Skip to main content
Addgene
Open Menu

Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

Information

In Category

Filter By

We narrowed to 5,403 results for: puc

Clear keywords
Showing: 5361 - 5380 of 5403 results

  4. pPSU1

    Plasmid
    #89439
    Purpose
    High copy number plasmid 1 to prepare Penn State DNA molecular weight ladders
    Depositor
    Song Tan
    Article
    Henrici et al Sci Rep. 2017 May 26;7(1):2438. doi: 10.
    Inserts
    500 bp EcoRV fragment
    1000 bp EcoRV fragment
    1500 bp EcoRV fragment
    2000 bp EcoRV fragment
    500 bp PstI fragment
    700 bp PstI fragment
    800 bp PstI fragment (EHMT2 Human)
    900 bp PstI fragment
    1000 bp PstI fragment (BMI1 Human)
    2000 bp PstI fragment
    Expression
    Bacterial
    Available Since
    April 28, 2017
    Availability
    Academic Institutions and Nonprofits only
    89439-plasmid-map-sequence-id-190157

    Enlarge

  6. CLYBL-(Ef1a-SBP-LNGFR-T2A-mApple)-(CAG-rtTA)-(TRE-hNIL))

    Plasmid
    #105842
    Purpose
    Donor construct for introduction of hNIL factors and SBP-LNGFR for streptavidin bead enrichment
    Depositor
    Michael Ward
    Article
    Fernandopulle et al Curr Protoc Cell Biol. 2018 Jun;79(1):e5
    Inserts
    Neurogenin 2 (NEUROG2 Human)
    ISL LIM Homeobox 1 (ISL1 Human)
    LIM Homeobox 3 (LHX3 Human)
    Optimized mApple
    rtTA3G
    SBP Delta-LNGFR
    Use
    CRISPR and TALEN
    Promoter
    CAG, EF-1alpha, and TRE3G
    Available Since
    April 13, 2018
    Availability
    Academic Institutions and Nonprofits only
    105842-plasmid-map-sequence-id-393690

    Enlarge

  7. C-term AAV-SpCas9-VRQR-gRNA_entry (HES1477)

    Plasmid
    #242660
    Purpose
    CBh promoter expression plasmid for C-terminal intein-split AAV construct with C-term of SpCas9-VRQR and pU6 SpCas9 gRNA entry cassette
    Depositor
    Benjamin Kleinstiver
    Article
    Alves et al Nat Biomed Eng. 2025 Sep 11:10.1038/s415
    Insert
    pAAV-pCbh-BPNLS-NpuC-SpCas9-VRQR-BPNLS-WPRE-bGH_PA-SpCas9_gRNA_entrycassette
    Use
    AAV and CRISPR
    Tags
    BPNLS
    Mutation
    VRQR mutations in SpCas9(S55R/D1135V/G1218R/R1335…
    Promoter
    CBh
    Available Since
    Oct. 9, 2025
    Availability
    Academic Institutions and Nonprofits only
    242660-plasmid-map-sequence-id-489092

    Enlarge

  16. C-term AAV-eVRQR-ACTA2-gRNA-A4 (mouse) (LLH310)

    Plasmid
    #242662
    Purpose
    CBh promoter expression plasmid for C-terminal intein-split AAV construct with C-term of SpCas9-VRQR and mouse gRNA-A4
    Depositor
    Benjamin Kleinstiver
    Article
    Alves et al Nat Biomed Eng. 2025 Sep 11:10.1038/s415
    Insert
    pAAV-pCbh-BPNLS-NpuC-SpCas9-VRQR-BPNLS-WPRE-bGH_PA-ACTA2_mouse_NGA_gRNA-pU6
    Use
    AAV and CRISPR
    Tags
    BPNLS
    Mutation
    VRQR mutations in SpCas9(S55R/D1135V/G1218R/R1335…
    Promoter
    CBh
    Available Since
    Oct. 9, 2025
    Availability
    Academic Institutions and Nonprofits only
    242662-plasmid-map-sequence-id-489091

    Enlarge

  17. C-term AAV-eVRQR-ACTA2-gRNA-A4 (human) (LLH301)

    Plasmid
    #242661
    Purpose
    CBh promoter expression plasmid for C-terminal intein-split AAV construct with C-term of SpCas9-VRQR and human gRNA-A4
    Depositor
    Benjamin Kleinstiver
    Article
    Alves et al Nat Biomed Eng. 2025 Sep 11:10.1038/s415
    Insert
    pAAV-pCbh-BPNLS-NpuC-SpCas9-VRQR-BPNLS-WPRE-bGH_PA-ACTA2_human_NGA_gRNA-pU6
    Use
    AAV and CRISPR
    Tags
    BPNLS
    Mutation
    VRQR mutations in SpCas9(S55R/D1135V/G1218R/R1335…
    Promoter
    CBh
    Available Since
    Oct. 9, 2025
    Availability
    Academic Institutions and Nonprofits only
    242661-plasmid-map-sequence-id-489090

    Enlarge

  19. MIP 247 CoMiP 4in1 with shRNA p53

    Plasmid
    #63726
    Purpose
    To express Oct4, Klf4, Sox2, c-Myc and hairpin RNA p53. A single plasmid reprogramming system using a mini-intronic plasmid (MIP).
    Depositor
    Mark Kay, Joseph Wu
    Article
    Diecke et al Sci Rep. 2015 Jan 28;5:8081. doi: 10.103
    Insert
    Mini-intronic-plasmid intron (POU5F1, KLF4, SOX2, MYC, TP53 Human)
    Tags
    IRES-dtomato
    Expression
    Mammalian
    Mutation
    codon-optimised
    Promoter
    SFFV for Pct4, Klf4, Sox2 and c-myc; U6 for p53
    Available Since
    Aug. 28, 2015
    Availability
    Academic Institutions and Nonprofits only
    63726-plasmid-map-sequence-id-438433

    Enlarge

Showing: 5361 - 5380 of 5403 results