertCAD (CAD Human)
UseRetroviralTagsFlag tagExpressionMammalianMutationS1406A; TCCC -> AGTC silent mutations at nt527…Available sinceJuly 15, 2024AvailabilityAcademic Institutions and Nonprofits only -
pMXS-IRES-BLAST flag-CAD S1859A
Plasmid#169821PurposeExpresses C-terminal flag-tagged CAD with mutation of reported S6K phosphorylation siteDepositorInsertCAD (CAD Human)
UseRetroviralTagsFlag tagExpressionMammalianMutationS1859A; TCCC -> AGTC silent mutations at nt527…Available sinceJuly 15, 2024AvailabilityAcademic Institutions and Nonprofits only -
pMSCV-MAVS(IRSp53 pLxIS)
Plasmid#221315PurposeExpression of MAVS chimera with IRSp53 pLxIS domain in mammalian cells by retroviral transductionDepositorAvailable sinceJuly 10, 2024AvailabilityAcademic Institutions and Nonprofits only -
pLenti-opCas12a-Ultra-6xNLS
Plasmid#209024PurposeLentiviral vector expressing human codon-optimized opCas12a-Ultra with 6xNLSDepositorInsertopCas12a-Ultra-6xNLS-Flag-Myc-NeoR
UseCRISPR and LentiviralTagsFLAG and MYCExpressionMammalianMutationE174R/M537R/S542R/F870LPromoterEF-1aAvailable sinceJune 20, 2024AvailabilityAcademic Institutions and Nonprofits only -
pHIV-EGFP.Flag-EZH2-mt2*
Plasmid#220246PurposeExpresses an EZH2 mutant for knockout/rescue experiments in mammalian cells.DepositorInsertEZH2 mt 2* (EZH2 Human)
UseLentiviralTagsFlagExpressionMammalianMutationPRKKKR494-499NAAIRSPromoterEF-1αAvailable sinceJune 12, 2024AvailabilityAcademic Institutions and Nonprofits only -
pHIV-EGFP.Flag-EZH2-mt1
Plasmid#220244PurposeExpresses an EZH2 mutant for knockout/rescue experiments in mammalian cells.DepositorInsertEZH2 mt 1 (EZH2 Human)
UseLentiviralTagsFlagExpressionMammalianMutationR27A, R31A, F165APromoterEF-1αAvailable sinceJune 12, 2024AvailabilityAcademic Institutions and Nonprofits only -
pMXS-IRES-Blast FLVCR1/FLVCR1a-3X FLAG
Plasmid#218534Purposehuman FLVCR1/FLVCR1a with C-terminal 3x-FLAG cDNADepositorAvailable sinceJune 12, 2024AvailabilityAcademic Institutions and Nonprofits only -
MRGPRX4-DuET
Plasmid#213347PurposeThe DuET construct encodes the named human GPCR engineered with a cleaved hemagglutinin signal peptide, a 5' FLAG and a 3' 1D4 epitope tag optimized for expression in mammalian cells and proteomics studies.DepositorAvailable sinceApril 30, 2024AvailabilityAcademic Institutions and Nonprofits only