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Plasmid#114092PurposeCAG promoter expression plasmid for human codon optimized AsCas12a-RVR(S542R/K548V/N552R) nuclease with C-terminal NLS and HA tagDepositorInserthuman codon optimized AsCas12a (S542R/K548V/N552R)
UseTagsNLS(nucleoplasmin) and 3xHAExpressionMammalianMutationS542R, K548V and N552RPromoterAvailable sinceDec. 11, 2018AvailabilityAcademic Institutions and Nonprofits only -
PB-CAG-NLS-dCas9-HA-2XNLS-10xGCN4-NLS-P2A-scFv-NLS-P65-HSF1-Flag-WPRE-PolyA-PGK-EM7-NeoR-polyA
Plasmid#168290PurposeCRISPR-activator Suntag-P65-HSF1 (SPH)DepositorInsertdCas9, Suntag-P65-HSF1
UseTagsExpressionMammalianMutationPromoterCAGAvailable sinceMay 5, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCAG-hAsCas12a-enRR(E174R/S542R/K607R)-NLS(nucleoplasmin)-3xHA (AAS1077)
Plasmid#114095PurposeCAG promoter expression plasmid for human codon optimized AsCas12a-enRR(E174R/S542R/K607R) nuclease with C-terminal NLS and HA tagDepositorInserthuman codon optimized AsCas12a-RR (E174R/S542R/K607R)
UseTagsNLS(nucleoplasmin) and 3xHAExpressionMammalianMutationE174R, S542R and K607RPromoterAvailable sinceDec. 11, 2018AvailabilityAcademic Institutions and Nonprofits only -
pCAG-hAsCas12a-enRVR(E174R/S542R/K548V/N552R)-NLS(nucleoplasmin)-3xHA (AAS1060)
Plasmid#114093PurposeCAG promoter expression plasmid for human codon optimized AsCas12a-enRVR(E174R/S542R/K548V/N552R) nuclease with C-terminal NLS and HA tagDepositorInserthuman codon optimized AsCas12a-enRVR (E174R/S542R/K548V/N552R)
UseTagsNLS(nucleoplasmin) and 3xHAExpressionMammalianMutationE174R, S542R, K548V and N552RPromoterAvailable sinceDec. 11, 2018AvailabilityAcademic Institutions and Nonprofits only -
pCAG-NLS(SV40)x2-rAPOBEC1-gs-XTEN-gs-hdLbCas12a(D832A)-gs-UGI-NLS(SV40)(LbBE1.4) (RTW1293)
Plasmid#114086PurposeCAG promoter expression plasmid for human codon optimized LbBE1.4DepositorInserthuman codon optimized DNase-inactive (D832A) LbCas12a fused to N-terminal rAPOBEC1 and C-terminal UGI (LbBE1.4)
UseTags2x SV40 NLS and SV40 NLSExpressionMammalianMutationD832APromoterCAGAvailable sinceFeb. 12, 2019AvailabilityAcademic Institutions and Nonprofits only -
pCAG-NLS(SV40)x2-rAPOBEC1-gs-XTEN-gs-hdAsCas12a(D908A)-gs-UGI-NLS(SV40)(AsBE1.4) (RTW1008)
Plasmid#114080PurposeCAG promoter expression plasmid for human codon optimized AsBE1.4DepositorInserthuman codon optimized DNase-inactive (D908A) enAsCas12a fused to N-terminal rAPOBEC1 and C-terminal UGI (AsBE1.4)
UseTags2x SV40 NLS and SV40 NLSExpressionMammalianMutationPromoterAvailable sinceDec. 11, 2018AvailabilityAcademic Institutions and Nonprofits only -
pCAG-rAPOBEC1-gs-XTEN-gs-hdLbCas12a(D832A)-NLS(nucleoplasmin)-gs-UGI-NLS(SV40)(LbBE1.1) (RTW1295)
Plasmid#114085PurposeCAG promoter expression plasmid for human codon optimized LbBE1.1DepositorInserthuman codon optimized DNase-inactive (D832A) LbCas12a fused to N-terminal rAPOBEC1 and C-terminal UGI (LbBE1.1)
UseTagsSV40 NLSExpressionMammalianMutationD832APromoterAvailable sinceDec. 11, 2018AvailabilityAcademic Institutions and Nonprofits only -
pCAG-rAPOBEC1-gs-XTEN-gs-hdAsCas12a(D908A)-NLS(nucleoplasmin)-gs-UGI-NLS(SV40)(AsBE1.1) (RTW1351)
Plasmid#114079PurposeCAG promoter expression plasmid for human codon optimized AsBE1.1DepositorInserthuman codon optimized DNase-inactive (D908A) enAsCas12a fused to N-terminal rAPOBEC1 and C-terminal UGI (AsBE1.1)
UseTagsSV40 NLSExpressionMammalianMutationPromoterAvailable sinceDec. 11, 2018AvailabilityAcademic Institutions and Nonprofits only -
pCAG-rAPOBEC1-gs-XTEN-gs-hdenAsCas12a(E174R/S542R/K548R/D908A)-gs-UGI-NLS(SV40)(enAsBE1.3) (RTW1296)
Plasmid#114083PurposeCAG promoter expression plasmid for human codon optimized enAsBE1.3DepositorInsertDNase-inactive (D908A) enAsCas12a (E174R/S542R/K548R) fused to N-terminal rAPOBEC1 and C-terminal UGI (enAsBE1.3)
UseTagsSV40 NLSExpressionMammalianMutationE174R, S542R, K548R and D908A, human codon optimi…PromoterAvailable sinceDec. 11, 2018AvailabilityAcademic Institutions and Nonprofits only -
pCAG-NLS(SV40)x2-rAPOBEC1-gs-XTEN-gs-hdenAsCas12a(E174R/S542R/K548R/D908A)-gs-UGI-NLS(SV40)(enAsBE1.4) (RTW1028)
Plasmid#114084PurposeCAG promoter expression plasmid for human codon optimized enAsBE1.4DepositorInsertDNase-inactive (D908A) enAsCas12a (E174R/S542R/K548R) fused to N-terminal rAPOBEC1 and C-terminal UGI (enAsBE1.4)
UseTags2x SV40 NLS and SV40 NLSExpressionMammalianMutationE174R, S542R, K548R and D908A, human codon optimi…PromoterAvailable sinceDec. 11, 2018AvailabilityAcademic Institutions and Nonprofits only -
pCAG-rAPOBEC1-gs-XTEN-gs-hdenAsCas12a(E174R/S542R/K548R/D908A)-NLS(nucleoplasmin)-gs-UGI-NLS(SV40)(enAsBE1.1) (RTW1352)
Plasmid#114081PurposeCAG promoter expression plasmid for human codon optimized enAsBE1.1DepositorInsertDNase-inactive (D908A) enAsCas12a (E174R/S542R/K548R) fused to N-terminal rAPOBEC1 and C-terminal UGI (enAsBE1.1)
UseTagsSV40 NLSExpressionMammalianMutationE174R, S542R, K548R and D908A, human codon optimi…PromoterAvailable sinceDec. 11, 2018AvailabilityAcademic Institutions and Nonprofits only -
AAV-CAG-jGCaMP8s-WPRE (AAV9)
Viral Prep#179256-AAV9PurposeReady-to-use AAV9 particles produced from AAV-CAG-jGCaMP8s-WPRE (#179256). In addition to the viral particles, you will also receive purified AAV-CAG-jGCaMP8s-WPRE plasmid DNA. CAG-driven expression of calcium sensor GCaMP8s. These AAV preparations are suitable purity for injection into animals.DepositorPromoterCAGTagsNoneAvailable sinceMay 22, 2023AvailabilityAcademic Institutions and Nonprofits only -
AAV-CAG-jGCaMP8m-WPRE (AAV9)
Viral Prep#179255-AAV9PurposeReady-to-use AAV9 particles produced from AAV-CAG-jGCaMP8m-WPRE (#179255). In addition to the viral particles, you will also receive purified AAV-CAG-jGCaMP8m-WPRE plasmid DNA. CAG-driven expression of calcium sensor GCaMP8m. These AAV preparations are suitable purity for injection into animals.DepositorPromoterCAGTagsNoneAvailable sinceMay 22, 2023AvailabilityAcademic Institutions and Nonprofits only -
AAV-CAG-jGCaMP8f-WPRE (AAV1)
Viral Prep#179254-AAV1PurposeReady-to-use AAV1 particles produced from AAV-CAG-jGCaMP8f-WPRE (#179254). In addition to the viral particles, you will also receive purified AAV-CAG-jGCaMP8f-WPRE plasmid DNA. CAG-driven expression of calcium sensor GCaMP8f. These AAV preparations are suitable purity for injection into animals.DepositorPromoterCAGTagsNoneAvailable sinceMay 18, 2023AvailabilityAcademic Institutions and Nonprofits only -
AAV-CAG-jGCaMP8f-WPRE (AAV9)
Viral Prep#179254-AAV9PurposeReady-to-use AAV9 particles produced from AAV-CAG-jGCaMP8f-WPRE (#179254). In addition to the viral particles, you will also receive purified AAV-CAG-jGCaMP8f-WPRE plasmid DNA. CAG-driven expression of calcium sensor GCaMP8f. These AAV preparations are suitable purity for injection into animals.DepositorPromoterCAGTagsNoneAvailable sinceMay 19, 2023AvailabilityAcademic Institutions and Nonprofits only -
AAV-CAG-jGCaMP8m-WPRE (AAV1)
Viral Prep#179255-AAV1PurposeReady-to-use AAV1 particles produced from AAV-CAG-jGCaMP8m-WPRE (#179255). In addition to the viral particles, you will also receive purified AAV-CAG-jGCaMP8m-WPRE plasmid DNA. CAG-driven expression of calcium sensor GCaMP8m. These AAV preparations are suitable purity for injection into animals.DepositorPromoterCAGTagsNoneAvailable sinceSept. 27, 2023AvailabilityAcademic Institutions and Nonprofits only -
AAV-CAG-jGCaMP8s-WPRE (AAV1)
Viral Prep#179256-AAV1PurposeReady-to-use AAV1 particles produced from AAV-CAG-jGCaMP8s-WPRE (#179256). In addition to the viral particles, you will also receive purified AAV-CAG-jGCaMP8s-WPRE plasmid DNA. CAG-driven expression of calcium sensor GCaMP8s. These AAV preparations are suitable purity for injection into animals.DepositorPromoterCAGTagsNoneAvailable sinceJan. 24, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCAG-NLS(SV40)x2-rAPOBEC1-gs-XTEN-gs-hdenAsCas12a(E174R/S542R/K548R/D908A)-NLS(nucleoplasmin)-gs-UGI-NLS(SV40)(enAsBE1.2)
Plasmid#114082PurposeCAG promoter expression plasmid for human codon optimized enAsBE1.2, also called RTW1348DepositorInsertDNase-inactive (D908A) enAsCas12a (E174R/S542R/K548R) fused to N-terminal rAPOBEC1 and C-terminal UGI (enAsBE1.2)
UseTags2x SV40 NLS and SV40 NLSExpressionMammalianMutationE174R, S542R, K548R and D908A, human codon optimi…PromoterAvailable sinceDec. 11, 2018AvailabilityAcademic Institutions and Nonprofits only -
AAV-CAG-jGCaMP8f-WPRE (AAV Retrograde)
Viral Prep#179254-AAVrgPurposeReady-to-use AAV Retrograde particles produced from AAV-CAG-jGCaMP8f-WPRE (#179254). In addition to the viral particles, you will also receive purified AAV-CAG-jGCaMP8f-WPRE plasmid DNA. CAG-driven expression of calcium sensor GCaMP8f. These AAV were produced with a retrograde serotype, which permits retrograde access to projection neurons. These AAV preparations are suitable purity for injection into animals.DepositorPromoterCAGTagsNoneAvailable sinceSept. 27, 2023AvailabilityAcademic Institutions and Nonprofits only -
AAV-CAG-jGCaMP8m-WPRE (AAV Retrograde)
Viral Prep#179255-AAVrgPurposeReady-to-use AAV Retrograde particles produced from AAV-CAG-jGCaMP8m-WPRE (#179255). In addition to the viral particles, you will also receive purified AAV-CAG-jGCaMP8m-WPRE plasmid DNA. CAG-driven expression of calcium sensor GCaMP8m. These AAV were produced with a retrograde serotype, which permits retrograde access to projection neurons. These AAV preparations are suitable purity for injection into animals.DepositorPromoterCAGTagsNoneAvailable sinceMarch 18, 2024AvailabilityAcademic Institutions and Nonprofits only