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pCAX-mScarlet-FBP17

Plasmid

#178366

Purpose

Expresses FBP17 c-terminal labeled with mScarelt in mammalian cells

Depositor

Erik Dent

Article

Taylor et al Life Sci Alliance. 2019 Jun 3;2(3). pii:

Insert

Formin Binding Protein 17 (FNBP1 Human)

Expression

Mammalian

Mutation

400 Glutamic Acid changed to Aspartic acid (403 A…

Promoter

Chicken Beta Actin

Available Since

March 2, 2022

Availability

Academic Institutions and Nonprofits only

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pScarlessHD-C-3x127D01-DsRed

Plasmid

#171578

Purpose

For scarless insertion of 3x127D01 NanoTag into C-term; Marked by the visible marker 3xP3-DsRed flanked by piggyBac termini.

Depositor

Norbert Perrimon, Hidde Ploegh

Article

Xu et al Elife. 2022 Jan 25;11. pii: 74326. doi:

Insert

3x127D01 (CXCR2 Human)

Expression

Insect

Available Since

June 30, 2021

Availability

Academic Institutions and Nonprofits only

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pKUNOC-ErbB1-mScarlet

Plasmid

#197360

Purpose

Encoding human ErbB1 tagged with mScarlet.

Depositor

Michiyuki Matsuda

Article

Matsuda et al J Cell Sci. 2023 Jul 31:jcs.261199. doi:

Insert

ERBB1 (EGFR Human)

Tags

mScarlet

Expression

Mammalian

Available Since

Aug. 7, 2023

Availability

Academic Institutions and Nonprofits only

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DNA-CARzeta-GFP

Plasmid

#89344

Purpose

2nd generation lentiviral vector which expresses DNA-CAR receptor fused to GFP

Depositor

Ron Vale

Article

Taylor et al Cell. 2017 Mar 23;169(1):108-119.e20. do

Insert

DNA-CARzeta (CD247, CD86 Human)

Use

Lentiviral

Tags

SNAPf tag and mEGFP

Expression

Mammalian

Available Since

June 2, 2017

Availability

Academic Institutions and Nonprofits only

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pEF-pIgH-ESCargo*

Plasmid

#140159

Purpose

Expresses a regulatable secretory cargo (without an ER export signal) for mammalian cells

Depositor

Benjamin Glick

Article

Casler et al Mol Biol Cell. 2020 Oct 28:mbcE20090591.

Insert

piGH-DsRed-Express2-FKBP(LV)(C22V)

Tags

ER signal sequence (MGWSCIILFLVATATGAHS) (N termi…

Expression

Mammalian

Promoter

EF-1a

Available Since

March 26, 2020

Availability

Academic Institutions and Nonprofits only

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pScarlessHD-N-3xVHH05-DsRed

Plasmid

#171581

Purpose

For scarless insertion of 3xVHH05 NanoTag into N-term; Marked by the visible marker 3xP3-DsRed flanked by piggyBac termini.

Depositor

Norbert Perrimon, Hidde Ploegh

Article

Xu et al Elife. 2022 Jan 25;11. pii: 74326. doi:

Insert

3xVHH05 (UBE2J1 Human)

Expression

Insect

Available Since

June 30, 2021

Availability

Academic Institutions and Nonprofits only

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YIplac211-Mup1-mScarlet2I

Plasmid

#140152

Purpose

Plasmid for pop-in/pop-out replacement of Mup1 with Mup1-mScarlet2I

Depositor

Benjamin Glick

Article

Casler et al Elife. 2020 Jun 25;9. pii: 56844. doi: 1

Insert

MUP1

Expression

Yeast

Available Since

March 25, 2020

Availability

Academic Institutions and Nonprofits only

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pLBS.EF/mScarlet(i)-hCdt100Nt

Plasmid

#129334

Purpose

Cell cycle reporter, lentivirus

Depositor

Yasushi Okada

Article

Yaginuma et al bioRxiv 2021.10.08.463131

Insert

mScarlet(i) (Addgene 85044)

Use

Lentiviral

Available Since

Oct. 15, 2021

Availability

Academic Institutions and Nonprofits only

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pcDNA3-H2A-TORCAR

Plasmid

#175016

Purpose

TORCAR targeted to the nucleus via histone H2A.

Depositor

Jin Zhang

Article

Zhou et al Nat Commun. 2020 Nov 30;11(1):6088. doi:

Insert

H2A-TORCAR

Tags

Histone H2A

Expression

Mammalian

Promoter

CMV

Available Since

Sept. 22, 2021

Availability

Academic Institutions and Nonprofits only

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pCyterm-mScarlet-I3-NCwt_N1

Plasmid

#189779

Purpose

Construct for testing oligimerization of mScarlet-I3-NCwt in mammalian cells by OSER assay

Depositor

Dorus Gadella

Article

Gadella TWJ et al Nat Methods. 2023 Apr;20(4):541-545. doi

Insert

Cyterm-mScarlet-I3-NCwt

Expression

Mammalian

Promoter

CMV

Available Since

March 21, 2023

Availability

Academic Institutions and Nonprofits only

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pSC101-GFPmut2-mScarlet-I T7

Plasmid

#208191

Purpose

frameshift mistranslation, T7

Depositor

Tanel Tenson

Article

Hinnu et al Sci Rep. 2024 Mar 22;14(1):6883. doi: 10

Inserts

GFPmut2
mScarlet-I

Use

Reporter

Expression

Bacterial

Mutation

Frameshift insertion 6TTTTTTT

Promoter

Ptet+dnaK P1

Available Since

March 25, 2024

Availability

Academic Institutions and Nonprofits only

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pSC101-GFPmut2-mScarlet-I A5

Plasmid

#208192

Purpose

frameshift mistranslation, A5

Depositor

Tanel Tenson

Article

Hinnu et al Sci Rep. 2024 Mar 22;14(1):6883. doi: 10

Inserts

GFPmut2
mScarlet-I

Use

Reporter

Expression

Bacterial

Mutation

Frameshift insertion 6AAAAA

Promoter

Ptet+dnaK P1

Available Since

March 25, 2024

Availability

Academic Institutions and Nonprofits only

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pSC101-GFPmut2-mScarlet-I A4

Plasmid

#208193

Purpose

frameshift mistranslation, A4

Depositor

Tanel Tenson

Article

Hinnu et al Sci Rep. 2024 Mar 22;14(1):6883. doi: 10

Inserts

GFPmut2
mScarlet-I

Use

Reporter

Expression

Bacterial

Mutation

Frameshift insertion 6AAAA

Promoter

Ptet+dnaK P1

Available Since

March 25, 2024

Availability

Academic Institutions and Nonprofits only

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pSC101-GFPmut2(ΔmScarlet)

Plasmid

#208185

Purpose

GFP positive, mScarlet-I negative control

Depositor

Tanel Tenson

Article

Hinnu et al Sci Rep. 2024 Mar 22;14(1):6883. doi: 10

Insert

GFPmut2

Use

Reporter

Expression

Bacterial

Promoter

Ptet+dnaK P1

Available Since

March 25, 2024

Availability

Academic Institutions and Nonprofits only

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pPOTv6-blast-His:mScarletI:His

Plasmid

#201097

Purpose

Trypanosoma brucei PCR only tagging (pPOT) vector for endogenous gene tagging

Depositor

Samuel Dean

Article

Paterou et al Open Biol. 2025 Apr;15(4):240361. doi: 1

Insert

mScarlet-I

Use

Unspecified

Promoter

read through

Available Since

July 11, 2023

Availability

Academic Institutions and Nonprofits only

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pDRESS_mTurquoise2_spatial-linker-P2A_mScarletI

Plasmid

#130510

Purpose

expresses mScarlet1

Depositor

Dorus Gadella

Article

Bindels et al Nat Protoc. 2020 Feb;15(2):450-478. doi:

Insert

mScarlet1

Expression

Mammalian

Available Since

Nov. 7, 2019

Availability

Academic Institutions and Nonprofits only

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DNA-CARtcr-IRESpuro

Plasmid

#89346

Purpose

2nd generation lentiviral vector which expresses SNAP tagged TCRbeta subunit upstream of a IRESpuro cassette

Depositor

Ron Vale

Article

Taylor et al Cell. 2017 Mar 23;169(1):108-119.e20. do

Insert

TCR beta gene (Jurkat allele) fused at the N terminus to the SNAP tag

Use

Lentiviral

Tags

SNAPf tag

Expression

Mammalian

Promoter

SFFV

Available Since

June 2, 2017

Availability

Academic Institutions and Nonprofits only

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pScarlessHD-N-3x127D01-DsRed

Plasmid

#171579

Purpose

For scarless insertion of 3x127D01 NanoTag into N-term; Marked by the visible marker 3xP3-DsRed flanked by piggyBac termini.

Depositor

Norbert Perrimon, Hidde Ploegh

Article

Xu et al Elife. 2022 Jan 25;11. pii: 74326. doi:

Insert

3x127D01 (CXCR2 Human)

Expression

Insect

Available Since

June 30, 2021

Availability

Academic Institutions and Nonprofits only

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Carl-ReNL-KDEL_pcDNA3

Plasmid

#89535

Purpose

Red color variant of bright luminescent protein, targeted to the endoplasmic reticulum

Depositor

Takeharu Nagai

Article

Suzuki et al Nat Commun. 2016 Dec 14;7:13718. doi: 10

Insert

ReNL

Tags

Calreticulin and KDEL

Expression

Mammalian

Promoter

CMV

Available Since

July 17, 2017

Availability

Academic Institutions and Nonprofits only

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DNA-CARzeta-IRESpuro

Plasmid

#89345

Purpose

2nd generation lentiviral vector which expresses DNA-CAR receptor upstream of a IRES-puro cassette

Depositor

Ron Vale

Article

Taylor et al Cell. 2017 Mar 23;169(1):108-119.e20. do

Insert

DNA-CARzeta

Use

Lentiviral

Tags

SNAPf tag

Expression

Mammalian

Available Since

June 2, 2017

Availability

Academic Institutions and Nonprofits only

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