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GFP-BAZ1B

Plasmid

#65372

Purpose

GFP-tagged BRD protein, which can be used to be expressed in mammalian cells.

Depositor

Kyle Miller

Article

Gong et al Genes Dev. 2015 Jan 15;29(2):197-211. do

Insert

BAZ1B (BAZ1B Human)

Tags

EmGFP and V5

Expression

Mammalian

Mutation

none

Promoter

CMV

Available Since

June 8, 2015

Availability

Academic Institutions and Nonprofits only

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pCAG RpH-LAMP1-3xFLAG

Plasmid

#163018

Purpose

expresses ratiometric sensor of lysosomal lumenal pH

Depositor

Massimiliano Stagi

Article

Ponsford et al Autophagy. 2020 Jun 9:1-19. doi: 10.1080

Insert

RpH-LAMP1-3xFLAG (Lamp1 Mouse)

Tags

3XFLAG

Expression

Mammalian

Available Since

Dec. 7, 2020

Availability

Academic Institutions and Nonprofits only

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AAV-CMV-Nrf2

Plasmid

#67636

Purpose

AAV vector expressing Nrf2 gene

Depositor

Connie Cepko

Article

Xiong et al J Clin Invest. 2015 Apr;125(4):1433-45.

Insert

Nrf2 (Nfe2l2 Mouse)

Use

AAV

Promoter

CMV

Available Since

Aug. 24, 2015

Availability

Academic Institutions and Nonprofits only

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GFP-BRD7

Plasmid

#65379

Purpose

GFP-tagged BRD protein, which can be used to be expressed in mammalian cells.

Depositor

Kyle Miller

Article

Gong et al Genes Dev. 2015 Jan 15;29(2):197-211. do

Insert

BRD7 (BRD7 Human)

Tags

EmGFP and V5

Expression

Mammalian

Mutation

none

Promoter

CMV

Available Since

May 14, 2015

Availability

Academic Institutions and Nonprofits only

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pSB1C3-J23110-B0034-mRFP1_Magenta

Plasmid

#160446

Purpose

BioBrick pSB1C3 plasmid that constitutively overexpresses mRFP1_Magenta chromoprotein in E. coli

Depositor

Anthony Forster

Article

Bao et al Anal Biochem. 2020 Sep 3:113936. doi: 10

Insert

promoter, RBS, mRFP1E_Magenta

Use

Synthetic Biology

Mutation

BioBrick sites removed

Available Since

Jan. 20, 2021

Availability

Academic Institutions and Nonprofits only

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Nano-lantern/pcDNA3

Plasmid

#51970

Purpose

mammalian expression of Nano-lantern, a luminescent protein for high-speed single-cell and whole body imaging

Depositor

Takeharu Nagai

Article

Saito et al Nat Commun. 2012;3:1262. doi: 10.1038/nc

Insert

Nano-lantern

Expression

Mammalian

Mutation

S257G in Rluc8

Available Since

March 18, 2014

Availability

Academic Institutions and Nonprofits only

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pCSII-EF-miRFP709-hCdt(1/100)

Plasmid

#80007

Purpose

hCdt(1/100) fused with near-infrared fluorescent protein miRFP709 (G1 phase cell cycle reporter)

Depositor

Vladislav Verkhusha

Article

Shcherbakova et al Nat Commun. 2016 Aug 19;7:12405. doi: 10

Insert

hCdt1(1/100) (CDT1 Human)

Use

Lentiviral

Tags

miRFP709

Promoter

EF-1 alpha

Available Since

July 21, 2016

Availability

Academic Institutions and Nonprofits only

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pMito-rxRFP1.1

Plasmid

#67841

Purpose

redox-sensitive red fluorescent protein rxRFP1.1, mitochondrially localized

Depositor

Huiwang Ai

Article

Fan et al Anal Bioanal Chem. 2016 Jan 12.

Insert

rxRFP1.1 and mitochondrial localization sequence

Tags

His Tag and mitochondrial localization sequence

Expression

Mammalian

Promoter

CMV

Available Since

Oct. 9, 2015

Availability

Academic Institutions and Nonprofits only

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pmtb-t7-alpha-bungarotoxin

Plasmid

#69542

Purpose

For in vitro transcription of alpha-bungarotoxin mRNA that can be injected into embryos.

Depositor

Sean Megason

Article

Swinburne et al PLoS One. 2015 Aug 5;10(8):e0134005. doi

Insert

alpha-bungarotoxin

Use

Tol2, beta-actin promoter, sp6,t7 for zebrafish

Promoter

beta-actin, sp6, t7

Available Since

Nov. 3, 2015

Availability

Academic Institutions and Nonprofits only

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1GFP/RNase H1 WT

Plasmid

#174447

Purpose

Express GFP-tagged RNase H1 WT in E. coli.

Depositor

Karlene Cimprich

Article

Crossley et al J Cell Biol. 2021 Sep 6;220(9). pii: 212

Insert

RNase H1 (RNASEH1 Human)

Tags

His-tag, GFP

Expression

Bacterial

Promoter

T7 promoter

Available Since

Sept. 7, 2021

Availability

Academic Institutions and Nonprofits only

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pRetroX-Tight-MCS_PGK-OgNLuc

Plasmid

#70186

Purpose

Constiutive mammalian expression of the bioluminescent OgNLuc LumiFluor reporter for single-cell analysis and sensitive optical imaging of living subjects.

Depositor

Antonio Amelio

Article

Schaub et al Cancer Res. 2015 Sep 30. pii: canres.353

Type

Empty backbone

Use

Retroviral

Expression

Mammalian

Promoter

PGK

Available Since

Dec. 2, 2015

Availability

Academic Institutions and Nonprofits only

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Halo-KDEL

Plasmid

#124316

Purpose

Expresses ER specific HaloTag protein

Depositor

Jin Wang

Article

Jiang et al Antioxid Redox Signal. 2018 Oct 25. doi:

Insert

Halo tag

Tags

KDEL Seq

Expression

Mammalian

Promoter

CMV

Available Since

May 16, 2019

Availability

Academic Institutions and Nonprofits only

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GFP-TRIM28

Plasmid

#65397

Purpose

GFP-tagged BRD protein, which can be used to be expressed in mammalian cells.

Depositor

Kyle Miller

Article

Gong et al Genes Dev. 2015 Jan 15;29(2):197-211. do

Insert

TRIM28 (TRIM28 Human)

Tags

EmGFP and V5

Expression

Mammalian

Mutation

none

Promoter

CMV

Available Since

May 13, 2015

Availability

Academic Institutions and Nonprofits only
pPUR-hU6-Sirius-8XMS2-mU6-Sirius-8XPP7

Plasmid

#121944

Purpose

CRISPR-Sirius plasmid

Depositor

Thoru Pederson

Article

Ma et al Nat Methods. 2018 Nov;15(11):928-931. do

Insert

hU6-Sirius-8XMS2-mU6-Sirius-8XPP7 Cassette

Use

CRISPR and Lentiviral

Tags

no

Expression

Mammalian

Promoter

U6 promoters

Available Since

Feb. 12, 2019

Availability

Academic Institutions and Nonprofits only

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pBH-UAS-secA5-YFP

Plasmid

#32359

Purpose

Live imaging of apoptotic cells in zebrafish

Depositor

Randall Peterson

Article

van Ham et al FASEB J. 2010 Nov;24(11):4336-42. Epub 2

Insert

secreted human annexin 5 (ANXA5 Human)

Use

Zebrafish expression

Promoter

UAS

Available Since

Sept. 19, 2011

Availability

Academic Institutions and Nonprofits only

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pCI_mini-mAgrin-AviTag

Plasmid

#198131

Purpose

Express mini-Agrin in mammalian cells

Depositor

Jeffrey Miner

Insert

mini-Agrin (Agrn Mouse)

Tags

TEV-Myc-His

Expression

Mammalian

Promoter

CMV, beta-globin/IgG chimeric intron

Available Since

June 2, 2023

Availability

Academic Institutions and Nonprofits only

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mRFP-RBD(R59A)-mRFP

Plasmid

#18664

Depositor

Karel Svoboda

Article

Yasuda et al Nat Neurosci. 2006 Feb . 9(2):283-91.

Insert

Ras binding domain of Raf

Tags

mRFP

Expression

Mammalian

Mutation

R59A mutation in RBD

Available Since

July 2, 2008

Availability

Academic Institutions and Nonprofits only

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pLX_lenti_osTir1_9Myc_P2A_Bsd

Plasmid

#139746

Purpose

lentiviral vector expressing rice E3 ligase Tir1 in mammalian cells with selection marker

Depositor

Robert Tjian

Article

Xie et al Nat Methods. 2020 Apr;17(4):430-436. doi

Insert

rice E3 ligase Tir1 (osTir1)

Expression

Mammalian

Promoter

EF1alpha

Available Since

April 17, 2020

Availability

Academic Institutions and Nonprofits only

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pBAD/HisB-iRFP670

Plasmid

#45453

Depositor

Vladislav Verkhusha

Article

Shcherbakova et al Nat Methods. 2013 Jun 16. doi: 10.1038/n

Insert

iRFP670

Expression

Bacterial

Mutation

T2A, S45T, V116I, D124E, M170L, K180M, D202L, I20…

Promoter

araBAD

Available Since

June 17, 2013

Availability

Academic Institutions and Nonprofits only

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pCS-memb-mCherry

Plasmid

#53750

Purpose

Contains mCherry fused to a 2x membrane localization sequence in the pCS2+ vector.

Depositor

Sean Megason

Article

Megason et al Methods Mol Biol. 2009;546:317-32. doi:

Insert

2x membrane linker

Tags

mCherry

Expression

Mammalian

Promoter

simian CMV IE94

Available Since

June 10, 2014

Availability

Academic Institutions and Nonprofits only

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